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Summary mRNA is thought to predominantly reside in the cytoplasm, where it is translated and eventually degraded. Although nuclear retention of mRNA has a regulatory potential, it is considered extremely rare in mammals. Here, to explore the extent of mRNA retention in metabolic tissues, we combine deep sequencing of nuclear and cytoplasmic RNA fractions with single-molecule transcript imaging in mouse beta cells, liver, and gut. We identify a wide range of protein-coding genes for which the levels of spliced polyadenylated mRNA are higher in the nucleus than in the cytoplasm. These include genes such as the transcription factor ChREBP, Nlrp6, Glucokinase, and Glucagon receptor. We demonstrate that nuclear retention of mRNA can efficiently buffer cytoplasmic transcript levels from noise that emanates from transcriptional bursts. Our study challenges the view that transcripts predominantly reside in the cytoplasm and reveals a role of the nucleus in dampening gene expression noise. [ABSTRACT FROM AUTHOR]

Extensive measurements of the X-ray background (XRB) yield a reasonably reliable characterization of its basic properties. Having resolved most of the cosmic XRB into discrete sources, the levels and spectral shapes of its main components can be used to probe both the source populations and also alternative cosmological and large-scale structure models. Recent observations of clusters seem to provide evidence that clusters formed earlier and are more abundant than predicted in the standard Λ cold dark matter (ΛCDM) model. This motivates interest in alternative models that predict enhanced power on cluster scales. We calculate predicted levels and spectra of the superposed emission from groups and clusters of galaxies in ΛCDM and in two viable alternative non-Gaussian and early dark energy models. The predicted levels of the contribution of clusters to the XRB in the non-Gaussian models exceed the measured level at low energies and levels of the residual XRB in the 2–8 keV band; these particular models are essentially ruled out. Our work demonstrates the diagnostic value of the integrated X-ray emission from clusters, by considering also its dependences on different metallicities, gas and temperature profiles, Galactic absorption, merger scenarios and on a non-thermal pressure component. We also show that the XRB can be used for an upper limit for the concentration parameter value. [ABSTRACT FROM AUTHOR]

We carry out a comprehensive joint analysis of high-quality HST/ACS and Chandra measurements of A1689, from which we derive mass, temperature, X-ray emission and abundance profiles. The X-ray emission is smooth and symmetric, and the lensing mass is centrally concentrated indicating a relaxed cluster. Assuming hydrostatic equilibrium we deduce a 3D mass profile that agrees simultaneously with both the lensing and X-ray measurements. However, the projected temperature profile predicted with this 3D mass profile exceeds the observed temperature by ∼30 per cent at all radii, a level of discrepancy comparable to the level found for other relaxed clusters. This result may support recent suggestions from hydrodynamical simulations that denser, more X-ray luminous small-scale structure can bias observed temperature measurements downward at about the same (∼30 per cent) level. We determine the gas entropy at (where rvir is the virial radius) to be ∼800 keV cm2, as expected for a high-temperature cluster, but its profile at has a power-law form with index ∼0.8, considerably shallower than the ∼1.1 index advocated by theoretical studies and simulations. Moreover, if a constant entropy ‘floor’ exists at all, then it is within a small region in the inner core, , in accord with previous theoretical studies of massive clusters. [ABSTRACT FROM AUTHOR]