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In order to explore reasonable artificial cultivation pattern of Thesium chinense, the biological characteristics and nutrients change in the process of winter dormancy of T. chinense was studied. The phenological period of T. chinense was observed by using fixed-point notation and the starch grains changes were determined dynamically by PAS-vanadium iron hematoxylin staixjing method. Soluble sugar and starch content were measured by anthrone-sulfuric acid method and amylase activity was determined by DN'S method. The results showed that the normal life cycle of T. chinense was two years. T. chinense was growing by seed in the first year, but growing by the root neck bud in the second year. During the process of dormancy, starch and soluble sugar could mutual transformation in different periods. T. chinense had sufficient carbohydrate to maintain growth and also a lot of small molecules to improve their ability to fight against adversity.

Objective: To study the quality of medicinal material of Thesium chinense., Method: The active ingredients of total flavonoids, kaempferol, mannitol, polysaccharides were selected as the evaluative indicators to comprehensively evaluate the quality of medicinal material of T. chinense., Result: The total flavone 3.38%, kaempferol 0.984 7 mg x g(-1), mannitol 6.12%, polysaccharides 14.08% and water soluble extract 30.28% were measured in the sample of T. chinense. There was a certain correlation among these selected active ingredients. The compositions of most biological activities component in T. chinense of different populations were similar, but the contents were different significantly. The content of activities component in T. chinense showed a general tendency of declining during growth period and with the increase time of storage., Conclusion: Flavone, kaempferol, mannitol, polysaccharides should be selected as the indicators to evaluate the quality of medicinal material of T. chinense.

Objective: To determine the concentration of mineral elements and analyze the principal components in Thesium chinense., Method: Mineral elements were determined by ICP-DES. Principal components analysis (PCA) was used toanalyze and evaluate the characteristic elements., Result: T. chinense contained more than 17 mineral elements, Fe, Zn, Cu, Mn, Cr, Co, Ni, Sr, B, Ca, P, K, Na, Mg, Mg, Mn, Zn and Cu were abundance in T. chinense. The concentration of Mn and Zn were about 100 microg x g(-1), Mg was from 1 898.0 to 3 137.73 micro x g(-1), Fe > 500 microg x g(-1). PCA concluded that four factors (F1, F2, F3, F4 ) could be used to evaluate the quality of T. chinense. The function is following: F = 0.444 77F1 + 0.237 71F2 + 0.167 28F3 + 0.150 24F4. The scores of different populations of T. chinens were from 0.627 to 0.189. Zn,Cu,Mg,P, K, Al, Fe, Cr, Na, Co, Pb, Mn, B and Ca were the characteristic elements of T. chinense., Conclusion: T. chinense contain more than 17 mineral elements. Zn, Cu, Mg, P, K, Al, Fe, Cr, Na, Co, Pb, Mn, B and Ca were the characteristic elements of T. chinense.

Objective: To investigate the activity and the location of the endogenesis inhibitory substances in seed of of Thesium chinense., Method: The rough extracts from different concentrations, places and extracting times of T. chinense seeds were prepared and determined by its effect on wheat and Brassica sp seed., Result and Conclusion: The extract dramatically inhibited the germentation and the growth of wheat and Brassica sp seed and the inhibitory activity was positively related to the concentration of the extracts. When the concentration reached 0. 32 g mL(-1), wheat seed did not germentate. With increasing the extracting times, the inhibitory activity first decreased, then increased. The capsule and kernel both had inhibitory substances and the latter was stronger than the former. Highly active inhibitory substances were found first time in the seed of T. chinense.

Objective: To establish a determination method of astragulin in Thesium chinese., Method: RP-HPLC analytical method was established using a Polaris C18 column and acetonitrile-water (23:77) as the mobile phase, with a flow rate of 10 mL x min(-1), detected at 346 nm. The method of sample is refluxing exation by 50% alcohol for 2 times., Result: The content of astragulin was from 0. 120% to 0. 155%, in different groups of T. chinese collected from the same location., Conclusion: The method was validated to show convenient and reliable.

Objective: To evaluate the wind-damage effects on quality of heartwood of Lignum Santali Albi., Method: GC-MS, TLC and pharmacodynamic test., Results: The content of volatile oil from heartwood of Wind-damaged Lignum Santali Albi is 1.42%; the content of various components in the oil and the chromatography of different extracts are similar to those of reference drug and 25 years old trees., Conclusion: Wind-damage should accelerate the formation of heartwood of Lignum Santali Albi without influence on its quality.