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1. Genetic Engineering of Streptomyces coelicolor A3(2) for the Enantioselective Reduction of Unnatural beta-Keto-Ester Substrates.

Author

Anson CE, Bibb MJ, Booker-Milburn KI, Clissold C, Haley PJ, Hopwood DA, Ichinose K, Revill WP, Stephenson GR, and Surti CM

Abstract

Potential "reagents" for the enantioselective reduction, and other biotransformations, of beta-keto-esters result from the genetic engineering of Streptomyces coelicolor A3(2). For example, incubation of the N-acetylcysteamine thioester 1 with the recombinant strain CH999/pIJ5675 followed by treatment with MeOH/HCl gave the lactone 2 as essentially a single enantiomer.

Published
2000

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