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This protocol describes the tissue preparation processes for the CODEX® (now PhenoCycler™) system by Akoya Biosciences. For the comprehensive multiplexed imaging workflow currently in use at the Vanderbilt Diabetes Research Center, please see CODEX® Multiplexed Imaging | Modality overview.

This protocol describes the workflow for collecting human pancreas samples for multimodal downstream assays, in use by the Vanderbilt University HuBMAP Tissue Mapping Center.

This protocol for H&E staining can be applied to either fixed or unfixed frozen cryosections.

This protocol describes the imaging workflow currently in use by the Vanderbilt Diabetes Research Center Islet & Pancreas Analysis (IPA) Core and Powers/Brissova Research Group for CO-Detection by indEXing (CODEX®, now PhenoCycler™; Akoya Biosciences). See also CODEX® Multiplexed Imaging | Modality overview.

This protocol describes the staining and pre-imaging preparation currently in use by the Vanderbilt Diabetes Research Center Islet & Pancreas Analysis (IPA) Core and Powers/Brissova Research Group prior to performing multiplexed imaging of the human pancreas using the CO-Detection by indEXing (CODEX®) platform (now PhenoCycler™; Akoya Biosciences). See also CODEX® Multiplexed Imaging | Modality overview.

This protocol describes the antibody conjugation and validation processes for the CODEX® (now PhenoCycler™) system by Akoya Biosciences. For the comprehensive multiplexed imaging workflow currently in use at the Vanderbilt Diabetes Research Center, please see CODEX® Multiplexed Imaging | Modality overview.

This is an overview of all protocols currently in use by the Vanderbilt Diabetes Research Center Islet & Pancreas Analysis (IPA) Core and Powers/Brissova Research Group for performing multiplexed imaging of the human pancreas using the CO-Detection by indEXing (CODEX®) platform (now PhenoCycler™; Akoya Biosciences). It includes links to each of the individual protocols that make up the workflow for various applications and projects.

This protocol for H&E staining can be applied to either fixed or unfixed frozen cryosections.

Procuring and preparing human pancreas for paraffin-embedding, fixed cryosections, flash frozen sections, and RNA isolation. This protocol was developed in collaboration with Dr. Rita Bottino of Imagine Pharma and Allegheny Health Network and is based on protocols developed by Dr. Marcela Brissova of Vanderbilt University.