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Background: Justicia carnea has overwhelming evidence demonstrating its medicinal and nutritional benefits, some of its phytochemicals are antinutritional and can have negative consequences including impairment of the male reproductive functions when ingested. The adverse effects on male reproductive function by ethanol leaf extract of Justicia carnea has been reported including alteration of the antioxidant system of the testes, reduction in the weight of testes, reduced sperm count/motility and testosterone levels as well as distortion of the histoarchitecture of the testis in male wistar albino rats. Most agents that alter male fertility do so by altering the antioxidant system of the testes.  Aims and Objectives: The aim of this study is to determine the varieties of phytochemicals present in both boiled aqueous and ethanol extracts of the leaves of Justicia carnea, the impact of administration of these extracts on the testicular antioxidant system status (superoxide dismutase - SOD, catalase - CAT, malonaldehyde - MDA and reduced glutathione - GSH) and testicular/epididymal histology of male Wistar Albino rats.Methods: The GC-MS of both boiled aqueous extracts (AJC) and ethanol extracts (EJC) of the leaves of Justicia carnea were carried out to determine the phytochemical components of both extracts which were administered to the experimental male Wistar Albino rats. Forty-Two Albino Wistar rats (12-14 weeks of age) in seven groups (I-VII) with average weight of 180-200g were used for the study. The control was fed normal grower feed and water only. The test groups were in addition given 200, 400 and 600 mg/kg BW of either boiled aqueous or ethanolic-leaf extracts of Justicia carnea for a period of 21 days. The first batch of the experimental animals (drawn from each group) were sacrificed on day 21 and the second batch were fed normal grower feed and water only for further 52 days after discontinuation of the extracts before sacrificing them. Testicular tissue redox status was determined by assaying for superoxide dismutase (SOD) using Sun and Zigma method, catalase by Aebi method, Malondialdehyde (MDA) by Buege and Aust method, reduced glutathione (GSH) by Sedlak and Lindsay method. The histology of the testis/epididymis was also carried out. Statistical Package for the Social Sciences (SPSS) version 25 was used for analysis of the results.Results: The GC-MS results show that the ethanol extract has six different known male antifertility compounds while the boiled aqueous extracts have two. There was no significant change (P < 0.05) in the level of testicular SOD after 21 days of administration of the extracts except for the group that received 400mg/kg BW of AJC which had a significantly higher level that was reversed 52 days after discontinuation. However, at 52 days after discontinuation, the group that received 400mg/kg BW of EJC had significantly higher SOD (P < 0.05) compared to the rest. Testicular catalase (CAT) was reduced but not significantly (P < 0.05) in all the experimental groups experimental groups at 21 days after administration of both extracts but exhibited variable changes at 52 days after withdrawal of administration of the extracts. While there was a non-significant reduction (P < 0.05) in the levels of CAT amongst most groups, there was a non- significant increase (P < 0.05) in the group that received 400mg/kg BW of AJC and a significant increase (P < 0.05) in the group that received 400mg/kg BW of EJC. The administration of 400mg/kg BW, 600mg/kg BW of EJC and 600mg/kg BW of AJC caused a significant decrease (P < 0.05) in the levels of reduced glutathione (GSH), while 400mg/kg BW of AJC caused a non- significant reduction (P < 0.05) after 21 days of administration. At lower doses, 200mg/kg BW of AJC caused a non-significant increase (P < 0.05) while 200mg/kg BW of EJC caused a significant increase (P < 0.05) in the levels of GSH after 21 days of administration. At 52 days after discontinuation, the group that received 200mg/kg BW of EJC still had a sustained significantly higher (P < 0.05) level of GSH, while the group that received 200mg/kg BW AJC still had no significant change (P < 0.05) in the level of GSH. However, the group that received 400mg/kg BW of AJC and 600mg/kg BW EJC still showed a significantly lower (P < 0.05) GSH while those that received 400mg/kg BW of EJC and 600mg/kg BW of AJC had a non-significant decrease (P < 0.05) in the GSH activity 52 days after withdrawal. Many of the treatment groups show significantly higher level (P < 0.05) of testicular malondialdehyde (MDA) after 21 days of treatment, except the groups that received 200mg/kg BW of AJC and 600mg/kg BW of EJC which showed non-significantly lower (P < 0.05) levels and unchanged levels respectively. The, group that received 400mg/kg BW of EJC had the least MDA levels at 21 days after administration which is significantly lower (P < 0.05) than the control. Most of the changes in the levels of MDA were in reversal at 52 days after discontinuation of the extracts as the 200mg/kg BW, 400mg/kg BW of EJC and 400mg/kg of AJC groups then had non-significantly elevated (P < 0.05) MDA levels. While the group that received 200mg/kg BW of AJC had significantly lower levels (P < 0.05) of MDA, the group that received 600mg/kg BW of EJC and 600mg/kg BW of AJC had non-significantly lower (P < 0.05) MDA levels. There was no obvious distortion of testicular histology or histoarchitecture in any of the experimental groups as the epididymis displayed normal ducts, while the testisshowed seminiferous tubules with layers of germ cells at various stages of maturation.Conclusion: There are numerous male antifertility phytochemicals in both extracts of Justicia carnea. This study shows that boiled aqueous and ethanol extracts of Justicia carnea leaves can have a negative impact on the testicular antioxidant system and by extension the male fertility potentials but no recognizable effect on the histoarchitecture of the testes at the doses used in this study.