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Chronic activation of the IL-1β system in adipose tissue on metabolic disorders is well demonstrated. However, a mechanism for its expression and activation in the tissue has remained unexplored. Here, we demonstrate that IL-1β transcript was enriched in neutrophils of white adipose tissue (WAT) from lean mice. Mechanistically, the interaction of neutrophils with adipocytes induced IL-1β expression via NF-κB pathway. Lipolysis of adipocytes accumulated neutrophils prior to macrophages in WAT and produced high levels of IL-1β via an inflammasome pathway. Leukotriene B4 (LTB4) production in WAT also contributed to neutrophil accumulation. Furthermore, an LTB4-inflammasome axis contributed to the expression of chemotactic molecules involved in high-fat diet-induced macrophage infiltration into WAT. We have identified previously unappreciated roles for neutrophils in the development of adipose tissue inflammation: robust IL-1β production and infiltration of macrophages to initiate chronic inflammation. [ABSTRACT FROM AUTHOR]

Permeability of a streambed is an important factor regulating nutrient and oxygen availability for aquatic biota. In order to investigate the relationship, an accurate permeability should be measured. However, it is difficult to measure permeability in a coarse gravel bed using a conventional permeability test. Moreover, turbulent flow may occur in coarse bed material, and then deviations from Darcy's law do occur. Thus, permeability calculated following Darcy's law may be overestimated under turbulent flow conditions and should be corrected. The packer test can be used in highly permeable gravel beds. We developed a field method applicable to a gravel bed using the packer test and derived an equation adopting a law of turbulent flow to study the problems under any type of flow condition. The accuracy of the equation was examined using a laboratory flume with a gravel bed. The results suggested that permeability calculated from Hvorslev's equation is overestimated for turbulent flow. In contrast, our equation, developed here, could evaluate permeability accurately under any type of flow condition. Copyright © 2005 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]