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In the COVID-19 pandemic, delivering learning content to students via the use of technologies has become mainstream. Among various technology-supported learning modes, game-based language learning (GBLL) has been considered an effective approach to engaging learners in joyful and interactive contexts. This study aims to provide an overview of GBLL using bibliometric mapping analysis and coding analysis. This systematic review provides a scoping overview of empirical evidence on the use and impacts of games in language learning from 1989 to 2020. Based on a set of criteria retrieved from the Web of Science, 101 articles were analyzed. The coding analysis were three aspects to identify the research issues, performance issues, and interaction issues. Moreover, in a comprehensive review of the research on GBLL, insights are provided for educators and future research. The findings differing from those of previous reviews can serve as a reference for researchers on GBLL-related studies.

A new method was used to study the performance of pile across cave. This paper investigated the vertical bearing characteristics of piles cross caves using centrifugal model tests and a theoretical model of sensitivity. Twelve pile scenarios were selected, the first was a conventional pile, 24 cm long and 2.5 cm in diameter, with no karst cave as a control. In the other eleven scenarios the piles passed through karst caves of four different heights, of four different spans, and three different numbers of caves. The results reveal that increasing the height, span, and number of caves all are negative for vertical ultimate bearing capacity of piles. The axial force and unit shaft resistance of piles are great different. According to the ratios of the tip and shaft resistance, caves change the type of piles. The sensitivity of vertical ultimate bearing capacity to these factors from high to low is height, number, and span of caves. Importantly, the bearing characteristics of piles decrease faster once the height of the prototype karst cave is higher than 9 m, but decreases slowly when the cave’s span is greater than 9 m × 9 m.

OBJECTIVES: To evaluate myocardial injury in neonates born to pregnant women with pregnancy complicated by severe preeclampsia by myocardial work indices. METHODS: A prospective cohort study was performed on 25 preterm infants born to the pregnant women with severe preeclampsia from June 2020 to April 2021 (severe preeclampsia group), and 25 preterm infants born to the pregnant women without severe complications in pregnancy were enrolled as the control group. Echocardiography was performed within 24 hours and at 48-72 hours and 14-28 days after birth to measure conventional parameters. Two-dimensional speckle-tracking echocardiography was performed to construct a noninvasive left ventricular pressure-strain loop based on two-dimensional myocardial strain and left ventricular systolic pressure noninvasively measured, so as to calculate myocardial work indices. RESULTS: Compared with the control group, the severe preeclampsia group had significant reductions in left ventricular global work index and global constructive work within 24 hours after birth (P

Taking Puqian bridge as the prototype, a 1:30-scale pile-soil-fault interaction model was established. Through the shaking table test, the difference of dynamic response of pile foundation on both sides of fault under 0.15~0.60g ground motion intensity was studied. The pile acceleration, pile top relative displacement, and pile bending moment on both sides of the fault are compared respectively. Research results showed that under the action of a strong earthquake, the pile foundation on the hanging wall was greatly affected by ground motion, and “the hanging wall effect” was significant. As the ground motion intensity increased, the “hanging wall effect” of the pile foundation was more obvious. Combined with the fundamental frequency response and the test phenomenon, when ground motions intensity was strong, cracks appeared near the joint of pile top and platform, soil interface, and bedrock surface. When building a bridge pile foundation near the fault, the seismic design of the pile foundation on the hanging wall of the fault is mainly considered.

Benzo[a]pyrene (BaP), a common environmental pollutant, can modulate the immune-associated signal pathway NF-κB, which is one of the critical signal pathways involved in various immune responses. BaP exposure usually generates reactive oxygen species (ROS), but whether ROS are predominantly involved in the modulation mechanism of the NF-κB pathway has not been clearly understood. In this study, an in vivo examination of Oryzias melastigma demonstrated that BaP exposure led to a down-regulation of the NF-κB pathway and increased levels of ROS. Conversely, in vitro results using the medaka liver cell line DIT-29 and a widely applied H2O2 method showed the opposite: up-regulation of the NF-κB pathway. However, the down-regulation of NF-κB upon BaP exposure in vitro was inhibited by the addition of a ROS inhibitor, indicating ROS are involved in the modulation of NF-κB. The discrepancy between in vivo and in vitro results of ROS impacts on NF-κB activation might be related to the concentration and persistence of ROS. Using a modified luminol detection system, BaP was found to generate sustained physiological concentrations of ROS for 24 h, while an H2O2 bolus generated ROS for less than 30 min. Furthermore, a steady-state sub-micromolar H2O2 system (H2O2ss) was developed in parallel as a positive control of ROS, by which H2O2 could be maintained for 24 h. Comparative evaluation using H2O2, H2O2ss and BaP exposures on the medaka cell line with pGL4.32 demonstrated that the persistent physiological concentrations of ROS generated upon BaP exposure or treatment with H2O2ss inhibited the NF-κB pathway, but direct H2O2 exposure had the opposite effect. Moreover, a western-blot assay and EMSA detection further confirmed the modulation of the NF-κB pathway in DIT-29. Taken together, this study shows that BaP exposure inhibits the NF-κB pathway by generating sustained physiological concentrations of ROS.

Defensive behavior, a group of responses that evolved due to threatening stimuli, is crucial for animal survival in the natural environment. For defensive measures to be timely and successful, a high arousal state and immediate sleep-to-wakefulness transition are required. Recently, the glutamatergic basal forebrain (BF) has been implicated in sleep-wake regulation; however, the associated physiological functions and underlying neural circuits remain unknown. Here, using in vivo fiber photometry, we found that BF glutamatergic neuron is activated by various threatening stimuli, including predator odor, looming threat, sound, and tail suspension. Optogenetic activation of BF glutamatergic neurons induced a series of context-dependent defensive behaviors in mice, including escape, fleeing, avoidance, and hiding. Similar to the effects of activated BF glutamatergic cell body, photoactivation of BF glutamatergic terminals in the ventral tegmental area (VTA) strongly drove defensive behaviors in mice. Using synchronous electroencephalogram (EEG)/electromyogram (EMG) recording, we showed that photoactivation of the glutamatergic BF-VTA pathway produced an immediate transition from sleep to wakefulness and significantly increased wakefulness. Collectively, our results clearly demonstrated that the glutamatergic BF is a key neural substrate involved in wakefulness and defensive behaviors, and encodes these behaviors through glutamatergic BF-VTA pathway. Overexcitation of the glutamatergic BF-VTA pathway may be implicated in clinical psychiatric diseases characterized by exaggerated defensive responses, such as autism spectrum disorders.

Background To systematically assess the quality of reports of clinical trials of stem cell for heart diseases published in Chinese. Methods The quality of reports was assessed according to the CONSORT statement and the Jadad score. The association between the CONSORT scores and the reported therapeutic effects was evaluated. Results A total of 36 randomized clinical trials were identified, and 1552 patients were included. The mean CONSORT score was 7.06 (SD = 2.99). The proportion of reports with a Jadad score of 3 was 8.33%. The improvement of left ventricular function, myocardial perfusion area, left ventricular diastolic diameter, and cardiac output decreased with the increase in the CONSORT score. Conclusions The percentages of high-quality reports published in Chinese on stem cell therapy for heart diseases are low. Although stem cell transplantation seems promising for heart diseases, high-quality studies are needed to verify the conclusions．.

The glutamatergic lateral hypothalamus (LH) has been implicated in a variety of behaviors, such as evasion and feeding, while its role in defensive behaviors and relevant neurocircuits remains unclear. Here, we demonstrated that the glutamatergic LH is a critical structure regulating defensive behaviors. Trimethylthiazole (TMT), the odor of mice predator, significantly increased c-Fos expression in the LH. Using fiber photometry technology, we found that TMT exposure increased the activity of LH glutamatergic neurons. Selective activation of LH glutamatergic neurons with optogenetics and chemogenetics promoted a series of defense-related behaviors, including fleeing, avoidance, and hiding, while selective inhibition of LH glutamatergic neurons suppressed the avoidance provoked by TMT. Activation of both the glutamatergic LH terminals in the hypothalamic paraventricular nucleus (PVN) and the glutamatergic projection from the basolateral amygdala (BLA) to the LH elicited defensive behaviors. Finally, by combining the viral-mediated retrograde tracing with anterograde activation, we found that PVN-projecting glutamatergic neurons in the LH were activated by BLA glutamatergic inputs. Taken together, our results illustrate that the glutamatergic LH is a pivotal relay of defensive behaviors and possibly promotes these behaviors through the BLA→LH→PVN pathway.

Pubertal molt is critical for the reproductive process of mud crab Scylla paramamosain, during which female crabs molt and mate with males, and subsequently, ovarian development is initiated. Since fasting occurs during pubertal molt, the mechanism underlying how the internal energy reserves support crabs' survival is unclear. In this study, the hepatopancreas and ovaries were collected for metabolomics analyses. The results show 11 carbohydrate and 4 lipid metabolic pathways were remarkably modulated in hepatopancreas, suggesting carbohydrates and lipids may be the main energy reserves in hepatopancreas postmolt. Further analysis showed that the levels of glucose, glucose 6-phosphate, glucose 1-phosphate, and fructose 6-phosphate were increased, indicating glycolysis was upregulated for energy supply. Meanwhile, 65 lipids were significantly changed, including 16 glycerophospholipids and 33 fatty acids, suggesting lipids might be another main energy supplier in the hepatopancreas. In the ovary, 7 carbohydrate and 1 lipid metabolic pathways were obviously affected, and 12 carbohydrates and 23 lipids were also significantly changed postmolt. Differently from the changes in the hepatopancreas, the glucose level was significantly decreased in the ovary, whereas the levels of eight glycerophospholipids and four unsaturated fatty acids were increased in the ovary but decreased in the hepatopancreas, suggesting lipids might be major energy reserves for ovarian development, and they were possibly exported from hepatopancreas. Moreover, the obvious changes in seven fatty acyl carnitines in the ovary indicate they were consumed via β-oxidation postmolt. Taken together, carbohydrates and fatty acyl carnitines were clearly the major energy reserves in pubertal molt. The hepatopancreas played a central role in the supply of energy reserves and nutrients. Numerous glycerophospholipids and unsaturated fatty acids may have accumulated in the ovary for the biosynthesis of egg yolk during ovarian development. This study suggests that proper increases in carbohydrate and lipid levels in crab feed ahead of pubertal molt may alleviate the effects of fasting and enhance successful reproduction.

Pubertal molt is a vital stage in the cultivation of mature female crabs in the aquacultural industry of Scylla paramamosain. Since fasting occurs during molting, which requires a large supply of energy, internal energy reserves are critical. However, the dynamics of energy supply during pubertal molt is not clear. This study focuses on the variations of carbohydrates and lipids in serum during the pubertal molt of S. paramamosain via a metabolomics approach. Eleven lipid or carbohydrate metabolic pathways were significantly influenced postmolt. A remarkable decrease in carbohydrates in serum suggested that free sugars were consumed for energy. A significant decrease in glucose and alpha-d-glucosamine 1-phosphate showed that chitin synthesis exhausted glycogen, resulting in insufficient glucose supply. An increase in l-carnitine and acetylcarnitine, and a significant decrease in 15 fatty acyls and 8 glycerophosphocholines in serum indicated that carnitine shuttle was stimulated, and β-oxidation was upregulated postmolt. In addition, astaxanthin, ponasterone A, and riboflavin in serum were significantly decreased postmolt. Eleven potential metabolite biomarkers were identified for pubertal molt. Taken together, carbohydrates and lipids were possibly major energy reserves in pubertal molt. This study suggests that an increase in carbohydrate and lipid levels in crab feed may alleviate the effects of fasting during molt and improve farm productivity in mature female crabs.

Benzo[a]pyrene (BaP), a widely existed polycyclic aromatic hydrocarbon pollutant in aquatic environment, has toxic effects on marine animals and their generations, but the intergenerational immunotoxic mechanism underlying has not been clearly understood. In the study, the offspring of marine medaka (oryzias melastigma) which were exposed to 0.5 μg L−1 BaP suffered from circadian rhythm oscillation disorders and severe DNA damage. Many clock-associated genes like per1 were significantly modulated in offspring, both per1 and p53 were significantly inhibited that altered the progression of cell cycle and inhibited DNA repair, which possibly resulted in the increased mortality of offspring. The hypermethylation of the per1 promotor and abnormal levels of N6-methyladenosine (m6A) suggested that the underlying mechanism was probably related to the epigenetic modification. Moreover, the offspring from paternal BaP exposure had more severe DNA damage and a higher degree of hypermethylation than those from maternal exposure. F1 larvae from BaP-exposed parents were more sensitive to BaP exposure, showing that the expression of immune and metabolism-related genes were significantly up-regulated. Taken together, the parental toxicity induced by BaP could be passed to F1 generation and the mechanism underlying was probably associated with a characteristic circadian rhythm disorder.

A new antimicrobial peptide named SCY2 with 65.08% identity in amino acid sequence to the known scygonadin (SCY1) was first characterized in Scylla paramamosain based on its cloned full-length cDNA and genomic DNA sequences. The SCY2 gene was dominantly expressed in the ejaculatory duct of male crabs and its mRNA transcripts were discerned mainly in the glandular epithelium of the inner wall and the secretion inside the ejaculatory duct. Although the SCY2 gene could not be induced with the challenge of the bacteria and fungi tested, its induction reached the highest level at the peak period of mating in mature male crabs either in June or November, suggesting its induction was likely related to seasonal reproduction changes. Moreover, it was interesting to note that, from analysis of its transcripts and protein, SCY2 was significantly expressed only in the ejaculatory duct of pre-copulatory males before mating, however it was clearly detected in the spermatheca of post-copulatory females after mating accompanied by the decreased level of SCY2 expression in the ejaculatory duct. These results suggested that the SCY2 was probably transferred from the male during mating action with the female for the purpose of protecting fertilization. The recombinant SCY2 was more active against the Gram-positive than the Gram-negative bacteria tested. It was further observed that the SCY2 transcripts were significantly increased with addition of exogenous progesterone in tissue cultures whereas the several other hormones tested had no any effect on SCY2 expression, indicating that there might be a relationship between the SCY2 expression and the induction of hormones in vivo. In summary, this study demonstrated that one role of SCY2 was likely to be involved in crab reproduction and it exerted its reproductive immune function through the mating action and the maintenance of inner sterility in the spermatheca of the female, thus leading to successful fertilization of S. paramamosain.

The obesity epidemic is a global problem and a great challenge for public health. Overconsumption of food, especially palatable food, is the leading cause of obesity. The precise neural circuits underlying food overconsumption remain unclear and require further characterization. In the present study, we showed that Ca2+ signals of GABAergic neurons within the ventral tegmental area (VTA) increased after the onset of food intake, especially high-fat or high-sugar chow. Optogenetic activation of VTA GABAergic neurons evoked immediate eating of palatable food and significantly increased palatable food intake in satiated mice. Photoinhibition of VTA GABAergic neurons suppressed palatable food intake. Surprisingly, photoactivation of VTA GABAergic neurons suppressed the intake of standard chow in fasted mice, but did not reduce the duration of eating of standard chow. Moreover, we found that photoactivation of these neurons drove a series of anxiety-like behaviors in the open field, elevated plus maze, and marble-burying test. Additionally, we found that VTA GABAergic neurons sent abundant projections to the lateral hypothalamus and photoactivation of GABAergic VTA terminals in the lateral hypothalamus induced overconsumption of palatable food, but not anxiety-like behaviors. Taken together, our results illustrate that GABAergic VTA neurons are a key node in the neural circuitry underlying anxiety-like behavior and over-feeding of palatable food, and that over-excitation of GABAergic VTA neurons may underlie clinical diseases related to anxiety and obesity.

Two marine antimicrobial peptides (AMPs), PC-hepc from large yellow croaker (Pseudosciaena crocea) and scygonadin from mud crab (Scylla serrata), are potently active against specific bacteria and thus they could be used as substitutes for antibiotics in aquaculture. However, how to utilize the AMPs feasibly for marine cultured animals has been so far confused. In our study, a 510 bp of the Scy-hepc sequence was cloned into pMDC85 expression vector, which was then electroporated into Chlorella sp., and thus a transgenic Chlorella, in which the Scy-hepc gene was effectively expressed, was developed. The Scy-hepc fusion protein was successfully expressed in Chlorella sp. and it showed obvious bactericidal activity. In addition, the in vivo efficacy of the transgenic Chlorella was evaluated using Sparus macrocephalus and the hybrid Epinephelus fuscoguttatus (♀) × Epinephelus lanceolatus (♂). Results showed that the survival rate of S. macrocephalus fed with transgenic Chlorella (80 ± 10% after 72 h) was significantly higher than that of fish fed with the same dosage of wild-type Chlorella (33.33 ± 11.55% after 72 h). Similarly, results showed that the survival rate of the hybrid grouper fed with transgenic Chlorella (55 ± 5% after 36 h) was much higher than that of fish fed with the same dosage of wild-type Chlorella (25 ± 5% after 36 h). Therefore, in vitro and in vivo results indicated that the constructed transgenic Chlorella with the marine AMPs Scy-hepc could exert effective protection for fish against the Aeromonas hydrophila infection, providing an encouraging prospect for the expected use of transgenic Chlorella in aquaculture in future.

Piston prover is widely used as a standard gas flow device for its advantages of high accuracy in standard volume, flow stability and repeatability. The applications of the conventional piston provers are limited by the maximum calibration flow generated by the piston cylinder volume. In the current paper, a reciprocating double-pistons gas prover was proposed, which can provide a continuous standard flow to calibrate meters like critical nozzles and so on. There are two pistons within the proposed prover, which could be operated in three modes: single-piston mode, double-pistons parallel mode and double-pistons reciprocating mode. The working mechanisms of all three modes were presented and the mathematical model was also introduced, especially for the double-pistons reciprocating mode. Then experiments were carried out to validate the new calibrating method; it is shown that the proposed reciprocating double-pistons gas prover is capable of precisely calibrating gas flow meters. Comparisons between the experimental results and data obtained using the piston prover based on the balance adjustment principle are also presented.

Existing piston gas provers use a single piston cylinder as standard which replace the standard flow of gas by the piston moving at a constant speed. In order to solve the problem that the piston proverflow ceiling is limited by the volume of piston cylinder, we have developed a double-piston gas prover. Firstly, the article introduces the devices principle and mathematical model simply. Then it presents a series of experiments and experiments results of the device. Finally,the paper analyzes the uncertainty of the equipment. The results show that the double-piston gas prover is available.

Although regional differences in cerebral volume have been revealed in developing human brains, little is known regarding the regionalization of cortical shape. This study documented the regional and quantitative shape difference of cortical surfaces for in utero normal fetal brains over a time period essential for the formation of primary cortical folding (22-33 weeks). Each brain surface with complete three-dimensional morphology was manually extracted from the reconstructed image, which combined surface information from three orthogonal magnetic resonance images in utero. An innovative parcellation was used to dissect the fetal brains into frontal, parietal, temporal and occipital lobes, and to avoid the determination of non-existent and immature sulci for young fetuses. Distinct cortical shapes were encoded by the shape index automatically. The results of this study show faster shape changes in the occipital lobe than in other regions. Both regional and global shape patterns show that the gyral surface smoothens, whereas the sulcal surface becomes more angular, with gestational age. In addition, the smoothing of gyri is related mainly to the changes in shape of gyral crowns. This study presents the regional differences in early gyrification from the novel aspect of shape. The results of shape pattern analysis for normal fetuses may act as a reference in assessments of prenatal brain pathology and in extensive comparisons between various life stages.

Although fractal analyses of adult human brain complexity have been performed for several years, the use of fractal dimension (FD) to measure complexity of the developing fetal cortical surface has not been extensively investigated. This study attempts to measure the complexity of the developing fetal cortical surface using the concept of FD. As has been done by others, the commonly used box-counting (BC) method was herein extended from 2-D to 3-D to quantify the FD of fetal cortical complexity. The primary theoretical contribution of this work is the modification of the 3-D BC method using a local FD measure to yield an accurate FD that elucidates the fractal characteristics of the cortical surface. The proposed 3-D modified BC (MBC) method was then adopted to estimate the FD to measure the complexity of the cortical surface of 32 normal and six test fetal brains at a gestational age (GA) of 27-37 weeks. The results for normal brains reveal that the increase in cortical complexity is correlated with the GA of the fetus. Observations of test brains indicate that the twins and cortical dysplasia are associated with lower FD than that of normal fetuses. These results suggest that the proposed 3-D MBC method is an effective means of measuring the complexity of the fetal cortical surface.

This study aims to investigate the complexity of developing fetal cortical surface using a 3D fast Fourier transform (FFT) fractal dimension (FD) estimator. Thirty-five fetal MR images were chosen at 22-36weeks of gestational age (GA) including 30 normal brains and 5 testing brains. Observations of testing brains indicate that: cortical dysplasia (developmental delay) and twins have lower FD than that of normal fetal brains. Results of FD from normal brains show that are positively correlated to GA and evidently indicate cortical complexity increasing with developing weeks of GA, which are in good agreement with fetal brain development. It could be said that the 3D FFT FD estimator is a promising means for the quantification of complexity of the fetal cortical surface.

The fetal cortical complexity is a significant quantification for assessing the development of fetal brain. This study attempts to quantify the development of fetal cortical complexity using the concept of fractal dimension (FD) analysis. Thirty-two fetal MR images were selected from Taipei Veterans General Hospital at 27–37 weeks of gestational age (GA). To investigate the FD of fetal cortical complexity, the entropy based information fractal dimension method (FDEBI), which is modified from Box-Counting method, was adopted and extended from 2D to 3D. The FD results from overall whole fetal brains show that the increase of cortical complexity is highly correlated with the gestational age of the fetus. Moreover, the FD values of right hemispheric brain are larger than those of left hemispheric brain, show that the development of right hemispheric fetal cortical complexity earlier than the left. These results are in good agreement with normal fetal brain development and suggest that the FD is an effective means for the quantification of fetal cortical complexity.

Regional differences in human brain development during infancy have been studied for many years, but little is known about how regionalization of the brain proceeds during intrauterine life. We investigated the regionalization of cerebral volume and cortical convolutions based on the volumetric magnetic resonance images (MRIs) of 43 fetuses, ranging from 21 to 37 weeks of gestation. Two plausible parcellations of MRI are proposed, and curvature index together with gyrification index are used to quantify the regional cortical convolutions. Our results elucidate that the cortical foldings among different brain regions develop at comparable rates, suggesting a similar uniformity of changes in size of the cortical sheet in these regions over time. On the contrary, the growth of the cerebral volume presents regional difference, with the frontal and parieto-temporal regions growing significantly faster than other regions due to the contribution from expansion of basal ganglia. This quantitative regional information suggests that cerebral volume is not a relevant parameter to measure in relation to gyrification, and that the size of the cortical sheet is more likely to be directly related to cortical folding. The availability of quantitative regional information on normal fetal brains in utero will allow clinical application of this information when probing neurodevelopmental disorders in the future.

Mass customization is a strategy that has been adopted by companies to tailor their products in order to match customer needs more precisely. Therefore, to fully capture the value of mass customization, it is crucial to explore how customers react to mass customization. In previous studies, an implied premise has been that consumers are keen to embrace customized products, and this assumption has also been treated by firms as a prerequisite for successful mass customization strategies. However, an undesirable complexity may result from difficult configuration processes that may intimidate and confuse some customers. Hence, this study explores strategies that marketers can employ to facilitate the customization process. Specifically, this study investigates how to enhance customer satisfaction and purchase decision toward customized products by providing cues compatible with the product category. It is hypothesized that for search products, customers rely more on intrinsic cues when making configuration decisions. On the other hand, for experience products, customers perceive extrinsic cues to be more valuable in assisting them to make configuration decisions. The results suggest that consumers tend to respond more favorably toward customized search products when intrinsic cues are provided than when extrinsic or irrelevant ones are provided. In contrast, when customizing experience products, customers tend to depend more on extrinsic cues than on intrinsic or irrelevant ones.

In this paper, the role of mixed amino acids in nitrate uptake and assimilation was evaluated in leafy radish by using 15N labeled nitrate. The mixtures of alanine, β-alanine, aspartic acid, asparagines, glutamic acid, glutamine, and glycine were sprayed to plant leaf two or four times. The activity of the enzymes related to the process of NO3(superscript -) reduction (nitrate reductase, nitrite reductase and glutamine synthetase) was affected differently depending on the application rate of mixed amino acids. Applying mixed amino acids increased the fresh weight, dry weight, and N yield. The NO3(superscript -) content was reduced to 24-38%, but no significant differences were observed in amino acids and proteins. In addition, the nitrogen derived from fertilizer and the 15N-NO3(superscript -) recovery rate increased to 2-8% and 15-47%, respectively. These results strongly suggest that the positive effect of mixed amino acids on nitrate uptake and assimilation might be attributed to the regulation on NO3(superscript -) uptake and assimilation, but not to the preference for amino acids as sources of reduced nitrogen.

Scygonadin is a novel antimicrobial peptide, which was originally isolated from the seminal plasma of the mud crab, Scylla serrata. Based on the partial 20-residue NH(2)-terminal sequence of the peptide, H-Gly-Gln-Ala-Leu-Asn-Lys-Leu-Met-Pro-Lys-Ile-Val-Ser-Ala-Ile-Ile-Tyr-Met-Val-Gly-OH, scygonadin was cloned from the gonads of S. serrata using a degenerated reverse transcriptase (RT)-PCR and rapid amplification of cDNA ends (RACE). The full-length cDNA sequence contains an open reading frame of 539 bases (excluding polyA) with a coding capacity of 126 amino acids, which constitutes a putative NH(2)-terminal signal sequence (1-24) and a mature peptide (25-126). Analysis of the genomic DNA sequence revealed that scygonadin consists of 2300 bp containing two introns (1569 and 120 bp) and three exons (187, 131 and 218 bp) and this sequence is different from any other reported antimicrobial peptide. The theoretical pI of the mature peptide is 6.09, which suggests that it is an anionic molecule. The sex and tissue-specific expression of the scygonadin gene was revealed using RT-PCR and Northern-blot analysis of multiple tissues of S. serrata males and females and this demonstrated that the scygonadin gene was predominantly expressed in the male reproductive tract of S. serrata and was restricted to the ejaculatory duct. This suggests that scygonadin might be one of the antibacterial peptides responsible for protection of the male crab reproductive tract from invading pathogenic microorganisms, so as to maintain a sterile environment leading to successful fertilization.

Innexins are a class of transmembrane proteins that are important for embryonic development, morphogenesis and electrical synapse formation. In the present study, a novel innexin2 gene from Scylla paramamosain was named Sp-inx2 and characterized. The complete cDNA and genomic DNA sequences of Sp-inx2 were revealed. Sp-inx2 mRNA transcripts were distributed in various tissues of S. paramamosain and were most abundant in the hemocytes. The Sp-inx2 was significantly upregulated in hemocyte, gill and hepatopancreas tissues with the challenge of either Vibrio alginolyticus, Vibrio parahaemolyticus or lipopolysaccharides (LPSs) when analyzed at 3 and 6 h using quantitative real-time PCR, suggesting that it could activate an immune response against the challenge of LPSs or Vibrio species. Using the chemical inhibitors carbenoxolone and probenecid, the absorption of the fluorescent dye Lucifer yellow decreased in the primary cultured hemocytes of crabs, thus confirming that hemichannels composed of Sp-inx2 existed in the crab hemocytes. With LPS stimulation, the level of mRNA transcripts and protein expression of Sp-inx2 in the same cultured hemocytes gradually increased from 6 to 48 h, while the activity of hemichannels was down-regulated at 6 and 12 h, demonstrating that LPSs could modulate the absorption activity of hemichannels in addition to its upregulation of Sp-inx2 gene expression. Furthermore, the dye uptake rate in HeLa cells in which Sp-inx2 was ectopically expressed increased dramatically but the increase was significantly down-regulated with the addition of 50 μg mL(-1) LPS, suggesting that the LPS stimulation could effectively reduce the activity of hemichannels. Interestingly, with the ectopic expression of Sp-inx2 in HeLa and EPC cells, apoptosis spontaneously occurred in both cultured cell lines when detected using TUNEL assay. In summary, a new Sp-inx2 gene was first characterized in a marine animal S. paramamosain and it had a function associated with immune response and cell apoptosis.

Pathogens can enter their host cells by way of endocytosis in which the membrane lipid raft gene flotillins are probably involved in the invasion process and this is an important way to cause infection. In this study, a new gene SpFLT-1 was identified in Scylla paramamosain, which shared high identity with the flotillin-1 of other species. The SpFLT-1 gene was widely distributed in tissues and showed the highest level of mRNA transcripts in the hemocytes. This gene might be a maternal gene based on the evident results that it was highly expressed in maternal ovaries and in the early developmental stages of the zygote and early embryo stage whereas it gradually decreased in zoea 1. SpFLT-1 positively responded to the challenge of Vibrio alginolyticus with a significantly increased level of mRNA expression in the hemocytes and gills at 3 hours post infection (hpi). The SpFLT-1 protein was detected densely in the same fraction layer where the Vibrio protein was most present in the hemocytes and gills at 3 hpi. Furthermore, it was found that the expression of SpFLT-1 decreased to the base level following disappearance of the Vibrio protein at 6 hpi in the gills. Silencing SpFLT-1 inhibited the endocytosis rate of V. alginolyticus but overexpression of the gene could facilitate bacterial entry into the epithelioma papulosum cyprinid cells. Our study indicated that SpFLT-1 may act as a key protein involved in the process of bacterial infection and this sheds light on clarifying the pathogenesis of pathogens infecting S. paramamosain.

We report here two new, high purity, high yield, one-step syntheses of cis -[Rh(bpy) 2 X 2 ][PF 6 ] {X = Cl, Br, I, bpy = 2,2′-bipyridine} directly from the RhX 3 · n H 2 O starting materials – one conventional and one microwave. The key to obtaining the pure complexes appears to be maintaining a 2:1 ratio of bipyridine to rhodium in solution; thus all reactants must be completely dissolved prior to the start of the reaction. Comparison of the two methods is also discussed. These complexes are pure enough for emission spectroscopy after minimal work-up. The complete characterization of all the halide complexes and the first cyclic voltammetry data on the cis -[Rh(bpy) 2 I 2 ][PF 6 ] complex are reported. The irreversible Rh(III)–Rh(I) redox potential becomes more positive from Cl to I. All three complexes show two reversible redox potentials corresponding to the bipyridine reductions. These data are consistent with the loss of the two halide ligands and formation of [Rh(bpy) 2 ] + upon reduction of Rh(III) to Rh(I).

The 3-D morphological change has gained increasing significance in recent investigations on human fetal brains. This study uses a pair of new indices, the shape index (SI) and curvedness index (CVD), to quantify 3-D morphological changes in developing brains from 22 to 33 weeks of gestation. The SI was used to automatically locate the gyral nodes and sulcal pits, and the CVD was used to measure the degree of deviation of cortical shapes from a flat plane. The CVD values of classified regions were compared with two traditional biomarkers: cerebral volume and cortical surface area. Because the fetal brains dramatically deform with age, the age effect was controlled during the comparison between morphological changes and volume and surface area. The results show that cerebral volume, the cortical surface area, and the CVD values of gyral nodes and sulcal pits increased with gestational age. However, with age controlled, the CVD values of gyral nodes and sulcal pits did not correlate with cerebral volume, but the CVD of gyral nodes increased slightly with the cortical surface area. These findings suggest that the SI, in conjunction with the CVD, provides developmental information distinct from the brain volumetry. This approach provides additional insight into 3-D cortical morphology in the assessment of fetal brain development.

Although regional differences in cerebral volume have been revealed in developing human brains, little is known regarding the regionalization of cortical shape. This study documented the regional and quantitative shape difference of cortical surfaces for in utero normal fetal brains over a time period essential for the formation of primary cortical folding (22-33 weeks). Each brain surface with complete three-dimensional morphology was manually extracted from the reconstructed image, which combined surface information from three orthogonal magnetic resonance images in utero. An innovative parcellation was used to dissect the fetal brains into frontal, parietal, temporal and occipital lobes, and to avoid the determination of non-existent and immature sulci for young fetuses. Distinct cortical shapes were encoded by the shape index automatically. The results of this study show faster shape changes in the occipital lobe than in other regions. Both regional and global shape patterns show that the gyral surface smoothens, whereas the sulcal surface becomes more angular, with gestational age. In addition, the smoothing of gyri is related mainly to the changes in shape of gyral crowns. This study presents the regional differences in early gyrification from the novel aspect of shape. The results of shape pattern analysis for normal fetuses may act as a reference in assessments of prenatal brain pathology and in extensive comparisons between various life stages.

In the current consumer-centric economy, consumers increasingly desire the opportunity to design their own products in order to express more effectively their self-image. Mass customization, based on efficient and flexible modulization designs, has provided individualized products to satisfy this desire. This work presents an experiment employed to demonstrate that customer participation leads to higher satisfaction. Specifically, the increment in customer satisfaction due to participation is greater when an easy example is provided than when either no example or a difficult one is provided. Additionally, self-congruity plays a mediating role on the customer participation-satisfaction relationship, and this mediating effect varies across different levels of the design example provided in the design process. When an easy design example is present, customer participation has a direct effect on satisfaction, in addition to the indirect effect of self-congruity. When a difficult example is provided, customer participation does not have incremental effects on either self-congruity or customer satisfaction. Finally, when no design example is shown to customers, contrary to our expectation, participation still enhances customer satisfaction due to an increased sense of self-congruity.

The partial and complete periodic synchronization in coupled discontinuous map lattices consisting of both discontinuous and non-invertible maps are discussed. We classify three typical types of periodic synchronization states, which give rise to different spatiotemporal patterns including static partial periodic synchronization, dynamically periodic synchronization, and complete periodic synchronization patterns. A special prelude dynamics of partial and complete periodic synchronization motion, which is shown by five separated concave curves in the time series plots of the order parameters, is observed. The detailed analysis shows that the special prelude dynamics is induced by the competition between two synchronized clusters, and the analytical expression for the corresponding order parameter is obtained.