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The abnormal concentration of adenosine 5-triphosphate (ATP) in cancer cells is closely related to the process of tumorigenesis and development. Therefore, rapid and accurate detection of ATP level in and out of cells is of great significance. Graphene oxide (GO) is widely used owing to its advantages of low toxicity, large specific surface area, easy functionalization, efficient and stable loading of DNA nanoprobes into cells. However, ssDNA bound to GO by physical adsorption is easily displaced by biomolecules in complex biological environment, leading to false positive signals. Based on this, a novel GO-DNA nanoprobe is proposed and applied to the detection of ATP and targeted delivery of the anticancer drug doxorubicin hydrochloride (DOX). Double stranded DNA (dsDNA) is formed by hybridization of aptamer of ATP with complementary strand, and then DOX is loaded by G-C base pair to form dsDNA-DOX. dsDNA-DOX is adsorbed on GO surface through polyA sequence extended by complementary strand to construct GO-dsDNADOX nanoprobes, which could greatly reduce false positive signal caused by the physical adsorption of biomolecules in complex environment. The specific binding of ATP to aptamer can lead to the release of DOX, and quantitative analysis of ATP is achieved according to its fluorescence “off-on”. DOX fluorescence intensity shows a good linear relationship with the ATP content within the range of 0. 08~8. 0 mmol/L, and the linear equation is IF=3. 0897c+129. 08. The detection limit (3σ/k) is 0. 059 mmol/L, and the method has good selectivity and anti-interference ability. Cytotoxicity and fluorescence imaging results show that the DOXloaded nanoprobes have significant drug release in MCF-7 cells and significantly induce apoptosis. This study establishes a label-free, simple and rapid method for ATP content detection and realizes targeted drug delivery by the high content of ATP in cancer cells, which reduces the toxic and side effects on normal cells and provides a new idea for cancer treatment. [ABSTRACT FROM AUTHOR]

To investigate the soil hydraulic characteristics across a wide range of suctions (0-10 MPa) of Benggang soil, the soil water holding capacities of different soil layers in Benggang was determined using the pressure film meter and the filter paper methods. Traditional and modified Van Genuchten-Mualem (VG) models were used to fit the soil moisture characteristics curves. The results showed that the water-holding curves determined by the pressure film meter and filter paper methods intersected and tended to overlap. This observation suggested combining the two measurement methods to obtain soil moisture characteristic curves over a wide suction range. Further, within the wide suction range, the water-holding capacity of the sandy soil layer was higher than that of the red and detritus soil layers in the low suction stage (h<0.1 MPa). However, in the high suction stage (h>0.1 MPa), the red soil layer had the highest water-holding capacity. Both the traditional and modified VG models were able to fit the soil moisture characteristics of each soil layer of the Benggang wall with a root mean square error of less than 0.021 5. The Nash efficiency and correlation coefficients were greater than 0.926 6 and 0.899 1, respectively. Noticeably, the modified VG model was more suitable for describing the soil moisture characteristic curves of the Benggang wall across a wide suction range. The residual values of the soil moisture characteristic curves for the red, sandy, and detritus layers were 378 kPa (corresponding to a water content of 18.82%), 173 kPa (corresponding to a water content of 18.95%), and 92 kPa (corresponding to a water content of 18.41%), respectively. Finally, the form of the segmental function (with the residual value as the inflection point) was more suitable for describing the unsaturated hydraulic conductivity over a wide suction range of Benggang soils than the conventional VG model. [ABSTRACT FROM AUTHOR]

Objective·To explore the effect of continuous positive urine ketone body in pregnant women with gestational diabetes mellitus (GDM) on the maternal and infant outcomes. Methods·A total of 168 GDM pregnant women who attended the Nutrition Clinic of the International Peace Maternal & Child Health Hospital, Shanghai Jiao Tong University School of Medicine from January 2021 to January 2022 and gave birth in the hospital were selected as the study subjects. According to the difference of urine ketone body test results in prenatal examination, they were divided into ketone body-positive group (56 cases, the pregnant women had ketone body test positive for three times or more after the diagnosis of GDM) and ketone body-negative group (112 cases, the ketone body test was continuously negative after the diagnosis of GDM). The general information included clinical data and laboratory indicators of pregnant women and clinical outcomes of pregnant women and their newborns were collected and compared. Results·There were no statistically significant differences in clinical data and laboratory indicators of pregnant women between the two groups, among which the body mass index before pregnancy, weight change before diagnosis of GDM, family history of diabetes mellitus, history of GDM, total cholesterol and triacylglycerol levels in the early pregnancy, fasting blood glucose and insulin levels in the middle pregnancy had no significant correlation with the production of urine ketone body. The results of clinical outcome indicators of pregnant women showed that there were statistically significant differences in weight change (P= 0.000; RR=6.000, 95% CI 1.251 ‒ 28.777) and rate of weight gain (P=0.000; RR=1.829, 95% CI 1.132 ‒ 2.953) after diagnosis of GDM between the two groups. The results of neonatal clinical outcome indicators showed that there was statistically significant difference in the neonatal hospitalization rate (P=0.023; RR=2.167, 95% CI 1.059 ‒ 4.434) between the two groups; among them, there were 4 adverse events in the ketone body positive group and no such adverse events in the ketone body negative group. Conclusion·The continuous positive urine ketone body of pregnant women with GDM may increase the risk of insufficient weight gain in pregnant women and neonatal hospitalization. It is recommended to timely detect and intervene in clinical practice. [ABSTRACT FROM AUTHOR]

Objective To explore the current situation of disease prevention and control ability and training needs of grassroots public health personnel in Luzhou, to provide a reference for improving the disease prevention and control ability of grassroots public health personnel in Luzhou, Methods A questionnaire survey was conducted on 1 075 people engaged in disease control work in township health centers, village clinics, and community health service centers (stations) in Luzhou City to analyze their disease prevention and control ability and influencing factors. Results The average self 一 evaluation score of basic disease prevention and control ability skills of primary public health personnel in Luzhou City was 57. 93 ± 27. 30 points, with a score rate of 46. 72%. Among them, r prevention and control of chronic non - communicable diseasesir had the highest score rate of 56. 94%, and, r prevention and control of endemic and parasitic diseases" had the lowest score rate of 37. 75%. Among the survey respondents, 92.37% had participated in basic knowledge or skills training in disease control, and the proportion of those who chose, r on - site guidance1 r as the desired form of training was the highest, at 80. 74%. The results of multiple linear regression analysis showed that the factors affecting the ability of primary public health personnel in disease prevention and control were mainly female(£ 二-3. 425, P = 0. 001), outside the establishment(£ 二 3. 322, P = 0. 001), not having participated in training in basic knowledge or skills of disease control(£ 二 -6. 119, P < 0.001), professional background (non 一 medical t - -2. 083, P = 0. 038) and professional background (non 一 medical £ 二 一 0. 083, P = 0. 038). Conclusion The disease prevention and control ability of grassroots public health personnel in Luzhou City is relatively weak, with gender, professional background, establishment, and whether they have participated in training being the main influencing factors, requiring targeted training in relevant knowledge and skills. [ABSTRACT FROM AUTHOR]

Using our team's recent research achievement “the cervical ultrasound scanning route map” as a teaching tool, to evaluate its impact on the learning effectiveness and confidence of beginners in the process of learning cervical spinal ultrasonography. Methods This study is a before and after self-control study. After recruitment of 40 cervical ultrasound beginners, they had completed questionnaire 1. The questionnaire was designed with three self-assessment questions on theoretical knowledge of cervical spinal ultrasound, three self-assessment questions on practical skills of cervical spinal ultrasound and one question on willingness to use cervical spinal ultrasound in the future. After traditional teaching without involving the route map, beginners completed questionnaire 2 with the same content. After a 20 min break, teacher introduced and explained the route map to the beginners, then the beginners completed questionnaire 3. Questions related to satisfaction with this learning experience had been added to questionnaire 3. The answers to all questions were scored on a scale of 1-10. In this study, the main outcome was the comprehensive learning score, calculated as(sum score of theoretical knowledge + sum score of practical skills + score of willingness to use cervical ultrasound in the future)/7. Results Beginners'comprehensive learning score at the three time points were 2.9±1.3, 4.8±1.8, 5.7±1.8,F(2,22)=52.11,P＜0.001,partial Eta squared=0.83. After introducing the route map, their comprehensive learning score increased 1.0(95% CI:0.46-1.49)(P＜0.001) compared to scores after traditional training. Conclusions The route map may significantly improve learning effectiveness and confidence of beginners in the field of cervical spinal ultrasonography and can be used as a routine training tool in the teaching of cervical spinal ultrasonography. [ABSTRACT FROM AUTHOR]

Objective To explore the kinase activity of novel receptor interacting protein kinase 3 (RIPK3) mutants. Methods 瞬 The four amino acids (Q84WDF87) of RIPK3 were mutated respectively and these mutants were co-transfected with mixed lineage kinase domain like pseudokiiiase (MLKL) into HEK293T cells. The auto-phosphoiylation of these mutants at S232 and phosphorylation of MLKL at S345 were detected by Western blotting. The interaction between RIPK3 and MLKL was tested by co-immimoprecipitatiotL The oligomerization of MLKL was detected by non-reducing gel. Results The kinase activities of REPK3AQ84, RIPK3AW85 and RIPK3AD86 were effectively decreased. Nevertheless, the kinase activities of RIPK3Q84A/RIPK3Q84E, RIPK3W85Y and RIPK3D86A/RIPK3D86Y did not change markedly. The auto-phosphorylatioii of RIPK3W85A at S232 was decreased without affecting phosphorylatioii and oligomerization of MLKL. Conclusion • The amino acid site Q84, W85 or D86 plays a critical role in RIPK3 kinase activity. The kinase activity ofRIPK3W85Ais decreased, but it does not affect MLKL. [ABSTRACT FROM AUTHOR]

Objective To construct a prognostic prediction model for chronic postsurgical pain after video-assisted thoracoscopic surgery and develop a risk evaluation tool. Methods As a single-center prospective study, the study enrolled 1 132 patients received video-assisted thoracoscopic surgery between November 2020 and May 2021 at Peking Union Medical College Hospital. Numeric rating scale was used to assess the severity of acute pain when they were discharged and chronic pain at 6 months after surgery. Patients were divided into two groups based on the presence or absence of chronic postsurgical pain. Multivariate Logistic regression was conducted to analyze the risk factors which were used to construct prediction model and draw nomogram for chronic postsurgical pain after video-assisted thoracoscopic surgery. Results 460 patients (40.6%) were reported chronic pain at 6 months after surgery. More women and more patients with coronary heart disease belonged to the group of chronic postsurgical pain. Patients reported higher scores of numeric rating scale when they were discharged and inclined to keep taking painkiller after discharge. However, there were fewer patients with incisions fewer than 3 or using nitrous oxide during surgery in the group of chronic postsurgical pain (P＜0.05). The outcome of multivariate Logistic regression showed that the history of coronary heart disease, not using nitrous oxide in surgery, incisions more than or equal to 3, and the score of numeric rating scale on discharge were independent risk factors for chronic postsurgical pain (P＜0.05). The area under the receiver operator characteristic curve of the prediction model was 0.734. Conclusions The prediction model is conducive to clinical management for chronic postsurgical pain after video-assisted thoracoscopic surgery. [ABSTRACT FROM AUTHOR]

Objective To explore the risk factors of adverse discharge outcomes in patients with acute ischemic stroke (AIS), and the effects of post-stroke infection on discharge outcomes. Methods AIS patients who were hospitalized in the Department of Neurology, Beijing Tiantan Hospital, Capital Medical University from June 2019 to June 2022 were retrospectively included in this study. The patients were divided into good outcome group (mRS score<3) and poor outcome group (mRS score >3 or death during hospitalization) according to discharge outcome. The clinical characteristics and post-stroke infection(pulmonary infection, urinary tract infection and central nervous system infection) were compared between the two groups. Multivariate logistic regression was used to analyze the risk factors for adverse discharge outcomes in AIS patients. Results A total of 1024 patients with AIS were included in this study, with a mean age of (60.3±12.4) years old and 788 males (77.0%). There were 761 (74.3%) patients in the good outcome group and 263(25.7%) patients in the poor outcome group. 85 (8.3%) patients developed infection after stroke, of which 69 (6.7%) were pulmonary infections, 16 (1.6%) were urinary tract infections, and 3 (0.3%) were central nervous system infections. Multivariate analysis showed that pulmonary infection (OR 2.522, 95%CI 1.318-4.828, P=0.005), diabetes (OR 1.486, 95%CI 1.048-2.106, P=0.026), increased NIHSS score on admission (OR 1.286, 95%CI 1.233-1.342, P<0.001) and increased white blood cell count (OR 1.094, 95%CI 1.014-1.180, P=0.020) were risk factors for poor discharge outcomes in AIS patients. Conclusions Pulmonary infection may significantly increase the risk of poor discharge outcomes in patients with AIS. [ABSTRACT FROM AUTHOR]

This study aimed to clarify the spatial differentiation of soil geotechnical characteristics in Benggang area and reveal the quantitative relationship betweensoilphysicochemical properties and shear strength. The differences of soil physicochemical properties and shear strength at different depths vertically (red soil, sandy soil and detritus layers) and horizontally (surface and inner soils) were analyzed in a typical Benggang area of Anxi County, Fujian Province, China. The contents of free and amorphous iron and aluminum oxides in surface soil exceeded those in the inner soil for the three soil layers. There were no obvious differences in organic matter, pH value, particle composition, and shear strength parameters between the surface and the inner soils. Significant differences were found in soil properties among the different soil layers of gully wall. The contents of free and amorphous iron and aluminum oxides, and clay particles of the red soil layer were the greatest, and the cohesion of red soil layer was the largest. The smallest values were obtained for the detritus layer. Correlation analysis revealed significant positive correlation between free and amorphous iron and aluminum oxides, organic matter, medium silt and smaller fine particles, and soil cohesion. Significant negative correlation was found between free manganese oxide, coarse silt and larger coarse particles, and soil cohesion. The internal friction angle positively correlated with free iron oxide. Stepwise regression analysis showed that amorphous aluminum, free iron oxide, fine clay particles, and fine silt particles were the main soil factors affecting the soil shear strength of the gully wall (r=0.96, P<0.01, RMSE=10.44). [ABSTRACT FROM AUTHOR]