1. Temporal regulation of Cre-recombinase activity in Scl-positive neurons of the central nervous system.
- Author
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Bradley CK, Takano EA, Göthert JR, Göttgens B, Green AR, Begley CG, and van Eekelen JA
- Subjects
- Animals, Central Nervous System embryology, Mice, Mice, Inbred C57BL, Mice, Transgenic, Models, Animal, Recombination, Genetic, Tamoxifen administration & dosage, Time Factors, Tissue Distribution, Central Nervous System enzymology, Gene Expression Regulation, Developmental, Integrases metabolism, Neurons enzymology, Tamoxifen metabolism
- Abstract
The Cre/LoxP system provides a powerful tool to investigate gene function in vivo. This system requires Cre-recombinase expressing mouse lines that permit control of gene recombination in a tissue-specific and time-dependent manner. To allow spatio-temporal gene deletion in specific central nervous system (CNS) neuronal populations, we generated mice with a tamoxifen-inducible Cre (Cre-ER(T)) transgene under control of the Scl/Tal1 neural promoter/enhancer -0.9E3 (-0.9E3CreER(T) transgenic mice). Using Cre-reporter mice we have shown that tamoxifen-mediated Cre-ER(T) recombination in -0.9E3CreER(T) mice recapitulated the anticipated expression pattern of Scl in the caudal thalamus, midbrain, hindbrain, and spinal cord. Cre-mediated recombination was also effectively induced during embryogenesis and marked the same population of neurons as observed in the adult. Additionally, we identified a tamoxifen-independent constitutively active -0.9E3CreER(T) mouse line that will be useful for gene deletion during early neurogenesis. These -0.9E3CreER(T) mice will provide tools to investigate the role of neuronal genes in the developing and mature CNS. CNS., ((c) 2007 Wiley-Liss, Inc.)
- Published
- 2007
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