1. Elicitation strategies for the improvement of essential oil content in Rhododendron tomentosum (Ledum palustre) bioreactor-grown microshoots.
- Author
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Jesionek, Anna, Kokotkiewicz, Adam, Krolicka, Aleksandra, Zabiegala, Bozena, and Luczkiewicz, Maria
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ELICITORS (Botany) , *ESSENTIAL oils , *LEDUM groenlandicum , *BIOREACTORS , *PLANT shoots , *TERPENES - Abstract
Graphical abstract Highlights • The effect of abiotic and biotic elicitors on R. tomentosum essential oil was tested. • The aphid extract was used as a new elicitor of insect origin in plant biotechnology. • The elicitors most suitable for improving the production of terpenes were selected. • Addition of nickel salts, the aphid extract and P. carotovorum gave promising results. • The RITA® bioreactor was suitable for elicitation studies in plant in vitro cultures. Abstract Rhododendron tomentosum Harmaja, previously Ledum palustre L., is a fragrant plant growing in swampy areas, which is characterized by well-known repellent properties and the anti-arthritic potential. In the present study, R. tomentosum microshoots cultivated in RITA® bioreactor, providing the alternative, environment-independent source of the valuable essential oil, were treated for 7 days with copper and nickel salts, methyl jasmonate, chitosan, ergosterol, lysates of: Candida albicans , Escherichia coli , Enterobacter sakazaki, Pectobacterium carotovorum and Dickeya dadantii , to stimulate the production of secondary metabolites. Moreover, for the first time, the aphid ethanol extract was tested as an elicitor of insect origin. Suspensions of all pathogens added to in vitro culture reduced significantly the biomass growth parameters. The application of the aphid extract and lysate of P. carotovorum increased the volatile fraction content by 14%, while Ni2+ and ergosterol by 8%. The influence of the studied elicitors on the chemical composition of R. tomentosum essential oils was presented and discussed thoroughly, taking into account a percentage of 22 main terpenoid compounds, determined by GC/MS (i.a. p -cymene, geranyl acetate, δ -cadinene, shyobunone, methyl everninate, ledene oxide (II) and alloaromadendrene). The biosynthesis of monoterpenes was primarily proven to be affected by the elicitation. The conducted experiments suggest that R. tomentosum is rather resistant to elicitation strategies, both abiotic and biotic as well as methyl jasmonate. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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