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By analyzing the of genetic testing data of patients with renal polycystic kidney disease and their relatives, this study aims to identify unreported novel gene mutation sites associated with autosomal dominant polycystic kidney disease (ADPKD). Structural prediction software was employed to investigate protein structural changes before and after mutations, explore genotype-phenotype correlations, and enrich the ADPKD gene database. In this single-center retrospective study, patients with multiple renal cysts diagnosed from January 2019 to February 2023 at the Zhong Da Hospital Southeast University were included. Genetic and clinical data of patients and their families were collected. Unreported novel gene mutation sites associated with ADPKD were identified. The AlphaFold v2.3.1 software was used to predict protein structures. Changes in protein structure before and after mutations were compared to explore genotype-phenotype correlations and enrich the ADPKD gene database. Twelve mutated genes associated with renal cysts were detected in 52 families. Nineteen novel gene mutation sites associated with ADPKD were identified, including 17 mutations in the PKD1 gene (one splicing mutation, seven frameshift mutations, four nonsense mutations, one whole-codon insertion, and four missense mutations); one ALG9 missense mutation; and one chromosomal structural variation. Truncating mutations in the PKD1 gene were correlated with a more severe clinical phenotype, while non-truncating mutations were associated with greater clinical heterogeneity. Numerous novel gene mutation sites associated with ADPKD remain unreported. Therefore, it is essential to analyze the pathogenicity of these novel mutation sites, establish genotype-phenotype correlations, and enrich the ADPKD gene database.

Objective: To study the oxidative damage of PM2.5 in human embryonic stem cells (EBf-H9 cells), and to provide a theoretical basis for revealing the adverse health effects of PM2.5 and the potential mechanisms, and also to provide a new alternative cell model for PM2.5 risk assessment., Methods: EBf-H9 cells were cultured with 0.00 (the constrast group) 3.91, 7.81, 15.63, 31.25, 62.50, 125.00 μg/cm(2) of PM2.5. CCK-8 kit was used to determine the survival rate of cells exposed to PM2.5 for 6 h. DCFH-DA probe was used to detect the total ROS content of cells exposed to PM2.5 for 1 h. The activities of total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-Px), and the content of lipid peroxides such as malondialdehyde (MDA) in cells exposed to PM2.5 for 6 h were detected by using the commercial kits. ANOVA model analyzed the statistical significance from the different concentration group., Results: The cytotoxicity results showed that the cell survival rate was decreased gradually with the increase of the concentrations of PM2.5, and the half inhibitory concentration (IC50) was 83.01 μg/cm(2). When the exposure concentration was 3.91, 7.81, 15.63, 31.25, 62.5 μg/cm(2), after exposure of PM2.5 1 h, the ROS florescence was 27.12±0.21, 54.03±0.50, 60.93±0.08, 61.36±1.00, 68.21±0.93, 78.27±1.26 (compared to control group 27.12±0.21, all P level<0.01). After exposure of PM2.5 6 h, the activities of T-SOD was (9.78±0.28), (8.59±0.22), (8.90±0.33), (7.46±0.71), (4.21±0.17) U/mg protein (F=98.881, compared to control group (11.77±0.63) U/mg protein, all P level<0.01). The activities of GSH-Px was (181.59±3.65), (153.33±1.69), (168.74±2.22), (81.56±0.56), (48.62±2.13) U/mg protein (compared to control group (273.90±6.50) mU/mg protein, all P level<0.01). And the content of MDA was (0.38±0.03), (0.43±0.09), (0.47±0.09), (0.65±0.10), (0.70±0.12) nmol/mg protein (compared to control group (0.27±0.02) nmol/mg protein, all P level<0.05)., Conclusion: PM2.5 exposure can decrease EBf-H9 cells viability, and improve the levels of lipid peroxidation. It may be due to induce EBf-H9 cells to increase the production of ROS and to make the cells appear oxidative stress, which lead to oxidative damage to cells. The present study reveals the mode of action of PM2.5 in terms of oxidative damage to EBf-H9 cells. It is also indicated that the cells may be a new alternative cell model for PM2.5 risk assessment.

Objective: To assess the oxidative damage after exposure to fine particulate matter (PM2.5) in human umbilical vein endothelial cells (HUVECs) and to explore the influence of the Nrf2 pathway., Methods: HUVECS were exposed to different concentrations of PM2.5 as follows: 0.000 (control), 0.004, 0.039, 0.391, 1.950, 3.910, 7.810, 15.600, 31.250, 62.500, 125.000 and 250.000 μg/cm(2). After 24 h, cell viability was measured by the CCK-8 method. In a separate experiment, HUVECs were exposed to 0 (control), 1.95, 3.91, 7.81, 15.63 or 31.25 μg/cm(2) of PM2.5. The level of cellular reactive oxygen species (ROS) was detected with an H2-DCFDA fluorescence probe after 1h and 3 h exposure. After 24 h exposure, the activity of superoxide dismutase (SOD) and glutathione peroxidase (GPx), and malondialdehyde (MDA) content were detected by colorimetry. Western blot was used to estimate the expression levels of Nrf2 and NQO1 in total protein., Results: HUVEC viability was reduced in a concentration-dependent manner by PM2.5. Compared with controls (100% viability), the viability of the 250 μg/cm(2) group was (38.18±6.68)% (P<0.05). Substantial accumulation of ROS occurred in HUVEC after 1 h and 3 h exposure to PM2.5. After 24 h exposure to 0, 1.95, 3.91, 7.81, 15.63 and 31.25 μg/cm(2) of PM2.5, SOD activity decreased concentration-dependently to (26.25±1.76), (24.99±1.81), (24.25±0.49), (22.07±1.13), (21.03±0.43) and (19.37±0.84) U/mg protein, respectively, (F=13.95, P<0.001). GPx activity decreased in a concentration-dependent manner to (25.63±1.33), (24.40±2.20), (22.85±2.46), (20.98±1.95), (20.17±1.86) and (18.69±3.11) mU/mg protein, respectively (F=4.26, P=0.019), whereas MDA increased concentration-dependently to (1.11±0.07), (1.12±0.07), (1.17±0.05), (1.49±0.01), (1.95±0.08) and (2.37±0.08) nmol/mg protein, respectively, (F=186.37, P<0.001). Compared with the control Nrf2, NQO1 protein levels (1.00±0.27, 1.00±0.33), 15.63 μg/cm(2) group (2.38±0.44, 1.78±0.20) were enhanced (P<0.05)., Conclusion: These results demonstrate that PM2.5 can lead to oxidative damage to HUVEC in a concentration-dependent manner. Protein levels of Nrf2 and NQO1 were enhanced at high concentrations of PM2.5, and this mechanism may be related to increases in cellular ROS induced by PM2.5.

The etiology of rabies is caused by the bite of a mad dog infected by non-seasonal pathogen with intoxicated viscerae, and its pathogenesis being heat and blood stasis inside the body. The evolution of the knowledge of rabies experienced a process of from the recognition of biting of a mad dog in the Sui-Tang Dynasties to the systematic manifestations of the central nervous system in the Ming-Qing Dynasties. The recognition in its diagnosis, therapeutic effects, and prognosis is in line with the diagnostic knowledge carried in modern Textbook of Infectious Diseases. It pays attention to the cleaning of the trauma, experiencing a process from sucking by the mouth, washing with water to washing with raw glycyrrhiza and urine, while the development of therapy evolved from a single-recipe-special-drug level to the patent prescription level.

Objective: To study the effect of deoxynivalenol (DON), a mycotoxin produced by Fusarium graminearum, on action potentials of cultured cardiomyocytes and the possible protective effects of sodium selenite., Methods: Ventricular myocytes from neonatal Wistar rats were cultured, and the transmembrane action potentials were recorded with glass microelectrodes before and after addition of DON at different concentrations. The cultured cardiomyocytes were pretreated with 0.5 mg/L selenium (as sodium selenite) to observe the protective effects of selenium against the effects of DON., Results: DON at concentrations of 50, 100 and 200 mg/L decreased the action potential parameters including action potential amplitude (APA), overshoot (OS), threshold potential (TP), maximum rate of depolarization (Vmax) and action potential discharging frequency (APF), and prolonged the action potential duration of 10%, 50% and 90% repolarization (APD(10), APD(50) and APD(90)). Some of the parameters, such as APA, Vmax, APD and APF, changed in a concentration-dependent manner. The cultured cardiomyocytes pretreated with 0.5 mg/L of selenium for about 16 h presented only slight changes in action potential parameters induced by 200 mg/L DON., Conclusions: DON inhibit the membrane action potentials of cardiomyocytes, suggesting DON may interfere with the transmembrane movement of Ca(2+) and K(+), and sodium selenite may decrease the toxic effect of DON on cultured cardiomyocytes.