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4. Oropharyngeal and nasopharyngeal decontamination with chlorhexidine gluconate in lung cancer surgery: a randomized clinical trial

Author

D’Journo, Xavier Benoit, Falcoz, Pierre-Emmanuel, Alifano, Marco, Le Rochais, Jean-Philippe, D’Annoville, Thomas, Massard, Gilbert, Regnard, Jean Francois, Icard, Philippe, Marty-Ane, Charles, Trousse, Delphine, Doddoli, Christophe, Orsini, Bastien, Edouard, Sophie, Million, Matthieu, Lesavre, Nathalie, Loundou, Anderson, Baumstarck, Karine, Peyron, Florence, Honoré, Stephane, Dizier, Stéphanie, Charvet, Aude, Leone, Marc, Raoult, Didier, Papazian, Laurent, and Thomas, Pascal Alexandre

Published
2018
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6. Harnessing the Vnn1 pantetheinase pathway boosts short chain fatty acids production and mucosal protection in colitis

Author

Millet, Virginie, primary, Gensollen, Thomas, additional, Maltese, Michael, additional, Serrero, Melanie, additional, Lesavre, Nathalie, additional, Bourges, Christophe, additional, Pitaval, Christophe, additional, Cadra, Sophie, additional, Chasson, Lionel, additional, Vu Man, Thien Phong, additional, Masse, Marion, additional, Martinez-Garcia, Juan Jose, additional, Tranchida, Fabrice, additional, Shintu, Laetitia, additional, Mostert, Konrad, additional, Strauss, Erick, additional, Lepage, Patricia, additional, Chamaillard, Mathias, additional, Broggi, Achille, additional, Peyrin-Biroulet, Laurent, additional, Grimaud, Jean-Charles, additional, Naquet, Philippe, additional, and Galland, Franck, additional

Published
2022
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8. Harnessing the Vnn1 pantetheinase pathway boosts short chain fatty acids production and mucosal protection in colitis.

Author

Millet, Virginie, Gensollen, Thomas, Maltese, Michael, Serrero, Melanie, Lesavre, Nathalie, Bourges, Christophe, Pitaval, Christophe, Cadra, Sophie, Chasson, Lionel, Thien Phong Vu Man, Masse, Marion, Martinez-Garcia, Juan Jose, Tranchida, Fabrice, Shintu, Laetitia, Mostert, Konrad, Strauss, Erick, Lepage, Patricia, Chamaillard, Mathias, Broggi, Achille, and Peyrin-Biroulet, Laurent

Subjects
BUTYRATES, SHORT-chain fatty acids, COLITIS, INFLAMMATORY bowel diseases, PANTOTHENIC acid, HYPOKINESIA
Published
2023
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9. Intracoronary administration of darbepoetin-alpha at onset of reperfusion in acute myocardial infarction: Results of the randomized Intra-Co-EpoMI trial

Author

Roubille, François, Micheau, Antoine, Combes, Stéphane, Thibaut, Séverine, Souteyrand, Géraud, Cayla, Guillaume, Bonello, Laurent, Lesavre, Nathalie, Sportouch-Dukhan, Catherine, Klein, François, Berboucha, Samir, Cade, Stéphane, Cung, Thien-Tri, Raczka, Franck, Macia, Jean-Christophe, Gervasoni, Richard, Cransac, Frédéric, Leclercq, Florence, Barrère-Lemaire, Stéphanie, Paganelli, Franck, Mottref, Pascal, Vernhet Kovacsik, Hélène, Ovize, Michel, and Piot, Christophe

Published
2013
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10. Impact of Neuroeffector Adrenergic Receptor Polymorphisms on Incident Ventricular Fibrillation During Acute Myocardial Ischemia.

Author

Chevalier, Philippe, Roy, Pascal, Bessière, Francis, Morel, Elodie, Ankou, Bénédicte, Morgan, Gina, Halder, Indrani, London, Barry, Minobe, Wayne A., Slavov, Dobromir, Delinière, Antoine, Bochaton, Thomas, Paganelli, Franck, Lesavre, Nathalie, Boiteux, Clément, Mansourati, Jacques, Maury, Philippe, Clerici, Gaël, Winum, Pierre François, and Huebler, Sophia P.

Published
2023
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12. Identification of Cx43 variants predisposing to ventricular fibrillation in the acute phase of ST-elevation myocardial infarction

Author

Chevalier, Philippe, Moreau, Adrien, Bessière, Francis, Richard, Sylvain, Chahine, Mohamed, Millat, Gilles, Morel, Elodie, Paganelli, Franck, Lesavre, Nathalie, Placide, Leslie, Montestruc, François, Ankou, Bénédicte, Puertas, Rosa Doñate, Asatryan, Babken, Delinière, Antoine, MORNET, Dominique, Institut NeuroMyoGène (INMG), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Groupement Hospitalier Lyon-Est (GHE), Hospices Civils de Lyon (HCL), Hôpital Louis Pradel [CHU - HCL], Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046] (PhyMedExp), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Hôpital Arnaud de Villeneuve [CHRU Montpellier], Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Centre de recherche de l’Institut universitaire en santé mentale de Québec [Canada] (CERVO), Département de réadaptation (Faculté de médecine de l'Université Laval) [Canada], Faculté de médecine de l'Université Laval [Québec] (ULaval), Université Laval [Québec] (ULaval)-Université Laval [Québec] (ULaval)-Faculté de médecine de l'Université Laval [Québec] (ULaval), Université Laval [Québec] (ULaval)-Université Laval [Québec] (ULaval), Centre de Biologie et Pathologie Est (CBPE), Hospices Civils de Lyon (HCL)-Centre National de Référence des Légionelles, Hôpital Nord [CHU - APHM], eXYSTAT [Malakoff], Signalisation et physiopathologie cardiovasculaire (CARPAT), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Saclay, and Bern University Hospital [Berne] (Inselspital)

Subjects
[SDV.GEN.GPO]Life Sciences [q-bio]/Genetics/Populations and Evolution [q-bio.PE], Acute ventricular fibrillation, [SDV.GEN.GH] Life Sciences [q-bio]/Genetics/Human genetics, ST-elevation myocardial infarction (STEMI), [SDV.MHEP.CSC] Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, GJA1 variants, Sudden cardiac death, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, [SDV.GEN.GH]Life Sciences [q-bio]/Genetics/Human genetics, Physiology (medical), Connexin 43, [SDV.GEN.GPO] Life Sciences [q-bio]/Genetics/Populations and Evolution [q-bio.PE], Cardiology and Cardiovascular Medicine, 610 Medizin und Gesundheit
Abstract

Aims Ventricular fibrillation (VF) occurring in the acute phase of ST-elevation myocardial infarction (STEMI) is the leading cause of sudden cardiac death worldwide. Several studies showed that reduced connexin 43 (Cx43) expression and reduced conduction velocity increase the risk of VF in acute myocardial infarction (MI). Furthermore, genetic background might predispose individuals to primary VF (PVF). The primary objective was to evaluate the presence of GJA1 variants in STEMI patients. The secondary objective was to evaluate the arrhythmogenic impact of GJA1 variants in STEMI patients with VF. Methods and results The MAP-IDM prospective cohort study included 966 STEMI patients and was designed to identify genetic predisposition to VF. A total of 483 (50.0%) STEMI patients with PVF were included. The presence of GJA1 variants increased the risk of VF in STEMI patients [from 49.1 to 70.8%, P = 0.0423; odds ratio (OR): 0.40; 95% confidence interval: 0.16–0.97; P = 0.04]. The risk of PVF decreased with beta-blocker intake (from 53.5 to 44.8%, P = 0.0085), atrial fibrillation (from 50.7 to 26.4%, P = 0.0022), and with left ventricular ejection fraction >50% (from 60.2 to 41.4%, P < 0.0001). Among 16 GJA1 variants, three novel heterozygous missense variants were identified in three patients: V236I, H248R, and I327M. In vitro studies of these variants showed altered Cx43 localization and decreased cellular communication, mainly during acidosis. Conclusion Connexin 43 variants are associated with increased VF susceptibility in STEMI patients. Restoring Cx43 function may be a potential therapeutic target to prevent PVF in patients with acute MI. Clinical trial registration Clinical Trial Registration: https://clinicaltrials.gov/ct2/show/NCT00859300

Published
2022
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13. Grandmaternal cells in cord blood

Author

Karlmark, Karlin R., Haddad, Marina El, Donato, Xavier-Côme, Martin, Gabriel V., Bretelle, Florence, Lesavre, Nathalie, Cocallemen, Jean-François, Martin, Marielle, Picard, Christophe, Albentosa, Tiffany, Roudier, Jean, Desbriere, Raoul, and Lambert, Nathalie C.

Subjects
Adult, Medicine (General), HLA compatibility, Cord blood, Aneuploidy, Fetal Blood, Chimerism, Healthy Volunteers, Pedigree, Grandparents, three generations, R5-920, HLA Antigens, Pregnancy, Humans, Medicine, Female, France, Maternal Inheritance, grandmaternal microchimerism, Maternal-Fetal Exchange, Research Paper, Maternal Age
Abstract

s: Background: During pregnancy a feto-maternal exchange of cells through the placenta conducts to maternal microchimerism (Mc) in the child and fetal Mc in the mother. Because of this bidirectional traffic of cells, pregnant women have also acquired maternal cells in utero from their mother and could transfer grandmaternal (GdM) cells to their child through the maternal bloodstream during pregnancy. Thus, cord blood (CB) samples could theoretically carry GdMMc. Nevertheless this has never been demonstrated. Methods: Using Human Leukocyte Antigen (HLA)-specific quantitative PCR assays on three-generation families, we were able to test 28 CB samples from healthy primigravid women for GdMMc in whole blood (WB) and isolated cells (PBMC, T, B, granulocytes, stem cells). Findings: Five CB samples (18%) had GdMMc which could not be confounded with maternal source, with quantities 100 fold lower than maternal Mc in WB and PBMC. Risk of aneuploidies and/or related invasive prenatal procedures significantly correlated with the presence of GdMMc in CB (p=0.024). Significantly decreased HLA compatibility was observed in three-generation families from CB samples carrying GdMMc (p=0.019). Interpretation: Transgenerational transfer of cells could have implications in immunology and evolution. Further analyses will be necessary to evaluate whether GdMMc in CB is a passive or immunologically active transfer and whether invasive prenatal procedures could trigger GdMMc. Funding: Provence-Alpes-Côte d'Azur APEX grant # 2012_06549E, 2012_11786F and 2014_03978) and the Foundation for Medical Research (FRM Grant #ING20140129045).

Published
2021

14. Identification of Cx43 variants predisposing to ventricular fibrillation in the acute phase of ST-elevation myocardial infarction.

Author

Chevalier, Philippe, Moreau, Adrien, Bessière, Francis, Richard, Sylvain, Chahine, Mohamed, Millat, Gilles, Morel, Elodie, Paganelli, Franck, Lesavre, Nathalie, Placide, Leslie, Montestruc, François, Ankou, Bénédicte, Puertas, Rosa Doñate, Asatryan, Babken, Delinière, Antoine, and Investigators, MAP-IDM

Abstract

Aims Ventricular fibrillation (VF) occurring in the acute phase of ST-elevation myocardial infarction (STEMI) is the leading cause of sudden cardiac death worldwide. Several studies showed that reduced connexin 43 (Cx43) expression and reduced conduction velocity increase the risk of VF in acute myocardial infarction (MI). Furthermore, genetic background might predispose individuals to primary VF (PVF). The primary objective was to evaluate the presence of GJA1 variants in STEMI patients. The secondary objective was to evaluate the arrhythmogenic impact of GJA1 variants in STEMI patients with VF. Methods and results The MAP-IDM prospective cohort study included 966 STEMI patients and was designed to identify genetic predisposition to VF. A total of 483 (50.0%) STEMI patients with PVF were included. The presence of GJA1 variants increased the risk of VF in STEMI patients [from 49.1 to 70.8%, P = 0.0423; odds ratio (OR): 0.40; 95% confidence interval: 0.16–0.97; P = 0.04]. The risk of PVF decreased with beta-blocker intake (from 53.5 to 44.8%, P = 0.0085), atrial fibrillation (from 50.7 to 26.4%, P = 0.0022), and with left ventricular ejection fraction >50% (from 60.2 to 41.4%, P < 0.0001). Among 16 GJA1 variants, three novel heterozygous missense variants were identified in three patients: V236I, H248R, and I327M. In vitro studies of these variants showed altered Cx43 localization and decreased cellular communication, mainly during acidosis. Conclusion Connexin 43 variants are associated with increased VF susceptibility in STEMI patients. Restoring Cx43 function may be a potential therapeutic target to prevent PVF in patients with acute MI. Clinical trial registration Clinical Trial Registration: https://clinicaltrials.gov/ct2/show/NCT00859300 [ABSTRACT FROM AUTHOR]

Published
2023
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15. Corrigendum: Factors Predicting the Presence of Maternal Cells in Cord Blood and Associated Changes in Immune Cell Composition

Author

Haddad, Marina El, primary, Karlmark, Karlin, additional, Donato, Xavier-Côme, additional, Martin, Gabriel, additional, Bretelle, Florence, additional, Lesavre, Nathalie, additional, Cocallemen, Jean-François, additional, Martin, Marielle, additional, Picard, Christophe, additional, Roudier, Jean, additional, Desbriere, Raoul, additional, and Lambert, Nathalie C., additional

Published
2021
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16. Long-term outcome after EUS-guided radiofrequency ablation: Prospective results in pancreatic neuroendocrine tumors and pancreatic cystic neoplasms

Author

Barthet, Marc, additional, Giovannini, Marc, additional, Gasmi, Mohamed, additional, Lesavre, Nathalie, additional, Boustière, Christian, additional, Napoleon, Bertrand, additional, LaQuiere, Arthur, additional, Koch, Stephane, additional, Vanbiervliet, Geoffroy, additional, and Gonzalez, Jean-Michel, additional

Published
2021
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18. Factors Predicting the Presence of Maternal Cells in Cord Blood and Associated Changes in Immune Cell Composition

Author

Haddad, Marina El, primary, Karlmark, Karlin R., additional, Donato, Xavier-Côme, additional, Martin, Gabriel V., additional, Bretelle, Florence, additional, Lesavre, Nathalie, additional, Cocallemen, Jean-François, additional, Martin, Marielle, additional, Picard, Christophe, additional, Roudier, Jean, additional, Desbriere, Raoul, additional, and Lambert, Nathalie C., additional

Published
2021
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21. Sa1398 EUS-GUIDED RADIOFREQUENCY ABLATION: LONG-TERM OUTCOME RESULTS FOR PANCREATIC NEUROENDOCRINE TUMORS AND PANCREATIC CYSTIC NEOPLASMS IN A PROSPECTIVE MULTICENTER STUDY

Author

Barthet, Marc, primary, Giovannini, Marc, additional, Lesavre, Nathalie, additional, Boustiere, Christian, additional, Napoleon, Bertrand, additional, Koch, Stephane, additional, Vanbiervliet, Geoffroy, additional, Gonzalez, Jean-Michel, additional, and Gasmi, Mohamed, additional

Published
2020
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22. Incidence of Venous Thromboembolism in Patients With Newly Diagnosed Pancreatic Cancer and Factors Associated With Outcomes

Author

Frere, Corinne, primary, Bournet, Barbara, additional, Gourgou, Sophie, additional, Fraisse, Julien, additional, Canivet, Cindy, additional, Connors, Jean M., additional, Buscail, Louis, additional, Farge, Dominique, additional, Carrère, Nicolas, additional, Muscari, Fabrice, additional, Suc, Bertrand, additional, Guimbaud, Rosine, additional, Couteau, Corinne, additional, Deslandres, Marion, additional, Rivera, Pascale, additional, Laurenty, Anne-Pascale, additional, Fares, Nadim, additional, Barange, Karl, additional, Selves, Janick, additional, Gomez-Brouchet, Anne, additional, Napoléon, Bertrand, additional, Pujol, Bertrand, additional, Fumex, Fabien, additional, Desrame, Jérôme, additional, Lefort, Christine, additional, Lepilliez, Vincent, additional, Gincul, Rodica, additional, Artru, Pascal, additional, Clavel, Léa, additional, Lemaistre, Anne-Isabelle, additional, Palazzo, Laurent, additional, Cros, Jérôme, additional, Tubiana, Sarah, additional, Flori, Nicolas, additional, Senesse, Pierre, additional, Colombo, Pierre-Emmanuel, additional, Samail-Scalzi, Emmanuelle, additional, Portales, Fabienne, additional, Ga, Claire Honfo, additional, Plassot, Carine, additional, Bibeau, Frédéric, additional, Ychou, Marc, additional, Guibert, Pierre, additional, Fouchardière, Christelle de la, additional, Sarabi, Matthieu, additional, Peyrat, Patrice, additional, Tabone-Eglinger, Séverine, additional, Renard, Caroline, additional, Piessen, Guillaume, additional, Truant, Stéphanie, additional, Saudemont, Alain, additional, Millet, Guillaume, additional, Renaud, Florence, additional, Leteurtre, Emmanuelle, additional, Gele, Patrick, additional, Assenat, Eric, additional, Fabre, Jean-Michel, additional, Souche, François-Régis, additional, Dupuy, Marie, additional, Gorce-Dupuy, Anne-Marie, additional, Ramos, Jeanne, additional, Seitz, Jean-François, additional, Hardwigsen, Jean, additional, Norguet-Monnereau, Emmanuelle, additional, Grandval, Philippe, additional, Duluc, Muriel, additional, Figarella-Branger, Dominique, additional, Vendrely, Véronique, additional, Subtil, Clément, additional, Terrebonne, Eric, additional, Blanc, Jean-Frédéric, additional, Buscail, Etienne, additional, Merlio, Jean-Philippe, additional, Gornet, Jean-Marc, additional, Geromin, Daniela, additional, Vanbiervliet, Geoffroy, additional, Frin, Anne-Claire, additional, Ouvrier, Delphine, additional, Saint-Paul, Marie-Christine, additional, Berthelémy, Philippe, additional, Fouad, Chelbabi, additional, Garcia, Stéphane, additional, Lesavre, Nathalie, additional, Gasmi, Mohamed, additional, Barthet, Marc, additional, Cottet, Vanessa, additional, and Delpierre, Cyrille, additional

Published
2020
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23. Radiology and artificial intelligence: An opportunity for our specialty

Author

D'Journo, Xavier Benoit, Falcoz, Pierre-Emmanuel, Alifano, Marco, Le Rochais, Jean-Philippe, D'Annoville, Thomas, Massard, Gilbert, Regnard, Jean Francois, Icard, Philippe, Marty-Ane, Charles, Trousse, Delphine, Doddoli, Christophe, Orsini, Bastien, Edouard, Sophie, Million, Matthieu, Lesavre, Nathalie, Loundou, Anderson, Baumstarck, Karine, Peyron, Florence, Honore, Stephane, Dizier, Stephanie, Charvet, Aude, Leone, Marc, Raoult, Didier, Papazian, Laurent, Thomas, Pascal Alexandre, Centre Hospitalier Universitaire de Nîmes (CHU Nîmes), Hôpital Paul Brousse, service de neuroradiologie [Paris], Hôpital Sainte-Anne, Service de chirurgie thoracique et cardio-vasculaire, Université Montpellier 1 (UM1)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Hôpital Arnaud de Villeneuve-Université de Montpellier (UM), Microbes évolution phylogénie et infections (MEPHI), and Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)

Subjects
medicine.medical_specialty, Radiological and Ultrasound Technology, business.industry, [SDV]Life Sciences [q-bio], Specialty, MEDLINE, General Medicine, 030218 nuclear medicine & medical imaging, 03 medical and health sciences, 0302 clinical medicine, Artificial Intelligence, 030220 oncology & carcinogenesis, Medicine, Radiology, Nuclear Medicine and imaging, Medical physics, business, Radiology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2018

25. A Combination of Single-Nucleotide Polymorphisms Is Associated with the Interindividual Variability in VitaminD Bioavailability in Healthy Men

Author

Desmarchelier, Charles, Reboul, Emmanuelle, Goncalves, Aurélie, Kopec, Rachel, Nowicki, Marion, Morange, Sophie, Lesavre, Nathalie, Portugal, Henri, Borel, Patrick, Nutrition, obésité et risque thrombotique (NORT), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Sécurité et Qualité des Produits d'Origine Végétale (SQPOV), Institut National de la Recherche Agronomique (INRA)-Avignon Université (AU), Clinical Investigation Centre, Hôpital de la Conception [CHU - APHM] (LA CONCEPTION ), Hôpital Nord, Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Hôpital de la Conception [CHU - APHM] (LA CONCEPTION), Société Française des Vitamines et Biofacteurs (SFVB). FRA., Avignon Université (AU)-Institut National de la Recherche Agronomique (INRA), Centre Hospitalier Universitaire de Saint-Etienne [CHU Saint-Etienne] (CHU ST-E), and ProdInra, Archive Ouverte

Subjects
snps, polymorphisme nucléotidique simple (SNP), métabolisme lipidique, 25-hydroxycholecalciferol, nutragenetics, dihydroxy cholécalciférol, vitamine d, vitamin d, polymorphisme, polymorphism, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, biodisponibilité, lipid metabolism, lipids (amino acids, peptides, and proteins), polymorphisme mononucléotidique, bioavailability, absorption, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, nutrigenetics, nutrigénétique
Abstract

Background – A significant part of vitamin D present in the human body is synthesized in the skin following UV light exposition. However, to obtain or maintain an adequate vitamin D status, most individuals have to consume vitamin D, either as part of their diet or as supplements. The blood response to vitamin D supplementation has been shown to exhibit a large interindividual variability. Moreover, vitamin D intestinal transport depends, at least partly, on proteins and we have shown that the bioavailability of other lipid micronutrients (tocopherol, lutein, lycopene and beta-carotene), which share common absorption mechanism with cholecalciferol (D3),was partly modulated by single nucleotide polymorphisms (SNPs) in genes involved in the intestinal transport of these micronutrients and in the metabolism of chylomicrons. The main objective of this study was thus to identify SNPs associated with the interindividual variability in the bioavailability of D3. Methods – Thirty nine healthy adult men were genotyped using whole-genome microarrays. They consumed a meal that contained 5g D3 as a supplement. D3 and calcifediol (25(OH)D) concentration in plasma chylomicrons was measured at regular time intervals up to 8h after meal intake. The association between SNPs in genes involved in vitamin D and lipid metabolism (61 candidate genes representing 3791 SNPs) and the D3 response (calculated as the area-under-the-curve of the postprandial chylomicron D3 concentration) was analysed by partial least squares regression (PLS regression).Results – The postprandial chylomicron D3 concentration peaked 5.4 h after meal intake. There was no significant variation in the postprandial chylomicron 25(OH)D concentration. The D3 response displayed a large interindividual variability (CV=47%). It correlated positively with the postprandial chylomicron triglyceride response (r=0.60, P

Published
2017

26. Additional file 2: Figure S1. of Increased serum levels of fractalkine and mobilisation of CD34+CD45− endothelial progenitor cells in systemic sclerosis

Author

Benyamine, Audrey, Magalon, Jérémy, Cointe, Sylvie, Lacroix, Romaric, Arnaud, Laurent, Bardin, Nathalie, Rossi, Pascal, Francès, Yves, Bernard-Guervilly, Fanny, Kaplanski, Gilles, Jean-Robert Harlé, Pierre-Jean Weiller, Berbis, Philippe, Braunstein, David, Jouve, Elisabeth, Lesavre, Nathalie, Couranjou, Françoise, Dignat-George, Françoise, Sabatier, Florence, Paul, Pascale, and Granel, Brigitte

Abstract

Gating strategy of endothelial progenitor cells (EPCs) by flow cytometry. CD34+CD45+ haematopoietic progenitor cells (HPCs) and CD34+CD45− EPCs were identified within 7-AAD-negative viable (a), CD34+ cells (b), with CD45dim or CD45− expression (c), and displaying forward scatter (FS)/side scatter (SS) characteristics corresponding to the lymphocyte cluster (d, e). Non-specific binding of CD34-PE antibody is checked on CD34+CD45+ (f) and CD34+CD45− gate of each sample (g) by means of a control tube in which CD34-PE antibody is replaced by its isoclonic control. LYMPH Lymphocytes. Figure S2. CFU-EC count in the peripheral venous blood of healthy control subjects (HC) and patients with systemic sclerosis (SSc). Number (n) of CFU-ECs was determined after cell culture of MNCs. ** P

Published
2017
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27. Additional file 1: Table S1. of Increased serum levels of fractalkine and mobilisation of CD34+CD45− endothelial progenitor cells in systemic sclerosis

Author

Benyamine, Audrey, Magalon, Jérémy, Cointe, Sylvie, Lacroix, Romaric, Arnaud, Laurent, Bardin, Nathalie, Rossi, Pascal, Francès, Yves, Bernard-Guervilly, Fanny, Kaplanski, Gilles, Jean-Robert Harlé, Pierre-Jean Weiller, Berbis, Philippe, Braunstein, David, Jouve, Elisabeth, Lesavre, Nathalie, Couranjou, Françoise, Dignat-George, Françoise, Sabatier, Florence, Paul, Pascale, and Granel, Brigitte

Subjects
integumentary system, skin and connective tissue diseases
Abstract

Ongoing treatments in patients with SSc. Table S2. Biological characteristics of the study population. Table S3. Summary of the main immunophenotypic and functional characteristics of the investigated circulating progenitor cell subsets. Table S4. Multivariate logistic regression analysis for SSc with robust estimator. (DOCX 16 kb)

Published
2017
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28. p8 inhibits the growth of human pancreatic cancer cells and its expression is induced through pathways involved in growth inhibition and repressed by factors promoting cell growth

Author

Vasseur Sophie, Lesavre Nathalie, Malicet Cédric, and Iovanna Juan L

Subjects
p8, pancreatic cancer, ras, TGFβ-1, p38, JNK., Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
Abstract

Abstract Background p8 is a stress-induced protein with multiple functions and biochemically related to the architectural factor HMG-I/Y. We analyzed the expression and function of p8 in pancreatic cancer-derived cells. Methods Expression of p8 was silenced in the human pancreatic cancer cell lines Panc-1 and BxPc-3 by infection with a retrovirus expressing p8 RNA in the antisense orientation. Cell growth was measured in control and p8-silenced cells. Influence on p8 expression of the induction of intracellular pathways promoting cellular growth or growth arrest was monitored. Results p8-silenced cells grew more rapidly than control cells transfected with the empty retrovirus. Activation of the Ras→Raf→MEK→ERK and JNK intracellular pathways down-regulated p8 expression. In addition, the MEK1/2 inhibitor U0126 and the JNK inhibitor SP600125 up-regulates expression of p8. Conversely, p38 or TGFβ-1 induced p8 expression whereas the specific p38 inhibitor SB203580 down-regulated p8 expression. Finally, TGFβ-1 induction was in part mediated through p38. Conclusions p8 inhibits the growth of human pancreatic cancer cells. p8 expression is induced through pathways involved in growth inhibition and repressed by factors that promote cell growth. These results suggest that p8 belongs to a pathway regulating the growth of pancreatic cancer cells.

Published
2003
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29. Increased serum levels of fractalkine and mobilisation of CD34+CD45− endothelial progenitor cells in systemic sclerosis

Author

Benyamine, Audrey, primary, Magalon, Jérémy, additional, Cointe, Sylvie, additional, Lacroix, Romaric, additional, Arnaud, Laurent, additional, Bardin, Nathalie, additional, Rossi, Pascal, additional, Francès, Yves, additional, Bernard-Guervilly, Fanny, additional, Kaplanski, Gilles, additional, Harlé, Jean-Robert, additional, Weiller, Pierre-Jean, additional, Berbis, Philippe, additional, Braunstein, David, additional, Jouve, Elisabeth, additional, Lesavre, Nathalie, additional, Couranjou, Françoise, additional, Dignat-George, Françoise, additional, Sabatier, Florence, additional, Paul, Pascale, additional, and Granel, Brigitte, additional

Published
2017
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33. High prevalence of laminopathies among patients with metabolic syndrome

Author

Roll, Patrice, Perrin, Sophie, Cremer, Jonathan, Faucher, Olivia, Reynes, Jacques, Dellamonica, Pierre, Micallef, Joëlle, Solas, Caroline, Lacarelle, Bruno, Stretti, Charlotte, Kaspi, Elise, Robaglia-Schlupp, Andrée, Tamalet, Corine Nicolino-Brunet Catherine, Lévy, Nicolas, Poizot-Martin, Isabelle, Cau, Pierre, Dutour, Anne, Gaborit, Bénédicte, Courrier, Sébastien, Alessi, Marie-Christine, Tregouet, David-Alexandre, Angelis, Fabien, Lesavre, Nathalie, BADENS, Catherine, Morange, Pierre-Emmanuel, Aix Marseille Université (AMU), Assistance Publique - Hôpitaux de Marseille (APHM), Laboratoire de Biologie Cellulaire [Hôpital de la Timone - APHM], Assistance Publique - Hôpitaux de Marseille (APHM)- Hôpital de la Timone [CHU - APHM] (TIMONE), Marseille medical genetics - Centre de génétique médicale de Marseille (MMG), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Aix Marseille Université (AMU), Département de génétique médicale [Hôpital de la Timone - APHM], and Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)- Hôpital de la Timone [CHU - APHM] (TIMONE)-Institut National de la Santé et de la Recherche Médicale (INSERM)

Subjects
Adult, Male, congenital, hereditary, and neonatal diseases and abnormalities, Dunnigan familial partial lipodystrophy, [SDV]Life Sciences [q-bio], Mutation, Missense, 030204 cardiovascular system & hematology, Biology, medicine.disease_cause, LMNA, Cohort Studies, 03 medical and health sciences, 0302 clinical medicine, Genetics, medicine, Missense mutation, Humans, Molecular Biology, Genetics (clinical), ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, Metabolic Syndrome, 0303 health sciences, Mutation, integumentary system, Membrane Proteins, Metalloendopeptidases, General Medicine, Middle Aged, medicine.disease, Familial partial lipodystrophy, Lamin Type A, Phenotype, 3. Good health, Cell nucleus, medicine.anatomical_structure, embryonic structures, Cancer research, Female, Lamin
Abstract

Constitutional laminopathies, such as the Dunnigan familial partial lipodystrophy, are severe diseases caused by mutations in A-type lamins and share several features with metabolic syndrome (MS). In this study, we hypothesized that MS may be, in some cases, a mild form of laminopathies and use the abnormal cell nucleus phenotype observed in these diseases as a primary screening test in patients suffering from common MS. Nuclear shape and lamin A nucleoplasmic distribution abnormalities were systematically searched in lymphoblastoid cells of 87 consecutive patients with MS. In parallel, five genes encoding either the A-type lamins or the enzymes of the lamin A maturation pathway were systematically sequenced (LMNA, ZMPSTE24, ICMT, FNTA and FNTB). We identified 10 MS patients presenting abnormal nuclear shape and disturbed lamin A/C nuclear distribution. These patients were not clinically different from those without nuclear abnormalities except that they were younger, and had higher triglyceridemia and SGPT levels. Three of them carry a heterozygous mutation in LMNA or in ZMPSTE24, a gene encoding one of the lamin A processing enzymes. All three mutations are novel missense mutations predicted to be damaging. Both lymphoblastoid cells and skin fibroblasts from the patient carrying the mutation in ZMPSTE24, showed accumulation of lamin A precursor, indicating an alteration of the lamin A processing, confirmed by functional study. Together, these results show for the first time, that a significant proportion of MS patients exhibits laminopathies and suggest that systematic investigation of lamin A and its partners should be performed at the diagnosis of this syndrome.

Published
2011

35. Sa1360 Canal Anal Pressure Profile At Rest and During Squeezing in Controls and Patients With Fecal Incontinence: A Study Using 3D Ano-Rectal High Resolution Manometry (3D ARHRM)

Author

Roman, Sabine, primary, Prieur, Fabrice, additional, Ropert, Alain, additional, Bouvier, Michel, additional, Damon, Henri, additional, Brochard, Charlene, additional, Vitton, Véronique, additional, Marjoux, Sophie, additional, Lesavre, Nathalie, additional, Siproudhis, Laurent, additional, and Mion, Francois, additional

Published
2015
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36. Increased serum levels of fractalkine and mobilisation of CD34+CD45- endothelial progenitor cells in systemic sclerosis.

Author

Benyamine, Audrey, Magalon, Jérémy, Cointe, Sylvie, Lacroix, Romaric, Arnaud, Laurent, Bardin, Nathalie, Rossi, Pascal, Francès, Yves, Bernard-Guervilly, Fanny, Kaplanski, Gilles, Harlé, Jean-Robert, Weiller, Pierre-Jean, Berbis, Philippe, Braunstein, David, Jouve, Elisabeth, Lesavre, Nathalie, Couranjou, Françoise, Dignat-George, Françoise, Sabatier, Florence, and Paul, Pascale

Published
2017
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37. Increased serum levels of fractalkine and mobilisation of CD34+CD45−endothelial progenitor cells in systemic sclerosis

Author

Benyamine, Audrey, Magalon, Jérémy, Cointe, Sylvie, Lacroix, Romaric, Arnaud, Laurent, Bardin, Nathalie, Rossi, Pascal, Francès, Yves, Bernard-Guervilly, Fanny, Kaplanski, Gilles, Harlé, Jean-Robert, Weiller, Pierre-Jean, Berbis, Philippe, Braunstein, David, Jouve, Elisabeth, Lesavre, Nathalie, Couranjou, Françoise, Dignat-George, Françoise, Sabatier, Florence, Paul, Pascale, and Granel, Brigitte

Abstract

The disruption of endothelial homeostasis is a major determinant in the pathogenesis of systemic sclerosis (SSc) and is reflected by soluble and cellular markers of activation, injury and repair. We aimed to provide a combined assessment of endothelial markers to delineate specific profiles associated with SSc disease and its severity. We conducted an observational, single-centre study comprising 45 patients with SSc and 41 healthy control subjects. Flow cytometry was used to quantify circulating endothelial microparticles (EMPs) and CD34+progenitor cell subsets. Colony-forming unit-endothelial cells (CFU-ECs) were counted by culture assay. Circulating endothelial cells were enumerated using anti-CD146-based immunomagnetic separation. Blood levels of endothelin-1, vascular endothelial growth factor (VEGF) and soluble fractalkine (s-Fractalkine) were evaluated by enzyme-linked immunosorbent assay. Disease-associated markers were identified using univariate, correlation and multivariate analyses. Enhanced numbers of EMPs, CFU-ECs and non-haematopoietic CD34+CD45−endothelial progenitor cells (EPCs) were observed in patients with SSc. Patients with SSc also displayed higher serum levels of VEGF, endothelin-1 and s-Fractalkine. s-Fractalkine levels positively correlated with CD34+CD45−EPC numbers. EMPs, s-Fractalkine and endothelin-1 were independent factors associated with SSc. Patients with high CD34+CD45−EPC numbers had lower forced vital capacity values. Elevated s-Fractalkine levels were associated with disease severity, a higher frequency of pulmonary fibrosis and altered carbon monoxide diffusion. This study identifies the mobilisation of CD34+CD45−EPCs and high levels of s-Fractalkine as specific features of SSc-associated vascular activation and disease severity. This signature may provide novel insights linking endothelial inflammation and defective repair processes in the pathogenesis of SSc.

Published
2017
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38. Endoscopic ultrasound-guided fine-needle aspiration biopsy coupled with KRAS mutation assay to distinguish pancreatic cancer from pseudotumoral chronic pancreatitis.

Author

Bournet, Barbara, Souque, Anny, Senesse, Pierre, Assénat, Eric, Barthet, Marc, Lesavre, Nathalie, Aubert, Ariane, O'toole, Dermot, Hammel, Pascal, Levy, Philippe, Ruszniewski, Philippe, Bouisson, Michèle, Escourrou, Jean, Cordelier, Pierre, Buscail, Louis, Bournet, Barbara, Souque, Anny, Senesse, Pierre, Assénat, Eric, Barthet, Marc, Lesavre, Nathalie, Aubert, Ariane, O'toole, Dermot, Hammel, Pascal, Levy, Philippe, Ruszniewski, Philippe, Bouisson, Michèle, Escourrou, Jean, Cordelier, Pierre, and Buscail, Louis

Abstract

BACKGROUND AND STUDY AIMS: Differential diagnosis between pancreatic adenocarcinoma (PADC) and pseudotumoral forms of chronic pancreatitis remains difficult. Mutation of KRAS oncogene is present in 75% to 95% of PADC. This study aimed to evaluate whether the combined analysis of KRAS mutation with cytopathological findings from endoscopic ultrasound-guided fine-needle aspiration biopsy (EUS-FNAB) might improve discrimination between PADC and chronic pancreatitis. PATIENTS AND METHODS: This prospective multicenter study included 178 patients with solid pancreatic masses (men 104, women 74; mean age 64.5 years). Cytopathological examination and KRAS mutation analysis (codon-12 and codon-13, restriction fragment length polymorphism [RFLP] and direct sequencing) were performed on EUS-FNAB material. Final diagnoses were obtained on EUS-FNAB analysis and/or a second biopsy and/or clinical follow-up and/or surgery: PADC, n = 129; chronic pancreatitis, n = 27; other pancreatic neoplasms, n = 16; and benign lesions, n = 6. RESULTS: KRAS status analysis was successful in all EUS-FNAB samples. Codon-12 KRAS point mutation was found in 66% of PADC samples. No case of chronic pancreatitis displayed KRAS mutation. Sensitivity, specificity, positive and negative predictive values, and overall accuracy of cytopathology alone for diagnosis of PADC versus chronic pancreatitis were 83%, 100%, 100%, 56% and 86%, respectively. When KRAS mutation analysis was combined with cytopathology, these values reached 88%, 100%, 100%, 63% and 90% respectively. CONCLUSION: Although the value of KRAS analysis in addition to EUS-FNAB is limited for distinguishing pancreatic mass lesions, when chronic pancreatitis presented as a pseudotumor a negative finding (wild-type KRAS), was useful in strongly suggesting a benign lesion.

Published
2009

40. Hypoxia-inducible factor (HIF1α) gene expression in human shock states

Author

Textoris, Julien, primary, Beaufils, Nathalie, additional, Quintana, Gabrielle, additional, Ben Lassoued, Amin, additional, Zieleskiewicz, Laurent, additional, Wiramus, Sandrine, additional, Blasco, Valéry, additional, Lesavre, Nathalie, additional, Martin, Claude, additional, Gabert, Jean, additional, and Leone, Marc, additional

Published
2012
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41. Level of Adenosine Diphosphate Receptor P2Y12 Blockade During Percutaneous Coronary Intervention Predicts the Extent of Endothelial Injury, Assessed by Circulating Endothelial Cell Measurement

Author

Bonello, Laurent, primary, Harhouri, Karim, additional, Sabatier, Florence, additional, Camoin-Jau, Laurence, additional, Arnaud, Laurent, additional, Baumstarck-Barrau, Karine, additional, Ait-Mokhtar, Omar, additional, Roubille, François, additional, Piot, Christophe, additional, Lesavre, Nathalie, additional, Paganelli, Franck, additional, and Dignat-George, Françoise, additional

Published
2010
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42. Association Between a CTGF Gene Polymorphism and Systemic Sclerosis in a French Population

Author

GRANEL, BRIGITTE, primary, ARGIRO, LAURENT, additional, HACHULLA, ERIC, additional, FAJARDY, ISABELLE, additional, WEILLER, PIERRE-JEAN, additional, DURAND, JEAN-MARC, additional, FRANCES, YVES, additional, DOMBEY, ANNE-MARIE, additional, MARQUET, SANDRINE, additional, LESAVRE, NATHALIE, additional, DISDIER, PATRICK, additional, BERNARD, FANNY, additional, HATRON, PIERRE-YVES, additional, and CHEVILLARD, CHRISTOPHE, additional

Published
2009
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44. High Atopobium vaginae and Gardnerella vaginalis Vaginal Loads Are Associated With Preterm Birth.

Author

Bretelle, Florence, Rozenberg, Patrick, Pascal, Alain, Favre, Romain, Bohec, Caroline, Loundou, Anderson, Senat, Marie-Victoire, Aissi, Germain, Lesavre, Nathalie, Brunet, Julie, Heckenroth, Hélène, Luton, Dominique, Raoult, Didier, and Fenollar, Florence

Subjects
BACTERIAL vaginitis treatment, PREGNANCY complications, BACTERIAL vaginitis diagnosis, RISK factors in premature labor, GARDNERELLA, MOLECULAR biology, MISCARRIAGE
Abstract

Molecular biology can diagnose and quantify vaginal flora anomalies. High vaginal loads of Atopobium vaginae and Gardnerella vaginalis are associated with preterm birth and shorter pregnancies. A. vaginae may be a useful target in preventing preterm birth.Background. Bacterial vaginosis is a risk factor for preterm birth. The various conventional methods for its diagnosis are laborious and not easily reproducible. Molecular quantification methods have been reported recently, but the specific risk factors they might identify remain unclear.Methods. A prospective multicenter national study included pregnant women at risk of preterm birth. A quantitative molecular tool using a specific real-time polymerase chain reaction assay and serial dilutions of a plasmid suspension quantified Atopobium vaginae, Gardnerella vaginalis, lactobacilli, Mycoplasma hominis, and the human albumin gene (for quality control).Results. In 813 pregnancies, high vaginal loads of either or both of A. vaginae and G. vaginalis were associated with preterm birth (hazard ratio [HR], 3.9; 95% confidence interval {CI}, 1.1–14.1; P = .031). A high vaginal load of A. vaginae was significantly associated with shortened time to delivery and therefore pregnancy length. These times were, respectively, 152.2 and 188.2 days (HR, 5.6; 95% CI, 1.5–21.3; P < .001) before 22 weeks, 149.0 and 183.2 days (HR, 2.8; 95% CI, 1.1–8.2; P = .048) before 28 weeks, and 132.6 and 170.4 days (HR, 2.2; 95% CI, 1.1–4.6; P = .033) before 32 weeks. After multivariate analysis, A. vaginae levels ≥108 copies/mL remained significantly associated with delivery before 22 weeks of gestation (adjusted HR, 4.7; 95% CI, .2–17.6; P = .014).Conclusions. High vaginal loads of A. vaginae and G. vaginalis are associated with late miscarriage and prematurity in high-risk pregnancies. A high vaginal load of A. vaginae (DNA level ≥108 copies/mL) identifies a population at high risk of preterm birth. Further studies that both screen for and then treat A. vaginae are needed.Clinical Trials Registration. NCT00484653. [ABSTRACT FROM PUBLISHER]

Published
2015

46. Targeting Tissular Immune Response Improves Diagnostic Performance of Anti-Saccharomyces cerevisiae Antibodies (ASCA) in Crohn’s Disease.

Author

Bertin, Daniel, Grimaud, Jean-Charles, Lesavre, Nathalie, Benelmouloud, Chahine, Desjeux, Ariadne, Garcia, Stéphane, and Desplat-Jégo, Sophie

Subjects
CROHN'S disease diagnosis, GENE targeting, IMMUNE response, IMMUNOGLOBULINS, SACCHAROMYCES cerevisiae, PORINS (Proteins), MEMBRANE proteins, ESCHERICHIA coli
Abstract

Antibodies against Saccharomyces cerevisiae (ASCA) and Escherichia coli outer membrane porin C (anti-OmpC) are known to be detectable in the serum of patients with Crohn’s disease (CD) but display a very poor sensitivity for the disease especially in forms with isolated colonic involvement. In this study we aimed at evaluating performances of these markers in supernatant of cultured colonic biopsies. Patients with colonic CD (n =  67), ulcerative colitis (UC) (n = 35) and control individuals (n = 37) were prospectively recruited for colonoscopy pinch biopsies and blood sampling. Serum and supernatant of culture tissues were analyzed for ASCA and anti-OmpC. Direct immunofluorescence was also performed on colonic tissues for total IgA detection. We detected for the first time ASCA IgA/IgG and anti-OmpC IgA in cultured colonic tissue supernatants. For both markers, sensitivities for diagnosing CD were better in supernatants (ASCA: 53.7%, anti-OmpC: 28.4%) than in serum (ASCA: 31.3%, anti-OmpC: 22.4%). Combination of results from a panel of these tests gave the greatest sensitivity ever described for CD diagnosis in colonic forms (70.2%). In this study, we described, for the first time, ASCA in supernatant of colonic tissue cultures. This assaying approach in CD diagnosis should be taken into consideration in the future especially in CD forms with isolated colonic involvement. [ABSTRACT FROM AUTHOR]

Published
2013
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47. p8 inhibits the growth of human pancreatic cancer cells and its expression is induced through pathways involved in growth inhibition and repressed by factors promoting cell growth.

Author

Malicet, Cédric, Lesavre, Nathalie, Vasseur, Sophie, and Iovanna, Juan L.

Subjects
*PROTEINS, *PANCREATIC cancer, *CANCER cell growth, *GENE expression, *GENE silencing, *CANCER genetics
Abstract

Background: p8 is a stress-induced protein with multiple functions and biochemically related to the architectural factor HMG-I/Y. We analyzed the expression and function of p8 in pancreatic cancer-derived cells. Methods: Expression of p8 was silenced in the human pancreatic cancer cell lines Panc-1 and BxPc- 3 by infection with a retrovirus expressing p8 RNA in the antisense orientation. Cell growth was measured in control and p8-silenced cells. Influence on p8 expression of the induction of intracellular pathways promoting cellular growth or growth arrest was monitored. Results: p8-silenced cells grew more rapidly than control cells transfected with the empty retrovirus. Activation of the Ras→Raf→MEK→ERK and JNK intracellular pathways down-regulated p8 expression. In addition, the MEK1/2 inhibitor U0126 and the JNK inhibitor SP600125 up-regulates expression of p8. Conversely, p38 or TGFβ-1 induced p8 expression whereas the specific p38 inhibitor SB203580 down-regulated p8 expression. Finally, TGFβ-1 induction was in part mediated through p38. Conclusions: p8 inhibits the growth of human pancreatic cancer cells. p8 expression is induced through pathways involved in growth inhibition and repressed by factors that promote cell growth. These results suggest that p8 belongs to a pathway regulating the growth of pancreatic cancer cells. [ABSTRACT FROM AUTHOR]

Published
2003

48. Association Between a CTGF Gene Polymorphism and Systemic Sclerosis in a French Population

Author

GRANEL, BRIGITTE, ARGIRO, LAURENT, HACHULLA, ERIC, FAJARDY, ISABELLE, WEILLER, PIERRE-JEAN, DURAND, JEAN-MARC, FRANCES, YVES, DOMBEY, ANNE-MARIE, MARQUET, SANDRINE, LESAVRE, NATHALIE, DISDIER, PATRICK, BERNARD, FANNY, HATRON, PIERRE-YVES, and CHEVILLARD, CHRISTOPHE

Abstract

OBJECTIVE: Systemic sclerosis (SSc) is a life-threatening autoimmune disease characterized by chronic fibrosis of the skin and internal organs. Connective tissue growth factor (CTGF) is believed to be a primary mediator of chronic fibrosis. We assessed the possible association between 7 single-nucleotide polymorphisms (SNP) in the CTGF gene and scleroderma in a French population (registration number 2006/0182). METHODS: We conducted a case-control study with 241 scleroderma patients and 269 controls. Seven SNP were genotyped using the TaqMan system. Univariate and multivariate analyses were performed. In silico electrophoretic mobility shift assay (EMSA), and reverse transcriptase polymerase chain reaction analyses were done to assess the effect of the SNP on CTGF gene expression. RESULTS: The frequency of the rs9399005TT genotype was significantly lower in SSc patients than in controls. This association remained significant after adjustment for gender. An association was detected between the rs9399005 and the diffuse and limited cutaneous forms. Multivariate analysis between SSc patients and controls taking into account all 7 SNP and sex revealed that only sex and the rs9399005 SNP were associated with disease. DNA analysis by EMSA indicated that the T allele bound nuclear factors that were also bound by the C allele. The binding affinity was higher for the T allele. Analysis of the human database and experiments with human hepatocyte cell line indicated the existence of an alternative transcript containing the rs9399005 polymorphism in its 3’UTR region. In silico analysis indicated that this polymorphism may alter the structure of CTGF messenger RNA. CONCLUSION: These findings suggest that CTGF gene polymorphisms may contribute to susceptibility to scleroderma.

Published
2010

50. ⁎⁎Invited to participate in the Poster Session of the ASGE meeting.3392 Refeeding patients after endoscopic sphincterotomy: waiting or not waiting ?

Author

Bellon, Philippe, Lesavre, Nathalie, Barthet, Marc, Barriere, Nicolas, Desjeux, Ariadne, Grimaud, Jean-Charles, and Salducci, Jacques

Abstract

Background: After endoscopic sphincterotomy, refeeding is usually delayed for 1 or 2 days after ERCP. The aim of this study was to investigate the necessity of delayed refeeding in patients undergoing endoscopic biliary or pancreatic sphincterotomy (EBPS). Methods: From November 1998 to September 1999, 60 patients with previous normal alimentation were prospectively randomised after EBPS in two groups. Patients belonging to the goup A were allowed for early refeeding (6 hours delayed) whereas patients belonging to the group B were asked to wait the following day before refeeding. Results: The two groups were comparable for age (67 vs 66 years old), levels of serum amylase and lipase before ERCP, indication of ERCP (pancreatic cancer, common bile duct stone, cholangitis, sphincter of Oddi dysfunction, acute or chronic pancreatitis). No difference could be shown concerning the precut papillotomy rate, the biliary or pancreatic stenting rate, the rate of removed choledocal stones, the need for endoscopic hemostasis. Two patients (one in each group) were excluded few hours after randomisation for severe acute pancreatitis. 16 (27.6 %) out of the 58 remaining patients suffered from abdominal pain after ERCP. 13 of them healed with paracetamol infusion and 3 needed morphinic infusion. The paracetamol consumption in group A was significantly lower than in group B (2 vs 11, p = 0.007). In group A, no patient suffered from nausea or vomiting and only one experienced pain during refeeding, requiring paracetamol administration. No statistical difference was shown regarding to the serum level of amylase and lipase after ERCP. The duration of hospital stay was decreased in patients with early refeeding without reaching statistical significance (group A: 2.9 days and group B: 4.1 days, p = 0.15). Conclusion: Early refeeding is well tolerated and decreases significantly the need for paracetamol after endoscopic sphincterotomy. A trend toward a decrease of the duration of the hospital stay was observed in this study.

Published
2000
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