1. Early effector maturation of naïve human CD8 + T cells requires mitochondrial biogenesis.
- Author
-
Fischer M, Bantug GR, Dimeloe S, Gubser PM, Burgener AV, Grählert J, Balmer ML, Develioglu L, Steiner R, Unterstab G, Sauder U, Hoenger G, and Hess C
- Subjects
- CD8-Positive T-Lymphocytes immunology, Cytokines immunology, Humans, Interleukin-2 immunology, Lymphocyte Activation, Reactive Oxygen Species metabolism, CD8-Positive T-Lymphocytes cytology, Cell Differentiation immunology, Mitochondria physiology, Organelle Biogenesis
- Abstract
The role of mitochondrial biogenesis during naïve to effector differentiation of CD8
+ T cells remains ill explored. In this study, we describe a critical role for early mitochondrial biogenesis in supporting cytokine production of nascent activated human naïve CD8+ T cells. Specifically, we found that prior to the first round of cell division activated naïve CD8+ T cells rapidly increase mitochondrial mass, mitochondrial respiration, and mitochondrial reactive oxygen species (mROS) generation, which were all inter-linked and important for CD8+ T cell effector maturation. Inhibition of early mitochondrial biogenesis diminished mROS dependent IL-2 production - as well as subsequent IL-2 dependent TNF, IFN-γ, perforin, and granzyme B production. Together, these findings point to the importance of mitochondrial biogenesis during early effector maturation of CD8+ T cells., (© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)- Published
- 2018
- Full Text
- View/download PDF