1. Wnt3a is involved in the early stage of miPSC and mESC haemopoietic differentiation.
- Author
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Zhang, Wencheng, Yao, Hailei, Wang, Sihan, Shi, Shuangshuang, Lv, Yang, He, Lijuan, Nan, Xue, Yue, Wen, Li, Yanhua, and Pei, Xuetao
- Subjects
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CELLULAR signal transduction , *EMBRYONIC stem cells , *INDUCED pluripotent stem cells , *CELL differentiation , *CELL culture , *CELL lines , *LABORATORY mice - Abstract
The Wnt/β-catenin signalling pathway is important in regulating not only self-renewal of haemopoietic progenitors and stem cells but also haemopoietic differentiation of ESCs (embryonic stem cells). However, it is still not clear how it affects haemopoietic differentiation. We have used a co-culture system for haemopoietic differentiation of mouse ESCs and iPSCs (induced pluripotent stem cells) in which the Wnt3a gene-modified OP9 cell line is used as stromal cells. The number of both Flk1+ and CD41+ cells generated from both co-cultured mouse ESCs and mouse iPSCs increased significantly, which suggest that Wnt3a is involved in the early stages of haemopoietic differentiation of mouse ESCs and mouse iPSCs in vitro. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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