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Backgrounds: The role of cytoreductive radical prostatectomy (cRP) for bone‐metastatic prostate cancer (bmPCa) remains controversial. We aimed to figure out whether cRP and lymph node dissection (LND) can benefit bmPCa. Methods: 11,271 PCa patients with bone metastatic burden from 2010 to 2019 were identified using SEER‐Medicare. Overall survival (OS) and cancer‐specific survival (CSS) rates were visualized using Kaplan–Meier plots. Multivariable Cox regression analyses were constructed to examine the effects of cRP and LND on survival, after stratifying to age, prostate specific antigen (PSA), clinical stages, Gleason score, metastatic burden, radiotherapy, and chemotherapy status. Results: 317 PCa patients underwent cRP and cRP was increasingly performed for bmPCa from 2010 (2.2%) to 2019 (3.0%) (p < 0.05). In multi analyses, cRP was predisposed to a better OS or CSS in patients with age < 75, PSA < 98 ng/mL, bone‐only metastatic sites or patients not receiving chemotherapy (all p < 0.05). For the patients undergoing cRP, LND especially extended LND was associated with a better OS or CSS (all p < 0.05). Conclusions: cRP might benefit OS or CSS in young patients with low PSA and bone‐only metastatic sites not receiving chemotherapy. And a clear OS or CSS benefit of LND especially extended LND was observed in patients undergoing cRP. [ABSTRACT FROM AUTHOR]

Objective: Kawasaki disease (KD) can lead to permanent damage to coronary structures, the pathogenesis of which remains unknown. This experiment was designed to investigate whether miR‐223‐3p secreted in the serum of KD patients affects the proliferation and apoptosis of HCAECs in KD by regulating FOXP3. Methods: Blood samples were collected in acute febrile phase of KD, after IVIG treatment, and from healthy controls. Transfected into HCAECs cells by synthetic FOXP3 siRNA/NC. A co‐culture system was established between HCAECs cells transfected with FOXP3 siRNA/NC and THP1 cells added with three sera. Results: Compared with the control group, the expressions of miR‐223‐3p, RORγt, and Th17 in serum of KD patients were significantly upregulated, and the expressions of TGF‐β1, FOXP3 and Treg were significantly downregulated. At the same time, the levels of IL‐6, IL‐17, and IL‐23 were significantly increased, and the levels of IL‐10 and FOXP3 were significantly decreased. After IVIG treatment, the patient's above results were reversed. The serum of KD patients increased the expression of miR‐223‐3p and inhibited the expression of FOXP3 in HCAECs cells. IVIG serum is the opposite. Overexpression of miR‐223‐3p also promoted the apoptosis of HCAECs. In addition, serum from KD patients promoted apoptosis, whereas serum after IVIG treatment inhibited apoptosis. KD patient serum downregulated the expression of FOXP3, Bcl2, TGF‐β1 and IL‐10 in cells, and upregulated the expression of caspase3, Bax, IL‐17, IL‐6, and IL‐23. The opposite results were obtained with IVIG‐treated sera. Conclusion: miR‐223‐3p secreted in serum of KD patients can regulate the expression of FOXP3 and affect the proliferation, apoptosis, and inflammation of cells. [ABSTRACT FROM AUTHOR]

Objective: To describe the surgical technique of cervical transdural discectomy with laminoplasty (CTDL) for the treatment of multi‐segment cervical spinal stenosis (CSS) accompanied with cervical disc herniation (CDH) and investigate its surgical outcomes and complications. Methods: This was a clinical study. Between 2012 and 2018, 31 patients (13 males and 18 females) with multi‐segment CSS (over two cervical segments) accompanied with huge CDH and underwent CTDL were enrolled in this study. The details of CTDL technique with general anesthesia was described by the authors. The average follow‐up period of patients was 65.03 months (range from 24 to 126 months). Perioperative parameters such as age, sex, operative level, operative time, estimated blood loss, ambulation time, and operative complications were recorded. The results of clinical metrics such as the visual analog scale (VAS) and Japanese Orthopaedic Association (JOA) scores in the preoperative and during the follow‐up period were obtained and used to evaluate clinical outcomes. Radiographic improvement was evaluated by the compression ratio, sagittal maximum spinal cord compression (SMSCC), and cervical range of motion (ROM). The preoperative and postoperative follow‐up parameters (VAS, JOA, Compression ratio, SMSCC, and ROM) were assessed with paired t test. A P‐value <0.05 was considered statistically significant. Results: In the study, the mean age of the 31 patients was 55.23 ± 10.97 years. The mean operative time was 192.45 ± 24.17 min (ranging from 150 to 245 min), and intraoperative blood loss was 322.58 ± 129.00 mL (ranging from 150 to 600 mL). The VAS neck pain was improved significantly over the follow‐up period (P < 0.05, respectively). The VAS arm pain improved significantly from 6.26 ± 0.93 preoperatively to 1.74 ± 0.63 at 24 months postoperatively (P < 0.001). There was no significant difference in improvement of VAS arm pain between 24 months postoperatively and final follow‐up (P = 0.180). Compared with preoperative JOA score, JOA score was significantly improved at 24 months postoperatively (14.79 ± 1.84 vs 9.66 ± 2.81, P < 0.001). Meanwhile, there were no statistically significant differences between the final follow‐up and the postoperative JOA scores (15.08 ± 1.71 vs 14.79 ± 1.84, P = 0.051). Postoperative patients showed significantly higher index of compression ratio (58.30 ± 8.51 vs 27.17 ± 3.89, P < 0.001) and lower SMSCC (25.12 ± 5.67 vs 33.66 ± 5.38, P < 0.001). In addition, there was no significant difference between preoperative and postoperative cervical ROM (P = 0.740). One patient observed postoperative symptom of C6 nerve root injury, which was resolved within 24 months after the surgery; meanwhile, the neurological monitoring also reflected the intraoperative stretching of the C6 nerve root. Two cases involved postoperative cerebrospinal fluid (CSF) leakage which may have been related to laceration of dura mater. Conclusions: This study suggested that CTDL technique could acquire satisfactory surgical outcomes for patients with multi‐segment CSS accompanied with CDH, but the surgical indications of the patients need to be selected strictly. [ABSTRACT FROM AUTHOR]

Cloud phase (CP) partitioning (e.g., supercooled water cloud fraction, SCF) is often misrepresented in model simulations. One of the remarkable uncertainties involves the poor understanding of SCF diurnal cycle due to lack of observations. By using 33 months of lidar observations from Cloud‐Aerosol Transport System which provides cloud top phase at different local times for a given grid and isotherm, this study first characterizes the diurnal cycle of SCF between 51°S and 51°N. Distinct SCF diurnal variations have been found near‐global at different isotherms, especially for −22.5°C, where SCF diurnal ranges even reach 60%. Correlation analysis shows a robust dependence of SCF diurnal cycle on the variation of dust aerosol extinction coefficient instead of other dust load indicators and meteorological parameters. Given the important roles of SCF on the radiation budget, this study will help us better constrain aerosol‐cloud interaction and simulations of CP partitioning in models. Plain Language Summary: Clouds in nature are comprised of liquid droplets, ice crystals or a mixture of the two (i.e., mixed‐phase) at temperatures between −40°C and 0°C. Clouds with different cloud phases (CPs) exhibit distinct radiative effect due to differences of cloud liquid and ice in refractive indices, sizes, concentrations and shapes. However, CP partitioning (e.g., supercooled water cloud fraction, SCF) is still misrepresented in model simulations. One crucial reason is the lack of observations and understanding of SCF diurnal variations. This study first characterizes the SCF diurnal variations by using ∼33 months of lidar observations from Cloud‐Aerosol Transport System which provides cloud top phase at different daily times for a given grid and isotherm. Distinct SCF cycle has been found globally at different isothermal layers with a minimum (maximum) SCF around midday (midnight), which is mainly related with the diurnal cycle of dust aerosols extinction coefficient (EC). Given the important roles of SCF on the radiation budget, this work suggests that EC may be considered as the robust dust load indicator to study aerosol‐cloud interaction and further improve the simulations of CP partitioning in climate models. Key Points: Distinct supercooled water cloud fraction (SCF) diurnal variations have been found near‐global at different isothermal layersThe SCF reaches a minimum (maximum) around midday (midnight)Compared with other parameters, the dust extinction coefficient is highly correlated with SCF diurnal cycle [ABSTRACT FROM AUTHOR]

Objective: To evaluate the clinical outcome of manual reduction combined with pedicle fixation through Wiltse paraspinal approach (WPA) in the treatment of thoracolumbar fractures. Methods: From May 2017 to May 2019, 48 thoracolumbar fractures patients without neurological symptoms were enrolled in this study. Forty‐eight patients were randomly divided into two groups based on the different surgical treatment. Group 1 was manual reduction combined with pedicle screw fixation through Wiltse paraspinal approach treatment group. Group 2 was pedicle screw fixation through traditional posterior approach treatment group. The operation time (OT), intraoperative blood loss (BL), postoperative drainage (PD), time of brace (TB) and the cobb angle recovery of the injured kyphosis in the prone position were obtained and compared between the two groups, respectively. Comparison of cobb angle changes, serum creatine kinase (CK) level, pain visual analogue score (VAS), Oswestry disability index (ODI), and multifidus cross‐sectional (MCS) area changes were achieved between the two groups, respectively. Results: Forty‐eight patients were enrolled in this study and each group had 24 patients. There was no significant difference between the two groups in patient's age, height, weight, and body mass index (BMI). There were 20 males and four females in group 1. The mean age, height, weight, and BMI of patients were 61.99 ± 11.00 years (range, 42–75 years), 175.21 ± 4.49 cm, 76.71 ± 4.87 kg, and 24.98 ± 1.03 kg/m2 in group 1, respectively. Group 2 had 18 males and six females, and the mean age, height, weight, and BMI of patients were 57.95 ± 9.22 years (range, 44–77 years), 176.37 ± 4.56 cm, 77.42 ± 4.61 kg, and 24.87 ± 1.10 kg/m2 in group 2, respectively. The mean bleeding volume of group 1 was significantly less than group 2 (64.13 ± 9.77 ml and 152.13 ± 10.73 ml, respectively) (P < 0.05). The mean operation time, postoperative drainage, and time of brace were 62.95 ± 9.80 min, 66.25 ± 12.75 ml, and 3.62 ± 0.97 days in group 1, respectively, and they were significantly better than those of group 2 (69.29 ± 6.82min, 162.96 ± 14.55ml and 7.88 ± 1.94 days, respectively) (P < 0.05). The mean multifidus cross‐sectional area was significantly smaller than per‐operation after surgery in two groups (P < 0.05). The mean creatine kinase of group 1 was 403.13 ± 39.78 U/L and 292.12 ± 45.81 U/L at 1 and 3 days after surgery, respectively, which was significantly smaller than those in group 2 (654.25 ± 53.13 U/L and 467.67 ± 44.25 U/L, respectively) (P < 0.05). The Oswestry disability index of group 1 were significantly better than those in group 2 especially at 1 month and final follow‐up after surgery (P < 0.05). Moreover, group 1 also had better outcomes in postoperative Cobb angle change than those in group 2, with significant difference on intra‐operation, 1 day and 1 month post‐operation (P < 0.05). Conclusion: In short, this operation is suitable for thoracolumbar fractures without neurologic symptoms. Preoperative manual reduction had advantages of restoring the height of injured vertebrae. Wiltse intermuscular approach can reduce intraoperative blood loss, shorten operation time, and reduce paraspinal muscle damage. Using the traditional posterior approach, it is easy for surgeons to grasp this technique and it should be recommended as conforming with the minimally invasive approach of recent years. [ABSTRACT FROM AUTHOR]

Studies have found that salidroside, isolated from Rhodiola rosea L, has various pharmacological activities, but there have been no studies on the effects of salidroside on brain hippocampal senescence. The purpose of this study was to investigate the mechanistic role of salidroside in hippocampal neuron senescence and injury. In this study, long-term cultured primary rat hippocampal neurons and naturally aged C57 mice were treated with salidroside. The results showed that salidroside increased the viability and MAP2 expression, reduced β-galactosidase (β-gal) levels of rat primary hippocampal neurons. Salidroside also improved cognition dysfunction in ageing mice and alleviated neuronal degeneration in the ageing mice CA1 region. Moreover, salidroside decreased the levels of oxidative stress and p21, p16 protein expressions of hippocampal neurons and ageing mice. Salidroside promoted telomerase reverse transcriptase (TERT) protein expression via the phosphatidylinositol-3-kinase (PI3K)/protein kinase B (Akt) pathway. In conclusion, our findings suggest that salidroside has the potential to be used as a therapeutic strategy for anti-ageing and ageing-related disease treatment. [ABSTRACT FROM AUTHOR]

Although the role of bromodomain‐containing protein 4 (BRD4) in ovarian cancer, pancreatic cancer, lymphoma, and many other diseases is well known, its function in cutaneous melanoma is only partially understood. The results of the present study show that the BRD4 inhibitor JQ1 promotes the apoptosis of B16 melanoma cells by altering mitochondrial dynamics, thereby inducing mitochondrial dysfunction and increasing oxidative stress. We found that treatment of B16 cells with different concentrations of JQ1 (125 nmol/L or 250 nmol/L) significantly downregulated the expression of protein subunits involved in mitochondrial respiratory chain complexes I, III, IV, and V, increased reactive oxygen species, induced energy metabolism dysfunction, significantly enhanced apoptosis, and activated the mitochondrial apoptosis pathway. At the same time, JQ1 inhibited the activation of AMP‐activated protein kinase, a metabolic energy sensor. In addition, we found that the mRNA and protein levels of mitochondrial dynamin‐related protein 1 increased, whereas the levels of mitochondrial fusion protein 1 and optic atrophy protein 1 decreased. Mechanistically, we determined that JQ1 inhibited the expression of c‐Myc and altered mitochondrial dynamics, eventually leading to changes in the mitochondrial function, metabolism, and apoptosis of B16 melanoma cells. [ABSTRACT FROM AUTHOR]

Melanoma is one of the most aggressive skin cancers. Existing evidence has reported the aberrant expression of microRNAs (miRNAs) in melanoma, but their putative targets and underlying downstream effects remain to be further understood. Herein, we explored the suppressive role of miR‐485‐5p in melanoma progression. Initial bioinformatics analyses showed that the PRRX1 gene was differentially expressed in melanoma, while miR‐485‐5p was predicted to be a potential regulatory miRNA binding to PRRX1 mRNA. We confirmed that PRRX1 was upregulated, while miR‐485‐5p was downregulated in human melanoma samples compared with adjacent normal skin tissues. We then showed that PRRX1 was a target gene of miR‐485‐5p by dual‐luciferase reporter gene assay. Moreover, a reduction in the expression of PRRX1 and downregulation of important proteins of the transforming growth factor‐beta (TGFβ) signaling pathway was observed after miR‐485‐5p overexpression. Furthermore, miR‐485‐5p overexpression or PRRX1 knockdown suppressed epithelial–mesenchymal transition, cell viability, migration, and invasion, and promoted cell apoptosis in melanoma cells. Our study demonstrates the tumor‐suppressive functions of miR‐485‐5p in the development of human melanoma, providing a potential target for therapy. [ABSTRACT FROM AUTHOR]

Cerebroprotein hydrolysate‐I (CH‐I)，a mixture of peptides extracted from porcine brain tissue，has shown a neuroprotective effect, but its role in brain senescence is unclear. In the present study, we established a senescence model of PC12 cells and mice to investigate the effect of CH‐I on brain senescence via JAK2/STAT3 pathway. The results showed that CH‐I could improve cell viability, inhibit the apoptosis of cells, and reduce the senescence‐positive cells induced by D‐galactose. In vivo, CH‐I improved the learning ability and memory of aging mice, reduced neuronal damage in mice hippocampus. Mechanism studies showed that CH‐I could adjust BDNF protein expressions, activate JAK2/STAT3 pathway, and finally enhance telomerase activity. All these findings indicated that CH‐I showed a neuroprotective effect against brain senescence. These results might provide further reference and support for the application of CH‐I in delaying aging. [ABSTRACT FROM AUTHOR]

Since Charles Darwin discovered growth movements of the shoot tip (coleoptile) toward light, the grass coleoptiles have long been used as a model system to study plant growth. Rapid growth of the coleoptile is vital for successful seed germination and early seedling establishment. However, the proteome changes underlying the rapid growth of the coleoptiles are not yet clear in the model plant maize (Zea mays). In the present study, we investigated proteome changes in vivo occurring in the rapidly growing coleoptiles of maize with two‐dimensional gel electrophoresis combined with mass spectrometry. A quantitative comparison of the proteomes at 1.5, 3, and 5 days after germination showed significant changes in protein profiles with coleoptile growth. As a result, 31 differential abundance proteins (DAPs) representing 44 protein spots were identified, of which 21 DAPs with increased abundances were implied in growth‐related processes, including translational initiation, transcription regulation, protein and other compound synthesis, H+‐transmembrane transport and cytoskeleton organization. The selected DAPs were confirmed by reverse transcription quantitative PCR and immunoblot analysis. We suggested that the rapid growth of the coleoptile is largely due to its ability to quickly enhance relevant cellular processes, especially the increased synthesis of the growth‐related DAPs. The content of indole‐3‐acetic acid, salicylic acid, and jasmonic acid decreased significantly, and the content of gibberellins first decreased and then increased during the elongation of coleoptile. This study provides new insight into the significance of proteome changes in coleoptile growth. [ABSTRACT FROM AUTHOR]

Aerosols can affect crop photosynthesis by altering radiation and meteorological conditions. By combining field observations, mechanistic modeling, and satellite‐retrieved solar‐induced chlorophyll fluorescence (SIF), we assessed aerosols' impacts on crop photosynthesis from leaf to regional scale. We found that the initial increase in aerosol optical depth (AOD) enhanced photosynthesis of sun leaves, shade leaves, and canopy, which reached their maximum at AOD = 0.76, 1.13, and 0.93, respectively, and then decreased. Aerosol‐induced changes in radiation regime and the concurrent high relative humidity led to such nonlinear responses. Similarly, the SIF of croplands in the North China Plain (NCP) also showed a bell‐shaped response to aerosols. The optimal AOD level at which SIF reached the maximum value varied from 0.56 to 1.04, depending on the background meteorological conditions. Approximately 76%–90% of the NCP exceeded the optimal AOD level, suggesting that stringent aerosol pollution control could promote cropland productivity in this region. Plain Language Summary: High aerosol loading could produce either a positive or negative impact on crop photosynthesis. However, experimental tools for manipulating aerosol loading in the field are lacking, creating significant uncertainty in assessing the impact of aerosols on crop yields. The cyclical fluctuations in aerosol loading in the North China Plain (NCP) provide a unique opportunity to study aerosols' effect on crop productivity. We found that the photosynthesis of sun and shade leaves and canopy reached their maximum at a moderate level of aerosol loading. Similarly, satellite‐observed solar‐induced chlorophyll fluorescence across the NCP also demonstrated that intermediate aerosols enhance crop photosynthesis at regional scales. The further analysis indicated that the changes in direct and diffuse radiation and the concurrent high air humidity together led to the nonlinear response of soybean photosynthesis to aerosol loading. Our findings suggested that stringent aerosol pollution control will increase crop productivity in the NCP. Key Points: Soybean sun and shade leaf photosynthesis show a quadratic response to aerosols, peaking at aerosol optical depth of 0.76 and 1.13, respectivelyThe changes in radiation regimes and concurrent high relative humidity lead to such nonlinear responses of leaf photosynthesisSolar‐induced chlorophyll fluorescence in the North China Plain also demonstrate that intermediate aerosols enhance crop photosynthesis [ABSTRACT FROM AUTHOR]

The clear‐sky aerosol direct radiative effect (DRE) was estimated at the Atmospheric Radiation Measurement (ARM) Southern Great Plains (SGP) and Tropical Western Pacific (TWP) sites. The NASA Langley Fu‐Liou radiation model was used with observed inputs including aerosol vertical extinction profile from the Raman lidar; spectral aerosol optical depth (AOD), single‐scattering albedo and asymmetry factor from Aerosol Robotic Network; temperature and water vapor profiles from radiosondes; and surface shortwave (SW) spectral albedo from radiometers. A radiative closure experiment was conducted for clear‐sky conditions. The mean differences of modeled and observed surface downwelling SW total fluxes were 1 W m−2 at SGP and 2 W m−2 at TWP, which are within observational uncertainty. At SGP, the estimated annual mean clear‐sky aerosol DRE is −3.00 W m−2 at the top of atmosphere (TOA) and −6.85 W m−2 at the surface. The strongest aerosol DRE of −4.81 (−10.77) W m−2 at the TOA (surface) are in the summer when AODs are largest. The weakest aerosol DRE of −1.28 (−2.77) W m−2 at the TOA (surface) are in November–January when AODs and single‐scattering albedos are lowest. At TWP, the annual mean clear‐sky DRE is −2.82 W m−2 at the TOA and −10.34 W m−2 at the surface. The strongest aerosol DRE of −5.95 (−22.20) W m−2 at the TOA (surface) are in November (October) due to the biomass burning season's peak. The weakest aerosol DRE of −0.96 (−4.16) W m−2 at the TOA (surface) are in March (April) when AODs are smallest. Key Points: Clear‐sky aerosol direct radiative effects (DRE) were estimated based on multiyear ground‐based observations at the Atmospheric Radiation Measurement Southern Great Plains (SGP) and Tropical Western Pacific (TWP) sitesModeled clear‐sky surface downwelling shortwave fluxes using observed inputs agreed well with surface radiometer observationsThe annual mean clear‐sky aerosol DRE at the top of the atmosphere are −3.00 W m−2 at SGP and −2.82 W m−2 at TWP [ABSTRACT FROM AUTHOR]

Objective: To study the distribution of sympathetic nerves of the ligamentum flavum (LF), confirm its existence by histological observation and nuclear magnetic resonance spectroscopy, and analyze the relationship between sympathetic nerve fibers and the biomechanical structure of the LF. Methods: Randomly controlled scientific research selected 15 cases of posterior surgery in the affiliated hospital of Qingdao University from January 2013 to December 2019. The average age was 67.5 ± 14.5 years old, eight males and seven females. The LF specimens (completely separated fresh tissue) of different segments (C3‐7) were taken during the operation. Two pages of LF specimens on the left and right sides of the same segment are randomly allocated by the pairing method for formalin fixation and cryopreservation in liquid nitrogen. LF specimens extracted from seven other adult cadaver specimens (average age at death of about 56.8 ± 4.0 years, three males and four females) were used as a control group; together with formalin‐ fixed specimens obtained during surgery, 3D slices were given layer by layer. The distribution of sympathetic nerves in different parts of the LF was analyzed by glyoxylic acid‐induced biological monoamine fluorescent technique (SPG) and hematoxylin–eosin (HE) staining. Fifteen liquid nitrogen storage specimens were divided into the back of the LF and the spinal canal through frozen sections, and were analyzed by nuclear magnetic resonance spectroscopy‐hydrogen spectrum (1H ‐NMR) for neurotransmitters and neurometabolites. Results: There were type C sympathetic nerve fibers in the LF, which were divided into linear shape (α) and wave shape (β). Experimental group (χ2 = 1.705, P > 0.05) and control group (χ2 = 0.879, P > 0.05) can detect no difference in fluorescence units. Nerve fiber transmitter metabolites choline (Cho), creator (Cr), γ‐aminobutyric acid (GABA) also indicate that the sympathetic nerve is present in the LF. LF sympathetic nerve fibers were mainly distributed in the proximal spinal canal surface, nerve fibers on the medial belt (area II) were fewer than the lateral belt (area I) (W = 210, P < 0.05). The 1HNMR spectrum of LF spinal canal PG / Cho (t = 8.721, P < 0.05), GABA (t = 16.01, P < 0.05) value increased, lactic acid (Lac) / Cr (t = 4.213, P < 0.05), Cho / Cr (t = 2.402, P < 0.05) value decreased, indicating that nerve fibers are actively metabolized on the surface of the spinal canal, mainly distributed in tube surface. βtype fibers were more often distributed around microvessels. A small amount of α type fibers went next to the vascular structures, while α type fibers and β type fibers go cross within LF. Two patients with vertebral artery dissection had no recurrence of sympathetic symptoms within a total of 12 follow‐ups 2 years after discharge. Conclusions: There are many sympathetic nerve fibers distributed on LF, and their distribution may be correlated with histological and mechanical characteristics of LF. It may also be the anatomical basis of cervical vertigo. [ABSTRACT FROM AUTHOR]

Osteosarcoma (OS) is the most common primary malignant bone tumor in children and adolescents, with a high metastatic potential. Despite dramatic changes in OS treatments over the past decades, their efficiency still remains limited, with severe complications and adverse side effects. Key mechanisms underlining tumorigenesis, metastasis and chemotherapy resistance are currently lacking, in turn hindering any progress with respect to developing effective and safe therapeutic strategies against OS. Recently, ADAMTS7, a member of the disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) family, was shown to be involved in osteogenic differentiation‐related pathological processes. ADAMTS7 promotes vascular calcification via disturbing the balance between osteogenic bone morphogenetic protein (BMP)2 (regulating osteogenic differentiation and bone formation during development) and its natural inhibitor cartilage oligomeric matrix protein (Comp). Hence, in the present study, we aimed to investigate the role of ADAMTS7 in the pathological process of OS. We first revealed that ADAMTS7 was decreased in OS tissues. Lower expression of ADAMTS7 was correlated with poor histological differentiation and an advanced clinical stage of OS. Through loss‐ and gain‐function analysis, we further revealed that ADAMTS7 attenuated cell proliferation, migration and invasion, at the same time as promoting the expression of osteogenic differentiation markers in two OS cell lines: MG63 and SAOS2. Moreover, Comp was responsible for the effects of ADAMTS7 on OS pathogenesis by reinforcing cell osteogenic differentiation mediated by BMP2 in vitro. In conclusion, ADAMTS7‐mediated degradation of Comp may provide a potential therapeutic target for the treatment of OS. [ABSTRACT FROM AUTHOR]

Summary: The uncomposted faeces of dairy cow are usually stacked on cow breeding farms, dried under natural conditions and then used as cow bedding material or they may be continuously piled up. However, no information is available to evaluate variations in the human and animal pathogen genes and antibiotic resistance during the accumulation of fresh faeces of dairy cow to manure. Here, we present the metagenomic analysis of fresh faeces and manure from a dairy farm in Ning Xia, showing a unique enrichment of human and animal pathogen genes and antibiotic resistance genes (ARGs) in manure. We found that manure accumulation could significantly increase the diversity and abundance of the pathogenic constituents. Furthermore, pathogens from manure could spread to the plant environment and enphytotic pathogens could affect the yield and quality of crops during the use of manure as a fertilizer. Levels of virulence genes and ARGs increased with the enrichment of microbes and pathogens when faeces accumulated to manure. Accumulated manure was also the transfer station of ARGs to enrich the ARGs in the environment, indicating the ubiquitous presence of environmental antibiotic resistance genes. Our results demonstrate that manure accumulation and usage without effective manure management is an unreasonable approach that could enrich pathogenic microorganisms and ARGs in the environment. The manure metagenome structure allows us to appreciate the overall influence and interaction of animal waste on water, soil and other areas impacted by faecal accumulation and the factors that influence pathogen occurrence in products from dairy cows. [ABSTRACT FROM AUTHOR]

Here, we describe a novel method using microwave ablation (MWA) guided by electromagnetic navigation bronchoscopy (ENB) and video‐assisted thoracoscopic surgery (VATS) for simultaneous treatment of multiple high‐risk pulmonary nodules in a 47‐year‐old woman. After the ENB registration process, the operator delivered the locatable electromagnetic probe to the target in the right upper lobe following the navigational route. MWA was performed after an antenna was passed into the lesion through the working channel. The wedge resection of the left upper lobe and lower lobe and the lingual segment resection were performed by VATS. The pathological diagnoses was adenocarcinoma in situ (AIS) of the right upper lobe lesion, AIS of the left upper lobe, minimally invasive adenocarcinoma of the left lower lobe lesion and chronic inflammation of the lingular segment. MWA guided by ENB combined with VATS is an alternative treatment strategy to deal with multiple pulmonary nodules at the same stage of the operation. [ABSTRACT FROM AUTHOR]

Maize (Zea mays L.) is one of the most important crops worldwide and is a model organism among cereal crops. Abiotic and biotic stresses are often present simultaneously and severely influence maize production, causing great yield losses worldwide. Therefore, the selection and cultivation of stress‐tolerant maize lines that adapt to various stresses is instrumental in addressing the problem of yield losses caused by stress. The maize mesocotyl is the crucial organ that pushes shoots out of deep water or soil after seed germination. It has a simple anatomy and exhibits rapid growth in the dark. In this article, we reviewed the studies on the elongation of the maize mesocotyl and the actions of phytohormones, especially under deep‐sowing conditions, and emphasized the role of the maize mesocotyl in response to environmental stress and deep‐sowing tolerance. We propose that the maize mesocotyl can serve as a selection organ for evaluating stress tolerance at the early seedling stage. We also identify future research fields that need further investigation in studies of the maize mesocotyl. [ABSTRACT FROM AUTHOR]

Due to the shielding structures of electronic system and coaxial lines feeding the antennas are complex, full‐wave algorithms applied for the coupling analysis of multiple linear antennas on electronic system excited by ambient wave should occupy amount of memories by meshing the structures of coaxial lines and electronic system in fine grid, which may affect the computation efficiency. This paper presents a time domain hybrid method, which consisting of thin wire finite‐difference time domain (FDTD) method, transmission line (TL) equations, higher order FDTD scheme, and an auto mesh generator technique. In this method, the thin wire FDTD combined with the auto mesh generator technique is utilized to mesh the shielding structure of electronic system rapidly and obtain the current responses on the conductor columns of linear antennas, which are extracted equivalent current sources for the further coupling analysis of coaxial lines. And then the TL equations are adopted to build the coupling models of coaxial lines, and solved by the higher order FDTD scheme to compute the transient responses on the coaxial lines and terminal loads of the lines. This method has one significant feature is that it can avoid modeling the structures of linear antennas and coaxial lines directly. The accuracy of this method has been verified by comparing with the simulation software Computer Simulation Technology (CST) via the coupling simulations of one and three linear antennas on electronic system excited by ambient plane wave. [ABSTRACT FROM AUTHOR]

Starch is the major form of carbohydrate storage in plants and exists as discrete starch granules (SGs). Isolation of high‐quality SGs in different plant tissues is a prerequisite for studying the roles of SGs during plant growth, development, and responses to abiotic stress. However, it is difficult to isolate transitory SGs from leaves and storage SGs from pollen grains due to their small sizes and low quantities. Herein, we develop a novel method for isolating SGs by using the aqueous two‐phase system (ATS) of ethanol/NaH2PO4. The ATS method efficiently separated SGs from contaminants based on their differences in density, solubility, and polarity. Using this method, we first isolated and purified three kinds of SGs from maize seeds, pollen, and leaves. The biochemical, microscopic, and proteomic analyses demonstrated the high purity of the isolated SGs. Proteomic analysis revealed distinct differences in SG‐bound proteins between seed SGs and pollen SGs. As a simple, rapid, and low‐cost method, the ATS‐based method exhibits highly universal and reproducible results for starch‐containing tissues in various plant species. We develop a novel and universal method for isolating starch granules (SGs) by use of an aqueous two‐phase system. The method allows to isolate even small quantities of transitory SGs in leaves and storage SGs in pollen. [ABSTRACT FROM AUTHOR]

Background: Aberrant methylation is a known cause of cancer initiation and/or progression. There are scant data on the genome-wide methylation pattern of nonfunctioning pancreatic neuroendocrine tumors (NFPanNETs) and sporadic and hereditary NFPanNETs.Methods: Thirty-three tissue samples were analyzed: they included samples from sporadic (n = 9), von Hippel-Lindau (VHL)-related (n = 10), and multiple endocrine neoplasia type 1 (MEN1)-related NFPanNETs (n = 10) as well as normal islet cells (n = 4) for comparison. Genome-wide CpG methylation profiling was performed with the Infinium MethylationEPIC BeadChip assay and was analyzed with R-based tools.Results: In unsupervised hierarchical clustering, sporadic and MEN1-related NFPanNETs clustered together, and the VHL group was in a separate cluster. MEN1-related NFPanNETs had a higher rate of hypermethylated CpG sites in comparison with sporadic and VHL-related tumor groups. Differentially methylated region analysis confirmed the higher rate of hypermethylation in MEN1-related tumors. Moreover, in an integrated analysis of gene expression data for the same tumor samples, downregulated gene expression was found in most genes that were hypermethylated. In a CpG island methylator phenotype analysis, 3 genes were identified and confirmed to have downregulated gene expression: secreted frizzle-related protein 5 (SFRP5) in sporadic NFPanNETs and cell division cycle-associated 7-like (CDCA7L) and RNA binding motif 47 (RBM47) in MEN1-related NFPanNETs.Conclusions: MEN1 NFPanNETs have a higher rate of geno me-wide hypermethylation than other NFPanNET subtypes. The similarity between the pathways enriched in a methylation analysis of known genes involved in NFPanNET tumorigenesis suggests a key role for aberrant methylation in the pathogenesis of NFPanNETs. [ABSTRACT FROM AUTHOR]

Using 15‐year observations obtained from the Moderate Resolution Imaging Spectroradiometer (MODIS) on board both NASA Terra and Aqua from March 2003 to February 2018, this study investigated the spatio‐temporal variations of both macro‐ and micro‐physical cloud properties over China, including cloud fraction (CF), cloud top pressure (CTP), cloud top temperature (CTT), cloud optical thickness (COT), and effective radius (re) of both liquid water and ice clouds. Multi‐year averaged CF is around 61% over whole China region. However, CF varies with both regions and seasons. The CFs are about 6–8% larger in summer and winter (~64–65%) than in spring and autumn (~58%). By classifying China into five regimes, which are northwestern China (NW), northeastern China (NE), Tibetan Plateau (TP), northern China (N), and southern China (S), there is a clear CF regional distribution pattern. In general, there are large amount of clouds in S and southeast of TP, and small amount in NE, N, NW and most TP. Moreover, there are generally more clouds over ocean than over land, and much more clouds over S than over N. The CFs are larger (smaller) in the afternoon than in the morning over most land (ocean) regions. Furthermore, the largest CF differences between afternoon and morning occur over the TP region in China. COT demonstrates almost the same regional distribution pattern as the CF for all four seasons. Specifically, COT is higher in S than in N, which is most likely associated with the type of clouds and the availability of water vapour. Cloud re shows larger values in NW and TP than in eastern China regions in all seasons except for summer, which could be related to the heavy aerosol pollution in eastern China regions. Accompanying with the cold cloud tops over TP, a low CTP centre is often located there. Using 15‐year MODIS observations, this study statistically analyses the cloud characteristics over China along with five regions of N, S, NW, NE, and TP. It is found that multi‐year averaged CF is ~61% over China, with ~64–65% in summer and winter, and ~58% in spring and autumn; and for most seasons, CF and COT are larger in S than N, CTT and CTP are lowest over TP, cloud re is larger in NW and TP than other regions. [ABSTRACT FROM AUTHOR]

Abstract: Exploring efficient and cheap oxygen evolution reaction (OER) electrocatalysts is of great significance for electrochemical water splitting. Herein, we successfully prepared an efficient ternary electrocatalyst of porous cobalt oxynitride (Co3OxNy) nanosheets by a simple nitridation strategy. Specifically, CoON PNS‐400 (cobalt oxynitride with porous nanosheet structure obtained at 400 oC) offered a low OER overpotential of 0.23 V to achieve the catalytic current density of 10 mA cm−2 and a small Tafel slope of 48 mV dec−1 in alkali media, outperforming most of the first‐row transition‐metal‐based OER electrocatalysts. The calculated density of states (DOS) analysis and electron spin resonance (ESR) measurements revealed that the introduction of foreign N atoms into pristine Co3O4 nanosheets can optimize the electronic structure and create more oxygen vacancies, thus leading to enhanced electrical conductivity. Density functional theory (DFT) calculations demonstrated that the foreign N atoms can also improve the energetics for OER by modulating the free energy for adsorbed intermediates (OOH*, OH*, O*), further improving the OER electrocatalytic activity of CoON PNS‐400. This work provides a possibility for rationally designing ternary transition‐metal compounds as advanced OER electrocatalysts. [ABSTRACT FROM AUTHOR]

Abstract: Rapid technological advances enabling the construction of designer gene networks, biosynthetic pathways, and even entire genomes are moving the fields of genetics and genomics from descriptive to synthetic applications. Following the synthesis of small viral genomes, advances in DNA assembly and rewriting have enabled the hierarchical synthesis of bacterial genomes, such as Mycoplasma genitalium, as well as recoding of the Escherichia coli genome by reducing the number of codons from 64 to 57. The field has advanced to the point of synthesizing an entire eukaryotic genome. The Synthetic Yeast Genome Project (Sc2.0) is underway and aims to rewrite all 16 Saccharomyces cerevisiae chromosomes by 2018; to date, 6.5 chromosomes have been designed and synthesized. Using bottom‐up assembly and applying genome‐wide alterations will improve our understanding of genome structure and function. This approach will not only provide a platform for systematic studies of eukaryotic chromosomes but will also generate diverse “streamlined” strains that are potentially suitable for medical and industrial applications. Herein, we review the current state of synthetic genome research and discuss potential applications of this emerging technology. [ABSTRACT FROM AUTHOR]

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The National Institutes of Health Clinical Center (NIH CC) is the largest hospital in the United States devoted entirely to clinical research, with a highly diverse spectrum of patients. Patient safety and clinical quality are major goals of the hospital, and therapy is often complicated by multiple cotherapies and comorbidities. To this end, we implemented a pharmacogenomics program in 2 phases. In the first phase, we implemented genotyping for HLA-A and HLA-B gene variations with clinical decision support (CDS) for abacavir, carbamazepine, and allopurinol. In the second phase, we implemented genotyping for drug-metabolizing enzymes and transporters: SLCO1B1 for CDS of simvastatin and TPMT for CDS of mercaptopurine, azathioprine, and thioguanine. The purpose of this review is to describe the implementation process, which involves clinical, laboratory, informatics, and policy decisions pertinent to the NIH CC. [ABSTRACT FROM AUTHOR]

Finding efficient electrocatalysts for oxygen evolution reaction (OER) that can be effectively integrated with semiconductors is significantly challenging for solar-driven photo-electrochemical (PEC) water splitting. Herein, amorphous cobalt-iron hydroxide (CoFeH) nanosheets are synthesized by facile electrodeposition as an efficient catalyst for both electrochemical and PEC water oxidation. As a result of the high electrochemically active surface area and the amorphous nature, the optimized amorphous CoFeH nanosheets exhibit superior OER catalytic activity in alkaline environment with a small overpotential (280 mV) to achieve significant oxygen evolution ( j = 10 mA cm−2) and a low Tafel slope (28 mV dec−1). Furthermore, CoFeH nanosheets are simply integrated with BiVO4 semiconductor to construct CoFeH/BiVO4 photoanodes that exhibit a significantly enhanced photocurrent density of 2.48 mA cm−2 (at 1.23 V vs reversible hydrogen electrode (RHE)) and a much lower onset potential of 0.23 V (vs RHE) for PEC-OER. Careful electrochemical and optical studies reveal that the improved OER kinetics and high-quality interface at the CoFeH/BiVO4 junction, as well as the excellent optical transparency of CoFeH nanosheets, contribute to the high PEC performance. This study establishes amorphous CoFeH nanosheets as a highly competitive candidate for electrochemical and PEC water oxidation and provides general guidelines for designing efficient PEC systems. [ABSTRACT FROM AUTHOR]

Single-phase, c-axis-oriented BiCuSeO thin films have been directly grown on the commercial silicon (001) wafers without any surface pretreatment by using pulsed laser deposition. Xray diffraction pole figure confirms that the film does not have any ab-plane texture, whereas cross-sectional transmission electron microscopy shows good crystallinity of the film even if there exists an amorphous native oxide layer on the wafers surface. At room temperature, the resistivity of the film is about 14 mΩ cm, which is much lower than that reported for corresponding single crystals as well as polycrystalline bulks. This work demonstrates the possibility of using the available state-of-the-art silicon processing techniques to create BiCuSeObased thin-film thermoelectric devices. [ABSTRACT FROM AUTHOR]

Mutations of vacuolar protein sorting-associated protein 33b (VPS33B) cause arthrogryposis, renal dysfunction, and cholestasis syndrome, and a lack of platelet 𝛂-granules in the affected patients. Conditional Vps33b knockout mice were developed to investigate the function(s) of Vps33b in platelet 𝛂-granule formation. We found that early embryonic deletion of Vps33b was lethal. PF4-Cre-driven megakaryocyte-targeted Vps33b gene deletion greatly diminished Vps33b expression in platelets, but had no effect on platelet 𝛂-granule formation and protein content. Tamoxifen-induced, haematopoietic stem cell (HSC)-specific Vps33b deletion completely depleted Vps33b in platelets, caused the absence of 𝛂-granules, and increased the number of vacuoles in platelets and megakaryocytes. VPS33B association with VIPAS39, 𝛂-tubulin, and SEC22B was identified by co-immunoprecipitation, mass spectra, and immunoblotting in human embryonic kidney 293T (HEK293T) cells. Also, pull-down experiments revealed that VIPAS39 bound to intact VPS33B; in contrast, 𝛂-tubulin and SEC22B separately interacted with the sec1-like domains of VPS33B. Vps33b deficiency in megakaryocytes disturbs the redistribution of Vipas39 and Sec22b to proplatelets, and interrupted the co-localization of Sec22b with Vwf-positive vesicles. The data presented in this study suggest that Vps33b is involved in 𝛂-granule formation possibly by facilitating the Vwf-positive vesicular trafficking to 𝛂-granule-related vacuoles in megakaryocytes. [ABSTRACT FROM AUTHOR]

In this paper, a novel, simple, but efficient method is reported to lower pressure drop and meanwhile maintain high filtration efficiency of superoleophobic filters for oil aerosol filtration. By close attachment of a thin oil‐guiding fibrous layer to the front surface of a superoleophobic filter, the pressure drop at pseudostatic state can be reduced by 20%. Such a remarkable reduction in filtration resistance is originated from the capillary effect of the oil‐guiding fabric, and pore size within the guiding fabric also played a role. This novel material may be useful for the development of high efficiency, low energy‐consumption coalescing filters for various applications. [ABSTRACT FROM AUTHOR]

Background Although multiple signaling cascades and molecules contributing to the pathophysiological process have been studied, the treatments for stroke against present targets have not acquired significant clinical progress. Although CARD3 (caspase activation and recruitment domain 3) protein is an important factor involved in regulating immunity, inflammation, lipid metabolism, and apoptosis, its role in cerebral stroke is currently unknown. Methods and Results Using a mouse model of ischemia-reperfusion (I-R) injury based on transient blockage of the middle cerebral artery, we have found that CARD3 expression is upregulated in a time-dependent manner during I-R injury. Further animal study revealed that, relative to control mice, CARD3-knockout mice exhibited decreased inflammatory response and neuronal apoptosis, with reduced infarct volume and lower neuropathological scores. In contrast, neuron-specific CARD3-overexpressing transgenic (CARD3-TG) mice exhibited increased I-R induced injury compared with controls. Mechanistically, we also found that the activation of TAK1 (transforming growth factor-β-activated kinase 1) was enhanced in CARD3-TG mice. Furthermore, the increased inflammation and apoptosis seen in injured CARD3-TG brains were reversed by intravenous administration of the TAK1 inhibitor 5Z-7-oxozeaenol. Conclusions These results indicate that CARD3 promotes I-R injury via activation of TAK1, which not only reveals a novel regulatory axis of I-R induced brain injury but also provides a new potential therapeutic approach for I-R injury.