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Background: The factors associated with infection in oral candida are countless. This infection is mainly caused by bad prosthetic cleanliness. Objective: The goal of current research study was to assess that how oral candida growth is influenced by wearing false teeth all over night and gender. Patients and Methods: The present research was organized at Sir Ganga Ram Hospital, Lahore (October 2017 to August 2019). Total patients enrolled for this research were forty. The males and females were in equal number i.e. 20. These patients were between fifty to sixty-five years of age and complete denture wearer for first time. Those patients were not selected for the study who were found with smoking habit, diabetes and with the record of treatment with radiotherapy and chemotherapy in the previous six months. For the assemblage of sample, the method used was oral rinse on some time in a day. In this method, each patient was given ten milliliter of sterile saline in a sterile container that was disposable. They were asked to rinse for the time period of sixty seconds. Quantitative and qualitative growth of candida was examined and Gram stain, microscopy and colony count were measured after sending the sample to Microbiology section of pathology department of the hospital. Results: It is concluded by the results that candida growth is increased by overnight denture wearing. It was also observed that after one month of wearing complete denture, the dissimilarity in candida growth in females was valuable. Conclusion: It is concluded that dentures should be removed during night, as in case of eyeglasses. In this way, denture stomatitis will be avoided. Moreover, information should be provided about oral cleanliness to common people, especially to females. Keywords: Denture, Candida and Sterile Container.