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[Objective] To establish a rapid method for determining the volatile components of Dracocephalum moldavica by headspace solid phase microextraction-gas chromatography-mass spectrometry. [Method] This method was used to determine and analyze the volatile components of Dracocephalum moldavica. Qualitative analysis was conducted based on chromatographic peaks and retention time,and quantitative analysis was performed using area normalization method to calculate the relative percentage content of each component. [Result]A total of 59 volatile components were detected, including aldehydes ( 59. 50%), esters ( 13. 61%), olefins ( 10. 99%), ketones ( 8. 13%), alcohols (4. 82%),heterocycles (2. 82%) and furans (0. 13%). [Conclusion]The use of this method has the advantages of low sample size,omitting the sample pre-treatment process,preventing impurities brought in by organic reagents from interfering with the analysis results,reducing pollution to the chromatographic column and sample inlet,and reducing the loss of volatile components during the analysis process. It can effectively separate volatile components in the sample and is suitable for the detection of volatile components in Dracocephalum moldavica. [ABSTRACT FROM AUTHOR]

We introduced a Tomato Orchard Visual Feature Management System based on the . NET platform, aimed at improving the efficiency of tomato detection and recognition and promoting the intelligent development of agricultural production. With the development and popularization of information technology, more and more agricultural production activities are beginning to shift towards digitization and intelligence. At the same time, with the acceleration of population growth and urbanization, people's demand for vegetables and fruits is increasing, and the requirements for fruit quality are also increasing. As one of the important economic crops in modern agriculture, tomato cultivation and management are crucial for fruit farmers. In order to improve the production efficiency and quality of tomato cultivation, the system was used to help fruit farmers manage the entire process of tomato cultivation. Taking a tomato orchard located in the Lushunkou District of Dalian of Liaoning Province as an example, the system framework, including the system's data results and framework, the processing and data analysis of sensor data and image data, was constructed to achieve real-time monitoring and management of the growth status and quality of tomatoes in the orchard, improving the production efficiency and quality of the tomato orchard, and increasing the production and economic benefits of fruit farmers. [ABSTRACT FROM AUTHOR]

Catalytic oxidation technology is highly efficient in the end-of-pipe treatment of VOCs. In recent years, cobalt-based compounds derived from metal-organic frameworks (MOFs) have shown excellent performance in catalytic oxidation of VOCs. The composition and preparation methods of four typical cobalt-based MOFs (ZIF-67, ZSA-1, Co-MOF-71 and Co-MOF-74) and their derived co- balt-based catalysts were summarized. The catalytic oxidation of several typical VOCs (toluene, o-xy- lene, formaldehyde and acetone) by cobalt-based catalysts was reviewed. The effects of space velocity and humidity on the catalytic oxidation of VOCs by cobalt-based catalysts were outlined. Finally, the reaction mechanism of cobalt based catalysts derived from MOFs was discussed, and an outlook on their research in the oxidation of VOCs was given. [ABSTRACT FROM AUTHOR]

We quantitatively evaluated and simulatied of outdoor public space thermal comfort in traditional villages, so as to optimize rural public space and living environment. Taking Lefeng village, Chenggu county, Hanzhong city, Shaanxi province as an example, We selected the parameters of temperature, humidity, wind speed and thermal comfort at three different locations in the village to conduct microclimate simulation by using field space survey and ENVI-Met technology. The results show that Lefeng village, featuring a certain scale of historical buildings, has a good eco-climate adaptability on the whole. However, the local microclimate is not suitable, and the microclimate of different areas in the village is different. The wind environment of the village streets is superior, the ventilation condition is good, and the outdoor thermal comfort is good. However, in traditional building blocks, the temperature and humidity changes significantly due to the reasons of building enclosure. Therefore, to improve the living environment of Lefeng village and optimize the village space, we can combine the public space of villagers' activities with the roadway, and increase the interaction of villagers' activities by using a better wind environment. In the courtyard, as the building is a listed historical building, it cannot be reconstructed, so the outdoor comfort can only be improved by adding garden greening, water and other means. Finally, it provides a reference for improving the design method of village spatial form and green village planning and design practice. [ABSTRACT FROM AUTHOR]

Photodynamic therapy (PDT) is a non-invasive treatment method that combines light sources and photosensitizers. It is currently widely applied in clinical practice to treat acne, genital warts, and multiple tumor diseases. The mechanism of PDT is complex. Researches have found that the efficacy of PDT seems to be closely related to the degree of acute inflammation it causes. IL-6 is a common pro-inflammatory factor that belongs to the category of interleukin (IL). It is produced by fibroblasts, monocytes/macrophages, and T lymphocytes. It exerts multiple effects on inflammation and immune responses. IL-6 can be induced by PDT in vivo and in vitro. In this article, research progress in the application of IL-6 in PDT was reviewed. [ABSTRACT FROM AUTHOR]

近年来,全国各高校开启了大规模在线教学实践,中国高校的在线教育得到前所未有发展,但 在线“课堂沉默”现象日益突出,成为阻碍师生教与学的痛点问题。该研究通过对高职院校学生问卷调查和个 别访谈,研究学生在线“课堂沉默”现象的情况及其背后因素。该研究表明,高职学生在线“课程沉默”现象严 重,这与网络信息化建设、教师授课方法得当与否及学生自身因素均有关系。只有学校、教师、学生三方面共同 努力,才能减轻高职学生在线“课程沉默”,从而保证在线教学效果。 [ABSTRACT FROM AUTHOR]

The Constitution of Lumen Gentium in the Second Vatican Council regards the papal authority as a collegial power, emphasizing that the Pope and bishops belong to the same college; that they present the collective power of the church as a whole. This kind of understanding symbolizes a massive reform of the church hierarchy, but it does not take the authority away from the Pope; on the contrary, it reveals the dynamic process of intention of the collective authority. This formation of collective authority in the Lumen Gentium will be very enlightening for the further self-reformation of the Catholic Church and the ecumenical movement as well. [ABSTRACT FROM AUTHOR]

Gestational metabolic diseases (GMD) are the most common cause of maternal and neonatal outcomes, but the mechanism remains unclear. Fibroblast growth factor 21 (FGF21) is a polypeptide hormone synthesized by a variety of organs. Its mechanism of action is complex. It regulates a series of metabolic processes in vitro and in vivo, and mainly participates in metabolic activities such as glucose, lipid, energy regulation and hepato -intestinal circulation of bile acid by means of endocrine. It also plays a key role in cell proliferation and differentiation, neuronal development, angiogenesis and wound healing. Therefore, FGF21 release disorders, signal dysregulation, and abnormal function are closely associated with GMD such as gestational diabetes mellitus, gestational hypertension disease, and intrahepatic cholestasis of pregnancy. This article reviews the latest research progress on the mechanism of FGF21 in GMD, so as to identify GMD and its hidden disease as early and accurate as possible, achieve the purpose of early diagnosis and intervention, and ultimately improve the prognosis of mothers and infants. [ABSTRACT FROM AUTHOR]

BACKGROUND: Neuroinflammation affects the progression of amyotrophic lateral sclerosis. In vitro studies have shown that neural stem cells have antiinflammatory effect, but whether they can be anti-inflammatory in amyotrophic lateral sclerosis mouse, and the mechanism and optimal transplantation route are still unclear. OBJECTIVE: To investigate the anti-inflammatory effect and mechanism of neural stem cell transplantation in different ways on amyotrophic lateral sclerosis model G93A-SOD1 mice. METHODS: Neural stem cells were isolated and identified, transfected with green fluorescent protein. Totally 78 G93A-SOD1 mice aged 70 days were divided into lateral ventricle group, tail vein group, and control group. After adaptive feeding, 5×105 green fluorescent protein-transfected neural stem cells were transplanted into the lateral ventricle, and 1×106 green fluorescent protein-transfected neural stem cells were injected through the tail vein at the age of 84 days. From the age of 85 days, modified Wrathall motor score and rotation test were performed every week to evaluate motor function. Onset time, disease duration and survival time were recorded. At the age of 105 days, immunofluorescence method was conducted to detect survival, migration and differentiation of neural stem cells transfected with green fluorescent protein. The number of motor neurons was measured by Nissl staining. The expression of ChAT was detected by western blot assay. Immunofluorescence was used to detect the expression of NeuN in spinal cord anterior horn motor neurons. The protein and mRNA levels of tumor necrosis factor alpha, interleukin 1 beta, interleukin 6 and transforming growth factor beta in cerebrospinal fluid were detected by ELISA and RT-PCT. Western blot assay was used to detect the expression of iNOS, CD206 and NF-κB pathway proteins in the spinal cord. The pathological changes of gastrocnemius muscle were detected by hematoxylin-eosin staining. RESULTS AND CONCLUSION: (1) The extracted neural stem cells grew and differentiated well and could be transfected by green fluorescent protein. (2) The lateral ventricle pathway was more favorable for neural stem cells to enter the central nervous system in the super-early stage after the onset of disease, and decreased the levels of tumor necrosis factor alpha, interleukin 1 beta, interleukin 6, increased the level of tumor necrosis factor-β, reduced the expression of inducible nitric oxide synthase and increased the expression level of CD206, inhibited the activation of NF-κB pathway, slowed down the progression of motor function damage, and reduced the degree of pathological damage of skeletal muscle. (3) In the super-early stage after the onset of the disease, the effects of two ways of transplanting neural stem cells to protect the anterior horn motor neurons and prolong the course of disease and survival time of mice were limited. (4) Results show that in the super early stage after the onset of amyotrophic lateral sclerosis, the lateral ventricle may be a better way of transplanting neural stem cells, which has a good anti-inflammatory effect. The mechanism may be regulating the polarization direction of microglia and inhibiting the activation of NF-κB pathway. BACKGROUND: Neuroinflammation affects the progression of amyotrophic lateral sclerosis. In vitro studies have shown that neural stem cells have antiinflammatory effect, but whether they can be anti-inflammatory in amyotrophic lateral sclerosis mouse, and the mechanism and optimal transplantation route are still unclear. OBJECTIVE: To investigate the anti-inflammatory effect and mechanism of neural stem cell transplantation in different ways on amyotrophic lateral sclerosis model G93A-SOD1 mice. METHODS: Neural stem cells were isolated and identified, transfected with green fluorescent protein. Totally 78 G93A-SOD1 mice aged 70 days were divided into lateral ventricle group, tail vein group, and control group. After adaptive feeding, 5×105 green fluorescent protein-transfected neural stem cells were transplanted into the lateral ventricle, and 1×106 green fluorescent protein-transfected neural stem cells were injected through the tail vein at the age of 84 days. From the age of 85 days, modified Wrathall motor score and rotation test were performed every week to evaluate motor function. Onset time, disease duration and survival time were recorded. At the age of 105 days, immunofluorescence method was conducted to detect survival, migration and differentiation of neural stem cells transfected with green fluorescent protein. The number of motor neurons was measured by Nissl staining. The expression of ChAT was detected by western blot assay. Immunofluorescence was used to detect the expression of NeuN in spinal cord anterior horn motor neurons. The protein and mRNA levels of tumor necrosis factor alpha, interleukin 1 beta, interleukin 6 and transforming growth factor beta in cerebrospinal fluid were detected by ELISA and RT-PCT. Western blot assay was used to detect the expression of iNOS, CD206 and NF-κB pathway proteins in the spinal cord. The pathological changes of gastrocnemius muscle were detected by hematoxylin-eosin staining. RESULTS AND CONCLUSION: (1) The extracted neural stem cells grew and differentiated well and could be transfected by green fluorescent protein. (2) The lateral ventricle pathway was more favorable for neural stem cells to enter the central nervous system in the super-early stage after the onset of disease, and decreased the levels of tumor necrosis factor alpha, interleukin 1 beta, interleukin 6, increased the level of tumor necrosis factor-β, reduced the expression of inducible nitric oxide synthase and increased the expression level of CD206, inhibited the activation of NF-κB pathway, slowed down the progression of motor function damage, and reduced the degree of pathological damage of skeletal muscle. (3) In the super-early stage after the onset of the disease, the effects of two ways of transplanting neural stem cells to protect the anterior horn motor neurons and prolong the course of disease and survival time of mice were limited. (4) Results show that in the super early stage after the onset of amyotrophic lateral sclerosis, the lateral ventricle may be a better way of transplanting neural stem cells, which has a good anti-inflammatory effect. The mechanism may be regulating the polarization direction of microglia and inhibiting the activation of NF-κB pathway. Key words: neural stem cell; amyotrophic lateral sclerosis; inflammation; transplantation pathway; microglia; NF-κB pathway; motor neuron; inflammatory cytokine [ABSTRACT FROM AUTHOR]

During the work of slurry pump, the hard-solid particles in the slurry will cause strong erosion and wear on the pump wall, resulting in lower efficiency and shorter service life of the slurry pump. In order to solve the problems of low efficiency and fast wear rate of slurry pump, the impeller blade profile of slurry pump was modified by using the variable angle spiral method (VASM), and the influence of different particle size, concentration and density on the performance of slurry pump with different profiles was studied. Firstly, the variable angle helix method and discrete phase model were introduced, and the numerical calculation model was verified. Then, on the premise that the front and rear cover plates of impeller and the profile of blade were unchanged, the VASM was used to modify the design of the LC100/ 350 slurry pump blades. The pump inner two-phase flows were simulated by using Reynolds-average Navier-Stokes equation, and the RNG k- & turbulence model, discrete phase model (DPM) and SIMPLE algorithm. To assess the improvement effect, the lead, efficiency and wear strength of the pump under rated working condition were taken as the improved performance index of the slurry pump. Finally, the relationship between the blade profile and the hydraulic performance and abrasion performance of the pump was analyzed using CFD numerical simulation. The calculation results show that the blade designed by VASM, can reduce the erosion strength, improve the efficiency obviously and have a little effect on the head. Although the increase of particle size and concentration reduce the efficiency and increase the wear strength, the change of particle size and concentration cannot affect the selection of the optimal blade shape. Considering the hydraulic performance and erosion strength of the slurry pump, the blade wrap angle of 140° is optimal. [ABSTRACT FROM AUTHOR]

objective To investigate the immunological characteristics of Acanthamoeba castellanii (Ac) Actin (Actin 1) (Ac-Action 1), and to clarify the role of Ac-Actin 1 in adhesion to the host cells and invasion of Ac parasite preliminarily. Methods The Ac trophozoite cDNA was regarded as the template, and the prokaryotic expression vector pET 22b (+)-Ac-Actin 1 was constructed and transformed into the Escherichia coli competent cells BL21 (DE3)；the recombinant Ac-Actin 1 protein (rAc-Actin 1) was induced by 1 mmol·L-1 isopropyl β-D-thiogalactoside (IPTG) in vitro, and the solubility of rAc-Actin 1 was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)；the rAc-Actin 1 with His label was purified by affinity chromatography. Three New Zealand white rabbits were randomly divided into control group (n=1) and experiment group (n=2)； the rabbits in experiment group were subcutaneously injectied with 400 μg rAc-Actin 1 as the immunogen and the rabbit in control group was injected with the same amount of normal saline, and the anti-rAc-Actin 1 polyclonal serum of the rabbits was prepared；the titers of anti-rAc-Actin 1 polyclonal antibody of the rabbits in two groups were determined by enzyme linked immunosorbent assay (ELISA) method and the IgG subtype was determined； Western blotting method was used to detect the immunoreactivity of anti-rAc-Actin 1 polyclonal rabbit serum and rAc-Actin 1；the localization of Ac-Actin 1 in the Actrophozoite was detected by the indirect immunofluorescence assay (IFA). The normal trophozoites were used as controls, the anti-rAc-Actin 1 polyclonal serum of the rabbits blocked the Ac trophozoites and was co-incubated with the Vero cells, and the adhesion effect of Ac-Actin 1 on the Vero cells was observed by microscope. Results The SDS-PAGE and BAC concentration detection results showed that the high concentration of rAc-Actin 1 (1.7 g·L-1) protein was obtained.The ELISA results showed that the titer of the obtained rabbit anti-rAc-Actin 1 specific polyclonal antibody was 1∶6 400, and the concentrations of IgG1 and IgG2a were 116.76 g·L-1 and 1 136.15 mg·L-1, respectively.The Western blotting results showed that the rabbit anti-rAc-Actin 1 polyclonal antibody could specifically bind to the rAc-Actin 1 and had good immunoreactivity.The IFA results showed that rAc-Actin 1 mainly located on the cell membrane of Ac trophozoites.The microscope analysis results showed that compared with control group, the adhesion rate of Ac trophozoites to the Vero cells in the experiment group was significantly decreased (P<0.01) with the prolongation of incubation time of antibody and parasite, and the anti-rAc-Actin 1 specific polyclonal antibody could effectively block the adhesion of Ac trophozoites to the Vero cells. Conclusion rAc-Actin 1 has good immunogenicity and immunoreactivities, which is involved in the adhesion between the Ac parasite and the host cells. [ABSTRACT FROM AUTHOR]

Objective To study the clinical characteristics and perinatal outcomes of patients with premature preeclampsia (PE) and chronic hypertension complicated with PE, and to compare the difference of triglyceride-glucose index (TyG) between the two groups of patients. Methods A total of 158 cases of pregnancy terminated by cesarean section at 28-36+6 weeks of gestation due to "preeclampsia" were selected. According to whether they had chronic hypertension, patients were divided into the chronic hypertension PE group (53 cases) and the PE group (105 cases). The general data, blood routine, coagulation routine, biochemical indexes and TyG were compared between the two groups, and the difference in perinatal outcomes was analyzed. Results Compared with the PE group, patients in the chronic hypertension PE group had older age, higher gestational weight gain during pregnancy, and the gestational age of delivery is earlier, and the difference between the two groups was statistically significant (P＜0.05). Data of hematocrit, platelet, fibrinogen, creatine kinase isoenzyme, aspartate aminotransferase, total cholesterol, triglyceride, fasting blood glucose and 24-hour urinary protein were significantly increased in the chronic hypertension PE group, while uric acid was lower (P＜0.05). TyG index was also higher in the chronic hypertension PE group (9.89±0.30 vs. 9.76±0.26, P＜0.01). The neonatal birth weight and 1- minute Apgar score were lower in the chronic hypertension PE group, but the arterial blood alkaloid surplus was higher and the length of hospital stay in NICU was longer (P＜0.05). For both types of preterm PE, the preterm delivery near term (34- 36+6weeks of gestation) accounted for the largest proportion, but the early preterm birth (28-31+6weeks of gestation) was higher in the chronic hypertension PE group (28.3% vs. 10.5%, P＜0.01), and the proportion of small-gestational age babies (SGA) was also higher (60.4% vs. 42.9%, P＜0.05). Conclusion Compared with preterm PE, patients with preterm chronic hypertension PE have more significant insulin resistance, hypercoagulability, myocardial and kidney damage, and higher risk of early preterm delivery and SGA. Maternal and fetal monitoring should be more intensive. [ABSTRACT FROM AUTHOR]

以上海的医院为例, 通过梳理医院科技成果转化作价入股的政策和现状, 剖析当前实施中存在的主要困难, 并提出相应的应对策略, 以期打通医院作价入股实施科技成果转化的链路, 助力实现创新驱动发展战略的实行. [ABSTRACT FROM AUTHOR]

Using the liquid-solid waste in the synthesis process of bisphenol A epoxy resin as raw material, the activated carbon with high adsorption performance was prepared by chemical activation method・ The effects of activator type, raw material liquid-solid ratio, activation temperature, activation time and impregnation ratio on the yield and adsorption performance of activated carbon were investigated・ The results showed that with KOH as the best activator, the activated carbon prepared under the cond让ions of raw terial liquid-solid mass ratio of 3 ： 7, impregnation ratio of 1, activation temperature of 800 尤, and activation time of 60 min was 35. 28% in yield, and the adsorption capacity of methylene blue was 1 531 mg/g・ The morphology, structure and surface functional groups of activated carbon were characterized by BET, SEM, FTIR, XRD, Bohem titration and other means・ It can be seen that the activated carbon is amorphous and has a well・developed pore structure on the surface・ The specific surface area is 1 693 m2/g, the total pore volume is 0・ 754 0 cm3/g, the average pore diameter is 1. 782 nm, and the micropore volume accounts for 82. 73% of the total pores・ The results of FTIR and Bohem titration showed that the surface of activated carbon contained oxygen-containing functional groups such as phenolic hydroxyl group and alcoholic hydroxyl group, and its total acidity was 3・ 34 mmo. [ABSTRACT FROM AUTHOR]

BACKGROUND: Wentong Huoxue Cream can improve oxidative stress, reduce blood viscosity, restore microcirculation, relieve diabetic peripheral nerve injury, and inhibit sensory conduction and pain formation, but its regulatory mechanism remains unclear. OBJECTIVE: To observe the effects of Wentong Huoxue Cream on JNK signaling pathway proteins and inflammatory factors in diabetic peripheral neuropathy rats. METHODS: A rat model of diabetic peripheral neuropathy was established in adult Wistar rats. Forty diabetic peripheral neuropathy rats were randomly divided into five groups (n=8 per group): model group, low-, middle- and high-dose Wentong Huoxue Cream groups (given 2 mL of 0.75, 1.5, 3.0 g/mL Wentong Huoxue Cream to the lower limbs of rats, respectively) and Western medicine group (given 2 mL of control cream). Skin treatment in each group was performed twice a day for 28 days. Additionally, six rats were taken as normal group. After the last administration, nerve conduction tests were performed and the expressions of interleukin-6, interleukin-18, tumor necrosis factor α, high-sensitivity C-reactive protein, and myelin basic protein in serum were detected by ELISA. The expression of JNK and P-JNK protein in sciatic ganglia was detected by western blot and the expression of JNK mRNA was determined by RT-PCR. RESULTS AND CONCLUSION: The velocity of sensory nerve conduction and motor nerve conduction was significantly decreased in the model group compared with the normal group (P < 0.05). Compared with the model group, the sensory nerve conduction velocity increased significantly after intervention with different doses of Wentong Huoxue Cream (P < 0.05), especially in the high-dose Wentong Huoxue Cream group (P < 0.05). Compared with the model group, the motor nerve conduction velocity was significantly increased in the high-dose Wentong Huoxue Cream group and Western medicine group (P < 0.05). Compared with the normal group, the serum levels of interleukin-6, tumor necrosis factor α, high-sensitivity C-reactive protein, and myelin basic protein were significantly increased (P < 0.05) and the serum level of interleukin-18 was significantly decreased in the model group (P < 0.05). Compared with the model group, the serum levels of interleukin-6, interleukin-18, tumor necrosis factor α, high-sensitivity C-reactive protein, and myelin basic protein were significantly decreased in all the treatment groups (P < 0.05), especially in the high-dose Wentong Huoxue Cream group and Western medicine group. Compared with the normal group, p-JNK protein and JNK mRNA expression levels were significantly increased in the model group (P < 0.05). Compared with the model group, p-JNK protein and JNK mRNA expression levels were significantly decreased in the middle- and high-dose Wentong Huoxue Cream groups and Western medicine group (P < 0.05). Therefore, Wentong Huoxue Cream can inhibit the abnormal activation of JNK signal transduction pathway, reduce inflammatory reactions, and protect the peripheral nerve function of diabetic peripheral neuropathy rats. [ABSTRACT FROM AUTHOR]

To deal with the large amount of physical noise, redundant features unrelated to temperature, and time correlation between massive meteorological observational data, a multi-information fusion temperature forecast method combining one-dimensional convolutional neural network (1DCNN) and long short-term memory neural network (LSTM) is proposed. First, the difference method is used to preprocess the meteorological observational data to obtain stationary time series data. Second, 1DCNN is used to extract feature variables related to temperature changes as the input variables of the neural network model. Finally, 1DCNN and LSTM are used to establish a multi-information fusion temperature prediction model 1DCNN-LSTM. Taking the historical meteorological observational data of Kunming City in Yunnan Province as an example, the model is compared with the traditional LSTM, 1DCNN and Back Propagation Neural Network (BP) of the hourly temperature forecast in the next 24 hours. The results show that compared with those of LSTM, 1DCNN and BP, the root mean square error (RMSE) of 1DCNN-LSTM is reduced by 5.221%, 19.350%, and 9.253%, and the mean absolute error (MAE) is reduced by 4.419%, 17.520% and 8.089%, respectively. This research method provides a reference for the accurate prediction of air temperature. [ABSTRACT FROM AUTHOR]

Virtual synchronous generator (VSG) control technology is used in power electronic converter, which simulates the transient characteristics of synchronous generator, endows the power grid with inertia and damping functions, and has good frequency support capability and power grid compatibility. This paper presents a comprehensive analysis method of VSG control system performance, which can be used as the theoretical basis for designing VSG controller. Firstly, the equivalent model of VSG is established on the basis of analyzing the working principle of VSG. Secondly, the small signal model of VSG is established, and the related transfer functions of active power reference value, reactive power reference value and grid frequency as input, active power output and reactive power output as output are derived. Then, based on the transfer function, the relationship between the system steady-state performance, transient performance and VSG parameters is obtained, and the influence of the parameters on the system performance is analyzed. Finally, the correctness of relevant theoretical analysis is verified by simulation. [ABSTRACT FROM AUTHOR]

In this paper, a recognition method is proposed based on the time-frequency domain and PE features of ship radiated noise. The empirical mode decomposition (EMD) method is used to decompose the original signal into multiple Intrinsic modal function (IMF) and screen them combined with the reverse weighted permutation entropy (RWPE). By taking the weighted average instantaneous frequency and weighted permutation entropy (WPE) of effective IMF as inputs the LSTM neural network is used for training. The experimental results show that the recognition rate can reach 99.40%, compared with the ship-single-feature-based recognition method. [ABSTRACT FROM AUTHOR]

BACKGROUND: In clinic, guided bone regeneration technology is often used to reconstruct the bone defect. That is, the scaffold material is implanted in the bone defect, and then the biological barrier membrane is used to establish a stable environment to ensure the proliferation of osteoblasts and the formation of blood vessels, which has achieved good results. OBJECTIVE: To observe the osteogenic effect of true bone ceramic combined with acellular dermal matrix in tooth socket filling. METHODS: Bovine cancellous bone was calcined to true bone ceramic. The acellular dermal matrix was prepared from split-thickness skin which the epidermis and cellular components have been extracted. Cytotoxicities of true bone ceramic and acellular dermal matrix were evaluated by MTT assay. The second and fourth premolars on both sides of the mandible were extracted in nine beagle dogs. The true bone ceramic was implanted into the socket and covered with acellular dermal matrix. The buccal and lingual gingivae were separated and sutured to close the extraction wound. At 1, 3, and 6 months after surgery, cone beam CT, X-ray and histology were used to evaluate the new bone formation. The experiment was approved by Drug Safety Evaluation Center in China Institute for Radiation Protection (approval No. CIRP-IACUC-（R）2018081). RESULTS AND CONCLUSION: (1) L929 mouse fibroblasts were cultured with the two extracts for 24 hours. Under the inverted microscope, the cells were in good shape without vacuoles or death. MTT assay results showed that the cell survival rate was above 85%, without obvious cytotoxicity. (2) The results of cone beam CT showed that the relative gray value of the implant site increased gradually with the extension of healing time, and the amount of bone formation in tooth extraction wound increased.(3) The X-ray films showed that at 1 month after the operation, the imaging of the implanted site was uniform without obvious osteogenesis. At 3 months after the operation, there was partial osteogenesis. At 6 months after the operation, the implant site had obvious osteogenesis. (4) Hematoxylin-eosin staining showed that a large number of trabeculae were formed 1 month after operation, and the bone trabeculae was evenly arranged, and the true bone ceramic was partly absorbed. Coarse trabeculae structure with a large amount of cartilage and a small amount of calcined bone remained at 3 months after operation. The tooth extraction wound was nearly healed and mature bone tissue was formed, and the bone unit structure was visible; true bone ceramic could not be seen at 6 months after operation. (5) Results suggest that true bone ceramic combined with acellular dermal matrix show good bone formation for extraction socket filling. [ABSTRACT FROM AUTHOR]

Peutz -Jeghers syndrome is an autosomal dominant genetic disease, often accompanied by a variety of gynecological tumors, and the tumor type is relatively rare, which is prone to misdiagnosis and missed diagnosis in clinical practice. We report a case of Peutz-Jeghers syndrome with ovarian sex cord tumor with annular tubules, ovarian mucinous tumor and cervical minimal deviation adenocarcinoma admitted to the First Affiliated Hospital of Dalian Medical University. The patient presented to the hospital for the first time with pelvic mass and was diagnosed as ovarian mucinous cystadenoma after ovarian tumor stripping. It was complicated with microscopic ovarian sex cord tumor with annular tubules, but the tumor recurred within a short time after the removal of the tumor twice, and eventually progressed to ovarian mucinous cystadenocarcinoma. The third operation was accidentally found to be accompanied by cervical minimal deviation adenocarcinoma. The clinical features, diagnosis and treatment process and postoperative follow-up of the patient are reviewed to improve clinicians′ cognition of gynecologic tumors associated with Peutz-Jeghers syndrome. [ABSTRACT FROM AUTHOR]

The large-scale breeding level of livestock and poultry industry in Hubei Province has been significantly improved, and the amount of straw resources has been increased year by year. Returning agricultural wastes to the field can not only reduce agricultural non-point source pollution, but also reduce the application of chemical fertilizers. Based on relevant statistical data and literature, this study collected the amount of livestock and poultry (i.e., pigs, beef cattle, dairy cows, sheep, broilers, layers) in stock, market and growth cycle, and the planting area and economic yield of various crops (i.e., the low hilly area in southeastern Hubei, the low hilly area in northern Hubei, the mountainous area in northwestern Hubei, the plain area in central Hubei, and the mountainous area in southwestern Hubei). Based on the statistical data from 2019 to 2020, We calculated the nutrient supply of livestock and poultry waste, and further evaluated whether the current situation of livestock and poultry breeding in Hubei Province exceeded the maximum allowable amount of soil carrying livestock and poultry waste. According to the ratio of straw to grain of different crops and nutrient content of straw, we analyzed the nutrient resources and the theoretical nutrient returning amount of crop straw. The land carrying capacity index of livestock was the highest in Northern Hubei, reaching to 0.35-0.78. The land carrying capacity index of Central Hubei was only 0.17-0.54, indicating that there would be a large space to develop livestock and poultry breeding. In 2019, the nutrient resources of livestock and poultry waste in Hubei Province were 368 900 t N, 140 300 t P2O5 and 520 600 t K2O, respectively. Based on 65% of livestock manure and fertilizer returning to the field, the total nutrient returning to the field of livestock and poultry manure would be 239 800 t N, 91 200 t P2O5 and 337 700 t K2O, respectively, with the ratio of fertilizer replacement of 17.3%, 11.9% and 56.2%, respectively. The total amount of straw resources of main crops in Hubei Province was the highest in Central Hubei and the lowest in Northwest Hubei. At present, the nutrient resources of straw in Hubei province were 310 700 t N, 99 800 t P2O5 and 683 000 t K2O, and the proportion of theoretical fertilizer replacement were 22.53%, 13.11% and 113.97%, respectively. The livestock and poultry production in different regions of Hubei province did not exceed the local maximum carrying capacity. The livestock and poultry breeding volume in different regions of Hubei Province did not exceed the local maximum carrying capacity. The theory of returning major agricultural wastes in Hubei Province can reduce nitrogen fertilizer consumption by 39.8% and phosphate fertilizer consumption by 25.0%. The total K amount from agricultural wastes return to the field could theoretically meet the demand for potassium of main crops. By calculating the nutrient resources of the main agricultural wastes (i.e., livestock and poultry waste, and straw) in Hubei Province, we evaluated the potential of fertilizer reduction of agricultural waste returning to the field and the carrying capacity of livestock and poultry waste soil, providing theoretical basis and data support for agricultural green development in Hubei Province. [ABSTRACT FROM AUTHOR]

目的　探究血清促黄体生成素（LH）、抗缪勒管激素（AMH）、促卵泡生成素（FSH）与剖宫产瘢痕妊娠 （CSP）子宫动脉栓塞术后再妊娠的关系。方法　选取86例CSP患者，均采用子宫动脉栓塞术治疗。统计术后3年 再妊娠情况，收集术后3年内患者临床资料（年龄、孕次、剖宫产次数、月经恢复时间及瘢痕病灶直径）；术前及术后 第1、3、6个月经周期第2～4 d用电化学发光法检测血清LH、AMH、FSH；用Logistic回归分析CSP患者子宫动脉栓塞 术后再妊娠的影响因素；绘制受试者工作特征曲线，用曲线下面积分析血清LH、AMH、FSH水平对CSP患者子宫动 脉栓塞术后再妊娠的预测价值。结果　术后3年再妊娠53例（再妊娠组），再妊娠率61. 6%（53/86）；未妊娠33例 （未妊娠组）。两组年龄、孕次、剖宫产次数、月经恢复时间及病灶直径比较差异无统计学意义（P 均>0. 05）。术后第 1、3、6个月经周期妊娠组LH、FSH 水平低于未妊娠组，AMH 水平高于未妊娠组，比较差异有统计学意义（P 均< 0. 05）。Logistic回归分析显示，术后血清LH、AMH、FSH水平均与CSP患者子宫动脉栓塞术后再妊娠独立相关（P 均<0. 05）。术后第6个月经周期血清LH、AMH、FSH预测CSP患者子宫动脉栓塞术后再妊娠的曲线下面积分别为 0. 736、0. 757、0. 826，三者联合预测的曲线下面积为0. 925，血清LH、AMH、FSH联合预测的曲线下面积高于三者单 独预测（P 均<0. 05）。结论　子宫动脉栓塞术后第6个月经周期血清LH、AMH、FSH联合检测对CSP患者再妊娠的 预测价值较高。 [ABSTRACT FROM AUTHOR]

With the change of lifestyle and the aggravation of population aging, the incidence of osteoporosis is increasing year by year. Osteoporosis has gradually become a major public health problem. Bone is a relatively dynamic organ that provides stiffness, shape, support, and movement to the body structure. Bone strength is directly related to mortality and injury risk. Physical activity is generally considered to be an environmental factor that affects bone mineral density throughout life. However, the process of bone response to mechanical load and its molecular mechanism remain largely unknown. The mechanosensitive cation channel protein Piezo l serves as a response target for mechanical stimulation of mesenchymal stem cells, osteoblasts, osteoclasts, and chondrocytes, providing a new idea and direction for mechanical load stimulation (exercise) to improve bone metabolism. [ABSTRACT FROM AUTHOR]

Objective To investigate the effect of serum uric acid (SUA) on prognosis of patients with maintenance hemodialysis (MHD) complicated with mineral metabolism disorder. Methods A total of 192 patients with MHD complicated with mineral metabolism disorder were selected. According to the level of SUA, patients were divided into the group Q1 (SUA≤363 μmol/L, n=47), the group Q2 (SUA 364-459 μmol/L, n=48), the group Q3 (SUA 460-559 μmol/L, n= 49) and the group Q4 (SUA≥560 μmol/L, n=48). The clinical data were compared between the 4 groups. Patients were followed up. Spearman method was used to analyze the correlation between SUA and other index. Kaplan-Meier method was used to compare the survival rates of the 4 groups, and Cox regression model was used to analyze the effect of SUA on the prognosis of the patients. Results Compared with the group Q1, serum creatinine (Scr), urea nitrogen (BUN) and blood phosphorus (P) were increased in the groups Q2, Q3 and Q4, glomerular filtration rate (eGFR) and high density lipoprotein (HDL-C) were decreased, and albumin (ALB) was increased in the groups Q2 and Q4 (P＜0.05). Compared with the groups Q2 and Q3, the levels of Scr, BUN and P were decreased in the group Q4 (P＜0.05). SUA was positively correlated with ALB, BUN, Scr, P, and magnesium (Mg), and negatively correlated with calcium (Ca) (P＜0.05). The mean follow-up time was 16.0 (11.0, 23.8) months. A total of 45 patients (23.4%) had outcome events, including 22 patients (11.5%) with cardiovascular death. Kaplan-Meier survival curve showed that the all-cause mortality and cardiovascular disease mortality were higher in the group Q1 (Log-rank χ2 =9.707 and 7.912, P＜0.05). Univariate Cox regression analysis showed that low ALB, low blood Mg, and CVD were risk factors for cardiovascular disease death in MHD patients with mineral metabolism disorder, while higher uric acid (the Q4 groups) was its protective factor (P＜0.05). Multivariate Cox regression analysis showed that advanced age, low blood Mg and cardiovascular disease were independent risk factors for all-cause death in MHD patients with mineral metabolism disorder, while higher uric acid (the groups Q3,Q4) was its protective factor (P＜ 0.05). Conclusion Higher SUA can reduce all-cause mortality in MHD patients with mineral metabolism disorders. [ABSTRACT FROM AUTHOR]

Objective: To evaluate the relationship between nap habit and cognitive function in the retired elderly. Methods: Totally 835 elderly people [average age (69. 8 ± 6. 8) years] were selected from 3 main urban areas of Beijing. According to the age stage of starting nap, they were divided into 4 groups, namely no nap group, middle-aged nap group, old napping group and change group (middle-aged nap but not for the elderly). The Montreal Cognitive Assessment (MoCA) and Neuropsychological Test Battery (NIB), including auditory word test, associative learning test and Wechsler mapping test, were used to measure cognitive function. Results: The scores of association learning were higher in middle-aged nap group than in elderly nap group (P < 0. 05) . Multivariate linear regression analysis showed that different nap habits of middle-aged and elderly nap groups negatively predicted the score of associative learning (β = - 0. 32). Conclusion: It suggests that the people who start to take naps at the middle age are better at association learning than the people who start napping at the elderly age. [ABSTRACT FROM AUTHOR]

Objective To study the efficacy and safety of YiQiShengSui (YQSS) formula, a traditional Chinese medicine (TCM) herbal prescription, on neutropenia of epithelial ovarian cancer patients caused by chemotherapy. Methods This was a prospective randomized controlled non-blind clinical study. A total of 60 patients from Obstetrics and Gynecology Hospital of Fudan University were randomly assigned to the research group and the control group with a ratio of 1∶1. During the intermission of chemotherapy, the patients in the control group took recombinant human granulocyte stimulating factor injection (rhG-CSF), the dose of rhG-CSF was 150 μg i.h. q6-24h, according to grade of neutropenia. The patients in the research group took YQSS formula for 21 days along with rhG-CSF in the same period.The changes of white blood cells (WBC), absolute neutrophil count (ANC) of the next chemotherapy, as well as the changes on the 7th day and 10th day after this chemotherapy, duration of chemotherapy interval, treatment time of leukocyte elevation and dosage of rhG-CSF, as well as the hepatic and renal function, and quality of life scale (FACT-G) were observed for safety and efficacy evaluation. Results The levels of WBC and ANC in the research group were higher than those in the control group on the 7th and 10th days after chemotherapy, with statistical significance (P<0.05). Further Logistic multivariate regression analysis showed that YQSS was a significant correlation factor for alleviating moderate and severe ANC reduction on the 10th day after this chemotherapy.The interval time of chemotherapy in the research group and the control group was (22.31±1.25) days and (25.79±4.76) days, respectively, and the treatment time of leukocyte elevation was (4.67±2.11) days and (6.86±2.35) days, respectively, the differences were statistically significant (P<0.05) .The dosage of rhG-CSF in the research group and the control group was (552.6±173.6) μg and (852.6±200.3) μg, respectively, and the difference was statistically significant (P<0.05). There was no statistically significant difference in hepatic and renal function damage between the two groups.The total FACT scale score, physiological status score, affective status score and functional status score of the research group were higher than those of the control group (all P<0.05). Conclusion YQSS combined with rhG-CSF can effectively increase white blood cells, shorten the time between chemotherapy and the treatment time of leukocyte elevation, reduce the dosage of rhG-CSF, and improve the quality of life of patients.The use of YQSS did not increase the damage of hepatic and renal function of patients. [ABSTRACT FROM AUTHOR]

The thrust force generated by drilling of Carbon Fiber Reinforced Plastic(CFRP)is the main factor causing machining damage. In order to study the influence of machining parameters on the trust force of drilling, a three-dimensional drilling model of CFRP is established, the VUMAT subroutine of three-dimensional Hashin failure criterion and damage evolution criterion is written by applying Fortran language, and the influence of spindle speed and feed amount on the trust force is examined by orthogonal test, and the trust force prediction model is established. The results show that the trust force is significantly affected by the spindle speed and feed amount, and the trust force decreases with the increase of spindle speed and increases with the increase of feed amount; the maximum relative error of the established multiple linear regression prediction model is 11.3%,and the maximum relative error of the quadratic multiple regression prediction model is 4.8%. [ABSTRACT FROM AUTHOR]

Objective To observe the changes of miR-92b-5p and high-mobility histone Box1(HMGB1)in serum of patients with type 2 diabetic nephropathy and to explore its clinical significance. Methods Two hundred and three patients with type 2 diabetes mellitus were selected and divided into the simple diabetes group(n = 98)and the diabetic nephropathy group(n = 105)according to whether they were complicated with nephropathy. Eighty healthy people in the same period were included as the control group. Enzyme linked immunosorbent assay(ELISA)was used to detect serum HMGB1, real-time PCR was used to detect serum mir-92b-5p, multivariate Logistic regression was used to analyze the risk factors of type 2 diabetic nephropathy, Pearson test was used for correlation analysis, and ROC curve was used to evaluate the predictive value of miR-92b-5p and HMGB1 for type 2 diabetic nephropathy. Results The levels of serum HMGB1 in the simple diabetes group and the diabetic nephropathy group were higher than those in the control group, and the level in the diabetic nephropathy group was higher than that in the simple diabetes group(all P < 0. 05). The levels of miR-92b5p in the simple diabetes group and the diabetic nephropathy group were lower than those in the control group, and the level of miR-92b-5p in the diabetic nephropathy group was lower than that in the simple diabetes group(all P < 0. 05). Multivariate Logistic regression analysis showed that the increase of serum HMGB1 level and the decrease of miR-92b-5p level were independent risk factors in the progression of type 2 diabetes mellitus to type 2 diabetes nephropathy(all P < 0. 05). The level of serum miR-92b-5p was negatively correlated with serum HMGB1 and 24 h urinary protein(24 hpro), and positively correlated with estimated glomerular filtration rate(eGFR)in patients with type 2 diabetes mellitus(all P < 0. 05). Serum HMGB1 was positively correlated with 24 hpro, and negatively correlated with eGFR(all P < 0. 05). The area under the ROC curve(AUC)of serum HMGB1 combined with miR-92b-5p in predicting type 2 diabetic nephropathy was 0. 858(95% CI: 0. 800-0. 908), which was higher than that of miR-92b-5p(AUC = 0. 754, 95% CI: 0. 688-0. 821)and serum HMGB1(AUC = 0. 802, 95% CI: 0. 737-0. 860)(all P < 0. 05). Conclusions The increase of serum HMGB1 and the decrease of serum miR-92b-5p are the risk factors for the development of type 2 diabetes mellitus to diabetic nephropathy. Early combined detection of two indicators can be used as important biochemical markers for clinical prediction and diagnosis of type 2 diabetic nephropathy. [ABSTRACT FROM AUTHOR]

An analytical method of the chiral stationary phase/high performance liquid chromatographytandem mass spectrometry technique coupled with QuEChERS for determination of the enantiomers of profenofos in citrus was developed, and the selective degradation behavior of profenofos enantiomers in citrus was studied through field experiments. The sample preparation involved quick extraction with acetonitrile and cleaning-up with multi-walled carbon nanotube sorbent. The enantiomers of profenofos were separated by a ND (2)-RH chiral column, detected by tandem mass spectrometry in multi-reaction monitoring (MRM) mode, and quantified using matrix-matched calibration curves. The results showed good linear relationships for the analytes in the concentration range of 2.5-250 μg/L with their correlation coefficients (r) >0.999 6. The limits of quantitation (LOQs) were 5 μg/kg. The average recoveries of the two enantiomers of profenofos in citrus ranged from 76% to 83% at three spiked levels(5, 100, 500 μg/kg) and the relative standard deviations (RSD) were between 2.6% and 6.0%. Field experiments were conducted to study the differences in the degradation of profenofos enantiomers in citrus. The results of dynamic experiments showed that degradation of the enantiomers of profenofos in citrus conformed to the first-order reaction kinetic equation, and the half-lives of (+)-profenofos and (−)-profenofos were 12.53 d and 14.14 d, respectively. The enantiomer fraction (EF) value of the profenofos enantiomers in the field experiments reached 0.43 after 30 d of application, indicating that there were significant enantio-selectivity in the degradation of profenofos enantiomers in citrus. [ABSTRACT FROM AUTHOR]

OBJECTIVE: Using quality control circle management tools to reduce the number of irrational prescriptions and improve the quality of prescriptions in outpatient. METHODS: Research data came from a total of 714 458 outpatient prescriptions in the People’ s Hospital of Banan District in Chongqing from Jan. to Oct. 2020,of which 5 482 were irrational prescriptions. Quality control circle activity was carried out in outpatient pharmacy to screen out irrational outpatient prescriptions in the former 5 months of the activity( Jan. to May 2020) and conduct status investigation, cause analysis, formulating and implementation of countermeasures to solve the problems, and performed comparative analysis on the changes of the number of irrational prescriptions in outpatient before and after the quality control circle activity. RESULTS: In the later 5 months of the quality control circle activity(Jun. to Oct. 2020),the incidence of irrational outpatient prescriptions had decreased from 28. 42 prescriptions/ d in the former 5 months of the quality control circle activity( Jan. to May 2020) to 8. 13 prescriptions/ d,with the amplitude was 71. 39%,and the number of irrational prescriptions was significantly reduced. CONCLUSIONS: The application of quality control circle management methods can effectively reduce the irrational rate of outpatient prescriptions,promote rational drug application in hospital and ensure the safety of patients’ medication. [ABSTRACT FROM AUTHOR]

Objective To explore the expression of MAP4K1 in melanoma cell lines and tissues and the effect of MAP4K1 on the migration and invasion of melanoma cell lines and molecular mechanism. Methods Fifty-four patients with melanoma admitted to the Department of Dermatology of Chongqing Traditional Chinese Medicine Hospital from January 2010 to December 2015 were selected. The expressionof MAP4K1 in melanoma was analyzed by online database. The expression of MAP4K1 mRNA and protein in melanoma was detected by qRT-PCR and immunohistochemistry. The MAP4K1 gene knockout was detected by Western blot and RT-PCR. The effecton migration and invasion of A375 cells after MAP4K1 gene knockout was detected by scratch assay and Transwell assay. The regulation of YAP/TAZ by MAP4K1 was detected by dual luciferase reportergene assay. The correlation between MAP4K1 and the expression of downstream target of Hippo-YAP pathway was proved by GEPIA online database. The effect on Hippo-YAP signaling pathway after MAP4K1 gene knockout was verified by Westernblot. Results Compared with the adjacent tissues, the expression of MAP4K1 mRNA and protein in cutaneous malignant melanoma cells and tissues was up-regulaged (P<0.001). The migration and invasionof A375 cells after MAP4K1 gene knockout were significantly inhibited (P<0.001). The transcription activity of Yap/TAZ after MAP4K1 gene knockout and downstream target molecules were down-regulated. The Hippo-YAP signaling transduction pathway was inhibited by MAP4K1 gene knockout. Con原 clusion The expression of MAP4K1 in melanoma is up-regulated. The MAP4K1 can be served as potential oncogene to promote the development of melanoma by regulating Hippo-YAP pathway. [ABSTRACT FROM AUTHOR]

The topology and routing algorithm of network on chip ( NoC) directly influence the transmission delay and the transmission efficiency of the network-on-chip. A new deadlock-free routing algorithm for NoC was proposed based on 2D-Torus topology. By changing the position of packets that are restricted to tum during NoC routing, the adaptive routing condition of network-on-chip was guaranteed, and the delay of network-on-chip was reduced. The 2D-Torus NoC based on this routing algorithm was designed and implemented on FPGA hardware platform, and then was tested. The experimental results indicated that the NoC based on this routing algorithm can meet the performance requirements of network-on-chip data communication for multi-direction and multi-channel. [ABSTRACT FROM AUTHOR]

瘢痕疙瘩是由皮肤在组织损伤修复过程中形成的纤维细胞异常增殖、胶原过量堆积导致的。耳垂部作为瘢痕疙瘩 的好发部位，常继发于穿耳孔、烧伤或外伤，临床上表现为超过伤口边缘的持续性瘤状瘢痕增生，不但会影响耳部美观，同时还 影响患者的心理健康。由于瘢痕疙瘩的发病机制相对复杂，且目前尚未完全明确，临床上的治疗方法也各不相同。针对耳垂瘢痕 疙瘩采取传统单纯手术切除的方式不但效果不理想，而且有较高的复发率。目前，采用手术切除联合放疗、局部注射药物、冷冻、 压力治疗以及激光等方法是治疗耳垂瘢痕疙瘩的较有效措施。 [ABSTRACT FROM AUTHOR]

Objective: To investigate the clinicopathological significance and prognostic value of Kallistatin expression in breast cancer. Methods: The wax mass and clinical data of breast cancer archives were collected and divided into primary non-lymph node metastasis (NMBT), primary lymph node metastasis (PBT) and matched lymph node metastasis (PMLN). The expression of Kallistatin was detected by immunohistochemical technique and analyzed statistically. Results: The results showed that the expression of kallistatin in primary lymph node metastasis was higher than that in primary lymph node metastasis without lymph node metastasis and matched lymph node metastasis. The expression of kallistatin was significantly correlated with histological type (P=0.003), lymph node status (P< 0.001), clinical stage (P=0.002), estrogen receptor (ER) expression (P=0.046). The positive expression rate of kallistatin in invasive lobular carcinoma was higher than that in invasive ductal carcinoma. The positive expression rate of kallistatin in breast cancer with lymph node metastasis was significantly higher than that in breast cancer without lymph node metastasis. Kaplan-Meier analysis showed that the positive expression of kallistatin was a risk factor for short disease-free survival (P=0.008) and short overall survival (P=0.006) in breast cancer patients. In patients with invasive ductal breast cancer, the positive expression of kallistatin was associated with short survival time (P=0.026). It was also related to the short survival time of ER positive patients (P=0.010). Conclusion: The expression of Kallistatin in breast cancer has more complicated clinicopathological significance, and its expression suggests poor prognosis. [ABSTRACT FROM AUTHOR]

A library of π-conjugated systems have been developed through conventional tools of coupling (e.g. Suzuki coupling, Stille coupling) and have been used for the fabrication of organic optoelectronic devices. Herein, a facile, atomefficient and environmentally benign pathway is outlined, with water as the only by-product, for the synthesis of conjugated Schiff-based copolymers based on sulfur-heterocycle fused naphthalene diimides (NDIs) and aromatic diamines. These copolymers showed broad absorption spectra in the ultraviolet-visible (UV-Vis) region and low-lying LUMO levels at about −4.07 — −4.19 eV. The relationship between the chemical structure and the performance, including the optical properties, electrochemical properties, thermal stability, morphologies and the field-effect performance in transistors, is fully studied using these polymers as active layer. Compared with P1 and P2, P3 exhibits an absorption peak at 754 nm, with red shifts of 76 nm and 75 nm, respectively. An electron mobility of up to 2.47×10−3 cm2/ (V·s) can be achieved for P3-based transistors after performing a thermal annealing process at 300 ℃. The incorporation of rigid structure units into polymer conjugated skeleton can effectively tune its spectral absorption, energy level structure as well as the film morphology, thus improving the device performance. [ABSTRACT FROM AUTHOR]

In order to decrease the inertial force of connecting rod of emulsion pump under the high-pressure dynamic load, the Kane method is used to establish theoretical dynamical model of the three-plunger emulsion pump, and substitute relevant dimensions of the three-plunger emulsion pump, and deeply study the change of inertial force of pump.With the help of MATLAB software, the curve of the inertial force of the connecting rod that changes with the rotation time of crank is drawn. The SolidWorks model is imported into the ADAMS software for dynamic simulation. The results show that the ADAMS simulation analysis results are basically consistent with the theoretical model established by the Kane method,and the correctness of the method is verified. Based on the Kane method, the impact of the length ratio of the crank to the connecting rod, called λ, the cranking angular velocity,called ω,on the inertial force of the connecting rod are analyzed. The results show that when length of crank is constant, increased λ, inertial force increases.This is because λ has a greater impact on mass than acceleration of mass center. The angular velocity of crank which is called ω increases, the inertial force of connecting rod oscillates, and the amplitude of the oscillation becomes larger and larger, which is deduces that the oscillation frequency increases as well.According to the results, increasing the λ appropriately and decreasing ω could reduce the inertial force of the connecting rod and improve the working performance of the connecting rod. [ABSTRACT FROM AUTHOR]

To analyze the common clinical examination and therapeutic effect of influenza A virus with pulmonary infection by comparing with non-viral pulmonary infection, so as to provide a basis for empirical treatment of influenza A virus. 25 patients with confirmed influenza A virus infection with lung infection (group A) and 25 patients with non-viral pulmonary infection (group B) were selected in our hospital from November 2017 to April 2018. Patients with confirmed influenza A were given oseltamivir antiviral therapy for one week, and both groups were given routine antiviral therapy. The inflammation indexes such as white blood cell count, neutrophil ratio, C-reactive protein and treatment day, hospitalization day, symptoms disappearing time and adverse reactions were compared between the two groups. There was no significant difference in CT findings between the two groups (P>0.05). The white blood cell count in group A was significantly lower than that in group B (P<0.05). There was no significant difference in neutrophil ratio and C-reactive protein level between the two groups (P>0.05). There was no significant difference in the treatment days and hospitalization day between the two groups (P>0.05). There was no significant difference between the two groups in the body temperature returned to normal, cough disappear time, disappearance time of lung rales and shortness of breath(P>0.05). The incidence of adverse reactions was 16.00% (4/25) in group A and 12.00% (3/25) in group B, There was no significant difference between the two groups (P>0.05). Influenza A virus with pulmonary infection is often manifested as normal blood routine, often accompanied by varying degrees of inflammation, in addition to conventional anti-infective treatment, oral oseltamivir can promote the prognosis of the disease. [ABSTRACT FROM AUTHOR]

BACKGROUND: Abnormal phosphoinositide 3-kinase and protein kinase B occurs in insulin signaling transduction of type 2 diabetes, and improvement in insulin sensitivity post-exercise has been shown to be related to the increased protein expression and activity in insulin signaling transduction. OBJECTIVE: To investigate the effect of 8-week aerobic exercise on the phosphoinositide 3-kinase-protein kinase B signaling pathway in type 2 diabetic skeletal muscle of middle-aged rat models. METHODS: Seventy-five 10 month-old female Sprague-Dawley rats were provided by Chengdu Dashuo Experimental Animal Co., Ltd., and the study was approved by the Research Ethics Committee of Shenyang Sport University, approval number: 2015006. The rats were randomized into five groups: normal control group (common diet), diabetes control 1 group (8-week high fat diet prior to injection of 35 mg/kg streptozotocin; continue to give high fat feed to 16 weeks), diabetes exercise 1 group (8-week high fat diet prior to injection of 35 mg/kg streptozotocin+ aerobic exercise; continue to give high fat feed to 16 weeks), diabetes control 2 group (16-week high fat diet prior to injection of 35 mg/kg streptozotocin), and diabetes exercise 1 group (16-week high fat diet prior to injection of 35 mg/kg streptozotocin+ aerobic exercise). The aerobic exercise was undergone at the 9th week. The blood lipid, blood glucose, insulin and gastrocnemius phosphoinositide 3-kinase-protein kinase B expression levels were tested after 8 weeks of exercise. RESULTS AND CONCLUSION: (1) Both high-fat and high-fat diets at 8 and 16 weeks combined with low-dose streptozotocin could induce the decrease of phosphoinositide 3-kinase and protein kinase B protein expression in the rat skeletal muscle, and impaired phosphoinositide 3-kinase-protein kinase B signal transduction caused lipid and glucose metabolism disorders, decreased insulin sensitivity, and induced type 2 diabetes. (2) Compared with the diabetes control groups, the body mass in the aerobic exercise groups was on a rise, fat mass and body fat rate were on a descent, triacylglycerol, total cholesterol, free fatty acid and low density lipoprotein were significantly decreased, fasting blood-glucose and fasting insulin levels were significantly decreased, and activities of phosphoinositide 3-kinase and protein kinase B were significantly increased. (3) These results indicate that exercise intervention before and after inducing diabetes can reverse/enhance the phosphoinositide 3-kinase-protein kinase B phosphorylation level in skeletal muscle, increase insulin sensitivity, improve lipid metabolism and disorder of glucose metabolism, and ultimately effectively improve and prevent the occurrence and development of type 2 diabetes. [ABSTRACT FROM AUTHOR]

Objective: Expression and clinicopathological significance of S100 calcium binding protein A14(S100A14) in different molecular subtypes of breast cancer were explored to provide reference for identifying new molecular typing markers. Methods:Breast cancer tissues were taken cases for radical mastectomy patients from January 16, 2013 to 22 May 2014 at the Cancer Hospital Affiliated to the Xiangya Medical College of Central South University & the Cancer Hospital of Hunan province. Using immunohistochemistry to detect the expression of S100A14 in 254 breast cancer tissues, with emphasis on differences expression of the protein in different molecular subtypes of breast carcinoma and its correlation with clinicopathological factors, Kaplan-Meier analysis was applied for the relationship between S100 A14 expression and prognosis of patients with breast cancer. Results: The positive expression of S100 A14 in four molecular subtypes of ER+/PR+/HER2+, ER+/PR+/HER2-, ER-/PR-/HER2+ and ER-/PR-/HER2-breast cancer was 38.5%, 47.1%, 75.5%, 80%, respectively. The expression of the 4 groups was the highest in the ER-/PR-/HER2-type and the lowest in the ER+/PR+/HER2+ type, and the difference between the 4 groups was significant(x2=32.997, P <0.001). The relationship between S100A14 and clinicopathological characteristics of 254 cases of breast cancer was analyzed. The expression of S100A14 was significantly negatively correlated with the expression of ER and PR(all r=-0.353, P<0.001), and was significantly positively correlated with postoperative recurrence of liver metastasis with breast cancer(r=0.134, P=0.040). There is no significant correlation between the expression of S100A14 and the clinicopathological features of type ER+/PR+/HER2+ and ER+/PR+/HER2-type breast cancer(P>0.05).In ER-/PR-/HER2+ type breast cancer. The positive expression rate of S100 A14 in axillary lymph node metastasis group was significantly higher than that without axillary lymph node metastasis group(x2=6.768, P=0.013), and S100A4 expression has a negative correlation with postoperative pulmonary metastasis(r=-0.272, P=0.044). Conclusion: The expression of S100A14 in breast cancer with different molecular subtypes is different, and its high expression is associated with metastasis or recurrence of breast cancer with different molecular types. It may be used as a candidate marker for molecular typing of breast cancer. [ABSTRACT FROM AUTHOR]

目的探讨医护一体化护理模式在桃体切除术患儿术后护理中的应用效果。方法选取2019年5月至2020 年5月该院收治的200 例行扁桃体切除术的患儿,按照抽签法随机分为对照组和观察组,各100例。对照组采用常规术后护理, 观察组在对照组基础上行医护一体化护理干预,比较2组患儿术后视觉模拟评分法(VAS)评分,切口愈合时间、住院时间及并发 症发生率,结果观察组患儿术后下3、7d 时VAS评分及切口愈合时间、住院时间、并发症发生率均优于对照组,差异有统计学 意义(P<0.05)。结论医护一体化护理模式在小儿病桃体切除术后护理中的应用效果较理想,可缓解悉儿疼痛,促进其及早康 复,同时可降低术后并发症发生风险。 [ABSTRACT FROM AUTHOR]

In order to solve the problem that the process of cross and mutation in traditional genetic algorithm is too cumbersome， and the extreme value judgment and the convergence rate is limited， a parallel quantum genetic algorithm(QGA) is proposed to optimize the weights of the neural network. The concept of quantum computing is firstly introduced. In the process of quantum computation， the quantum rotation gate is used to train the chromosomes. Then the quantum cross is introduced to overcome the precocious convergence and to avoid the cumbersome cross and mutation process in the genetic algorithm. Finally， the parallel convolution neural network is designed and implemented. The parallel quantum genetic algorithm is used to optimize the weights of the convolution neural network， and a facial recognition system based on parallel quantum genetic neural network is realized. Experimental results show that compared with the original genetic algorithm， the quantum genetic neural network algorithm has obvious improvements in terms of robustness and processing speed. [ABSTRACT FROM AUTHOR]

Objective To explore the effect of Chinese traditional fitness Qigong, Yi Jinjing, Five-animal exercise, and Eight section brocade on bone mineral density（BMD） of the lumbar spine and proximal femur in elderly women. Methods The age, height, and weight were matched among Yi Jinjing group（n=22）, Five-animal exercise group（n=22）, Eight section brocade group（n=21）, and control group（n=25） in the study. The exercise lasted for 24 weeks（5 days/week, 70 minutes/time）. BMD of the L2-4 and the proximal femur of the 4 groups were tested. Results After 24 weeks, BMD of lumbar L2-4 in Yi JinJing group and Eight section brocade group increased by 12% and 11%, respectively（P<0.05）. BMD of the L2-4 in the lumbar vertebra of Five-animal exercise group increased by 5.6%, and there was no significant change（P>0.05）. BMD of lumbar spine L2-4 in Yi JinJing group and Eight section brocade group were significantly higher（12% and 9.8%, respectively） than that in control group（P<0.05）. Conclusion Yi Jinjing and Eight section brocade exercise increase the BMD of the lumbar L2-4 in elder women. The effect of three kinds of Qigong on BMD of the proximal femur is not obvious. [ABSTRACT FROM AUTHOR]

Subsided mined-land reclamation with Yellow River Sediments is an effective way and a win-win strategy for reducing the Yellow River sedimentation and accelerating mined land reclamation by using sediments as filling materials, which is of great significance to improve the regional ecological environment. In order to analyze the maximal supply amount and sediments demand for the filling reclamation of mining areas in the Yellow River basin, in this paper, we took the Yellow River and 23 national planning mining areas within 150 km away from the main sediments deposition segments of the Yellow River as the study area. Through the analysis of supply and demand of sediments for the filling reclamation of mining areas, the river segments and mining areas suitable for filling reclamation were preliminarily identified and the schematic sediments transport paths were also designed. Firstly, the sediments balance equation was used to estimate the sedimentation amount in main sediments deposition segments of the Yellow River in 1950－2013. Secondly, the sediments demand for filling reclamation of all mining areas within the study area was estimated. Based on the above two steps, we analyzed the spatial difference on the relation of Yellow River sediments supply and demand for the filling reclamation in all main sediments deposition segments. Finally, the sediments transport paths of different mining areas were designed as a whole by following the principle that the path should be shortest for minimum construction and labor costs involved. The results showed that the main sediments deposition segments of the Yellow River are Bayangol－Toudaoguai and Mengjin－Lijin, whose total sedimentation amount was 14.431 billion tons in 1950 －2013. Therefore, the sedimentation was the most serious in the river segment called Mengjin－Gaocun, where the accumulated sedimentation amount was 11.475 billion tons in 1950－2013. By the distance difference from the Yellow River, those 23 mining areas were further divided into three types of mining groups, including nine short-distance (S1) mining areas, 10 middle-distance (S2) mining areas, and four long-distance (S3) mining areas, which were 0 - 60 km, >60 - 120 km and >120 - 150 km away from the Yellow River, respectively. Their total sediments demand amount was about 22.512 billion tons by 2013. The spatial relationship between the supply and demand of the Yellow River sediments was unbalanced, thus not all mining areas could be reclaimed with Yellow River Sediment for insufficient sediment in some river segments. In the river segments including Bayangol－Sanhuhekou, Huayuankou－Gaocun, Aishan－Lijin, the sedimentation amount could cover the reclamation demand, thus all the mining areas could be filling-reclaimed using the sediments only judging by the difference between the sediment supply and reclamation demand, but in the river segments including Sanhuhekou－Toudaoguai, Mengjin－Huayuankou and Gaocun－Aishan, the sedimentation amount was less than the reclamation demand, thus only part of the mining areas could be suitable for the sediment-filling reclamation application. As a result, 7 S1 mining areas and 5 S2 mining areas, including Baotou, Yima, Jiaozuo, Zhengzhou, Feicheng, Huang Hebei, Zibo, Wuhai, Pingdingshan, Jincheng, Hebi and Xinwen, were comparatively suitable for filling reclamation by both sediments transportation length and sediments sufficiency or not, whose total sediments demand was 4.531 billion tons around and 4.488 billion tons, respectively by 2013, about 9.019 billion tons in total, accounting for about 40.06% of the total sediments demand of all mining areas and about 62.50% of the total sedimentation amount in the study area. The total water involved for sediment transportation to the mining areas suitable for filling reclamation was about 14.881 billion tons by 2013. The sediments transport paths length of the mining areas suitable for filling reclamation was about 21.98 - 109. 02 km away from the Yellow River. The total sediments transport paths length to the suitable mining areas was about 643 km. The study is the foundation for the wide-scale promotion of the filling reclamation technology of mining areas with Yellow River sediments, and is helpful for the subsequent feasibility study, the relevant planning and reclamation policies making. [ABSTRACT FROM AUTHOR]

Objective To evaluate the clinical efficacy and safety of transjugular intrahepatic portosystemic shunt (TIPS) versus endoscopic cyanoacrylate injection in the treatment of gastric variceal bleeding through a meta-analysis. Methods PubMed, Embase, the Cochrane Library, ScienceDirect, CNKI, and Wanfang Data were searched for the randomized controlled trials (RCTs) of the efficacy of TIPS versus endoscopic cyanoacrylate injection in the treatment of gastric variceal bleeding published up to June 30, 2018. We assessed study quality and extracted data from the included studies. RevMan 5.3 software was used to statistically analyze the data. Results Six RCTs were included, involving 366 cases in the TIPS group and 255 cases in the cyanoacrylate group. The meta-analysis showed no significant difference in the early rebleeding rate between the TIPS group and the cyanoacrylate group (odds ratio ［OR］=1.05, 95% confidence interval ［CI］: 0.53-2.06, P=0.89), but the postoperative late rebleeding rate of the TIPS group was significantly lower than that of the cyanoacrylate group (OR=0.33, 95%CI: 0.19-0.57, P＜0.000 1). There was no significant difference in the mortality between the TIPS group and the cyanoacrylate group (OR=1, 95%CI: 0.65-1.55, P＞0.05). The TIPS group had a significantly increased incidence of postoperative hepatic encephalopathy compared with the cyanoacrylate group (OR=4.09, 95%CI: 1.59-10.58, P=0.004). Conclusions In the long term, the rebleeding rate of gastric variceal bleeding is significantly lower in patients treated with TIPS than in those treated with endoscopic cyanoacrylate injection, so TIPS is an effective method to treat gastric variceal bleeding. [ABSTRACT FROM AUTHOR]

In view of the problems of indoor air pollution in modern buildings, high power consumption of indoor air purifier and no outdoor air exchange, a new two-way air purifying and ventilating device was designed and developed based on Pro/E three-dimensional structure modeling technology.Through the finite element modal analysis, it is considered that the material can be optimized, and the air-fluid calculation and simulation can be carried out by using Fluent software to compare the fluid parameters before and after the optimization. The results show that the elastic modulus has a significant influence on the first-order frequency of the device, and that the first-order frequency is reduced from 63.3Hz to 37.1Hz after the carbon fiber composite material is replaced by aluminum alloy material in the external part of the device which meets the non-resonance condition. The fluid parameters decrease slightly compared with those before optimization, and the number of ventilation per hour decrease from 4.66 times/h to 4.38 times/h, which still accords with the national standards. [ABSTRACT FROM AUTHOR]

Objective To study the effects of different paraquat concentrations on the autophagy of human bronchial epithelial cells （16HBE） ．Methods 16HBE was cultured with the different concentrations （50 μM， 150 μM，500 μM） of paraquat，respectively in 0 h， 12 h，24 h. LC3-Ⅱ，LC3-I and P62 protein expressions were measured by Western Blot in the cell. The expressions of autophagosome in the poisoning group and control group were observed by electron microscopy．Results In the experiment， a large number of autophagosomes appeared in cytoplasm in the PQ poisoning group， and the number of autophagosomes was significantly increased compared with the control group．LC3-II/LC3-I ratio in the 50 μMPQ poisoning group was the highest in the 24-hour group，The 12-hour group was lower than 24-hour one，while the expression level of the control group was the lowest（ < 0.05），P62 protein concentration was the lowest in 24 hours， followed by the 12-hour group， and the expression level of the control group was the highest（ < 0.05） ．Western Blot detection of LC3-II/LC3-I ratioand P62 protein in the 150， 500 μMPQ poisoning group was not statistically significant compared with the control group．Conclusion The autophagy adjustment effected by PQ depends on human bronchial epithelial cells. [ABSTRACT FROM AUTHOR]

Objective: To investigate the expression and clinicopathological significance of cysteine peptidase 7(Caspase-7,CASP7) in different molecular subtypes of breast cancer. Methods: The expression of CASP7 in 254 cases of breast cancer tissues were detected by using immunohistochemistry, the differences in CASP7 expression in different molecular subtypes of breast carcinoma and its correlation with clinicopathological factors were analyzed, Kaplan-Meier analysis was performed to analyze the relationship between CASP7 protein expression and prognosis of patients with breast cancer. Results: The positive expression rates of Caspase-7 in ER+PR+HER2+, ER+PR+HER2-, ER-PR-HER2+, ER-PR-HER2-were 37.2%, 60.3%, 17.0%, 40.0% respectively, which was strongest in ER+/PR+/HER2-and lowest in ER-/PR-/HER2+.(P<0.001). Caspase-7 expression was positively correlated with the expression of ER and PR(r=0.194, P=0.002), and was negatively correlated with the expression of HER2(r=-0.224, P<0.001). In ER-PR-HER2+, CASP7 expression was related to tumor diameters(P=0.028) and postoperative mediastinal metastasis and brain metastasis(r=0.307, P=0.026). In this study, there was no relation between CASP7 expression and survival status of breast cancer patients. Conclusion: The expression of CASP7 in different molecular subtypes of breast cancer was different and had a certain clinical and pathological significance. It may be a candidate marker for the molecular classification and prognostic prediction of breast cancer. [ABSTRACT FROM AUTHOR]

How to determine the ecological impact boundary of coal mining is one of the difficulties in the research field of land ecology in mining areas. For a long time, surface subsidence depth of 10 mm is generally used as the coal mining disturbance boundary not only to the developed land but also to the vegetation-covered land in the academic research and planning practice. Land reclamation is still bounded by the subsidence contour with the expected surface subsidence depth of 10 mm as the boundary. In fact, many scholars and other professionals have realized that 10 mm sinking is not adaptable as the boundary of mining influence on land ecology. Our research goal was to find a remote sensing method to identify mining impact boundary, which could be used to evaluate ecological accumulating effect of coal mining on vegetated area. Yanzhou coal field, a typical coal mine area with high groundwater level in the eastern China, was taken as the study area, where the coal mining has caused a lot of impounded water areas, and the spatial distribution characteristics of the temperature vegetation drought index (TVDI), which is linear with soil moisture, were analyzed. Then the influence boundary of mining subsidence on soil moisture was determined, and the difference between the influence boundary using TVDI spatial changing tendency and the expected surface subsidence of 10 mm was analyzed. Firstly, the authors calculated TVDI and found it was mainly concentrated in the range of 0.2-0.6, which meant soil moisture levels were mainly “normal” and “slight drought”. The areas belonging to “normal” level and “slight drought” accounted for 45.17% and 40.09% of the whole study area respectively. Secondly, the authors tried to obtain the influence boundary of soil moisture and analyzed the spatial distribution characteristics of TVDI from the edge of the impounded water area by mining subsidence. The impounded water areas i.e. A, B, C, D, F, G and H were taken as the research objects and the different distance ranges from the edge of the impounded water area were divided. With the increase of the distance from the edge of the impounded water area, the median TVDI value increased and then tended to be stable. Due to the differences in coal seam, mining methods and processes, the influence range of coal mining on soil moisture varied in different subsided areas. The authors proposed an exponential model to identify the mining influence boundary, in which the value of asymptotic line was defined as the disturbed boundary. Study results showed that TVDI stable value in each impounded water area is between 0.38 and 0.43. Fitting TVDI value with exponential function, it could be found that the correlation coefficients are greater than 0.60, and the mean square root errors are less than 0.02. The impounded water area H is fragmented, irregular and has strong internal spatial interaction, which has the largest influence distance reaching 781 m. Finally, the ecological disturbance range of coal mining based on TVDI extraction method was expected to be smaller than subsidence depth boundary, namely 10 mm. The paper’s innovation is to propose a new method to identify the mining influence boundary, which is the theoretical boundary is the asymptotic line of ecological index changing from the edge of impounded water area to the unmined area by increased disturbance range. [ABSTRACT FROM AUTHOR]

This paper investigates Yanzhou coalfield which locates in Eastern China with high groundwater level.Landsat TM images are used to extract the land surface temperature using the single window algorithm. The spatial distribution of land surface temperature is analyzed from four aspects including land use type,vegetation coverage, the distance from the edge of water-impounded subsided-area and the subsidence depth. The results show that the land surface temperature varies greatly among different land use types; the developed land and the water area have the highest and the lowest average surface temperature respectively; land surface temperature is negatively correlated with vegetation coverage; land surface temperature decreases with the increase of the distance from the edge of water-impounded subsidedarea and then becomes stable by exponential function fitting analysis; the land surface temperature gradually decreases with the decrease of mining subsidence depth outside the impounded water area. [ABSTRACT FROM AUTHOR]