Search

In this study, an embryogenic callus induction and proliferation system for Hippeastrum vittatum was established, with the tender bulbs as expiants. And then the sensitivity of the expiants and calli to kanamycin and hygromycin was evaluated. The results suggested that the embryogenic calli were induced from tender bulbs and cultured in Murashige and Skoog (MS) medium supplemented with 0.5 mg/L N-phenyl-N'-l, 2,3-thiadiazol-5-ylurea (TDZ), 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D), and 30 g/L sucrose (pH5.8) in the dark at 25 ± 1°C. Further study of the influence of kanamycin and hygromycin on callus induction and multiplication showed that, the lethal doses of kanamycin and hygromycin to bulb expiants were 100 and 30 mg/L, respectively. All expiants of H. vittatum died on the medium supplemented with 100 mg/L kanamycin or 30 mg/L hygromycin at the induction stage, and callus proliferation was completely inhibited by 100 mg/L kanamycin or 25 mg/L hygromycin, and all the calli died at last. These results will provide important reference for further studies of transgenic H. vittatum. [ABSTRACT FROM AUTHOR]

[Objective] This study aimed to investigate the tissue culture and propagation technology in Tagetes patula L. [Method] By using tissue culture technology, different mass fractions of 6-BA and NAA were added to MS medium to compare the effect of different culture medium on the rapid propagation of T. patula L. [Result] Shoot tips or stem segments of T. patula L. were used as explants for tissue culture with an appropriate sterilization time of 8 min; differentiation effect of shoot tips was better than that of stem segments; callus generation rate was high with the high content of growth regulators; MS medium containing 0.1 mg/L NAA and 1.5 mg/L 6-BA was used for subculture proliferation with a subculture period of 4 weeks; rooting rate of plantlet was the maximum (97% ) in 1/2MS medium containing 0.2 mg/L NAA, and the root system was relatively developed. [ Conclusion] This study provided technical support for the industrialized seedling breeding of T. patula L. [ABSTRACT FROM AUTHOR]