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Start Over Author "Wetzel, J. B." Publisher wiley-liss
2 results on '"Wetzel, J. B."'

1. Flow cytometric analyses of intraplant nuclear DNA content variation induced by sticky chromosomes.

Author

Rayburn AL and Wetzel JB

Subjects
Cell Nucleus genetics, Gene Expression Regulation, Plant genetics, Mitosis genetics, Soil, Triticum cytology, Triticum genetics, Zea mays cytology, Zea mays genetics, Cell Nucleus ultrastructure, Chromosome Aberrations, Chromosomes, Plant genetics, DNA, Plant genetics, Flow Cytometry methods
Abstract

Background: In several plant species, sticky chromosomes are a consequence of genetic mutations or environmental effects on mitosis and meiosis. Sticky chromosomes result in an unequal distribution of genetic material in daughter cells. This unequal distribution is hypothesized to result in an increase in the coefficient of variation (CV) of the G1 peak of dividing cells., Methods: The st1 mutant and a nonmutant line in the same genetic background of maize (Zea mays L.) were planted in a soilless mix. A wheat (Triticum aestivum L. em thell.) line was grown in both low and high aluminum-saturated soil. Both plant species were assessed for sticky chromosomes by Feulgen-stained mitotic analysis and flow cytometric analysis of propidium iodide (PI)-stained G1 nuclei., Results: In the st1 mutant, a significant increase in the number of abnormal anaphase figures was observed. An increase in abnormal mitotic figures was observed in wheat plants grown in aluminum soil. Using flow cytometry, an increase in the CV of the G1/G0 peak was seen in the maize mutant and in wheat grown at high levels of aluminum saturation. This increase correlated with the number of abnormal anaphase cells observed., Conclusions: Flow cytometry was sensitive enough to detect the intraplant nuclear DNA variation associated with sticky chromosomes within a plant., (Copyright 2002 Wiley-Liss, Inc.)

Published
2002
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2. Use of fluorescence genomic in situ hybridization (GISH) to detect the presence of alien chromatin in wheat lines differing in nuclear DNA content.

Author

Wetzel JB and Rayburn AL

Subjects
Cell Nucleus genetics, Chromosomes, DNA, Plant analysis, Genome, Plant, Chimera genetics, Chromatin genetics, Flow Cytometry methods, In Situ Hybridization, Fluorescence methods, Triticum genetics
Abstract

Background: Many times small differences in genome size are reported between or within plant species in which no cytologic confirmation is made. Attempts to repeat these studies have met with limited success. The controversy then becomes whether or not these small differences that were not confirmed cytologically are real. The present study was undertaken to determine if the approximately 1% nuclear variation seen by flow cytometry among wheat lines selected for aluminum response was due to actual chromatin differences., Methods: The three parental wheat cultivars used in the aluminum selection along with the isolines resulting from the selection were analyzed. One parental line had previously been reported to have alien chromatin substituted for the corresponding wheat homologous chromatin. Genomic in situ hybridization was used to determine the presence or absence of rye chromatin in three cultivars and six near-isolines of wheat., Results: Upon observing metaphase chromosomes of the Century parent and its isolines, two of the chromosomes were observed to be one-half yellow-orange, indicating rye chromatin with the remaining portion of the chromosomes and the other 40 wheat chromosomes having no label indicating wheat chromatin. In the Chisholm parent and its isolines, none of the chromosomes were labeled, indicating the absence of rye chromatin. In addition, none of the third parents' chromosomes had the rye yellow-orange signal., Conclusions: The wheat lines with the larger DNA contents were observed to have alien DNA present. DNA differences between the normal wheat chromosomes and the substituting alien chromatin were calculated based on total chromosome length. The increase in genome size of the wheat lines containing the alien chromatin appears to be the result of the alien chromatin having approximately 43% more DNA than the wheat chromatin it is replacing. Thus, the small DNA difference previously reported by flow cytometry was demonstrated to be a real DNA variation due to the presence of small fragments of alien chromosomes added to the wheat genome., (Copyright 2000 Wiley-Liss, Inc.)

Published
2000

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