1. Molecular and immunological characterization of Mus a 5 allergen from banana fruit.
- Author
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Aleksic I, Popovic M, Dimitrijevic R, Andjelkovic U, Vassilopoulou E, Sinaniotis A, Atanaskovic-Markovic M, Lindner B, Petersen A, Papadopoulos NG, and Gavrovic-Jankulovic M
- Subjects
- Adolescent, Adult, Amino Acid Sequence, Antigens, Plant chemistry, Cloning, Molecular, Endo-1,3(4)-beta-Glucanase immunology, Female, Food Hypersensitivity diagnosis, Food Hypersensitivity immunology, Fruit chemistry, Humans, Immunoglobulin E metabolism, Male, Molecular Sequence Data, Phosphoric Diester Hydrolases genetics, Phosphoric Diester Hydrolases metabolism, Plant Proteins chemistry, Plant Proteins immunology, Protein Isoforms chemistry, Pyrophosphatases genetics, Pyrophosphatases metabolism, Skin Tests methods, Tetraspanin 30 genetics, Tetraspanin 30 metabolism, Up-Regulation, Young Adult, Allergens immunology, Allergens isolation & purification, Antigens, Plant immunology, Antigens, Plant isolation & purification, Musa chemistry, Plant Proteins isolation & purification
- Abstract
Scope: Banana fruit has become an important cause of fruit allergy in the recent years. Among the five registered IUIS allergens, Mus a 1 and Mus a 2 have been characterized in detail. In this study, molecular characterization and evaluation of the allergenic properties of β-1,3-glucanase from banana (Musa acuminata), denoted as Mus a 5, were performed., Methods and Results: The gene of Mus a 5 was cloned and sequenced. The obtained cDNA revealed a novel Mus a 5 isoform with an open reading frame encoding a protein of 340 amino acids comprising a putative signal peptide of 28 amino acid residues. By MALDI-TOF analysis Mus a 5 isolated from banana fruit revealed a molecular mass of 33451±67 Da. Two Mus a 5 isoforms (pI 7.7 and 8.0) were detected by 2D immunoblot with an identical N-terminal sequence. By mass fingerprint, 76 and 83% of the primary structure was confirmed for the two mature Mus a 5 isoforms, respectively. IgE reactivity to Mus a 5 was found in 74% of patients sensitized to banana fruit. Upregulation of basophil activation markers CD63 and CD203c was achieved with Mus a 5 in a concentration-dependent manner., Conclusion: Mus a 5 is a functional allergen and a candidate for the component-resolved allergy diagnosis of banana allergy., (Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Published
- 2012
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