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Although statistical analysis of our material could indicate that PRV-1 infection is associated with red muscle melanization, other infections or even co-infections with SAV2 or PMCV may have contributed to the development of the condition. All affected samples were positive for PRV-1 with a median I C i SB t sb value of 30.2, for the control fish, four samples were PRV-1 positive, and two were negative. Keywords: filet; melanin; melano-macrophage; piscine myocarditis virus; piscine orthoreovirus 1; salmonid alphavirus EN filet melanin melano-macrophage piscine myocarditis virus piscine orthoreovirus 1 salmonid alphavirus 453 458 6 03/10/23 20230401 NES 230401 INTRODUCTION Farmed Atlantic salmon ( I Salmo salar i L.) are susceptible to melanization of the white muscle ("the filet"), where melanized spots have a prevalence of 20%-30% at slaughter (Mørkøre et al., [16]). Melanized focal changes in skeletal muscle in farmed Atlantic salmon after natural infection with Piscine orthoreovirus (PRV). [Extracted from the article]