1. Assessing iPSC reprogramming methods for their suitability in translational medicine.
- Author
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Rao MS and Malik N
- Subjects
- Genetic Vectors genetics, Genetic Vectors metabolism, Induced Pluripotent Stem Cells metabolism, Lentivirus genetics, Lentivirus metabolism, MicroRNAs genetics, MicroRNAs metabolism, Plasmids genetics, Plasmids metabolism, RNA, Messenger genetics, RNA, Messenger metabolism, Regenerative Medicine, Reproducibility of Results, Transfection, Transgenes, Cell Differentiation, Cell Engineering methods, Induced Pluripotent Stem Cells cytology, Translational Research, Biomedical methods
- Abstract
The discovery of the ability to induce somatic cells to a pluripotent state through the overexpression of specific transcription factors has the potential to transform the ways in which pharmaceutical agents and cellular transplantation therapies are developed. Proper utilization of the technology to generate induced pluripotent stem cells (iPSCs) requires that researchers select the appropriate reprogramming method for generating iPSCs so that the resulting iPSCs can be transitioned towards clinical applications effectively. This article reviews all of the currently available reprogramming techniques with a focus on critiquing them on the basis of their utility in translational medicine., (Copyright © 2012 Wiley Periodicals, Inc. This article is a U.S. Government work and is in the public domain in the USA.)
- Published
- 2012
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