1. Potassium-induced reverse transformation of cells infected with a temperature-sensitive transformation mutant virus.
- Author
-
Lai CN and Becker FF
- Subjects
- Animals, Cell Differentiation, Cells, Cultured, Culture Media, Hypertonic Solutions, Moloney murine sarcoma virus genetics, Moloney murine sarcoma virus physiology, Mutation, Osmolar Concentration, Phenotype, Rats, Sarcoma, Experimental pathology, Cell Transformation, Viral drug effects, Kidney pathology, Potassium pharmacology, Sarcoma, Experimental genetics, Temperature
- Abstract
High potassium concentrations altered the morphology and the ability to grow in soft agar in 6m2 cells, a clone of rat kidney cells infected with a temperature-sensitive mutant of Moloney sarcoma virus. Approximately 60% of cells exhibited normal morphology in the presence of 94.8 mM potassium in isotonic medium at the temperature permissive for transformation, whereas 100% were normal at 72 mM potassium in hypertonic media. A significant reduction of growth in soft agar was also induced with these conditions. However, the synthesis ratio of virus-specified transforming protein to marker viral protein was not altered. Na+K+-ATPase might play a role in this reverse-transformation process.
- Published
- 1985
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