1. Two-Dimensional Liquid Chromatography
- Author
-
Nicola Marchetti, Morgan Sarrut, Sabine Heinisch, Chromatography & Hyphenated Techniques - Chromatographie et techniques couplées, Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Università degli Studi di Ferrara (UniFE), Anderson J. L., Berthod A., Pino Estévez V., and Stalcup A. M.
- Subjects
Coupling ,Chromatography ,Chemistry ,010401 analytical chemistry ,2D-LC ,analysis time ,02 engineering and technology ,comprehensive LC × LC ,dilution ,021001 nanoscience & nanotechnology ,01 natural sciences ,Method development ,NO ,0104 chemical sciences ,Dilution ,Power (physics) ,Orthogonality ,Critical parameter ,orthogonality ,[CHIM.ANAL]Chemical Sciences/Analytical chemistry ,peak capacity ,Point (geometry) ,0210 nano-technology - Abstract
Two-dimensional liquid chromatography saw significant developments in handling the need to separate a rising number of highly complex samples. 2D-LC is the coupling of the separation of a complex sample by a first LC method to a second different LC method. This chapter details the different ways to perform such coupling 2D-LC methods: heart-cutting method; offline, online, and stop-flow methods, and comprehensive LC × LC. The critical parameter is peak capacity that is defined and calculated in classical LC and 2D-LC methods. It is linked to the orthogonality of the separation power of the two coupled LC methods. From a practical point of view, the effective peak capacity is compared with the theoretical one. Analysis time and dilution are also two critical parameters in 2D-LC that need to be considered. All parameters are presented describing a practical method development. Keywords: analysis time; comprehensive LC × LC; dilution; peak capacity; orthogonality; 2D-LC
- Published
- 2015