1. miR-214 stimulated by IL-17A regulates bone loss in patients with ankylosing spondylitis.
- Author
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Liu, Zizhong, Huang, Feng, Luo, Gui, Wang, Yiwen, Du, Ruikai, Sun, Weijia, Li, Jianwei, Yuan, Xinxin, Cao, Dengchao, Li, Yuheng, Liu, Caizhi, Liang, Shuai, Jin, Xiaoyan, Ling, Shukuan, Wang, Deqing, and Li, Yingxian
- Subjects
ANIMAL experimentation ,ANKYLOSING spondylitis ,BLOOD collection ,BONE marrow ,BONE resorption ,BONE growth ,CELL receptors ,CELLULAR signal transduction ,ENZYME-linked immunosorbent assay ,GENE expression ,INTERLEUKINS ,MEMBRANE proteins ,MICE ,OSTEOCLASTS ,POLYMERASE chain reaction ,RNA ,OSTEOBLASTS - Abstract
Objective Bone loss is common in AS, and miR-214 plays an important role in regulating bone formation. The aim of this study was to investigate the effect of miR-214, the production of which is stimulated by IL-17A, on bone loss in AS. Methods Peripheral blood was obtained from 32 patients with AS and 24 healthy controls. Levels of IL-17A, soluble RANK ligand (RANKL) and osteoprotegerin in serum were evaluated by ELISA, and the relative level of miR-214 in serum was detected by real-time quantitative PCR. In addition, we assessed the relationship between levels of miR-214, IL-17A and bone loss in primary murine osteoblasts and mouse bone marrow cells. Results The expression of RANKL and miR-214 in osteoblasts was increased following stimulation by IL-17A, and osteoblasts stimulated by IL-17A promoted the expression of miR-214 in osteoclasts and the activity of osteoclasts. We showed that osteoblast-derived miR-214 could be transferred to osteoclasts and could then regulate their activity. The levels of IL-17A and miR-214 were much higher in the serum of patients with AS than in that of healthy controls, and the relative level of miR-214 was positively correlated with the level of IL-17A in the serum and synovial fluid of the patients with AS, not healthy controls. The level of miR-214 in the serum of AS patients has potential diagnostic value. Conclusion The production of miR-214 in osteoblasts is stimulated by IL-17A. It is an important inhibitor of bone formation in AS, and the serum level of miR-214 might be of potential diagnostic value for AS. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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