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Start Over Author "Chuang, Nie" Publisher wolters kluwer
3 results on '"Chuang, Nie"'

2. Correlation of In Vivo and In Vitro Methods in Measuring Choroidal Vascularization Volumes Using a Subretinal Injection Induced Choroidal Neovascularization Model

Author

Chuang Nie, Mao-Nian Zhang, Hong-Wei Zhao, Thomas D Olsen, Kyle Jackman, Lian-Na Hu, Wen-Ping Ma, Xiao-Fei Chen, Juan Wang, Ying Zhang, Tie-Shan Gao, Hiro Uehara, Balamurali K Ambati, and Ling Luo

Subjects
Choroidal Flatmount, Choroidal Neovascularization, Correlation, Spectral-domain Optical Coherence Tomography, Medicine
Abstract

Background: In vivo quantification of choroidal neovascularization (CNV) based on noninvasive optical coherence tomography (OCT) examination and in vitro choroidal flatmount immunohistochemistry stained of CNV currently were used to evaluate the process and severity of age-related macular degeneration (AMD) both in human and animal studies. This study aimed to investigate the correlation between these two methods in murine CNV models induced by subretinal injection. Methods: CNV was developed in 20 C57BL6/j mice by subretinal injection of adeno-associated viral delivery of a short hairpin RNA targeting sFLT-1 (AAV.shRNA.sFLT-1), as reported previously. After 4 weeks, CNV was imaged by OCT and fluorescence angiography. The scaling factors for each dimension, x, y, and z (μm/pixel) were recorded, and the corneal curvature standard was adjusted from human (7.7) to mice (1.4). The volume of each OCT image stack was calculated and then normalized by multiplying the number of voxels by the scaling factors for each dimension in Seg3D software (University of Utah Scientific Computing and Imaging Institute, available at http://www.sci.utah.edu/cibc-software/seg3d.html). Eighteen mice were prepared for choroidal flatmounts and stained by CD31. The CNV volumes were calculated using scanning laser confocal microscopy after immunohistochemistry staining. Two mice were stained by Hematoxylin and Eosin for observing the CNV morphology. Results: The CNV volume calculated using OCT was, on average, 2.6 times larger than the volume calculated using the laser confocal microscopy. The correlation statistical analysis showed OCT measuring of CNV correlated significantly with the in vitro method (R 2 =0.448, P = 0.001, n = 18). The correlation coefficient for CNV quantification using OCT and confocal microscopy was 0.693 (n = 18, P = 0.001). Conclusions: There is a fair linear correlation on CNV volumes between in vivo and in vitro methods in CNV models induced by subretinal injection. The result might provide a useful evaluation of CNV both for the studies using CNV models induced by subretinal injection and human AMD studies.

Published
2015
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3. Correlation of In Vivo and In Vitro Methods in Measuring Choroidal Vascularization Volumes Using a Subretinal Injection Induced Choroidal Neovascularization Model

Author

Balamurali K. Ambati, Juan Wang, Mao Nian Zhang, Thomas D. Olsen, Hong Wei Zhao, H. Uehara, Xiao Fei Chen, Chuang Nie, Lian Na Hu, Wen Ping Ma, Tie Shan Gao, Ling Luo, Ying Zhang, and Kyle Jackman

Subjects
medicine.medical_specialty, genetic structures, H&E stain, lcsh:Medicine, Spectral-domain Optical Coherence Tomography, law.invention, Mice, Optical coherence tomography, In vivo, Confocal microscopy, law, Ophthalmology, Choroidal Flatmount, medicine, Animals, Humans, Fluorescein Angiography, Choroidal Neovascularization, Correlation, medicine.diagnostic_test, business.industry, lcsh:R, General Medicine, Macular degeneration, Fluorescein angiography, medicine.disease, eye diseases, Mice, Inbred C57BL, Disease Models, Animal, Choroidal neovascularization, Immunohistochemistry, Optometry, Original Article, sense organs, medicine.symptom, business, Tomography, Optical Coherence
Abstract

Background: In vivo quantification of choroidal neovascularization (CNV) based on noninvasive optical coherence tomography (OCT) examination and in vitro choroidal flatmount immunohistochemistry stained of CNV currently were used to evaluate the process and severity of age-related macular degeneration (AMD) both in human and animal studies. This study aimed to investigate the correlation between these two methods in murine CNV models induced by subretinal injection. Methods: CNV was developed in 20 C57BL6/j mice by subretinal injection of adeno-associated viral delivery of a short hairpin RNA targeting sFLT-1 (AAV.shRNA.sFLT-1), as reported previously. After 4 weeks, CNV was imaged by OCT and fluorescence angiography. The scaling factors for each dimension, x, y, and z (μm/pixel) were recorded, and the corneal curvature standard was adjusted from human (7.7) to mice (1.4). The volume of each OCT image stack was calculated and then normalized by multiplying the number of voxels by the scaling factors for each dimension in Seg3D software (University of Utah Scientific Computing and Imaging Institute, available at http://www.sci.utah.edu/cibc-software/seg3d.html ). Eighteen mice were prepared for choroidal flatmounts and stained by CD31. The CNV volumes were calculated using scanning laser confocal microscopy after immunohistochemistry staining. Two mice were stained by Hematoxylin and Eosin for observing the CNV morphology. Results: The CNV volume calculated using OCT was, on average, 2.6 times larger than the volume calculated using the laser confocal microscopy. The correlation statistical analysis showed OCT measuring of CNV correlated significantly with the in vitro method ( R 2 = 0.448, P = 0.001, n = 18). The correlation coefficient for CNV quantification using OCT and confocal microscopy was 0.693 ( n = 18, P = 0.001). Conclusions: There is a fair linear correlation on CNV volumes between in vivo and in vitro methods in CNV models induced by subretinal injection. The result might provide a useful evaluation of CNV both for the studies using CNV models induced by subretinal injection and human AMD studies.

Published
2015

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