1. Promoter islands as a platform for interaction with nucleoid proteins and transcription factors.
- Author
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Purtov, Yuri A., Glazunova, Olga A., Antipov, Sergey S., Pokusaeva, Viktoria O., Fesenko, Eugeny E., Preobrazhenskaya, Elena V., Shavkunov, Konstantin S., Tutukina, Maria N., Lukyanov, Viktor I., and Ozoline, Olga N.
- Subjects
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PROMOTERS (Genetics) , *NUCLEOIDS , *TRANSCRIPTION factors , *BACTERIAL genetics , *ESCHERICHIA coli , *OLIGONUCLEOTIDES , *MESSENGER RNA , *ESCHERICHIA coli proteins - Abstract
Seventy-eight promoter islands with an extraordinarily high density of potential promoters have been recently found in the genome of Escherichia coli. It has been shown that RNA polymerase binds internal promoters of these islands and produces short oligonucleotides, while the synthesis of normal mRNAs is suppressed. This quenching may be biologically relevant, as most islands are associated with foreign genes, which expression may deplete cellular resources. However, a molecular mechanism of silencing with the participation of these promoter-rich regions remains obscure. It has been demonstrated that all islands interact with histone-like protein H-NS - a specific sentinel of foreign genes. In this study, we demonstrated the inhibitory effect of H-NS using Δ hns mutant of Escherichia coli and showed that deletion of dps, encoding another protein of bacterial nucleoid, tended to decrease rather than increase the amount of island-specific transcripts. This observation precluded consideration of promoter islands as sites for targeted heterochromatization only and a computer search for the binding sites of 53 transcription factors (TFs) revealed six proteins, which may specifically regulate their transcriptional output. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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