1. Formation and abundance of 5-hydroxymethylcytosine in RNA.
- Author
-
Huber SM, van Delft P, Mendil L, Bachman M, Smollett K, Werner F, Miska EA, and Balasubramanian S
- Subjects
- 5-Methylcytosine analogs & derivatives, Animals, Chromatography, High Pressure Liquid, Cytosine biosynthesis, Cytosine chemistry, Cytosine metabolism, Mice, Mice, Inbred C57BL, Molecular Structure, Oxidation-Reduction, Tandem Mass Spectrometry, Cytosine analogs & derivatives, RNA chemistry, RNA metabolism
- Abstract
RNA methylation is emerging as a regulatory RNA modification that could have important roles in the control and coordination of gene transcription and protein translation. Herein, we describe an in vivo isotope-tracing methodology to demonstrate that the ribonucleoside 5-methylcytidine (m(5)C) is subject to oxidative processing in mammals, forming 5-hydroxymethylcytidine (hm(5)C) and 5-formylcytidine (f(5)C). Furthermore, we have identified hm(5)C in total RNA from all three domains of life and in polyA-enriched RNA fractions from mammalian cells. This suggests m(5)C oxidation is a conserved process that could have critical regulatory functions inside cells., (© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Published
- 2015
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