148 results on '"Ancuta, Petronela"'
Search Results
2. Retinoic acid enhances HIV-1 reverse transcription and transcription in macrophages via mTOR-modulated mechanisms
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Dias, Jonathan, Cattin, Amélie, Bendoumou, Maryam, Dutilleul, Antoine, Lodge, Robert, Goulet, Jean-Philippe, Fert, Augustine, Raymond Marchand, Laurence, Wiche Salinas, Tomas Raul, Ngassaki Yoka, Christ-Dominique, Gabriel, Etiene Moreira, Caballero, Ramon Edwin, Routy, Jean-Pierre, Cohen, Éric A., Van Lint, Carine, and Ancuta, Petronela
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- 2024
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3. Near full-length HIV sequencing in multiple tissues collected postmortem reveals shared clonal expansions across distinct reservoirs during ART
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Dufour, Caroline, Ruiz, Maria Julia, Pagliuzza, Amélie, Richard, Corentin, Shahid, Aniqa, Fromentin, Rémi, Ponte, Rosalie, Cattin, Amélie, Wiche Salinas, Tomas Raul, Salahuddin, Syim, Sandstrom, Teslin, Schinkel, Stephanie Burke, Costiniuk, Cecilia T., Jenabian, Mohammad-Ali, Ancuta, Petronela, Routy, Jean-Pierre, Cohen, Éric A., Brumme, Zabrina L., Power, Christopher, Angel, Jonathan B., and Chomont, Nicolas
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- 2023
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4. Identification of aryl hydrocarbon receptor as a barrier to HIV-1 infection and outgrowth in CD4+ T cells
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Chatterjee, Debashree, Zhang, Yuwei, Ngassaki-Yoka, Christ-Dominique, Dutilleul, Antoine, Khalfi, Soumia, Hernalsteens, Olivier, Wiche Salinas, Tomas Raul, Dias, Jonathan, Chen, Huicheng, Smail, Yasmine, Goulet, Jean-Philippe, Bell, Brendan, Routy, Jean-Pierre, Van Lint, Carine, and Ancuta, Petronela
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- 2023
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5. Th17 cell master transcription factor RORC2 regulates HIV-1 gene expression and viral outgrowth
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Salinas, Tomas Raul Wiche, Zhang, Yuwei, Sarnello, Daniele, Zhyvoloup, Alexander, Marchand, Laurence Raymond, Fert, Augustine, Planas, Delphine, Lodha, Manivel, Chatterjee, Debashree, Karwacz, Katarzyna, Oxenford, Sally, Routy, Jean-Pierre, Irlbeck, David, Amrine-Madsen, Heather, Ancuta, Petronela, and Fassati, Ariberto
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- 2021
6. Immuno-metabolic control of the balance between Th17-polarized and regulatory T-cells during HIV infection
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Yero, Alexis, Bouassa, Ralph-Sydney Mboumba, Ancuta, Petronela, Estaquier, Jerome, and Jenabian, Mohammad-Ali
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- 2023
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7. Targeting Th17 cells in HIV-1 remission/cure interventions
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Fert, Augustine, Raymond Marchand, Laurence, Wiche Salinas, Tomas Raul, and Ancuta, Petronela
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- 2022
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8. IL-17A reprograms intestinal epithelial cells to facilitate HIV-1 replication and outgrowth in CD4+ T cells
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Wiche Salinas, Tomas Raul, Gosselin, Annie, Raymond Marchand, Laurence, Moreira Gabriel, Etiene, Tastet, Olivier, Goulet, Jean-Philippe, Zhang, Yuwei, Vlad, Dragos, Touil, Hanane, Routy, Jean-Pierre, Bego, Mariana G., El-Far, Mohamed, Chomont, Nicolas, Landay, Alan L., Cohen, Éric A., Tremblay, Cécile, and Ancuta, Petronela
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- 2021
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9. IL-32 Drives the Differentiation of Cardiotropic CD4+ T Cells Carrying HIV DNA in People With HIV.
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Ramani, Hardik, Gosselin, Annie, Bunet, Rémi, Jenabian, Mohammad-Ali, Sylla, Mohamed, Pagliuzza, Amélie, Chartrand-Lefebvre, Carl, Routy, Jean-Pierre, Goulet, Jean-Philippe, Thomas, Réjean, Trottier, Benoit, Martel-Laferrière, Valérie, Fortin, Claude, Chomont, Nicolas, Fromentin, Rémi, Landay, Alan L, Durand, Madeleine, Ancuta, Petronela, El-Far, Mohamed, and Tremblay, Cecile
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T cells ,HIV-positive persons ,INTERLEUKIN-32 ,PROTEIN-tyrosine kinases ,IMMUNOLOGIC memory ,VIRAL tropism - Abstract
Interleukin 32 (IL-32) is a potent multi-isoform proinflammatory cytokine, which is upregulated in people with HIV (PWH) and is associated with cardiovascular disease (CVD) risk. However, the impact of IL-32 isoforms on CD4 T-cell cardiotropism, a mechanism potentially contributing to heart inflammation, remains unknown. Here we show that IL-32 isoforms β and γ induce the generation of CCR4
+ CXCR3+ double positive (DP) memory CD4 T-cell subpopulation expressing the tyrosine kinase receptor c-Met, a phenotype associated with heart-homing of T cells. Our ex vivo studies on PWH show that the frequency of DP CD4 T cells is significantly higher in individuals with, compared to individuals without, subclinical atherosclerosis and that DP cells from antiretroviral-naive and treated individuals are highly enriched with HIV DNA. Together, these data demonstrate that IL-32 isoforms have the potential to induce heart-homing of HIV-infected CD4 T cells, which may further aggravate heart inflammation and CVD in PWH. [ABSTRACT FROM AUTHOR]- Published
- 2024
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10. Phylogenetic analysis of HIV-1 archived DNA in blood and gut-associated lymphoid tissue in two patients under antiretroviral therapy
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Recordon-Pinson, Patricia, Gosselin, Annie, Ancuta, Petronela, Routy, Jean-Pierre, and Fleury, Hervé
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- 2021
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11. Alterations in Th17 Cells and Non-Classical Monocytes as a Signature of Subclinical Coronary Artery Atherosclerosis during ART-Treated HIV-1 Infection.
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Wiche Salinas, Tomas Raul, Zhang, Yuwei, Gosselin, Annie, Rosario, Natalia Fonseca, El-Far, Mohamed, Filali-Mouhim, Ali, Routy, Jean-Pierre, Chartrand-Lefebvre, Carl, Landay, Alan L., Durand, Madeleine, Tremblay, Cécile L., and Ancuta, Petronela
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T helper cells ,CORONARY artery disease ,MONOCYTES ,CORONARY arteries ,DISEASE risk factors ,HIV - Abstract
Cardiovascular disease (CVD) remains an important comorbidity in people living with HIV-1 (PLWH) receiving antiretroviral therapy (ART). Our previous studies performed in the Canadian HIV/Aging Cohort Study (CHACS) (>40 years-old; Framingham Risk Score (FRS) > 5%) revealed a 2–3-fold increase in non-calcified coronary artery atherosclerosis (CAA) plaque burden, measured by computed tomography angiography scan (CTAScan) as the total (TPV) and low attenuated plaque volume (LAPV), in ART-treated PLWH (HIV+) versus uninfected controls (HIV−). In an effort to identify novel correlates of subclinical CAA, markers of intestinal damage (sCD14, LBP, FABP2); cell trafficking/inflammation (CCL20, CX3CL1, MIF, CCL25); subsets of Th17-polarized and regulatory (Tregs) CD4
+ T-cells, classical/intermediate/non-classical monocytes, and myeloid/plasmacytoid dendritic cells were studied in relationship with HIV and TPV/LAPV status. The TPV detection/values coincided with higher plasma sCD14, FABP2, CCL20, MIF, CX3CL1, and triglyceride levels; lower Th17/Treg ratios; and classical monocyte expansion. Among HIV+ , TPV+ versus TPV− exhibited lower Th17 frequencies, reduced Th17/Treg ratios, higher frequencies of non-classical CCR9low HLADRhigh monocytes, and increased plasma fibrinogen levels. Finally, Th17/Treg ratios and non-classical CCR9low HLADRhigh monocyte frequencies remained associated with TPV/LAPV after adjusting for FRS and HIV/ART duration in a logistic regression model. These findings point to Th17 paucity and non-classical monocyte abundance as novel immunological correlates of subclinical CAA that may fuel the CVD risk in ART-treated PLWH. [ABSTRACT FROM AUTHOR]- Published
- 2024
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12. Daily variations of gut microbial translocation markers in ART-treated HIV-infected people
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Ouyang, Jing, Isnard, Stéphane, Lin, John, Fombuena, Brandon, Chatterjee, Debashree, Wiche Salinas, Tomas Raul, Planas, Delphine, Cattin, Amélie, Fert, Augustine, Moreira Gabriel, Etiene, Raymond Marchand, Laurence, Zhang, Yonglong, Finkelman, Malcolm, Chen, Yaokai, Kaufmann, Daniel E., Cermakian, Nicolas, Ancuta, Petronela, and Routy, Jean-Pierre
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- 2020
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13. Upregulation of IL-32 Isoforms in Virologically Suppressed HIV-Infected Individuals: Potential Role in Persistent Inflammation and Transcription From Stable HIV-1 Reservoirs
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Zaidan, Sarah M., Leyre, Louise, Bunet, Rémi, Larouche-Anctil, Etienne, Turcotte, Isabelle, Sylla, Mohamed, Chamberland, Annie, Chartrand-Lefebvre, Carl, Ancuta, Petronela, Routy, Jean-Pierre, Baril, Jean-Guy, Trottier, Benoit, MacPherson, Paul, Trottier, Sylvie, Harris, Marianne, Walmsley, Sharon, Conway, Brian, Wong, Alexander, Thomas, Réjean, Kaplan, Robert C., Landay, Alan L., Durand, Madeleine, Chomont, Nicolas, Tremblay, Cécile L., and El-Far, Mohamed
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- 2019
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14. New Th17-specific therapeutic strategies for HIV remission
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Planas, Delphine, Routy, Jean-Pierre, and Ancuta, Petronela
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- 2019
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15. Immunosuppressive Tryptophan Catabolism and Gut Mucosal Dysfunction Following Early HIV Infection
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Jenabian, Mohammad-Ali, El-Far, Mohamed, Vyboh, Kishanda, Kema, Ido, Costiniuk, Cecilia T., Thomas, Rejean, Baril, Jean-Guy, LeBlanc, Roger, Kanagaratham, Cynthia, Radzioch, Danuta, Allam, Ossama, Ahmad, Ali, Lebouché, Bertrand, Tremblay, Cécile, Ancuta, Petronela, and Routy, Jean-Pierre
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- 2015
16. Differential Impact of IL-32 Isoforms on the Functions of Coronary Artery Endothelial Cells: A Potential Link with Arterial Stiffness and Atherosclerosis.
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Bunet, Rémi, Roy-Cardinal, Marie-Hélène, Ramani, Hardik, Cleret-Buhot, Aurélie, Durand, Madeleine, Chartrand-Lefebvre, Carl, Routy, Jean-Pierre, Thomas, Réjean, Trottier, Benoît, Ancuta, Petronela, Hanna, David B., Landay, Alan L., Cloutier, Guy, Tremblay, Cécile L., and El-Far, Mohamed
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ARTERIAL diseases ,CORONARY arteries ,ENDOTHELIAL cells ,ENDOTHELIUM diseases ,INTERLEUKIN-32 ,ATHEROSCLEROSIS - Abstract
Chronic inflammation is associated with higher risk of cardiovascular disease (CVD) in people living with HIV (PLWH). We have previously shown that interleukin-32 (IL-32), a multi-isoform proinflammatory cytokine, is chronically upregulated in PLWH and is linked with CVD. However, the mechanistic roles of the different IL-32 isoforms in CVD are yet to be identified. In this study, we aimed to investigate the potential impact of IL-32 isoforms on coronary artery endothelial cells (CAEC), whose dysfunction represents a major factor for atherosclerosis. Our results demonstrated that the predominantly expressed IL-32 isoforms (IL-32β and IL-32γ) have a selective impact on the production of the proinflammatory cytokine IL-6 by CAEC. Furthermore, these two isoforms induced endothelial cell dysfunction by upregulating the expression of the adhesion molecules ICAM-I and VCAM-I and the chemoattractants CCL-2, CXCL-8 and CXCL-1. IL-32-mediated expression of these chemokines was sufficient to drive monocyte transmigration in vitro. Finally, we demonstrate that IL-32 expression in both PLWH and controls correlates with the carotid artery stiffness, measured by the cumulated lateral translation. These results suggest a role for IL-32-mediated endothelial cell dysfunction in dysregulation of the blood vessel wall and that IL-32 may represent a therapeutic target to prevent CVD in PLWH. [ABSTRACT FROM AUTHOR]
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- 2023
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17. Nomenclature of monocytes and dendritic cells in blood
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Ziegler-Heitbrock, Loems, Ancuta, Petronela, Crowe, Suzanne, Dalod, Marc, Grau, Veronika, Hart, Derek N., Leenen, Pieter J.M., Liu, Yong-Jun, MacPherson, Gordon, Randolph, Gwendalyn J., Scherberich, Juergen, Schmitz, Juergen, Shortman, Ken, Sozzani, Silvano, Strobl, Herbert, Zembala, Marek, Austyn, Jonathan M., and Lutz, Manfred B.
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- 2010
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18. Socio-economic status and time trends associated with early ART initiation following primary HIV infection in Montreal, Canada: 1996 to 2015
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Mehraj, Vikram, Cox, Joseph, Lebouché, Bertrand, Costiniuk, Cecilia, Cao, Wei, Li, Taisheng, Ponte, Rosalie, Thomas, Réjean, Szabo, Jason, Baril, Jean?Guy, Trottier, Benoit, Côté, Pierre, Leblanc, Roger, Bruneau, Julie, Tremblay, Cécile, Routy, Jean?Pierre, Charest, L., Milne, C., Lavoie, S., Friedman, J., Duchastel, M., Villielm, F., Asselin, F., Boissonnault, M., Maziade, P.J., Milne, M., Lessard, B., Charron, M.A., Dufresne, S., Turgeon, M.E., Vezina, S., Huchet, E., Kerba, J.P., Poliquin, M., Poulin, S., Rochette, P., Junod, P., Longpré, D., Pilarski, R., Sasseville, E., Labrecque, L., Fortin, C., Hal?Gagne, V., Munoz, M., Deligne, B., Martel?Laferriere, V., Goyer, M.E, Gilmore, N., Potter, M., Klein, M., Teltscher, M., Pokomandy, A., Haraoui, L.P., Rivet, Nathalie, Nguyen, Tuyen, Bernard, Nicole, Dupuy, Franck, Cohen, Eric A., Ancuta, Petronela, Roger, Michel, Wainberg, Mark A., and Brenner, Bluma G.
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Medical care, Cost of -- Analysis ,Antiviral agents -- Dosage and administration ,HIV patients -- Care and treatment ,HIV infection -- Care and treatment ,Health - Abstract
: Introduction: Guidelines regarding antiretroviral therapy (ART) initiation in HIV infection have varied over time, with the 2015 World Health Organization recommendation suggesting ART initiation at the time of diagnosis regardless of CD4 T‐cell counts. Herein, we investigated the influence of socio‐demographic and clinical factors in addition to time trends on early ART initiation among participants of the Montreal Primary HIV Infection Study. Methods: The Montreal Primary HIV Infection Study is a prospective cohort established in three community medical centres (CMCs) and two university medical centres (UMCs). Recently diagnosed HIV‐infected adults were categorized as receiving early (vs. delayed) ART if ART was initiated within 180 days of the baseline visit. Associations between early ART initiation and socio‐demographic, socio‐economic and behavioural information were examined. Independent associations of factors linked with early ART initiation were determined using multivariable binary logistic regression analysis. Results: A total of 348 participants had a documented date of HIV acquisition of Conclusions: Early ART initiation during primary HIV infection was associated with diminished biological prognostic factors and calendar time mirroring evolution of treatment guidelines. In addition, socio‐economic factors such as having a paid employment, contribute to early ART initiation in the context of universal access to care in Canada., Introduction Human immunodeficiency virus (HIV) affects over 36.7 million people worldwide. Nearly, half of those infected with HIV remain untreated, increasing their risks for acquired immune deficiency syndrome (AIDS), onward [...]
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- 2018
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19. Restoration of Normal Interleukin-2 Production by CD4⁺ T Cells of Human Immunodeficiency Virus-Infected Patients after 9 Months of Highly Active Antiretroviral Therapy
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Weiss, Laurence, Ancuta, Petronela, Girard, Pierre-Marie, Bouhlal, Hicham, Roux, Anne, Cavaillon, Nicole Haeffner, and Kazatchkine, Michel D.
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- 1999
20. Programmed death-1-induced interleukin-10 production by monocytes impairs [CD4.sup.+] T cell activation during HIV infection
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Said, Elias A., Dupuy, Franck P., Trautmann, Lydie, Zhang, Yuwei, Shi, Yu, Far, Mohamed El-, Hill, Brenna J., Noto, Alessandra, Ancuta, Petronela, Peretz, Yoav, Fonseca, Simone G., Van Grevenynghe, Julien, Boulassel, Mohamed R., Bruneau, Julie, Shoukry, Naglaa H., Routy, Jean-Pierre, Douek, Daniel C., Haddad, Elias K., and Sekaly, Rafick-Pierre
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Interleukin-10 -- Health aspects -- Research ,Apoptosis -- Physiological aspects -- Research ,Immunologic diseases -- Risk factors -- Research ,HIV infection -- Complications and side effects -- Research ,Biological sciences ,Health - Abstract
Viral replication and microbial translocation from the gut to the blood during HIV infection lead to hyperimmune activation, which contributes to the decline in [CD4.sup.+] T cell numbers during HIV infection. Programmed death-1 (PD-1) and interleukin-10 (IL-10) are both upregulated during HIV infection. Blocking interactions between PD-1 and programmed death ligand-1 (PD-L1) and between IL-10 and IL-10 receptor (IL-10R) results in viral clearance and improves T cell function in animal models of chronic viral infections. Here we show that high amounts of microbial products and inflammatory cytokines in the plasma of HIV-infected subjects lead to upregulation of PD-1 expression on monocytes that correlates with high plasma concentrations of IL-10. Triggering of PD-1 expressed on monocytes by PD-L1 expressed on various cell types induced IL-10 production and led to reversible [CD4.sup.+] T cell dysfunction. We describe a new function for PD-1 whereby microbial products inhibit T cell expansion and function by upregulating PD-1 levels and IL-10 production by monocytes after binding of PD-1 by PD-L1., Clearance of viral infections requires potent T cell responses. However, in HIV and other chronic viral infections, viral persistence (1,2) leads to the decay of [CD4.sup.+] T cells and their [...]
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- 2010
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21. HIV reservoir size and persistence are driven by T cell survival and homeostatic proliferation
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Chomont, Nicolas, Far, Mohamed El-, Ancuta, Petronela, Trautmann, Lydie, Procopio, Francesco A., Yassine-Diab, Bader, Boucher, Genevieve, Boulassel, Mohamed-Rachid, Ghattas, Georges, Brenchley, Jason M., Schacker, Timothy W., Hill, Brenna J., Douek, Daniel C., Routy, Jean-Pierre, Haddad, Elias K., and Sekaly, Rafick-Pierre
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HIV (Viruses) -- Health aspects -- Genetic aspects -- Research -- Drug therapy ,Highly active antiretroviral therapy -- Health aspects -- Research -- Physiological aspects ,T cell proliferation -- Physiological aspects -- Research -- Genetic aspects -- Health aspects ,HIV infection -- Drug therapy -- Genetic aspects -- Research ,Biological sciences ,Health - Abstract
HIV persists in a reservoir of latently infected [CD4.sup.+] T cells in individuals treated with highly active antiretroviral therapy (HAART). Here we identify central memory ([T.sub.CM]) and transitional memory ([T.sub.TM]) [CD4.sup.+] T cells as the major cellular reservoirs for HIV and find that viral persistence is ensured by two different mechanisms. HIV primarily persists in [T.sub.CM] cells in subjects showing reconstitution of the [CD4.sup.+] compartment upon HAART. This reservoir is maintained through T cell survival and low-level antigen-driven proliferation and is slowly depleted with time. In contrast, proviral DNA is preferentially detected in [T.sub.TM] cells from aviremic individuals with low [CD4.sup.+] counts and higher amounts of interleukin-7-mediated homeostatic proliferation, a mechanism that ensures the persistence of these cells. Our results suggest that viral eradication might be achieved through the combined use of strategic interventions targeting viral replication and, as in cancer, drugs that interfere with the self renewal and persistence of proliferating memory T cells., Treatment of HIV infection has markedly reduced the death rate from AIDS and improved the quality of life of HIV-infected individuals (1). However, complete eradication of HIV with antiretroviral drugs [...]
- Published
- 2009
22. Th17 cell master transcription factor RORC2 regulates HIV-1 gene expression and viral outgrowth.
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Wiche Salinas, Tomas Raul, Yuwei Zhang, Sarnello, Daniele, Zhyvoloup, Alexander, Marchand, Laurence Raymond, Fert, Augustine, Planas, Delphine, Lodha, Manivel, Chatterjee, Debashree, Karwacz, Katarzyna, Oxenford, Sally, Routy, Jean-Pierre, Irlbeck, David, Amrine-Madsen, Heather, Ancuta, Petronela, and Fassati, Ariberto
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T helper cells ,HIV ,NUCLEAR receptors (Biochemistry) ,TRANSCRIPTION factors ,GENE expression ,COMMERCIAL products ,CURCUMIN - Abstract
Among CD4
+ T cells, T helper 17 (Th17) cells are particularly susceptible to HIV-1 infection and are depleted from mucosal sites, which causes damage to the gut barrier, resulting in a microbial translocation-induced systemic inflammation, a hallmark of disease progression. Furthermore, a proportion of latently infected Th17 cells persist long term in the gastrointestinal lymphatic tract where a low-level HIV-1 transcription is observed. This residual viremia contributes to chronic immune activation. Thus, Th17 cells are key players in HIV pathogenesis and viral persistence. It is, however, unclear why these cells are highly susceptible to HIV-1 infection. Th17 cell differentiation depends on the expression of the master transcriptional regulator RORC2, a retinoic acid-related nuclear hormone receptor that regulates specific transcriptional programs by binding to promoter/enhancer DNA. Here, we report that RORC2 is a key host cofactor for HIV replication in Th17 cells. We found that specific inhibitors that bind to the RORC2 ligand-binding domain reduced HIV replication in CD4+ T cells. The depletion of RORC2 inhibited HIV-1 infection, whereas its overexpression enhanced it. RORC2 was also found to promote HIV-1 gene expression by binding to the nuclear receptor responsive element in the HIV-1 long terminal repeats (LTR). In treated HIV-1 patients, RORC2+ CD4 T cells contained more proviral DNA than RORC2− cells. Pharmacological inhibition of RORC2 potently reduced HIV-1 outgrowth in CD4+ T cells from antiretroviral-treated patients. Altogether, these results provide an explanation as to why Th17 cells are highly susceptible to HIV-1 infection and suggest that RORC2 may be a cell-specific target for HIV-1 therapy. [ABSTRACT FROM AUTHOR]- Published
- 2021
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23. Overt IL-32 isoform expression at intestinal level during HIV-1 infection is negatively regulated by IL-17A.
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Moreira Gabriel, Etiene, Wiche Salinas, Tomas Raul, Gosselin, Annie, Larouche-Anctil, Etienne, Durand, Madeleine, Landay, Alan L., El-Far, Mohamed, Tremblay, Cécile L., Routy, Jean-Pierre, and Ancuta, Petronela
- Published
- 2021
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24. Subclinical Carotid Artery Atherosclerosis Is Associated With Increased Expression of Peripheral Blood IL-32 Isoforms Among Women Living With HIV.
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El-Far, Mohamed, Hanna, David B., Durand, Madeleine, Larouche-Anctil, Etienne, Sylla, Mohamed, Chartrand-Lefebvre, Carl, Cloutier, Guy, Goulet, Jean Philippe, Kassaye, Seble, Karim, Roksana, Kizer, Jorge R., French, Audrey L., Gange, Stephen J., Lazar, Jason M., Hodis, Howard N., Routy, Jean-Pierre, Ancuta, Petronela, Chomont, Nicolas, Landay, Alan L., and Kaplan, Robert C.
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- 2021
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25. Mechanisms of HIV-1 Neurotropism
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Dunfee, Rebecca, Thomas, Elaine R., Gorry, Paul R., Wang, Jianbin, Ancuta, Petronela, and Gabuzda, Dana
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- 2006
26. Transendothelial migration of CD16 + monocytes in response to fractalkine under constitutive and inflammatory conditions
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Ancuta, Petronela, Moses, Ashlee, and Gabuzda, Dana
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- 2004
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27. A Tale of Two Viruses: Immunological Insights Into HCV/HIV Coinfection.
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Gobran, Samaa T., Ancuta, Petronela, and Shoukry, Naglaa H.
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FIBROSIS ,HIV ,MIXED infections ,HIV-positive persons ,MEN who have sex with men ,VIRAL load - Abstract
Nearly 2.3 million individuals worldwide are coinfected with human immunodeficiency virus (HIV) and hepatitis C virus (HCV). Odds of HCV infection are six times higher in people living with HIV (PLWH) compared to their HIV-negative counterparts, with the highest prevalence among people who inject drugs (PWID) and men who have sex with men (MSM). HIV coinfection has a detrimental impact on the natural history of HCV, including higher rates of HCV persistence following acute infection, higher viral loads, and accelerated progression of liver fibrosis and development of end-stage liver disease compared to HCV monoinfection. Similarly, it has been reported that HCV coinfection impacts HIV disease progression in PLWH receiving anti-retroviral therapies (ART) where HCV coinfection negatively affects the homeostasis of CD4
+ T cell counts and facilitates HIV replication and viral reservoir persistence. While ART does not cure HIV, direct acting antivirals (DAA) can now achieve HCV cure in nearly 95% of coinfected individuals. However, little is known about how HCV cure and the subsequent resolution of liver inflammation influence systemic immune activation, immune reconstitution and the latent HIV reservoir. In this review, we will summarize the current knowledge regarding the pathogenesis of HIV/HCV coinfection, the effects of HCV coinfection on HIV disease progression in the context of ART, the impact of HIV on HCV-associated liver morbidity, and the consequences of DAA-mediated HCV cure on immune reconstitution and HIV reservoir persistence in coinfected patients. [ABSTRACT FROM AUTHOR]- Published
- 2021
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28. Antiretroviral Drug Transporters and Metabolic Enzymes in Circulating Monocytes and Monocyte-Derived Macrophages of ART-Treated People Living With HIV and HIV-Uninfected Individuals.
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Hoque, Tozammel M. D., Cattin, Amélie, Whyte-Allman, Sana-Kay, Winchester, Lee, Fletcher, Courtney V., Routy, Jean-Pierre, Ancuta, Petronela, and Bendayan, Reina
- Published
- 2021
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29. Loss of CD96 Expression as a Marker of HIV-Specific CD8+ T-Cell Differentiation and Dysfunction.
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Bunet, Rémi, Nayrac, Manon, Ramani, Hardik, Sylla, Mohamed, Durand, Madeleine, Chartrand-Lefebvre, Carl, Routy, Jean-Pierre, Landay, Alan L., Gauchat, Jean-Francois, Chomont, Nicolas, Ancuta, Petronela, Kaufmann, Daniel E., Bernard, Nicole, Tremblay, Cécile L., and El-Far, Mohamed
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HIV-positive persons ,PREMATURE aging (Medicine) ,IMMUNOGLOBULIN receptors ,IMMUNOSENESCENCE ,T cells ,PSYCHONEUROIMMUNOLOGY - Abstract
Persistent immune activation and inflammation in people living with HIV (PLWH) are associated with immunosenescence, premature aging and increased risk of non-AIDS comorbidities, with the underlying mechanisms not fully understood. In this study, we show that downregulation of the T-cell immunoglobulin receptor CD96 on CD8
+ T cells from PLWH is associated with decreased expression of the co-stimulatory receptors CD27 and CD28, higher expression of the senescence marker CD57 and accumulation of a terminally differentiated T-cell memory phenotype. In addition, we show that CD96-low CD8+ T-cells display lower proliferative potential compared to their CD96-high counterparts and that loss of CD96 expression by HIV-specific CD8+ T-cells is associated with a suboptimal response to HIV antigens. In conclusion, our results suggest that CD96 marks CD8+ T-cells with competent responses to HIV and the loss of its expression might be used as a biomarker for CD8+ T-cell senescence and dysfunction in PLWH. [ABSTRACT FROM AUTHOR]- Published
- 2021
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30. Transcriptional profiling reveals developmental relationship and distinct biological functions of CD16+ and CD16- monocyte subsets
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Zhou Xiaobo, Gosselin Annie, Wacleche Vanessa, Misra Vikas, Liu Kuang-Yu, Ancuta Petronela, and Gabuzda Dana
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Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Human peripheral blood monocytes (Mo) consist of subsets distinguished by expression of CD16 (FCγRIII) and chemokine receptors. Classical CD16- Mo express CCR2 and migrate in response to CCL2, while a minor CD16+ Mo subset expresses CD16 and CX3CR1 and migrates into tissues expressing CX3CL1. CD16+ Mo produce pro-inflammatory cytokines and are expanded in certain inflammatory conditions including sepsis and HIV infection. Results To gain insight into the developmental relationship and functions of CD16+ and CD16- Mo, we examined transcriptional profiles of these Mo subsets in peripheral blood from healthy individuals. Of 16,328 expressed genes, 2,759 genes were differentially expressed and 228 and 250 were >2-fold upregulated and downregulated, respectively, in CD16+ compared to CD16- Mo. CD16+ Mo were distinguished by upregulation of transcripts for dendritic cell (DC) (SIGLEC10, CD43, RARA) and macrophage (MΦ) (CSF1R/CD115, MafB, CD97, C3aR) markers together with transcripts relevant for DC-T cell interaction (CXCL16, ICAM-2, LFA-1), cell activation (LTB, TNFRSF8, LST1, IFITM1-3, HMOX1, SOD-1, WARS, MGLL), and negative regulation of the cell cycle (CDKN1C, MTSS1), whereas CD16- Mo were distinguished by upregulation of transcripts for myeloid (CD14, MNDA, TREM1, CD1d, C1qR/CD93) and granulocyte markers (FPR1, GCSFR/CD114, S100A8-9/12). Differential expression of CSF1R, CSF3R, C1QR1, C3AR1, CD1d, CD43, CXCL16, and CX3CR1 was confirmed by flow cytometry. Furthermore, increased expression of RARA and KLF2 transcripts in CD16+ Mo coincided with absence of cell surface cutaneous lymphocyte associated antigen (CLA) expression, indicating potential imprinting for non-skin homing. Conclusion These results suggest that CD16+ and CD16- Mo originate from a common myeloid precursor, with CD16+ Mo having a more MΦ – and DC-like transcription program suggesting a more advanced stage of differentiation. Distinct transcriptional programs, together with their recruitment into tissues via different mechanisms, also suggest that CD16+ and CD16- Mo give rise to functionally distinct DC and MΦ in vivo.
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- 2009
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31. Cytomegalovirus Seropositivity Is Associated With Increased Microbial Translocation in People Living With Human Immunodeficiency Virus and Uninfected Controls.
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Ramendra, Rayoun, Isnard, Stéphane, Lin, John, Fombuena, Brandon, Ouyang, Jing, Mehraj, Vikram, Zhang, Yonglong, Finkelman, Malcolm, Costiniuk, Cecilia, Lebouché, Bertrand, Chartrand-Lefebvre, Carl, Durand, Madeleine, Tremblay, Cécile, Ancuta, Petronela, Boivin, Guy, and Routy, Jean-Pierre
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BACTERIAL physiology ,BIOMARKERS ,CYTOKINES ,CYTOMEGALOVIRUS diseases ,EPSTEIN-Barr virus diseases ,HIV-positive persons ,IMMUNOGLOBULINS ,SOCIOECONOMIC factors ,HIV seroconversion ,DISEASE progression ,SYSTEMIC inflammatory response syndrome - Abstract
Background Cytomegalovirus (CMV) seropositivity and anti-CMV immunoglobulin G (IgG) levels are associated with adverse health outcomes in elderly populations. Among people living with human immunodeficiency virus (PLWH), CMV seropositivity has been associated with persistent CD8 T-cell elevation and increased risk of developing non-AIDS comorbidities despite long-term antiretroviral therapy (ART). Herein, we investigated whether CMV seropositivity and elevation of anti-CMV IgG levels were associated with increased epithelial gut damage, microbial translocation, and systemic inflammation. Methods A total of 150 PLWH (79 ART-naive and 71 ART-treated) were compared to 26 without human immunodeficiency virus (HIV) infection (uninfected controls). Plasma markers of HIV disease progression, epithelial gut damage, microbial translocation, nonspecific B-cell activation, anti-CMV and anti–Epstein-Barr virus (EBV) IgG levels, and proinflammatory cytokines were measured. Results CMV seropositivity and elevated anti-CMV IgG levels were associated with markers of epithelial gut damage, microbial translocation, and inflammation in PLWH and participants without HIV infection. In contrast, total nonspecific IgG, immunoglobulin M, immunoglobulin A, and anti-EBV IgG levels were not associated with these markers. CMV seropositivity was associated with markers of epithelial gut damage, microbial translocation, and inflammation independent of sociodemographic and behavioral characteristics of the study population. Conclusions CMV-seropositive people with and without HIV had increased epithelial gut damage, microbial translocation, and inflammation. Furthermore, anti-CMV IgG levels were independently associated with increased epithelial gut damage and microbial translocation. CMV coinfection may partially explain persistent gut damage, microbial translocation, and inflammation in ART-treated PLWH. [ABSTRACT FROM AUTHOR]
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- 2020
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32. Repurposing Metformin in Nondiabetic People With HIV: Influence on Weight and Gut Microbiota.
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Isnard, Stéphane, Lin, John, Fombuena, Brandon, Ouyang, Jing, Varin, Thibault V, Richard, Corentin, Marette, André, Ramendra, Rayoun, Planas, Delphine, Marchand, Laurence Raymond, Messaoudene, Meriem, Ley, Claude P Van der, Kema, Ido P, Ahmed, Darakhshan Sohail, Zhang, Yonglong, Finkelman, Malcolm, Routy, Bertrand, Angel, Jonathan, Ancuta, Petronela, and Routy, Jean-Pierre
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GUT microbiome ,METFORMIN ,POLYCYSTIC ovary syndrome ,DYSLIPIDEMIA ,HIV ,CLINICAL trials - Abstract
Background People with HIV (PWH) taking antiretroviral therapy (ART) may experience weight gain, dyslipidemia, increased risk of non-AIDS comorbidities, and long-term alteration of the gut microbiota. Both low CD4/CD8 ratio and chronic inflammation have been associated with changes in the gut microbiota of PWH. The antidiabetic drug metformin has been shown to improve gut microbiota composition while decreasing weight and inflammation in diabetes and polycystic ovary syndrome. Nevertheless, it remains unknown whether metformin may benefit PWH receiving ART, especially those with a low CD4/CD8 ratio. Methods In the Lilac pilot trial, we recruited 23 nondiabetic PWH receiving ART for more than 2 years with a low CD4/CD8 ratio (<0.7). Blood and stool samples were collected during study visits at baseline, after a 12-week metformin treatment, and 12 weeks after discontinuation. Microbiota composition was analyzed by 16S rDNA gene sequencing, and markers of inflammation were assessed in plasma. Results Metformin decreased weight in PWH, and weight loss was inversely correlated with plasma levels of the satiety factor GDF-15. Furthermore, metformin changed the gut microbiota composition by increasing the abundance of anti-inflammatory bacteria such as butyrate-producing species and the protective Akkermansia muciniphila. Conclusions Our study provides the first evidence that a 12-week metformin treatment decreased weight and favored anti-inflammatory bacteria abundance in the microbiota of nondiabetic ART-treated PWH. Larger randomized placebo-controlled clinical trials with longer metformin treatment will be needed to further investigate the role of metformin in reducing inflammation and the risk of non-AIDS comorbidities in ART-treated PWH. [ABSTRACT FROM AUTHOR]
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- 2020
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33. Improving HIV Outgrowth by Optimizing Cell-Culture Conditions and Supplementing With all-trans Retinoic Acid.
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Zhang, Yuwei, Planas, Delphine, Raymond Marchand, Laurence, Massanella, Marta, Chen, Huicheng, Wacleche, Vanessa Sue, Gosselin, Annie, Goulet, Jean-Philippe, Filion, Mario, Routy, Jean-Pierre, Chomont, Nicolas, and Ancuta, Petronela
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TRETINOIN ,ANTIRETROVIRAL agents ,FLOW cytometry ,T cells ,TREATMENT effectiveness - Abstract
The persistence of replication-competent HIV reservoirs in people living with HIV (PLWH) receiving antiretroviral therapy (ART) is a barrier to cure. Therefore, their accurate quantification is essential for evaluating the efficacy of new therapeutic interventions and orienting the decision to interrupt ART. Quantitative viral outgrowth assays (QVOAs) represent the " gold standard " for measuring the size of replication-competent HIV reservoirs. However, they require large numbers of cells and are technically challenging. This justifies the need for the development of novel simplified methods adapted for small biological samples. Herein, we sought to simplify the viral outgrowth procedure (VOP) by (i) using memory CD4
+ T-cells, documented to be enriched in HIV reservoirs (ii) optimizing cell-culture conditions, and (iii) supplementing with a ll-trans retinoic acid (ATRA), a positive regulator of HIV replication. Memory CD4+ T-cells were sorted from the peripheral blood of ART-treated (HIV+ART; n = 14) and untreated (HIV+; n = 5) PLWH. The VOP was first performed with one original replicate of 1 × 106 cells/well in 48-well plates. Cells were stimulated via CD3/CD28 for 3 days, washed to remove residual CD3/CD28 Abs, split every 3 days for optimal cell density, and cultured in the presence or the absence of ATRA for 12 days. Soluble and intracellular HIV-p24 levels were quantified by ELISA and flow cytometry, respectively. Optimal cell-culture density achieved by splitting improved HIV outgrowth detection. ATRA promoted superior/accelerated detection of replication-competent HIV in all HIV+ART individuals tested, including those with low/undetectable viral outgrowth in the absence of ATRA. Finally, this VOP was used to design a simplified ATRA-based QVOA by including 4 and 6 original replicates of 1 × 106 cells/well in 48-well plates and 2 × 105 cells/well in 96-well plates, respectively. Consistently, the number of infectious units per million cells (IUPM) was significantly increased in the presence of ATRA. In conclusion, we demonstrate that memory CD4+ T-cell splitting for optimal density in culture and ATRA supplementation significantly improved the efficacy of HIV outgrowth in a simplified ATRA-based QVOA performed in the absence of feeder/target cells or indicator cell lines. [ABSTRACT FROM AUTHOR]- Published
- 2020
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34. Circulating (1→3)-β-D-glucan Is Associated With Immune Activation During Human Immunodeficiency Virus Infection.
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Mehraj, Vikram, Ramendra, Rayoun, Isnard, Stéphane, Dupuy, Franck P, Ponte, Rosalie, Chen, Jun, Kema, Ido, Jenabian, Mohammad-Ali, Costinuik, Cecilia T, Lebouché, Bertrand, Thomas, Réjean, Coté, Pierre, Leblanc, Roger, Baril, Jean-Guy, Durand, Madeleine, Chartrand-Lefebvre, Carl, Tremblay, Cécile, Ancuta, Petronela, Bernard, Nicole F, and Sheppard, Donald C
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BACTERIAL physiology ,GENE expression ,GLUCANS ,HIV ,HIV infections ,LONGITUDINAL method ,MONOCYTES ,T cells ,VIRAL load ,ANTIRETROVIRAL agents ,HIGHLY active antiretroviral therapy ,CROSS-sectional method ,LIPOPOLYSACCHARIDES - Abstract
Background Microbial translocation from the gut to systemic circulation contributes to immune activation during human immunodeficiency virus (HIV) infection and is usually assessed by measuring plasma levels of bacterial lipopolysaccharide (LPS). Fungal colonization in the gut increases during HIV-infection and people living with HIV (PLWH) have increased plasma levels of fungal polysaccharide (1→3)-β-D-Glucan (βDG). We assessed the contribution of circulating DG to systemic immune activation in PLWH. Methods Cross-sectional and longitudinal assessments of plasma βDG levels were conducted along with markers of HIV disease progression, epithelial gut damage, bacterial translocation, proinflammatory cytokines, and βDG-specific receptor expression on monocytes and natural killer (NK) cells. Results Plasma βDG levels were elevated during early and chronic HIV infection and persisted despite long-term antiretroviral therapy (ART). βDG increased over 24 months without ART but remained unchanged after 24 months of treatment. βDG correlated negatively with CD4 T-cell count and positively with time to ART initiation, viral load, intestinal fatty acid–binding protein, LPS, and soluble LPS receptor soluble CD14 (sCD14). Elevated βDG correlated positively with indoleamine-2,3-dioxygenase-1 enzyme activity, regulatory T-cell frequency, activated CD38
+ Human Leukocyte Antigen - DR isotype (HLA-DR)+ CD4 and CD8 T cells and negatively with Dectin-1 and NKp30 expression on monocytes and NK cells, respectively. Conclusions PLWH have elevated plasma βDG in correlation with markers of disease progression, gut damage, bacterial translocation, and inflammation. Early ART initiation prevents further βDG increase. This fungal antigen contributes to immune activation and represents a potential therapeutic target to prevent non–acquired immunodeficiency syndrome events. [ABSTRACT FROM AUTHOR]- Published
- 2020
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35. Plasma Levels of C-Type Lectin REG3α and Gut Damage in People With Human Immunodeficiency Virus.
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Isnard, Stéphane, Ramendra, Rayoun, Dupuy, Franck P, Lin, John, Fombuena, Brandon, Kokinov, Nikola, Kema, Ido, Jenabian, Mohammad-Ali, Lebouché, Bertrand, Costiniuk, Cecilia T, Ancuta, Petronela, Bernard, Nicole F, Silverman, Michael S, Lakatos, Peter L, Durand, Madeleine, Tremblay, Cécile, Routy, Jean-Pierre, Study, Montreal Primary HIV Infection, Montreal primary HIV infection study, and Canadian cohort of HIV+ slow progressors
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LECTINS ,HIV ,INFLAMMATORY bowel diseases ,VIRAL load ,BIOMARKERS ,ANTIRETROVIRAL agents - Abstract
Background: Regenerating islet-derived protein 3α (REG3α) is an antimicrobial peptide secreted by intestinal Paneth cells. Circulating REG3α has been identified as a gut damage marker in inflammatory bowel diseases. People living with human immunodeficiency virus (PWH) on antiretroviral therapy (ART) present with an abnormal intestinal landscape leading to microbial translocation, persistent inflammation, and development of non-AIDS comorbidities. Herein, we assessed REG3α as a marker of gut damage in PWH.Methods: Plasma from 169 adult PWH, including 30 elite controllers (ECs), and 30 human immunodeficiency virus (HIV)-uninfected controls were assessed. REG3α plasma levels were compared with HIV disease progression, epithelial gut damage, microbial translocation, and immune activation markers.Results: Cross-sectionally, REG3α levels were elevated in untreated and ART-treated PWH compared with controls. ECs also had elevated REG3α levels compared to controls. Longitudinally, REG3α levels increased in PWH without ART and decreased in those who initiated ART. REG3α levels were inversely associated with CD4 T-cell count and CD4:CD8 ratio, while positively correlated with HIV viral load in untreated participants, and with fungal product translocation and inflammatory markers in all PWH.Conclusions: Plasma REG3α levels were elevated in PWH, including ECs. The gut inflammatory marker REG3α may be used to evaluate therapeutic interventions and predict non-AIDS comorbidity risks in PWH. [ABSTRACT FROM AUTHOR]- Published
- 2020
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36. HIV-1 is rarely detected in blood and colon myeloid cells during viral-suppressive antiretroviral therapy.
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Cattin, Amélie, Wiche Salinas, Tomas Raul, Gosselin, Annie, Planas, Delphine, Shacklett, Barbara, Cohen, Eric A., Ghali, Maged P., Routy, Jean-Pierre, and Ancuta, Petronela
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- 2019
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37. Effect of metformin on the size of the HIV reservoir in non-diabetic ART-treated individuals: single-arm non-randomised Lilac pilot study protocol.
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Routy, Jean-Pierre, Isnard, Stéphane, Mehraj, Vikram, Ostrowski, Mario, Chomont, Nicolas, Ancuta, Petronela, Ponte, Rosalie, Planas, Delphine, Dupuy, Franck P., and Angel, Jonathan B.
- Abstract
Introduction People living with HIV (PLWH) on antiretroviral therapy (ART) do not progress to AIDS. However, they still suffer from an increased risk of inflammation-associated complications. HIV persists in long-lived CD4+ T cells, which form the major viral reservoir. The persistence of this reservoir despite long-term ART is the major hurdle to curing HIV. Importantly, the size of the HIV reservoir is larger in individuals who start ART late in the course of infection and have a low CD4+/CD8+ ratio. HIV reservoir size is also linked to the levels of persistent inflammation on ART. Thus, novel strategies to reduce immune inflammation and improve the host response to control the HIV reservoir would be a valuable addition to current ART. Among the different strategies under investigation is metformin, a widely used antidiabetic drug that was recently shown to modulate T-cell activation and inflammation. Treatment of non-diabetic individuals with metformin controls inflammation by improving glucose metabolism and by regulating intracellular immunometabolic checkpoints such as the adenosin 5 monophosphate activated protein kinase and mammalian target of rapamycin, in association with microbiota modification. Methods and analysis 22 PLWH on ART for more than 3 years, at high risk of inflammation or the development of non-AIDS events (low CD4+/CD8+ ratio) will be recruited in a clinical single-arm pilot study. We will test whether supplementing ART with metformin in non-diabetic HIV-infected individuals can reduce the size of the HIV reservoir as determined by various virological assays. The expected outcome of this study is a reduction in both the size of the HIV reservoir and inflammation following the addition of metformin to ART, thus paving the way towards HIV eradication. Ethics and dissemination Ethical approval: McGill university Health Centre committee number MP-37-2016-2456. Canadian Canadian Institutes of Health Research/Canadian HIV Trials Network (CTN) protocol CTNPT027. Results will be made available through publication in peer-reviewed journals and through the CTN website. Trial registration number NCT02659306 [ABSTRACT FROM AUTHOR]
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- 2019
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38. HIV persistence in mucosal CD4+ T cells within the lungs of adults receiving long-term suppressive antiretroviral therapy.
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Costiniuk, Cecilia T., Salahuddin, Syim, Farnos, Omar, Olivenstein, Ron, Pagliuzza, Amélie, Orlova, Marianna, Schurr, Erwin, De Castro, Christina, Bourbeau, Jean, Routy, Jean-Pierre, Ancuta, Petronela, Chomont, Nicolas, and Jenabian, Mohammad-Ali
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- 2018
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39. Targeting the interleukin‐17 pathway to prevent acute respiratory distress syndrome associated with SARS‐CoV‐2 infection.
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Wiche Salinas, Tomas Raul, Zheng, Boyang, Routy, Jean‐Pierre, and Ancuta, Petronela
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ADULT respiratory distress syndrome ,COVID-19 ,MERS coronavirus ,INFECTION ,BRONCHIOLITIS ,INTERLEUKIN-17 - Abstract
Targeting the interleukin-17 pathway to prevent acute respiratory distress syndrome associated with SARS-CoV-2 infection Keywords: acute respiratory distress syndrome; COVID-19; cytokine EN acute respiratory distress syndrome COVID-19 cytokine 797 799 3 07/17/20 20200801 NES 200801 The novel coronavirus causing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for coronavirus disease 2019 (COVID-19) pandemic. Risk factors associated with acute respiratory distress syndrome and death in patients with coronavirus disease 2019 pneumonia in Wuhan, China. Cell host response to infection with novel human coronavirus EMC predicts potential antivirals and important differences with SARS coronavirus. [Extracted from the article]
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- 2020
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40. Restoring Inflammatory Mediator Balance after Sofosbuvir-Induced Viral Clearance in Patients with Chronic Hepatitis C.
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Saraiva, Geórgia Nascimento, Rosário, Natalia Fonseca do, Medeiros, Thalia, Leite, Paulo Emílio Côrrea, Lacerda, Gilmar de Souza, Andrade, Thaís Guaraná de, de Azeredo, Elzinandes Leal, Ancuta, Petronela, Almeida, Jorge Reis, Xavier, Analúcia Rampazzo, and Silva, Andrea Alice
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INFLAMMATION ,SOFOSBUVIR ,HEPATITIS C virus ,ANTIVIRAL agents ,INTERFERONS - Abstract
This study aimed at analyzing circulating levels of inflammatory and profibrogenic cytokines in patients with hepatitis C virus (HCV) chronic infection undergoing therapy with direct-acting antiviral agents (DAA) and correlating these immune biomarkers with liver disease status. We studied 88 Brazilian monoinfected chronic hepatitis C patients receiving interferon- (IFN-) free sofosbuvir-based regimens for 12 or 24 weeks, followed-up before therapy initiation and three months after the end of treatment. Liver disease was determined by transient elastography, in addition to APRI and FIB-4 indexes. Analysis of 30 immune mediators was carried out by multiplex or enzymatic immunoassays. Sustained virological response rate was 98.9%. Serum levels of cytokines were increased in HCV-infected patients when compared to control group. CCL-2, CCL-3, CCL-4, CXCL-8, CXCL-10, IL-1β, IL-15, IFN-γ, IL-4, IL-10, TGF-β, FGFb, and PAI-1 decreased significantly after antiviral therapy, reaching values similar to noninfected controls. TGF-β and suPAR levels were associated with fibrosis/cirrhosis. Also, we observed amelioration in hepatic parameters after DAA treatment. Together, our results suggest that viral control induced by IFN-free DAA therapy restores inflammatory mediators in association with improvement in liver function. [ABSTRACT FROM AUTHOR]
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- 2018
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41. The Biology of Monocytes and Dendritic Cells: Contribution to HIV Pathogenesis.
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Wacleche, Vanessa Sue, Tremblay, Cécile L., Routy, Jean-Pierre, and Ancuta, Petronela
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MONOCYTES ,HIGHLY active antiretroviral therapy ,HIV ,DENDRITIC cells ,MACROPHAGES ,HOMEOSTASIS - Abstract
Myeloid cells such as monocytes, dendritic cells (DC) and macrophages (MF) are key components of the innate immune system contributing to the maintenance of tissue homeostasis and the development/resolution of immune responses to pathogens. Monocytes and DC, circulating in the blood or infiltrating various lymphoid and non-lymphoid tissues, are derived from distinct bone marrow precursors and are typically short lived. Conversely, recent studies revealed that subsets of tissue resident MF are long-lived as they originate from embryonic/fetal precursors that have the ability to self-renew during the life of an individual. Pathogens such as the human immunodeficiency virus type 1 (HIV-1) highjack the functions of myeloid cells for viral replication (e.g., MF) or distal dissemination and cell-to-cell transmission (e.g., DC). Although the long-term persistence of HIV reservoirs in CD4+ T-cells during viral suppressive antiretroviral therapy (ART) is well documented, the ability of myeloid cells to harbor replication competent viral reservoirs is still a matter of debate. This review summarizes the current knowledge on the biology of monocytes and DC during homeostasis and in the context of HIV-1 infection and highlights the importance of future studies on long-lived resident MF to HIV persistence in ART-treated patients. [ABSTRACT FROM AUTHOR]
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- 2018
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42. Host MicroRNAs-221 and -222 Inhibit HIV-1 Entry in Macrophages by Targeting the CD4 Viral Receptor.
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Lodge, Robert, Ferreira Barbosa, Jérémy A., Lombard-Vadnais, Félix, Gilmore, Julian C., Deshiere, Alexandre, Gosselin, Annie, Wiche Salinas, Tomas Raul, Bego, Mariana G., Power, Christopher, Routy, Jean-Pierre, Ancuta, Petronela, Tremblay, Michel J., and Cohen, Éric A.
- Abstract
Summary Macrophages are heterogeneous immune cells with distinct origins, phenotypes, functions, and tissue localization. Their susceptibility to HIV-1 is subject to variations from permissiveness to resistance, owing in part to regulatory microRNAs. Here, we used RNA sequencing (RNA-seq) to examine the expression of >400 microRNAs in productively infected and bystander cells of HIV-1-exposed macrophage cultures. Two microRNAs upregulated in bystander macrophages, miR-221 and miR-222, were identified as negative regulators of CD4 expression and CD4-mediated HIV-1 entry. Both microRNAs were enhanced by tumor necrosis factor alpha (TNF-α), an inhibitor of CD4 expression. MiR-221/miR-222 inhibitors recovered HIV-1 entry in TNF-α-treated macrophages by enhancing CD4 expression and increased HIV-1 replication and spread in macrophages by countering TNF-α-enhanced miR-221/miR-222 expression in bystander cells. In line with these findings, HIV-1-resistant intestinal myeloid cells express higher levels of miR-221 than peripheral blood monocytes. Thus, miR-221/miR-222 act as effectors of the antiviral host response activated during macrophage infection that restrict HIV-1 entry. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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43. The Th17 Lineage: From Barrier Surfaces Homeostasis to Autoimmunity, Cancer, and HIV-1 Pathogenesis.
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Wacleche, Vanessa Sue, Landay, Alan, Routy, Jean-Pierre, and Ancuta, Petronela
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T helper cells ,HOMEOSTASIS ,AUTOIMMUNITY ,CARCINOGENESIS ,HIV ,ANTIRETROVIRAL agents - Abstract
The T helper 17 (Th17) cells represent a subset of CD4+ T-cells with unique effector functions, developmental plasticity, and stem-cell features. Th17 cells bridge innate and adaptive immunity against fungal and bacterial infections at skin and mucosal barrier surfaces. Although Th17 cells have been extensively studied in the context of autoimmunity, their role in various other pathologies is underexplored and remains an area of open investigation. This review summarizes the history of Th17 cell discovery and the current knowledge relative to the beneficial role of Th17 cells in maintaining mucosal immunity homeostasis. We further discuss the concept of Th17 pathogenicity in the context of autoimmunity, cancer, and HIV infection, and we review the most recent discoveries on molecular mechanisms regulating HIV replication/persistence in pathogenic Th17 cells. Finally, we stress the need for novel fundamental research discovery-based Th17-specific therapeutic interventions to treat pathogenic conditions associated with Th17 abnormalities, including HIV infection. [ABSTRACT FROM AUTHOR]
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- 2017
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44. The Canadian HIV and aging cohort study - determinants of increased risk of cardio-vascular diseases in HIV-infected individuals: rationale and study protocol.
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Durand, Madeleine, Chartrand-Lefebvre, Carl, Baril, Jean-Guy, Trottier, Sylvie, Trottier, Benoit, Harris, Marianne, Walmsley, Sharon, Conway, Brian, Wong, Alexander, Routy, Jean-Pierre, Kovacs, Colin, MacPherson, Paul A., Monteith, Kenneth Marc, Mansour, Samer, Thanassoulis, George, Abrahamowicz, Michal, Zhitong Zhu, Tsoukas, Christos, Ancuta, Petronela, and Bernard, Nicole
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CARDIOVASCULAR diseases risk factors ,HIV-positive persons ,ANTIRETROVIRAL agents ,PATHOLOGICAL physiology ,AGE factors in disease ,PUBLIC health ,HEALTH ,HIV infection complications ,TYPE 2 diabetes complications ,HIV infection epidemiology ,AGING ,CARDIOVASCULAR diseases ,CHRONIC diseases ,COMPARATIVE studies ,INFLAMMATION ,LONGITUDINAL method ,RESEARCH methodology ,MEDICAL cooperation ,MYOCARDIAL infarction ,TYPE 2 diabetes ,RESEARCH ,RESEARCH funding ,STROKE ,COMORBIDITY ,EVALUATION research ,DISEASE incidence - Abstract
Background: With potent antiretroviral drugs, HIV infection is becoming a chronic disease. Emergence of comorbidities, particularly cardiovascular disease (CVD) has become a leading concern for patients living with the infection. We hypothesized that the chronic and persistent inflammation and immune activation associated with HIV disease leads to accelerated aging, characterized by CVD. This will translate into higher incidence rates of CVD in HIV infected participants, when compared to HIV negative participants, after adjustment for traditional CVD risk factors. When characterized further using cardiovascular imaging, biomarkers, immunological and genetic profiles, CVD associated with HIV will show different characteristics compared to CVD in HIV-negative individuals.Methods/design: The Canadian HIV and Aging cohort is a prospective, controlled cohort study funded by the Canadian Institutes of Health Research. It will recruit patients living with HIV who are aged 40 years or older or have lived with HIV for 15 years or more. A control population, frequency matched for age, sex, and smoking status, will be recruited from the general population. Patients will attend study visits at baseline, year 1, 2, 5 and 8. At each study visit, data on complete medical and pharmaceutical history will be captured, along with anthropometric measures, a complete physical examination, routine blood tests and electrocardiogram. Consenting participants will also contribute blood samples to a research biobank. The primary outcome is incidence of a composite of: myocardial infarction, coronary revascularization, stroke, hospitalization for angina or congestive heart failure, revascularization or amputation for peripheral artery disease, or cardiovascular death. Preplanned secondary outcomes are all-cause mortality, incidence of the metabolic syndrome, incidence of type 2 diabetes, incidence of renal failure, incidence of abnormal bone mineral density and body fat distribution. Patients participating to the cohort will be eligible to be enrolled in four pre-planned sub-studies of cardiovascular imaging, glucose metabolism, immunological and genetic risk profile.Discussion: The Canadian HIV and Aging Cohort will provide insights on pathophysiological pathways leading to premature CVD for patients living with HIV. [ABSTRACT FROM AUTHOR]- Published
- 2017
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45. Digoxin reveals a functional connection between HIV-1 integration preference and T-cell activation.
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Zhyvoloup, Alexander, Melamed, Anat, Anderson, Ian, Planas, Delphine, Lee, Chen-Hsuin, Kriston-Vizi, Janos, Ketteler, Robin, Merritt, Andy, Routy, Jean-Pierre, Ancuta, Petronela, Bangham, Charles R. M., and Fassati, Ariberto
- Subjects
DIGOXIN ,HIV infections ,T cells ,POINT mutation (Biology) ,GENE expression ,CELL metabolism - Abstract
HIV-1 integrates more frequently into transcribed genes, however the biological significance of HIV-1 integration targeting has remained elusive. Using a selective high-throughput chemical screen, we discovered that the cardiac glycoside digoxin inhibits wild-type HIV-1 infection more potently than HIV-1 bearing a single point mutation (N74D) in the capsid protein. We confirmed that digoxin repressed viral gene expression by targeting the cellular Na
+ /K+ ATPase, but this did not explain its selectivity. Parallel RNAseq and integration mapping in infected cells demonstrated that digoxin inhibited expression of genes involved in T-cell activation and cell metabolism. Analysis of >400,000 unique integration sites showed that WT virus integrated more frequently than N74D mutant within or near genes susceptible to repression by digoxin and involved in T-cell activation and cell metabolism. Two main gene networks down-regulated by the drug were CD40L and CD38. Blocking CD40L by neutralizing antibodies selectively inhibited WT virus infection, phenocopying digoxin. Thus the selectivity of digoxin depends on a combination of integration targeting and repression of specific gene networks. The drug unmasked a functional connection between HIV-1 integration and T-cell activation. Our results suggest that HIV-1 evolved integration site selection to couple its early gene expression with the status of target CD4+ T-cells, which may affect latency and viral reactivation. [ABSTRACT FROM AUTHOR]- Published
- 2017
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46. HIV persists in CCR6+CD4+ T cells from colon and blood during antiretroviral therapy.
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Gosselin, Annie, Wiche Salinas, Tomas Raul, Planas, Delphine, Wacleche, Vanessa S., Yuwei Zhang, Fromentin, Rémi, Chomont, Nicolas, Cohen, Éric A., Shacklett, Barbara, Mehraj, Vikram, Ghali, Maged P., Routy, Jean-Pierre, Ancuta, Petronela, and Zhang, Yuwei
- Published
- 2017
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47. Immune tolerance properties of the testicular tissue as a viral sanctuary site in ART-treated HIV-infected adults.
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Jenabian, Mohammad-Ali, Costiniuk, Cecilia T., Mehraj, Vikram, Ghazawi, Feras M., Fromentin, Rémi, Brousseau, Joëlle, Brassard, Pierre, Bélanger, Maud, Ancuta, Petronela, Bendayan, Reina, Chomont, Nicolas, Routy, Jean-Pierre, and Orchid study group
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- 2016
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48. ION CANTACUZINO (JEAN CANTACUZÈNE) AND HIS FRENCH CONNECTIONS.
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Cavaillon, Jean-Marc and Ancuta, Petronela
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FRENCH language , *SCARLATINA , *IONS - Published
- 2022
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49. New insights into the heterogeneity of Th17 subsets contributing to HIV-1 persistence during antiretroviral therapy.
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Wacleche, Vanessa Sue, Goulet, Jean-Philippe, Gosselin, Annie, Monteiro, Patricia, Soudeyns, Hugo, Fromentin, Rémi, Jenabian, Mohammad-Ali, Vartanian, Shant, Deeks, Steven G., Chomont, Nicolas, Routy, Jean-Pierre, and Ancuta, Petronela
- Subjects
HIV ,HETEROGENEITY ,T helper cells ,HIGHLY active antiretroviral therapy ,LYMPH nodes - Abstract
Background: Th17 cells are permissive to HIV-1 infection and their depletion from the gut of infected individuals leads to microbial translocation, a major cause for non-AIDS co-morbidities. Most recent evidence supports the contribution of long-lived Th17 cells to HIV persistence during antiretroviral therapy (ART). However, the identity of long-lived Th17 cells remains unknown. Results: Here, we performed an in-depth transcriptional and functional characterization of four distinct Th17 subsets and investigated their contribution to HIV reservoir persistence during ART. In addition to the previously characterized CCR6
+ CCR4+ (Th17) and CCR6+ CXCR3+ (Th1Th17) subsets, we reveal the existence of two novel CCR6+ subsets, lacking (double negative, CCR6+ DN) or co-expressing CXCR3 and CCR4 (double positive, CCR6+ DP). The four subsets shared multiple Th17-polarization markers, a fraction of cells proliferated in response to C. albicans, and exhibited lineage commitment and plasticity when cultured under Th17 and Th1 conditions, respectively. Of note, fractions of CCR6+ DN and Th17 demonstrated stable Th17-lineage commitment under Th1-polarization conditions. Among the four subsets, CCR6+ DN expressed a unique transcriptional signature indicative of early Th17 development (IL-17F, STAT3), lymph-node homing (CCR7, CD62L), follicular help (CXCR5, BCL6, ASCL2), and self-renewal (LEFI, MYC, TERC). Cross sectional and longitudinal studies demonstrated that CCR6+ DN cells were the most predominant CCR6+ subset in the blood before and after ART initiation; high frequencies of these cells were similarly observed in inguinal lymph nodes of individuals receiving long-term ART. Importantly, replication competent HIV was isolated from CCR6+ DN of ART-treated individuals. Conclusions: Together, these results provide new insights into the functional heterogeneity of Th17-polarized CCR6+ CD4+ T-cells and support the major contribution of CCR6 DN cells to HIV persistence during ART. [ABSTRACT FROM AUTHOR]+ - Published
- 2016
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50. Identification of novel HIV-1 dependency factors in primary CCR4+CCR6+Th17 cells via a genome-wide transcriptional approach.
- Author
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Cleret-Buhot, Aurélie, Yuwei Zhang, Planas, Delphine, Goulet, Jean-Philippe, Monteiro, Patricia, Gosselin, Annie, Wacleche, Vanessa Sue, Tremblay, Cécile L., Jenabian, Mohammad-Ali, Routy, Jean-Pierre, El-Far, Mohamed, Chomont, Nicolas, Haddad, Elias K., Sekaly, Rafick-Pierre, and Ancuta, Petronela
- Subjects
HIV infection risk factors ,T cells ,REGENERATION (Biology) ,MOLECULAR structure of transcription factors ,GENOMICS - Abstract
Background: The HIV-1 infection is characterized by profound CD4
+ T cell destruction and a marked Th17 dysfunction at the mucosal level. Viral suppressive antiretroviral therapy restores Th1 but not Th17 cells. Although several key HIV dependency factors (HDF) were identified in the past years via genome-wide siRNA screens in cell lines, molecular determinants of HIV permissiveness in primary Th17 cells remain to be elucidated. Results: In an effort to orient Th17-targeted reconstitution strategies, we investigated molecular mechanisms of HIV permissiveness in Th17 cells. Genome-wide transcriptional profiling in memory CD4+ T-cell subsets enriched in cells exhibiting Th17 (CCR4+ CCR6+ ), Th1 (CXCR3+ CCR6- ), Th2 (CCR4+ CCR6- ), and Th1Th17 (CXCR3+ CCR6+ ) features revealed remarkable transcriptional differences between Th17 and Th1 subsets. The HIV-DNA integration was superior in Th17 versus Th1 upon exposure to both wild-type and VSV-G-pseudotyped HIV; this indicates that post-entry mechanisms contribute to viral replication in Th17. Transcripts significantly enriched in Th17 versus Th1 were previously associated with the regulation of TCR signaling (ZAP-70, Lck, and CD96) and Th17 polarization (RORγt, ARNTL, PTPN13, and RUNX1). A meta-analysis using the NCBI HIV Interaction Database revealed a set of Th17-specific HIV dependency factors (HDFs): PARG, PAK2, KLF2, ITGB7, PTEN, ATG16L1, Alix/AIP1/PDCD6IP, LGALS3, JAK1, TRIM8, MALT1, FOXO3, ARNTL/BMAL1, ABCB1/MDR1, TNFSF13B/BAFF, and CDKN1B. Functional studies demonstrated an increased ability of Th17 versus Th1 cells to respond to TCR triggering in terms of NF-κB nuclear translocation/DNA-binding activity and proliferation. Finally, RNA interference studies identified MAP3K4 and PTPN13 as two novel Th17-specific HDFs. Conclusions: The transcriptional program of Th17 cells includes molecules regulating HIV replication at multiple post-entry steps that may represent potential targets for novel therapies aimed at protecting Th17 cells from infection and subsequent depletion in HIV-infected subjects. [ABSTRACT FROM AUTHOR]- Published
- 2015
- Full Text
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