1. Genetic Diversity of Lotus (Nelumbo nucifera Gaertn.) Cultivars Revealed by ISSR Analysis.
- Author
-
Tianyou HE, Yinquan QU, Shaoyan SHEN, Xiaoyun MO, Lingyan CHEN, Yingjia LIU, Jundong RONG, and Yushan ZHENG
- Subjects
- *
EAST Indian lotus , *PLANT genetics , *CULTIVARS , *DNA primers , *PLEOMORPHIC fungi - Abstract
[Objectives] This study was conducted to investigate the genetic diversity of 20 lotus (Nelumbo nucifera Gaertn) samples. [Methods] On this optimal ISSR amplification system, 16 primers were screened with good polymorphism. and the DNA was used to amplify the 20 plant samples. [Results] The 16 primers produced 225 loci, of which 170 were polymorphic, and the polymorphic loci percentage was up to 75. 56% . The genetic similarity coefficients between the 20 varieties ranged from 0.5778 to 0.9511. which were calculated by POPGENE32. The 20 varieties by UPMGA analysis could be clustered into 2 groups, the first of which included Baiyangdian red lotus and Donggua lotus, and other varieties was included in the second group. [Conclusions] ISSK molecular markers could be effectively used in genetic diversity and fingerprint analysis for differenly lotus varieties. [ABSTRACT FROM AUTHOR]
- Published
- 2017