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In order to select the varieties suitable for processing blueberry crispy, the quality evaluation of freeze-dried blueberry crispy was carried out. In this study, eight Highbush blueberries and Rabbiteye blueberries varieties in Jiangsu Province were used as raw materials, and were prepared as blueberry crispy by vacuum freeze-drying, and 18 quality indexes such as water content, total sugar, scavenging ability of DPPH free radical, hardness, etc, were determined, and the quality of the blueberry crispy was comprehensively evaluated by principal component analysis. The results showed that the coefficients of variation of sugar-acid ratio and total acid of different varieties of blueberry crispy were larger, with the value of 57.89% and 40.25%, respectively. Principal component analysis showed that the glucose content, anthocyanoside content, total flavonoid content, total antioxidant activity of AEAC, moisture content of dry samples, output rate and hardness were determined as representative indexes for the evaluation of the quality of blueberry brittle grains. Then, the comprehensive quality evaluation model was established. Based on the comprehensive quality score of each variety, the top three blueberry varieties were Legacy, Brightwell and Climax, and the others varieties had negative scores. This indicated that these three kinds of blueberries were more suitable for dry processing as blueberry crispy, among which the Legacy variety was the better. The results of the study provide a basis for the screening and quality analysis of blueberry varieties for dry processing.

To investigate the effects of different microwave drying conditions on the texture and nutritional quality of candied strawberries in a vacuum environment. This study investigated the effects of three different vacuum microwave treatment conditions (2.5 w/g-1.5 h, 3.5 w/g-1 h, 4.5 w/g-0.5 h) on moisture migration characteristics, texture, microstructure, color, anthocyanins, and phenolics of candied strawberries, which were evaluated by hot air drying. The results showed that the time required for vacuum microwave drying to dry candied strawberries to 20%±2% moisture content was significantly lower than that of hot air, and the higher the microwave power, the shorter the drying time. In addition, the water in the candied strawberries obtained after drying mainly appeared in two states, namely, bound water and water not easy to flow. The relaxation time T2 of strawberry candied strawberries obtained by vacuum microwave drying increased with the increase of microwave power, which indicated that the degree of freedom of water increased. Cross-sectional microscopic observation found that vacuum microwave drying caused cell wall rupture, and the higher the microwave power, the closer the site of fissure formation to the medullary core, and the bigger the fissure, and the smaller the porosity, whereas the cellular integrity and the porosity of the samples dried by the hot air were higher than that of microwave drying. In addition, the hardness, viscosity, and a* value of vacuum microwave-dried dried strawberry preserves were lower than those of hot air-dried samples, but the L* value, total sugar, anthocyanin, and phenolic content were higher. In conclusion, vacuum microwave drying had higher drying efficiency and could better retain the nutrients such as anthocyanins in candied strawberries.

Abstract Objectives Knoxia roxburghii is a member of the madder (Rubiaceae) family. This plant is cultivated in different areas of China and recognized for its medicinal properties, which leads to its use in traditional Chinese medicine. The incidence of root rot was 10–15%. In June 2023, the causal agent of root rot on K. roxburghii was identified as Fusarium oxysporum. To the best of our knowledge, this is the first report of the complete genome of F. oxysporum strain ByF01 that is the causal agent of root rot of K. roxburghii in China. The results will provide effective resources for pathogenesis on K. roxburghii and the prevention and control of root rot on this host in the future. Data description To understand the molecular mechanisms used by F. oxysporum to cause root rot on K. roxburghii, strain ByF01 was isolated from diseased roots and identified by morphological and molecular methods. The complete genome of strain ByF01 was then sequenced using a combination of the PacBio Sequel IIe and Illumina sequencing platforms. We obtained 54,431,725 bp of nucleotides, 47.46% GC content, and 16,705 coding sequences.

Abstract Background Rhizosphere and endophytic fungi play important roles in plant health and crop productivity. However, their community dynamics during the continuous cropping of Knoxia valerianoides have rarely been reported. K. valerianoides is a perennial herb of the family Rubiaceae and has been used in herbal medicines for ages. Here, we used high-throughput sequencing technology Illumina MiSeq to study the structural and functional dynamics of the rhizosphere and endophytic fungi of K. valerianoides. Results The findings indicate that continuous planting has led to an increase in the richness and diversity of rhizosphere fungi, while concomitantly resulting in a decrease in the richness and diversity of root fungi. The diversity of endophytic fungal communities in roots was lower than that of the rhizosphere fungi. Ascomycota and Basidiomycota were the dominant phyla detected during the continuous cropping of K. valerianoides. In addition, we found that root rot directly affected the structure and diversity of fungal communities in the rhizosphere and the roots of K. valerianoides. Consequently, both the rhizosphere and endophyte fungal communities of root rot-infected plants showed higher richness than the healthy plants. The relative abundance of Fusarium in two and three years old root rot-infected plants was significantly higher than the control, indicating that continuous planting negatively affected the health of K. valerianoides plants. Decision Curve Analysis showed that soil pH, organic matter (OM), available K, total K, soil sucrase (S_SC), soil catalase (S_CAT), and soil cellulase (S_CL) were significantly related (p

Abstract The predictive power of B-type natriuretic peptide (BNP) and left ventricular ejection fraction (LVEF) is limited by its low specificity in patients with heart failure (HF). Discovery of more novel biomarkers for HF better diagnosis is necessary and urgent. ELABELA, an early endogenous ligand for the G protein-coupled receptor APJ (Apelin peptide jejunum, Apelin receptor), exhibits cardioprotective actions. However, the relationship between plasma ELABELA and cardiac function in HF patients is unclear. To evaluate plasma ELABELA level and its diagnostic value in HF patients, a total of 335 patients with or without HF were recruited for our monocentric observational study. Plasma ELABELA and Apelin levels were detected by immunoassay in all patients. Spearman correlation analysis was used to analyze the correlation between plasma ELABELA or Apelin levels and study variables. The receiver operating characteristic curves were used to access the predictive power of plasma ELABELA or Apelin levels. Plasma ELABELA levels were lower, while plasma Apelin levels were higher in HF patients than in non-HF patients. Plasma ELABELA levels were gradually decreased with increasing New York Heart Association grade or decreasing LVEF. Plasma ELABELA levels were negatively correlated with BNP, left atrial diameter, left ventricular end-diastolic diameter, left ventricular end-systolic diameter, and left ventricular posterior wall thickness and positively correlated with LVEF in HF patients. In contrast, the correlation between plasma Apelin levels and these parameters is utterly opposite to ELABELA. The diagnostic value of ELABELA, Apelin, and LVEF for all HF patients was 0.835, 0.673, and 0.612; the sensitivity was 62.52, 66.20, and 32.97%; and the specificity was 95.92, 67.23, and 87.49%, respectively. All these parameters in HF patients with preserved ejection fraction were comparable to those in total HF patients. Overall, plasma ELABELA levels were significantly reduced and negatively correlated with cardiac function in HF patients. Decreased plasma ELABELA levels may function as a novel screening biomarker for HF. A combined assessment of BNP and ELABELA may be a good choice to increase the accuracy of the diagnosis of HF.

The microbial communities in rhizosphere soil play important roles in plant health and crop productivity. However, the microbial community structure of rhizosphere soil still remains unclear. In this study, the composition, diversity and function of the microbial communities in the rhizosphere soil of healthy and diseased plants were compared using Illumina MiSeq high-throughput sequencing. The Sobs (richness) and Shannon (diversity) indices of the soil microbial communities were higher in the rhizospheres of 2- and 3-year-old susceptible plants than in those of the healthy plants. With the increase in planting time, the numbers of fungi tended to decrease, while those of the bacteria tended to increase. Fungal diversity could be used as a biological indicator to measure the health of Knoxia roxburghii. The microbial composition and differential analyses revealed that the rhizosphere soil infested with fungi had a higher relative abundance at the phylum level in Ascomycota and Basidiomycota, while the bacteria had a higher relative abundance of Chloroflexi and a lower relative abundance of Actinobacteriota. At the genus level, the rhizosphere soil infested with fungi had relatively more abundant unclassified_f__Didymellaceae and Solicoccozyma and relatively less abundant Saitozyma and Penicillium. The bacterial genus norank_f__Gemmatimonadaceae was the most abundant, while Arthrobacter was less abundant. In addition, the abundance of Fusarium in the fungal community varied (p = 0.001). It tended to increase in parallel with the planting years. Therefore, it was hypothesized that the change in the community composition of Fusarium may be the primary reason for the occurrence of root rot in K. roxburghii, and the change in the abundance of Fusarium OTU1450 may be an indication of the occurrence of root rot in this species. The community function and prediction analyses showed that the pathogenic fungi increased with the increase in planting years. In general, soil fungi can be roughly divided into three types, including pathotrophs, symbiotrophs, and saprotrophs. An analysis of the differences in the prediction of different rhizosphere functions showed that D and L were significantly different in the COG enrichment pathway of the K. roxburghii rhizosphere bacteria (p

The purpose of this study was to discover the quality properties of the commercially available candied strawberries. Fifteen samples with different brands were purchased, and the physicochemical parametes, such as color, texture, sugar and acid content were determined using colorimeter, texture analyzer and HPLC. In addition, sensory scores were evaluated using intensity scale method. The results showed that the moisture content of these preserved strawberries were between 12.49% and 23.86%, and the colorimetric values distribution ranges of L*, a* and b* were 23.25~34.39, 5.17~19.83, and 5.78~17.04, respectively. For the texture characters, there were significant differences (P

Durian contains rich flavor components that undergo complex changes during drying. In this study, durian was subjected to integrated freeze-drying (IFD), conventional freeze-drying (CFD), and hot air drying (AD). Compared with the fresh samples, those dried by IFD, CFD, and AD lost 11, 9, and 7 original volatile compounds, respectively, and generated 7, 6, and 8 new volatile compounds, respectively, and showed a rapid and then slow decreasing trend in the total content during drying. However, the types of amino acids and soluble sugars remained unchanged during each of the drying methods. Furthermore, volatile compounds showed a significant negative correlation with the majority of amino acids and a significant positive correlation with soluble sugars. The IFD samples had the highest content of volatile compounds, amino acids, and soluble sugars. Therefore, IFD is recommended as a preferable drying method for durian.

In order to clarify the content and structural changes of different pectin fractions in grape cell walls under ultrasonic treatment, the grapes were treated with different ultrasonic time and ultrasonic power in this study, and the content of pectin fractions, composition of monosaccharides and structural changes of grape cell walls were analyzed by means of carbazole sulfuric acid method, PMP pre-column derivatization, high-performance liquid gel chromatography , scanning electron microscopy, Fourier transform infrared spectroscopy and circular dichroism. Results showed that the highest and the lowest contents in fresh grape cell walls were alkali-soluble pectin (NSP) and chelate pectin (CSP), respectively, and they were 27.41 mg/g AIR and 8.25 mg/g AIR. The total pectin decreased after ultrasonic treatments, in which the water-soluble pectin (WSP) increased and the CSP and NSP decreased. A total of six monosaccharides were detected in three pectins, and the monosaccharides of different pectin were not the same. The galactose and arabinose were high in WSP, the glucuronic acid was the most abundant of CSP and the rhamnose were the highest in NSP. After ultrasonic treatment, the contents of monosaccharides decreased, while the composition did not change, and the main chain structure of pectin was no change, but the linear structure and the degree of branch chain were changed. With the increase of ultrasonic times and powers, the molecular weight of different pectins declined gradually. And in the microstructure showed a more loose morphology. In addition, ultrasonic treatment had an effect on the structure and chain conformation of CSP and NSP, which made their maximum response values shift, and the effect of ultrasonic power was more significant. In conclusion, ultrasonic treatment could reduce the pectin and monosaccharide contents in grape cell walls, and affect the molecular linear structure and molecular chain conformation of pectin. These results can provide theoretical basis for the quality change of grape products under ultrasonic treatment.

【Objective】Analyzing the key physical and chemical indexes that affects the sensory quality of middle tobacco leaves (C3F grade) in Weifang, which can provide a certain theoretical basis for further improving the sensory quality of tobacco leaves in Weifang tobacco area.【Method】The key phenotypic, sensory and chemical parameters of the middle tobacco leaves of Linqu and Zhucheng from 2019 to 2021 were determined by physical measurement, chemical detection and sensory evaluation.【Result】The sensory evaluation of tobacco in middle leaves of Weifang was good, compared with Linqu tobacco area, the leaf length, leaf width, leaf weight, leaf thickness and leaf surface density of middle leaves in Zhucheng tobacco area was increased by 5.45%, 7.31%, 25.42%, 15.36% and 12.46%, respectively, while the stem content was decreased by 11.32%. The content of reducing sugar, total sugar, starch, nitrogen and chlorine in Zhucheng tobacco was increased by 9.83%, 6.23%, 26.12%, 7.9% and 291% compared to Linqu tobacco area, but the ratio of potassium to potassium chloride was significantly lower than that in Linqu tobacco area. The canonical correlation analysis of sensory quality and chemical indexes of tobacco leaves respectively obtained 1 and 0 pair canonical variables in Linqu and Zhucheng respectively, the results showed that the indexes of sensory quality which have a great influence on the canonical variables in Linqu were impurity, aftertaste and combustibility, and the chemical indexes were two-sugar ratio, chlorine content, potassium-chlorine ratio and total nitrogen content in leaves. The canonical correlation analysis of sensory evaluation and physical indexes of tobacco leaves respectively obtained 1 and 2 pair canonical variables in Linqu and Zhucheng respectively, the results showed that the indexes of sensory quality which have a great influence on the canonical variables of Linqu were aroma amount, gray amount, aftertaste amount and impurity, and the physical index was leaf length. The sensory quality which had great influence on the canonical variables of Zhucheng were combustibility, fragrance and total score, and the physical indexes was single leaf weight, leaf length and leaf width. Further correlation analysis of the above representative indicators showed that the aftertaste of Linqu tobacco leaves was promoted by nicotine content, while was inhibited by sugar-base ratio, miscellaneous gas was inhibited by chlorine content and aroma quantity was inhibited by leaf thickness and leaf surface density. The aroma of Zhucheng tobacco leaves was promoted by leaf density, and the burning ability was inhibited by the increase of leaf length, leaf width and single leaf weight.【Conclusion】The changes of physicochemical indexes of middle tobacco leaves significantly affected its sensory quality in Weifang tobacco area, in which, the representative physical indexes were leaf length, leaf weight and leaf width, the representative chemical indexes were the ratio of two sugars, chlorine, potassium/chlorine ratio and total nitrogen content, and the representative sensory indexes were impurities, aftertaste, combustibility, aroma and gray. In addition, it is suggesting that further increasing the reducing sugar, potassium content and sugar-alkali ratio of middle tobacco leaves in Weifang, and reducing the chlorine content of middle tobacco leaf in Zhucheng.

In order to discover the moisture distribution and migration during the drying process of strawberries at constant or gradient temperatures and the effect on the quality of dried strawberries, the strawberries which had experienced osmotic dehydration were dried under constant temperatures (60 °C-12 h, flip it over when dry to 6 h) and gradient temperature (65 °C-2 h, 60 °C-2 h, 55 °C-2 h, flip it over, 65 °C-2 h，60 °C-2 h，55 °C-2 h) during hot air drying. Low-field nuclear magnetic resonance (LF-NMR) was used and the physicochemical (texture, VC, total phenols) and sensory qualities (flavor, texture, taste, color) of the preserved strawberries obtained by the two drying programs were compared. The results showed that the water content of the dry base of strawberries at both programs decreased with time extending gradually, but the rate of gradient temperature was higher than that of constant temperature drying. From the T2 inversion spectra and peak areas of LF-NMR, it illustrated that the free water was the main form of water in the osmotically dehydrated strawberries, and there were also a small amount of bound water and immobile water. With the drying procedure proceeded, the T2 peak of the strawberry gradually decreases, which indicates a decrease in the mobility of the water. However, the total relaxation peak area of constant temperature drying and gradient drying was reduced to 31.3% and 26.4% of the original at the end of the drying programs, which showed that the gradient drying had higher drying efficiency. Magnetic resonance imaging (MRI) showed that the water in strawberries was firstly enriched to the broken tip of the fruit and dispersed, then in the medium of the drying process, the peel took the main position for the water evaporation, and finally some water left in the pith. From the imaging results, the main difference between the two drying programs was that there was an obvious homogeneous wetness phenomenon in the strawberries after 6 h drying i.e. the water was redistributed evenly in the fruit at that time, while this process did not appeared in the gradient drying. In addition, the VC and total phenolic contents of dried strawberries were significantly higher (P

IntroductionThree members of Capripoxvirus (CaPV) genus, including lumpy skin disease virus (LSDV), goatpox virus (GTPV), and sheeppox virus (SPPV), are mentioned as notifiable forms by World Organization for Animal Health. These viruses have negatively impacted ruminant farming industry worldwide, causing great economic losses. Although SPPV and GTPV cause more severe clinical disease in only one animal species, they can transfer between sheep and goats. Both homologous and heterologous immunization strategies are used to protect animals against CaPVs. However, development of accurate and rapid methods to distinguish these three viruses is helpful for the early detection, disease surveillance, and control of CaPV infection. Therefore, we developed a novel triplex real-time PCR (qPCR) for the differentiation of LSDV, GTPV, and SPPV.MethodsUniversal primers were designed to detect pan-CaPV sequences. Species-specific minor groove binder (MGB)-based probes were designed, which were labeled with FAM for LSDV, HEX for GTPV, and ROX for SPPV. The sensitivity, specificity, reproducibility, and ability of detecting mixed infections were evaluated for the triplex qPCR. Further, 226 clinical samples of the infection and negative controls were subjected to the triplex qPCR, and the results were verified using PCR-restriction fragment length polymorphism (PCR-RFLP) and sequencing methods for PRO30 gene.ResultsThe triplex qPCR could successfully distinguish LSDV, GTPV, and SPPV in one reaction, and the assay sensitivity was 5.41, 27.70, and 17.28 copies/μL, respectively. No cross-reactivity was observed with other viruses causing common ruminant diseases, including des petits ruminants virus, foot-and-mouth disease virus, bluetongue virus, ovine contagious pustular dermatitis virus, infectious bovine rhinotracheitis virus, and bovine viral diarrhea-mucosal disease virus. Inter-and intra-assay variabilities were < 2.5%. The results indicated that the triplex qPCR was highly specific, sensitive, and reproducible. Simulation experiments revealed that this assay could successfully distinguish two or three viruses in case of mixed infections without any cross-reaction. For clinical samples, the results were completely consistent with the results of PCR-RFLP and sequencing. This demonstrated that the assay was reliable for clinical application.DiscussionThe triplex qPCR is a robust, rapid, and simple tool for identifying various types of CaPV as it can successfully distinguish LSDV, GTPV, and SPPV in one reaction. Furthermore, the assay can facilitate more accurate disease diagnosis and surveillance for better control of CaPV infection.

Freeze-dried restructured strawberry blocks (FRSB) have become an increasingly popular product. In this study, the effects of six edible gums (guar gum, gelatin, xanthan gum, pectin, konjac gum, and carrageenan) on the FRSB quality were investigated. For FRSBs, compared with those in untreated samples, the 0.6 % guar gum addition increased texture profile analysis (TPA) hardness, chewiness, and puncture hardness by 29.59%, 174.86%, and 25.34%, respectively; after the 0.6% gelatin addition, the sensory evaluation sourness was reduced by 8.58%, whereas yield, TPA chewiness, and puncture hardness were increased by 3.40%, 28.62%, and 92.12%, respectively; with the 0.9% gelatin addition, the sensory evaluation sourness was reduced by 8.58%; with the 0.9% pectin addition, the yield, TPA hardness, chewiness, and puncture hardness were increased by 4.55%, 5.94%, 77.49%, and 103.62%, respectively. In summary, 0.6–0.9% pectin, gelatin, and guar gum addition are recommended to improve the main qualities of FRSBs.

African swine fever (ASF) is a lethal contagious viral disease of domestic pigs and wild boars caused by the African swine fever virus (ASFV). The pandemic spread of ASF has caused severe effects on the global pig industry. Whole-genome sequencing provides crucial information for virus strain characterization, epidemiology analysis and vaccine development. Here, we evaluated the performance of next-generation sequencing (NGS) in generating ASFV genome sequences from clinical samples. Thirty-four ASFV-positive field samples including spleen, lymph node, lung, liver and blood with a range of Ct values from 14.73 to 25.95 were sequenced. For different tissue samples collected from the same sick pigs, the proportion of ASFV reads obtained from the spleen samples was 3.69–9.86 times higher than other tissues. For the high-viral-load spleen samples (Ct < 20), a minimum of a 99.8% breadth of ≥10× coverage was revealed for all the samples. For the spleen samples with Ct ≥ 20, 6/12 samples had a minimum of a 99.8% breadth of ≥10× coverage. A high average depth of sequencing coverage was also achieved from the blood samples. According to our results, high-quality ASFV whole-genome sequences could be obtained from the spleen or blood samples with Ct < 20. The high-quality ASFV genome sequence generated in this study was further used for the high-resolution phylogenetic analysis of the ASFV genomes in the early stage of the ASF epidemic in China. Our study demonstrates that NGS may act as a useful tool for efficient ASFV genome characterization, providing valuable information for disease control.

In order to explore the water diffusion characteristics of green bananas during the microwave drying process, a microwave drying test was conducted to investigate the green bananas drying characteristics under different microwave power densities (3, 5, 7 and 9 W/g), and the microwave drying test model of green bananas was established. Low-field nuclear magnetic resonance (LF-NMR) was used to investigate the internal moisture distribution and change law of green bananas during the drying process. Results showed that drying rate of green bananas in the microwave drying process increased first and then decreased, the effective diffusion coefficient was between 1.082×10−8 and 7.708×10−8 m2/s, and also increased with the increasing of microwave power density. The nuclear magnetic resonance experiments showed that, during the drying process, the free water was removed first, the non-flowing water showed an overall upward trend and then a downward trend, while the combined water showed an overall upward trend. With the extension of microwave drying time, the water peak area of the three states decreased and the total peak moved in the direction of the decrease of relaxation time, at the end of drying, the free water in green banana was basically removed, and the internal water mainly existed in the state of combined water and non flowing water. By fitting green banana drying kinetics model, it was found that the Page model fitted best (R2>0.9), which could provide a basis for effectively describing the variation law of water with time during the microwave drying process of green bananas.

Atrial fibrillation (AF) is commonly prevalent in patients with hypertrophic cardiomyopathy (HCM). However, whether the prevalence and incidence of AF are different between genotype-positive vs. genotype-negative patients with HCM remains controversial. Recent evidence has indicated that AF is often the first presentation of genetic HCM patients in the absence of a cardiomyopathy phenotype, implying the importance of genetic testing in this population with early-onset AF. However, the association of the identified sarcomere gene variants with HCM occurrence in the future remains unclear. How the identification of these cardiomyopathy gene variants should influence the use of anticoagulation therapy for a patient with early-onset AF is still undefined. In this review, we sought to assess the genetic variants, pathophysiological pathways, and oral anticoagulation in patients with HCM and AF.

Snake venom is considered a “toxin arsenal”, and it often induces a series of clinical and pathophysiological symptoms in snakebite victims. Interestingly, toxin inhibitors are commonly found in the serum of snakes and their predators. Sinonatrix annularis is a type of non-venomous snake that was reported to contain an “inhibitor cocktail”, including phospholipase A2 inhibitors (PLIs), metalloproteinase inhibitors (SVMPIs), and small serum protein (SSP). However, the sequences and activities of these components remain obscure. In this study, we performed envenomation challenges on S. annularis using venoms from Deinagkistrodon acutus, Agkistrodon halys and Naja atra. In brief, the maximum injected amount of venom was 360 mg/kg for D. acutus, 72 mg/kg for A. halys, and 18 mg/kg for N. atra. The mRNA expression of the inhibitors PLIα, PLIβ, PLIγ, SVMPI, serpin A1, and SSP showed a dose-dependent effect on envenomation. Liver homogenate from S. annularis (LH) was prepared and used to evaluate its inhibitory effect on snake venoms. As a result, LH showed significant neutralization of venom PLA2, mitigated hemorrhage, venom-induced muscle damage, and system toxicity. In the presence of LH, envenomated mice exhibited attenuated inflammation, apoptosis, oxidative damage, and mitigated changes in serum biochemical markers caused by venom. The study reveals the secret of “natural immunity” in snakes, namely, the “antivenom”, which consists of an inhibitor proteome or cocktail.

Abstract Edible fungi are rich in nutrition, but they are susceptible to spoilage, and often prolonged by drying. RF and microwave energy drying have the advantages of short drying time, high energy efficiency and good process control. However, to develop an effective dielectric drying method, it is important to understand dielectric properties, the major factor characterizing the interaction between the electromagnetic energy and the food. At present, there is a lack of research on dielectric properties of edible fungi. In this study, a vector network analyzer and an open-ended coaxial-line probe were employed to measure the dielectric parameters. The dielectric parameters were observed at different temperatures (25–85 °C) for edible fungi powder with moisture content ranging from 5 to 30% wet basis over a frequency range of 1–3000 MHz. The relationship between the dielectric properties and frequency, temperature, and moisture content were obtained via regression analysis. Further, the dielectric penetration depth was calculated, and the effects of frequency, moisture content, and temperature on the penetration depth were also analyzed. The results showed that the dielectric properties of edible fungi powder increased with an increase in moisture content and temperature, while they decreased with increasing frequency. At high moisture content and temperature, the increase in dielectric properties was slightly larger than that at low moisture content and temperature. The dielectric properties changed more evidently at lower radio frequencies than at higher radio frequencies. The penetration depth decreased with an increase in temperature, moisture content, and frequency. It can be concluded that a large penetration depth at radio frequencies below 100 MHz could be used to dry edible fungi on a large scale, whereas microwave energy could be employed for drying edible fungi on a small scale.

Dielectric properties of sliced Agaricus bisporus samples, with 0.16–0.89 [g/g, wet basis (w.b.)] moisture content, were determined using a network analyser and an open-ended coaxial-line probe, over a frequency range of 915 MHz to 2450 MHz and a temperature range of 25°C to 60°C. The results showed that the permittivity of A. bisporus was a function of frequency, moisture content, and temperature. The dielectric constant and loss factor were directly proportional to moisture content and temperature, respectively, and both decreased with increasing frequency. However, the influence of temperature on the dielectric constant showed a different trend, as this was affected by the moisture content of the sample. The dielectric loss factor initially increased and then decreased with the decrease in moisture content. Both inflection points appeared at the moisture content value of 0.69 (g/g, w.b.), which represents the “semi-bound water transfer” point. Third-degree polynomial models could be used to describe dielectric constant and loss factor values as functions of moisture content and temperature. The penetration depth decreased with increasing frequency and temperature; however, this first decreased with decreasing moisture content and then increased. Notably, the penetration depth was at a low ebb when the moisture content ranged from 0.69 to 0.52 (g/g, w.b.), indicating easier uneven drying. Low frequency (915 MHz) with large penetration depth represents a highly effective method for large-scale industrial dielectric microwave drying of A. bisporus slices from the angle of uniformity.

High hydrostatic pressure (HHP) can enhance the physicochemical properties of soluble dietary fiber (SDF) from fruit and vegetable residues including hydration properties, emulsibility, and rheological properties, while the pretreatment methods such as solid-water suspension status are ignored all along. Here, three groups of lotus root residue (LRR) for HHP treatment (400 MPa, 15 min) were prepared: the fresh lotus root residue (FLRR), FLRR mixed with water (FLRR + W), and dried FLRR suspended in water at the same solid/water level with FLRR + W (DLRR + W). As a control, non-HHP-treated FLRR was tested. Results showed that FLRR + W obtained the highest SDF yield and presented a honeycomb structure which was not observed in other LRR samples. In addition, properties of SDF extract from FLRR + W changed most significantly, including not only the enhancement of SDF yield, the improvement of hydration properties, and the reduction of molecular weight but also the increase of thermal and rheological stability. Principal component analysis (PCA) profile illustrated that the difference of LRR-water system contributed 27.6% to the SDF physicochemical changes, and SDF from DLRR + W distinguished it from the other samples with mannose, ribose, and glucuronic acid, indicating that the drying procedure also played a role in the HHP treatment focusing on the sugar constitution. Therefore, the solid-water suspension status is a noteworthy issue before HHP treatment aiming at SDF modification.

With its high moisture content and tender texture, fresh strawberry is very susceptible to mechanical damage and microbial infection. Drying is one of the most frequently employed methods to extend its shelf life, and freeze-dried restructured strawberry block (FRSB) is an emerging popular food. Here, in order to enhance the quality of FRSB, edible gums of guar gum, pectin, and gelatin were added and the combination was optimized using response surface methodology (RSM) with chewiness, hardness, and organoleptic evaluations of the dried sample as the response indicators. The results showed that the combination addition of 0.10% guar gum, 0.22% pectin, and 0.30% gelatin contributed to the highest comprehensive quality of the dried sample. Compared with the untreated sample, the optimal combination addition of the three edible gums resulted in a higher moisture content for the dried sample (increased by 0.8%), and increased the chewiness, hardness, and porosity by 82.04%, 27.09%, and 3.01%, respectively, while maintaining more original color and forming a denser porous microstructure. The findings in the current work will be useful for the application of edible gums in freeze-dried restructured fruits and vegetables.

Lemon juice vesicles have abundant flavor components that can undergo complex changes during drying. Three drying methods, including integrated freeze-drying (IFD), conventional freeze-drying (CFD), and hot-air drying (AD), were studied to determine their effects on the dynamic changes in the flavor compounds in lemon juice vesicles. Compared with the fresh samples, the final dried samples that underwent IFD, CFD, and AD lost seven, seven, and six volatile flavor compounds and three, four, and five amino acids, respectively; the order of the loss ratios with respect to the volatile compound content was: 82.73% in CFD > 71.22% in IFD > 28.78% in AD. AD resulted in the highest total amino acid content (10.83 ± 0.20 mg/g), which was 1.39 and 5.54 mg/g higher than that of IFD and CFD, respectively; CFD resulted in the highest total organic acid content (45.94 ± 0.34 mg/g), which was 8.01 and 7.87 mg/g higher than that of IFD and AD, respectively; and AD contributed to the highest total soluble sugars (17.12 ± 0.20 mg/g), which was 1.24 and 1.49 mg/g higher than that of IFD and CFD, respectively. A correlation analysis demonstrated that most of the amino acids and the soluble sugars were closely related to the profiles of the volatile compounds in the lemon juice vesicles during drying.

Small ruminant morbillivirus (SRMV), formerly known as peste-des-petits-ruminants virus, classified into the genus Morbillivirus in the family Paramyxoviridae. Its L protein functions as the RNA-dependent RNA polymerases (RdRp) during viral replication. Due to the absence of efficient proofreading activity in their RdRps, various RNA viruses reveal high mutation frequencies, making them evolve rapidly during serial passages in cells, especially treated with a certain mutagen, like ribavirin. We have previously rescued a recombinant enhanced green fluorescence protein-tagged SRMV (rSRMV-eGFP) using reverse genetics. In this study, the rSRMV-eGFP was subjected to serial passages in ribavirin-treated cells. Due to the ribavirin-exerted selective pressure, it was speculated that viral progenies would form quasispecies after dozens of passages. Viral progenies at passage-10, -20, -30, -40, and -50 were separately subjected to next-generation sequencing (NGS), consequently revealing a total of 34 single-nucleotide variations, including five synonymous, 21 missense, and one non-sense mutations. The L sequence was found to harbor eight missense mutations during serial passaging. It was speculated that at least one high-fidelity variant was present in viral quasispecies at passage-50. If purified from the population of viral progenies, this putative variant would contribute to clarifying a molecular mechanism in viral high-fidelity replication in vitro.

African swine fever (ASF), caused by African swine fever virus (ASFV), is a devastating infectious disease of domestic pigs and wild boars, and has tremendous negative socioeconomic impact on the swine industry and food security worldwide. It is characterized as a notifiable disease by World Organisation for Animal Health (OIE). No effective vaccine or treatment against ASF has so far been available. Early detection and rapid diagnosis are of potential significance to control the spread of ASF. Recombinase-based isothermal amplification assay, recombinase polymerase amplification (RPA) developed by TwistDx (Cambridge, United Kingdom) or recombinase-aided amplification (RAA) by Qitian (Wuxi, China), is becoming a molecular tool for the rapid, specific, and cost-effective identification of multiple pathogens. In this study, we aim to investigate if RPA/RAA can be a potential candidate for on-site, rapid and primary detection of ASFV. A panel of 152 clinical samples previously well-characterized by OIE-recommended qPCR was enrolled in this study, including 20 weak positive (Ct value ≥ 30) samples. This panel was consisted of different types, such as EDTA-blood, spleen, lung, lymph node, kidney, tonsil, liver, brain. We evaluated two recombinase-based isothermal amplification assays, RPA or RAA, by targeting the ASFV B646L gene (p72), and validated the clinical performance in comparison with OIE real-time PCR. Our result showed that the analytical sensitivity of RPA and RAA was as 93.4 and 53.6 copies per reaction, respectively at 95% probability in 16 min, at 39°C. They were universally specific for all 24 genotypes of ASFV and no cross reaction to other pathogens including Classical swine fever virus (CSV), Foot-and-mouth disease virus (FMDV), Pseudorabies virus, Porcine circovirus 2 (PCV2), Porcine Reproductive and respiratory syndrome virus (PPRSV). The results on detection of various kinds of clinical samples indicated an excellent diagnostic agreement between RPA, RAA and OIE real-time PCR method, with the kappa value of 0.960 and 0.973, respectively. Compared to real-time PCR, the specificity of both RPA and RAA was 100% (94.40% ∼ 100%, 95% CI), while the sensitivity was 96.59% (90.36% ∼ 99.29%, 95% CI) and 97.73% (92.03% ∼ 99.72%, 95% CI), respectively. Our data demonstrate that the developed recombinase-based amplification assay (RPA/RAA), promisingly equipped with field-deployable instruments, offers a sensitive and specific platform for the rapid and reliable detection of ASFV, especially in the resource-limited settings for the purpose of screening and surveillance of ASF.

On August 3, 2018, an outbreak of African swine fever in pigs was reported in China. We subjected a virus from an African swine fever–positive pig sample to phylogenetic analysis. This analysis showed that the causative strain belonged to the p72 genotype II and CD2v serogroup 8.

This paper investigates the problem of robust H∞ guaranteed cost control for non-linear uncertain switched singular systems with multiple time-delay. Nonlinear is considered to be norm bounded and the systems are regular and impulse free. A sufficient condition for existences of robust H∞ guaranteed cost controller is given by the linear matrix inequalities (LMI) at the Lyapunov function and switching rule. It is shown that the closed-loop systems are asymptotically stable. Finally, the results are validated and advantage, through a given example.

Serologic and molecular evidence indicates that peste des petits ruminants virus (PPRV) infection has emerged in goats and sheep in the Ngari region of southwestern Tibet, People’s Republic of China. Phylogenetic analysis confirms that the PPRV strain from Tibet is classified as lineage 4 and is closely related to viruses currently circulating in neighboring countries of southern Asia.