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A critical measure of the quality of pharmaceutical proteins is the preservation of native conformations of the active pharmaceutical ingredients. Denaturation of the active proteins in any step before administration into patients could lead to loss of potency and/or aggregation, which is associated with an increased risk of immunogenicity of the products. Interfacial stress enhances protein instability as their adsorption to the air‐liquid and liquid–solid interfaces are implicated in the formation of denatured proteins and aggregates. While excipients in protein formulations have been employed to reduce the risk of aggregation, the roles of albumin as a stabilizer have not been reviewed from practical and theoretical standpoints. The amphiphilic nature of albumin makes it accumulate at the interfaces. In this review, we aim to bridge the knowledge gap between interfacial instability and the influence of albumin as a surface‐active excipient in the context of reducing the immunogenicity risk of protein formulations. [ABSTRACT FROM AUTHOR]

Antarctica has an active subglacial hydrological system, with interconnected subglacial lakes fed by subglacial meltwater. Subglacial hydrology can influence basal sliding, inject freshwater into the sub‐ice‐shelf cavity, and impact sediment transport and deposition which can affect the stability of grounding lines (GLs). We used satellite altimetry data from the ICESat, ICESat‐2, and CryoSat‐2 missions to document the second recorded drainage of Engelhardt Subglacial Lake (SLE), which began in July 2021 and discharged more than 2.3 km3 of subglacial water into the Ross Ice Shelf cavity. We used differential synthetic aperture radar interferometry from RADARSAT‐2 and TerraSAR‐X alongside ICESat‐2 repeat‐track laser altimetry (RTLA) and REMA digital elevation model strips to detect 2–13 km of GL retreat since the previous drainage event in 2003–06. Combining these satellite observations, we evaluated the mechanism triggering SLE drainage, the cause of the observed GL retreat, and the interplay between subglacial hydrology and GL dynamics. We find that: (a) SLE drainage was initiated by influx from a newly identified upstream lake; (b) the observed GL retreat is mainly driven by the continued retreat of Engelhardt Ice Ridge and long‐term dynamic thinning that caused a grounded ice plain to reach flotation; and (c) SLE drainage and GL retreat were largely independent. We also discuss the possible origins and influence of a 27 km grounded promontory found to protrude seaward from the GL. Our observations demonstrate the importance of high‐resolution satellite data for improving the process‐based understanding of dynamic and complex regions around the Antarctic Ice Sheet margins. Plain Language Summary: Large volumes of water flow beneath the Antarctic Ice Sheet through an interconnected network of rivers and lakes. This water system impacts slipperiness at the base of the ice, affecting how fast it moves. It also delivers freshwater into the ocean, directly contributing to sea‐level rise and increasing melt beneath the floating ice shelves. In this study, we use satellite data to track the 2021–24 drainage of Engelhardt Subglacial Lake in West Antarctica. This lake is located close to the Ross Ice Shelf grounding line, the point at the edge of the ice sheet where the ice first lifts off the bedrock and starts to float on the ocean. This region of the grounding line has retreated by up to 13 km since the last lake drainage in 2003–06. Here, we investigate what caused the drainage, the reasons for the grounding line retreat, and whether the two processes are connected. We also report the growth of a grounded promontory extending 27 km out to sea, which may be evidence of a former ice stream moraine or melt channel. These findings help to improve our limited understanding of the relationship between subglacial hydrology and grounding line dynamics in Antarctica. Key Points: Satellite altimetry detects second recorded drainage of Engelhardt Subglacial Lake in July 2021, triggered by an influx from an upstream lakeThe grounding line retreated by 2–13 km since the last drainage in 2003, linked to the retreat of Engelhardt Ice Ridge and ice plain ungroundingSatellite observations suggest that the 2021–24 lake drainage and grounding line retreat were largely independent processes [ABSTRACT FROM AUTHOR]

Many faculty members post accomplishments on faculty webpages, laboratory websites, or social media. Academia, career, interview, job talk, research, tenure Keywords: academia; career; interview; job talk; research; tenure EN academia career interview job talk research tenure 2393 2396 4 08/08/23 20230901 NES 230901 INTRODUCTION The objective of this two-part I CNSNT i editorial is to provide real-world, frank advice for scientists joining an academic or research institution. PRACTICE YOUR JOB TALK WITH CRITICAL THINKERS You have applied to an institution that is appropriately scaled for your type of work and you were successful at landing an interview request. [Extracted from the article]

Source‐to‐sink studies typically utilize the sedimentary record preserved in basins to infer source area parameters such as estimates of drainage area and discharges of fluvial systems in the hinterland, the characterization of which remains elusive as few geomorphic elements of the hinterland are preserved. Clastic wedges within foreland basin settings often contain fluvial deposits, and the scale of fluvial architectural elements can be used to estimate drainage area and discharge within the accreted hinterland. This study uses thicknesses of fluvial architectural elements within the Late Ordovician Taconic, the Late Devonian‐Early Mississippian Acadian, and the Late Mississippian to Early and Middle Pennsylvanian Alleghanian clastic wedges, together with climatic conditions interpreted from palaeosol data, to estimate the drainage areas and discharges of the river systems which drained the hinterlands of the accreted Taconic and Acadian terrains and the Alleghanian suture of Laurentia and Gondwana. Published detrital zircon age spectra provide insight into ages of source areas of sediments as well as the timing of terrane accretion. The increase in hinterland drainage areas calculated for Late Devonian Acadian Orogeny river systems compared to Late Ordovician Taconic Orogeny river systems can be attributed to the increase in size of accreted terranes to the east of the foreland basins during that period of time. The decrease in hinterland drainage area from the Late Devonian Acadian Orogeny to the Middle to Late Pennsylvanian Alleghanian Orogeny is attributed to the westward migration of the drainage divide as the Alleghanian orogeny proceeded. It is inferred that the drainage divide migrated towards the drier leeward side of the mountain range and caused drainage basins to shrink and split into smaller drainage basins. [ABSTRACT FROM AUTHOR]

In this work, we have proposed an electrochemical sensor for the detection of pesticides by using a ceramic composite with a SiO2 surface modified with Sm2O3 nanoparticles and C‐graphite (SSMG), obtained by the sol‐gel process and immobilized by adsorption in Meldola Blue cationic dye (MB). The composite was called (SSMG/MB), which was characterized by spectroscopic, electrochemical techniques, Fourier‐transform infrared spectroscopy, and Cyclic Voltammetry. The proposed sensor was applied for the glyphosate electrochemical detection, using Differential Pulse Voltammetry, and, under optimized parameters has presented the linear response for the pesticide in the concentration range from 0.99 to 7.94 (μmol L−1; R2=0.9963; n=8). The calculated values for the detection limit and the quantification limit were 0.15 and 0.49 μmol L−1, respectively. Therefore, the new electrochemical sensor based on SiO2, NPsSm2O3, C‐graphite, and MB hybrid material was developed for the first time for glyphosate determination, which has demonstrated high potential for the development of new hybrid devices for environmental control. [ABSTRACT FROM AUTHOR]

Academia, career, interview, laboratory, research, tenure Serving on grant proposal study sections, editorial boards, dissertation/theses committees, and society/conference committees can sharpen your research capacities, mentoring skills, and scientific reputation. Keywords: academia; career; interview; laboratory; research; tenure EN academia career interview laboratory research tenure 3118 3120 3 10/19/23 20231101 NES 231101 INTRODUCTION The objective of Part II of this I CNSNT i editorial series is to help you hit the ground running upon arrival at your new position. [Extracted from the article]

The InSight lander rests on a regolith‐covered, Hesperian to Early Amazonian lava plain in Elysium Planitia within a ∼27‐m‐diameter, degraded impact crater called Homestead hollow. The km to cm‐scale stratigraphy beneath the lander is relevant to the mission's geophysical investigations. Geologic mapping and crater statistics indicate that ∼170 m of mostly Hesperian to Early Amazonian basaltic lavas are underlain by Noachian to Early Hesperian (∼3.6 Ga) materials of possible sedimentary origin. Up to ∼140 m of this volcanic resurfacing occurred in the Early Amazonian at 1.7 Ga, accounting for removal of craters ≤700 m in diameter. Seismic data however, suggest a clastic horizon that interrupts the volcanic sequence between depths of ∼30 and ∼75 m. Meter‐scale stratigraphy beneath the lander is constrained by local and regional regolith thickness estimates that indicate up to 10–30 m of coarse‐grained, brecciated regolith that fines upwards to a ∼3 m thick loosely‐consolidated, sand‐dominated unit. The maximum depth of Homestead hollow, at ∼3 m, indicates that the crater is entirely embedded in regolith. The hollow is filled by sand‐size eolian sediments, with contributions from sand to cobble‐size slope debris, and sand to cobble‐size ejecta. Lander‐based observations indicate that the fill at Homestead hollow contains a cohesive layer down to ∼10–20 cm depth that is visible in lander rocket‐excavated pits and the HP3 mole hole. The surface of the landing site is capped by a ∼1 to 2 cm‐thick loosely granular, sand‐sized layer with a microns‐thick surficial dust horizon. Plain Language Summary: The InSight lander has geophysical instruments that are designed to determine the interior structure of Mars. Understanding the results from these instruments requires a geological analysis of materials beneath the landing site at Elysium Planitia. This study presents data that describe the vertical sequence of rocks and soils beneath the lander, as well as the geologic history. The results indicate that InSight rests on a 1.7‐billion‐year‐old lava plain that is covered in a 10–30 m thick regolith that was produced by impact cratering and modified by wind. The uppermost portion of the regolith is a ∼3 m thick horizon of sand. InSight rests on sand within a degraded impact crater. The sandy material contains a slightly cohesive horizon that is only ∼1–2 cm beneath the lander and is up to 10–20 cm thick. The sandy horizon overlies rock fragments that get progressively larger with depth. Bedrock of basaltic lava exists beneath the regolith down to a depth of ∼170 m. The bedrock is interrupted by weaker materials between depths of ∼30 and 75 m. Beneath ∼170 m, the sequence is dominated by ancient (3.7–4.1 billion years old), possibly sedimentary materials. Key Points: InSight rests on Early Amazonian basaltic lava with an up to 10–30 m thick regolith. The upper 3 m of the regolith is sand dominatedThe regolith contains a 10–20 cm thick cohesive horizon or duricrust. This horizon rests 1–2 cm beneath the landerThe upper 1–2 cm of the regolith comprises loosely‐consolidated sand to pebbles. Sand is rarely mobilized under current wind conditions [ABSTRACT FROM AUTHOR]

Rock shapes and heights around the InSight lander are examined to refine the degradation history of the 27 m‐diameter Homestead hollow. Results document decreasing average exposed rock height and increasing percentage of rocks where height comprises the short axis from outside to within the hollow and support prior models of ejecta deflation accompanied by hollow infilling. We estimate 0.3 m of deflation at the current rim that is realistic compared to rock relief, original ejecta thickness, and predicted aeolian contributions to infilling. We also find that shapes of embayed basalt rocks outside the hollow appear platy, bladed, and elongate in a triangular form factor plot, and more discoidal and bladed in an axes ratio plot. By contrast, expected shapes based on terrestrial studies of basalt rocks are mostly compact, compact platy, compact bladed, compact elongated, platy, bladed, and elongate in triangular form factor plots, and equant with lesser, but significant disc‐ and blade‐shaped rocks in axes ratio plots. We find addition of 10 cm to the heights of rocks near the hollow rim, to account for continued partial embedding in ejecta, yields the best match between observed and expected rock shapes. Exposure of small ejecta rocks in the hollow supports degradation rates of 10−4 m/Myr during most of hollow history. Results indicate that deflation from ejecta accompanied by downwind deposition in the hollow can account for the current degraded form of the crater. Our approach is a new tool for characterizing small crater degradation on regolith‐covered lava plains on Mars. Key Points: Rock heights and shapes at Homestead hollow indicate early transport of deflated ejecta sediments can account for infilling of the craterNear‐rim rock heights and shapes relative to the expected original versus remnant ejecta thickness indicates 30 cm deflation at the current rimExposure of later arriving, small ejecta rocks in the hollow supports very low estimates of degradation over most hollow history [ABSTRACT FROM AUTHOR]

Geologic and climatic processes on modern‐day Mars are heavily influenced by aeolian surface activity, yet the relationship between atmospheric conditions and sediment mobilization is not well understood. The Interior Exploration using Seismic Investigations, Geodesy, and Heat Transport (InSight) spacecraft is uniquely able to address this issue, due to its joint imaging and continuous high‐frequency meteorological capabilities, which allow for direct comparison between surface activity and atmospheric conditions. Since landing in the volcanic plains of Elysium Planitia, InSight's camera's have recorded intermittent, small‐scale surface changes, including removal of fine material on the lander footpad, linear tracks and localized surface darkening caused by minor dust removal, and surface creep of granules, as presented in Part 1 (Charalambous et al., 2021, this issue). Surface activity is found to correlate well with the timing of abrupt pressure drops (ΔP ∼ 1–9 Pa) and transient wind gusts (v ∼ 14–31 m/s) associated with convective vortex passage. Here we identify the major erosive forces acting on surface particles during these events, including the vertical pressure gradient force at the vortex core and the drag force generated by quickly‐rotating tangential winds. Orbital and ground‐truth data suggest that aeolian activity at InSight's landing site is sporadic under modern climatic conditions. Ongoing aeolian surface modifcation is driven primarily by turbulent vortices that sporadically lift dust and redistribute coarser sediment (i.e., sand and granules) but do not aid in the development of organized aeolian bedforms. Surface erosion is localized within the path these vortices take across the surface which is controlled by seasonally‐reversing background circulation patterns. Plain Language Summary: Numerous orbiting and landed spacecraft have imaged wind‐driven ("aeolian") motion of sand and dust on Mars. However, spacecraft on the surface have generally lacked the instrumentation required to interpret the atmospheric conditions driving this motion. Here we utilize the unique opportunity provided by the NASA InSight lander's joint imaging and meteorological capabilities to closely interpret ongoing aeolian activity at the spacecraft's landing site at Elysium Planitia and similar plains regions across Mars. During the first 400 martian days (sols) of the mission, intermittent, small‐scale motion of dust and sand are observed in association with local, thermally driven vortices passing over or near the lander. In situ data are also compared to orbital images and atmospheric model predictions to obtain a more comprehensive picture of wind‐related processes across the region. Overall, aeolian surface modification at this location is found to be very limited under current climatic conditions and is likely dominated by redistribution of fine material caused by transient convective events. Key Points: NASA's InSight mission provides a chance to study aeolian transport through concurrent imaging and high‐frequency atmospheric monitoringThe surface at InSight's landing site is generally stable besides intermittent surface activity caused by convective vortex passageThe vertical pressure gradient within the vortex core and increased drag caused by rotational winds help facilitate surface erosion [ABSTRACT FROM AUTHOR]

Metabolic heart disease (MHD), which is strongly associated with heart failure with preserved ejection fraction, is characterized by reduced mitochondrial energy production and contractile performance. In this study, we tested the hypothesis that an acute increase in ATP synthesis, via short chain fatty acid (butyrate) perfusion, restores contractile function in MHD. Isolated hearts of mice with MHD due to consumption of a high fat high sucrose (HFHS) diet or on a control diet (CD) for 4 months were studied using 31P NMR spectroscopy to measure high energy phosphates and ATP synthesis rates during increased work demand. At baseline, HFHS hearts had increased ADP and decreased free energy of ATP hydrolysis (ΔG~ATP), although contractile function was similar between the two groups. At high work demand, the ATP synthesis rate in HFHS hearts was reduced by over 50%. Unlike CD hearts, HFHS hearts did not increase contractile function at high work demand, indicating a lack of contractile reserve. However, acutely supplementing HFHS hearts with 4mM butyrate normalized ATP synthesis, ADP, ΔG~ATP and contractile reserve. Thus, acute reversal of depressed mitochondrial ATP production improves contractile dysfunction in MHD. These findings suggest that energy starvation may be a reversible cause of myocardial dysfunction in MHD, and opens new therapeutic opportunities. [ABSTRACT FROM AUTHOR]

The InSight mission to Mars landed within Homestead hollow on an Early Amazonian lava plain. The hollow is a 27‐m‐diameter, 0.3‐m‐deep quasi‐circular depression that shares morphologic and sedimentologic characteristics to degraded impact craters. Unlike the intercrater plains outside of the hollow, the interior lacks cobbles and is dominated by loose sand, granules, and pebbles. Fresher craters near the landing site exhibit meter‐scale bedforms in their ejecta and on their floors due to sediment trapping. The sedimentology of the interior fill of Homestead hollow suggests similar trapping. The hollow falls along a morphologic continuum that requires low rates of rim degradation and fill. Crater degradation rates (rim erosion plus filling) in the landing site decline nonlinearly through time from 10−2 to 10−4 m/Myr as craters evolve to a hollow‐like form. Rim erosion rates are lower initially, at 10−3 m/Myr, but converge with degradation rates to 10−4 m/Myr. This implies that while filling plays an important role soon after crater formation, it is limited in later stages. Crater statistics indicate that the bulk of the fill occurred in the first ~50 Myr for Homestead hollow. The estimated maximum age of the hollow is ~400 to 700 Myr. This requires near‐zero fill aggradation and long‐term soil stability for the bulk of the crater's history. Fill stability manifests in Homestead hollow as a ~5‐ to 10‐cm‐thick duricrust, formed by exchanges of atmospheric water vapor with soil. The estimated degradation in the hollow requires ~2 to 3 m of sedimentary fill beneath the lander. Plain Language Summary: The Interior Exploration using Seismic Investigation, Geodesy and Heat Transport (InSight) mission to Mars landed in western Elysium Planitia. The landing site consists of a smooth, flat surface that is unique relative to the surrounding rockier plains. Observations from orbital satellite imagery indicate that InSight landed inside of an almost circular depression in the landscape. Here we evaluate the shape and form of this small circular depression and compare it against nearby degraded impact craters. We conclude that InSight landed inside a very old crater that has filled in with windblown sediment and slope debris from the crater wall. We also estimate that the crater has been sitting on the landscape, experiencing extremely slow degradation, for approximately 400 to 700 Myr. The crater origin for the depression has implications for our understanding of the materials that exist immediately beneath the lander and the results from the two geophysical instruments onboard InSight, the Seismic Experiment for Interior Structure (SEIS) and Heat Flow and Physical Properties Package (HP3). Key Points: InSight landed within a 27‐m‐diameter, 0.3‐m‐deep, degraded impact crater called Homestead hollowHomestead hollow has a maximum age of 400 to 700 Myr. Degradation rates were two orders of magnitude higher in first 50 Myr after impactHomestead hollow is filled with up to 3 m of loose infill that is composed of sand and pebbles. The hollow fill is capped by a duricrust [ABSTRACT FROM AUTHOR]

Background: Childhood cancer is neglected within global health. Oxford Pediatrics Linking Oncology Research with Electives describes early outcomes following collaboration between low- and high-income paediatric surgery and oncology centres. The aim of this paper is twofold: to describe the development of a medical student-led research collaboration; and to report on the experience of Wilms' tumour (WT). Methods: This cross-sectional observational study is reported as per STROBE guidelines. Collaborating centres included three tertiary hospitals in Tanzania, Rwanda and the UK. Data were submitted by medical students following retrospective patient note review of 2 years using a standardised data collection tool. Primary outcome was survival (point of discharge/death). Results: There were 104 patients with WT reported across all centres over the study period (Tanzania n = 71, Rwanda n = 26, UK n = 7). Survival was higher in the high-income institution [87% in Tanzania, 92% in Rwanda, 100% in the UK (X2 36.19, p < 0.0001)]. Given the short-term follow-up and retrospective study design, this likely underestimates the true discrepancy. Age at presentation was comparable at the two African sites but lower in the UK (one-way ANOVA, F = 0.2997, p = 0.74). Disease was more advanced in Tanzania at presentation (84% stage III–IV cf. 60% and 57% in Rwanda and UK, respectively, X2 7.57, p = 0.02). All patients had pre-operative chemotherapy, and a majority had nephrectomy. Post-operative morbidity was higher in lower resourced settings (X2 33.72, p < 0.0001). Methodology involving medical students and junior doctors proved time- and cost-effective. This collaboration was a valuable learning experience for students about global research networks. Conclusions: This study demonstrates novel research methodology involving medical students collaborating across the global south and global north. The comparison of outcomes advocates, on an institutional level, for development in access to services and multidisciplinary treatment of WT. [ABSTRACT FROM AUTHOR]

Summary: Plant eradication is difficult, particularly in remote, protected areas. The Southern Ocean Islands are very isolated and highly protected, but the flora contains many alien plants. Small restricted populations have been eradicated, but eradication of established species has proven difficult. A better understanding of the efficacy of control methods at sub‐Antarctic temperatures and their off‐target impacts may increase eradication success. With interest in controlling non‐native Poa annua in the region, we aimed to determine if physical and chemical methods can control P. annua (the sub‐Antarctic biotype) in sub‐Antarctic conditions and examined their impact on native plants. We quantified the effectiveness of physical control methods on P. annua in situ on sub‐Antarctic Macquarie Island through field‐based experiments and assessed their selectivity on P. annua compared with native grasses. We also quantified the effectiveness of several herbicides on P. annua at sub‐Antarctic temperatures and assessed their selectivity on native grasses. Of the four physical disturbance methods tested, none effectively reduced P. annua cover as one‐off treatments. Of the herbicide treatments, glyphosate and trifloxysulfuron sodium were effective and were less damaging to native grass species, indicating potential selectivity. Physical control was of limited effectiveness, but did not affect native species richness. An integrated weed management programme utilising the strategic use of selective herbicides with follow‐up chemical and physical intervention may balance control and biodiversity outcomes. This research highlights the importance of site‐specific testing of control methods and understanding off‐target impacts of control when managing alien plant species in protected areas. [ABSTRACT FROM AUTHOR]

Exploration of Endeavour crater's Shoemaker formation rim rocks by the Opportunity rover, combined with extensive observations from the Mars Express and Mars Reconnaissance Orbiters, provides unique and quantitative insights into the processes that have degraded this 22‐km diameter Noachian age impact crater. These insights are informed by comparisons between Endeavour and the relatively young, and morphologically fresh appearing, 19‐km diameter Bopolu crater located 65 km to the southwest. Analyses of rover and orbiter data, combined with landscape evolution modeling using Bopolu topography as a starting point, demonstrate that significant weathering and fluvial degradation of Endeavour occurred during the Noachian Period, with ~0.3 km of vertical rim removal, ~0.9‐km backwasting of the rim, and deposition of ~0.5 km of fluvial‐deltaic‐lacustrine sediments on the crater floor, sourced from rim erosion. Pediments formed on external rim segments, with characteristic thin regolith covers over graded bedrock. Late Noachian to Early Hesperian age Grasberg formation draping sediments, and Burns formation sulfate‐rich sandstones, subsequently embayed all but high standing Shoemaker formation rocks. Burns formation strata accumulated in the crater interior up to a depth of ~0.8 km. Subsequent wind erosion of the interior deposits is indicated by an interior mound and moat, together with yardangs carved into Shoemaker formation rocks, and graded topographic profiles from Grasberg and Burns formation rocks up and through the interior rim Shoemaker formation outcrops. Up to ~0.4 km of Burns formation rocks were exhumed from within the crater by wind erosion to produce the mound and moat. Plain Language Summary: The Opportunity Rover explored the rim of the Noachian age Endeavour crater from 2010 to 2018. Only small segments of its rim are exposed above a regional sulfate‐bearing sandstone unit called the Burns formation. By studying the morphology of these rim segments with orbital data sets and observations from Opportunity, the history of the degradation of the crater was identified. The nearby Bopolu crater is similar in size to Endeavour but is younger and fresher. Simulating the erosion of Bopolu to create a crater rim that looks similar to Endeavour's current rim shows that Endeavour experienced a late Noachian period of fluvial erosion in an arid environment. Erosion lowered the crater rim by ~0.3 km, routing ~0.5‐km thick section of sediment into the crater interior, where a very shallow lake was likely present. Endeavour was then partially buried by Grasberg and Burns formation rocks. Since then, regional winds coming from the east have excavated some of the interior sandstone unit in Endeavour and carved into the interior rim, including Perseverance Valley. Key Points: Landscape evolution modeling indicates that Endeavour experienced an early period of fluvial erosion in a semiarid environmentSince the deposition of the Grasberg and Burns formation rocks, wind erosion has excavated interior deposits and eroded the crater rimUp to ~400 m of Burns formation rocks have been excavated from the interior of Endeavour crater [ABSTRACT FROM AUTHOR]

There has been an increase in the number of units providing anaesthesia for magnetic resonance imaging and the strength of magnetic resonance scanners, as well as the number of interventions and operations performed within the magnetic resonance environment. More devices and implants are now magnetic resonance imaging conditional, allowing scans to be undertaken in patients for whom this was previously not possible. There has also been a revision in terminology relating to magnetic resonance safety of devices. These guidelines have been put together by organisations who are involved in the pathways for patients needing magnetic resonance imaging. They reinforce the safety aspects of providing anaesthesia in the magnetic resonance environment, from the multidisciplinary decision making process, the seniority of anaesthetist accompanying the patient, to training in the recognition of hazards of anaesthesia in the magnetic resonance environment. For many anaesthetists this is an unfamiliar site to give anaesthesia, often in a remote site. Hospitals should develop and audit governance procedures to ensure that anaesthetists of all grades are competent to deliver anaesthesia safely in this area. [ABSTRACT FROM AUTHOR]

Abstract: Uzboi Vallis in northwestern Noachis Terra is the uppermost segment of the Noachian to Hesperian Uzboi‐Ladon‐Morava mesoscale outflow system that dominates regional northward drainage through Noachis and Margaritifer Terrae. Recently acquired High Resolution Imaging Science Experiment (HiRISE) and Compact Reconnaissance Imaging Spectrometer for Mars (CRISM) data, as well as topography, help to characterize the nature and origin of a previously unrecognized deposit on the floor of Uzboi Vallis at the confluence of Nirgal Vallis. The Uzboi floor deposit consists of a lower unit (~255 km3) and an upper unit (~34 km3). The origin and age of the lower unit are less constrained; it may have formed by a combination of impact (e.g., ejecta from Luki crater), fluvial/alluvial, and (or) mass wasting processes. The superimposed upper unit is symmetrical relative to the mouth of Nirgal, fan‐shaped in planform and layered. The morphology and location of the upper unit are consistent with alluvial (and possibly debris flow) deposition related to discharge from Nirgal Vallis. Crater Luki and the upper unit likely postdate the draining of the short‐lived lake in Uzboi that formed after the mid‐ to Late Hesperian Holden crater blocked the northern end of Uzboi. Late‐stage aqueous activity from Nirgal may have been sustained to some extent by groundwater discharge from aquifers along Nirgal that were recharged when Uzboi hosted a lake and (or) somewhat limited precipitation‐fed surface runoff. The associated climate in the mid‐ to Late Hesperian was favorable for such aqueous processes, and it may have provided habitable surface conditions in this region relatively late in Martian history. [ABSTRACT FROM AUTHOR]

Abstract: Aim: The aim of this study was to test the mediating role of the satisfaction of dental students' basic psychological needs of autonomy, competence and relatedness on the association between learning climate, feedback and student motivation. The latter was based on the self‐determination theory's concepts of differentiation of autonomous motivation, controlled motivation and amotivation. Methods: A cross‐sectional correlational study was conducted where 924 students completed self‐reported questionnaires measuring motivation, perception of the learning climate, feedback and basic psychological needs satisfaction. Descriptive statistics, Cronbach's alpha scores and bivariate correlations were computed. Mediation of basic needs on each predictor‐outcome association was tested based on a series of regression analyses. Finally, all variables were integrated into one structural equation model, controlling for the effects of age, gender and year of study. Results: Cronbach's alpha scores were acceptable (.655 to .905). Correlation analyses showed positive and significant associations between both an autonomy‐supportive learning climate and the quantity and quality of feedback received, and students' autonomous motivation, which decreased and became negative when correlated with controlled motivation and amotivation, respectively. Regression analyses revealed that these associations were indirect and mediated by how these predictors satisfied students' basic psychological needs. These results were corroborated by the structural equation analysis, in which data fit the model well and regression paths were in the expected direction. Conclusion: An autonomy‐supportive learning climate and the quantity and quality of feedback were positive predictors of students' autonomous motivation and negative predictors of amotivation. However, this was an indirect association mediated by the satisfaction of students' basic psychological needs. Consequently, supporting students' needs of autonomy, competence and relatedness might lead to optimal types of motivation, which has an important influence on dental education. [ABSTRACT FROM AUTHOR]

Abstract: Water relations in plant communities are influenced both by contrasting functional groups (grasses and shrubs) and by climate change via complex effects on interception, uptake, and transpiration. We modeled the effects of functional group replacement and biomass increase, both of which can be outcomes of invasion and vegetation management, and climate change on ecological drought (soil water potential below which photosynthesis stops) in 340 semiarid grassland sites over 30 year periods. Relative to control vegetation (climate and site‐determined mixes of functional groups), the frequency and duration of drought were increased by shrubs and decreased by annual grasses. The rankings of shrubs, control vegetation, and annual grasses in terms of drought effects were generally consistent in current and future climates, suggesting that current differences among functional groups on drought effects predict future differences. Climate change accompanied by experimentally increased biomass (i.e., the effects of invasions that increase community biomass or management that increases productivity through fertilization or respite from grazing) increased drought frequency and duration and advanced drought onset. Our results suggest that the replacement of perennial temperate semiarid grasslands by shrubs, or increased biomass, can increase ecological drought in both current and future climates. [ABSTRACT FROM AUTHOR]

Decision methods applied in the remediation industry were evaluated using a survey of industry practitioners, in order to assess the relative roles of quantitative decision analysis and gut intuition. Principals from the disciplines of behavioral economics and decision theory were used as a framework to evaluate remediation decision behaviors revealed by the survey. The survey was completed by 118 respondents representing academia, consultants, clients, and others. Survey questions focused on perceptions and experiences related to inputs to decisions and decision processes, as well as remediation goal setting and outcomes. The most common remediation objective cited was short-term interim measures and the least common was no further action (NFA) with clean closure. NFA was also sparingly achieved: 33% of respondents reported zero NFA closures in their career, and an overall 15% to 20% NFA closure rate was reported among more experienced respondents. Data inputs were ranked most important to decisions, while the decision process itself was ranked lowest. Intuition-based decision methods such as asking for a trusted opinion, rules of thumb, and meetings were all used at last twice as often as decision analysis such as discounted cash flow or probabilistic analysis. Analysis of survey responses showed that cognitive biases, including overconfidence effect and intuition bias, are present to some extent in remediation decision-making. Practitioners are advised to be mindful of the decision-making processes they apply, and to incorporate elements of both intuition and decision analysis, as appropriate to the decisions being made. [ABSTRACT FROM AUTHOR]

Objective: Metabolic syndrome (MetS) can lead to myocardial fibrosis, diastolic dysfunction, and eventual heart failure. This study evaluated alterations in myocardial microstructure in people with MetS by using a novel algorithm to characterize ultrasonic signal intensity variation.Methods: Among 254 participants without existing cardiovascular disease (mean age 42 ± 11 years, 75% women), there were 162 with MetS, 47 with obesity without MetS, and 45 nonobese controls. Standard echocardiography was performed, and a novel validated computational algorithm was used to investigate myocardial microstructure based on sonographic signal intensity and distribution. The signal intensity coefficient (SIC [left ventricular microstructure]) was examined.Results: The SIC was significantly higher in people with MetS compared with people with (P < 0.001) and without obesity (P = 0.04), even after adjustment for age, sex, body mass index, hypertension, diabetes mellitus, and the ratio of triglyceride (TG) to high-density lipoprotein (HDL) cholesterol (P < 0.05 for all). Clinical correlates of SIC included TG concentrations (r = 0.21, P = 0.0007) and the TG/HDL ratio (r = 0.2, P = 0.001).Conclusions: This study's findings suggest that preclinical MetS and dyslipidemia in particular are associated with altered myocardial signal intensity variation. Future studies are needed to determine whether the SIC may help detect subclinical diseases in people with metabolic disease, with the ultimate goal of targeting preventive efforts. [ABSTRACT FROM AUTHOR]

The pharyngeal jaw apparatus is a key innovation hypothesised to increase foraging efficiency and facilitate utilisation of novel resources among teleost fishes. Here, we tested whether dietary characteristics could predict pharyngeal jaw morphology among eight species of Neotropical Crenicichla. Additionally, we tested the hypothesis that pharyngeal jaws may impose a functional constraint on piscivory via pharyngeal gape. We quantified the shape of the lower pharyngeal jaw ( LPJ) using linear and geometric morphometrics and quantified diet using the relative volumetric proportions of prey items. We used principal component analysis to describe major axes of variation in LPJ shape and dietary patterns. The major axis of dietary variation significantly predicted LPJ morphology, which was driven by a significant relationship between LPJ shape and rates of piscivory. We also found that rates of piscivory predicted size-corrected LPJ depth. Size-corrected pharyngeal gape also significantly predicted rates of piscivory such that pharyngeal jaws may constrain piscivory by limiting pharyngeal gape. Strong form-to-function linkage between pharyngeal morphology and trophic patterns suggests an adaptive quality of the pharyngeal apparatus but may also impose functional constraints when consumers must switch prey or when prey availability is temporally or spatially unpredictable. [ABSTRACT FROM AUTHOR]

Hendra virus (HeV), a potentially fatal zoonotic disease spread by flying foxes, to date has always infected humans via a spillover event from equine HeV infection. In a theoretical case study, we compared the impacts of two different HeV prevention strategies - vaccination and flying fox roost removal - using a recently developed framework that considers different stakeholder group perspectives. The perspectives of the four selected stakeholder groups regarding intangibles were inferred from public discussions and coverage in the media. For all stakeholder groups, the option to vaccinate horses was found to add value to the economic results when the intangible impacts were included in the analysis, while the option for roost removal unanimously detracted from economic analysis value when the intangible impacts were included. Both the mean and median stakeholder-adjusted value ratios (2.25 and 2.12, respectively) for vaccination were inflated when intangible impacts were included, by value-adding to the results of a traditional economic analysis. In the roost removal strategy, these ratios (1.19 and 1.16, respectively) were deflated when intangible impacts were included. Results of this theoretical study suggest that the inclusion of intangible impacts promotes the value of a two-dose initial vaccination protocol using a subunit vaccination considered to offer complete protection for horses, as a strategy to control HeV, whereas roost removal becomes an even more costly strategy. Outcome of the analysis is particularly sensitive to the intangible value placed on human health. Further evaluation - via sociological methods - of values placed on intangibles by various stakeholder groups is warranted. [ABSTRACT FROM AUTHOR]

High numbers of eosinophils are observed in parasitic infections and allergic diseases, where they are proposed to be terminally differentiated effector cells that play beneficial role in host defence, or cause harmful inflammatory response. Eosinophils have been associated with killing of schistosomulae in vitro, but there is growing evidence that eosinophils can play additional immuno-regulatory role. Here, we report results of a study that examines peripheral blood mononuclear cell ( PBMC) cytokine responses to Schistosoma mansoni adult worm antigen ( SWA) when stimulated alone or enriched with autologous eosinophils. Production of the Th-2 type cytokines interleukin ( IL)-4, IL-5 and IL-13 was lower (P = 0·017, 0·018 and <0·001, respectively) in PBMC + eosinophil cultures than in PBMC-only cultures stimulated with SWA. Substantial levels of IL-13, IL-10, interferon gamma and tumour necrosis factor alpha were recorded in cultures of eosinophils, but none of these cytokines showed significant association with the observed eosinophil-induced drop in cytokine responses of PBMC. Transwell experiments suggested that the observed effect is due to soluble mediators that downmodulate production of Th-2 type cytokines. This study shows that eosinophils may down-modulate schistosome-specific Th-2 type cytokine responses in S. mansoni-infected individuals. The mechanism of this immune modulation remains to be elucidated. [ABSTRACT FROM AUTHOR]

We present a system of nonlinear ordinary differential equations used to quantify the complex dynamics of the interactions between tumor growth, vasculature generation, and antiangiogenic treatment. The primary dataset consists of longitudinal tumor size measurements (1,371 total observations) in 105 colorectal tumor-bearing mice. Mice received single or combination administration of sunitinib, an antiangiogenic agent, and/or irinotecan, a cytotoxic agent. Depending on the dataset, parameter estimation was performed either using a mixed-effect approach or by nonlinear least squares. Through a log-likelihood ratio test, we conclude that there is a potential synergistic interaction between sunitinib when administered in combination with irinotecan in preclinical settings. Model simulations were then compared to data from a follow-up preclinical experiment. We conclude that the model has predictive value in identifying the therapeutic window in which the timing between the administrations of these two drugs is most effective. [ABSTRACT FROM AUTHOR]

We propose confocal laser feedback microscopy for in‐depth imaging of highly scattering samples. This technique provides a compact configuration for microscopy in reflectance mode; it is based on laser feedback interferometry which is a sensing technique where the laser acts as both transmitter and receiver of the beam. Operating at 850 nm, it offers an ideal platform for non‐invasive and in vivo imaging of soft biological tissues. To explore the technique, the authors performed microscopic imaging of micro‐glass‐spheres (with diameter sizes of 10–20 µm) deep within an agar gel sample, at depth of 0.43 mm from the surface. Experimental results show the feasibility of a compact, low‐cost, and simple laser‐scanning microscope with possible biomedical imaging applications. [ABSTRACT FROM AUTHOR]

Demonstrating the effect that climate change is having on regional weather is a subject which occupies climate scientists, government policy makers and the media. After an extreme weather event occurs, the question is often posed, 'Was the event caused by anthropogenic climate change?' Recently, a new branch of climate science (known as attribution) has sought to quantify how much the risk of extreme events occurring has increased or decreased due to climate change. One method of attribution uses very large ensembles of climate models computed via volunteer distributed computing. A recent advancement is the ability to run both a global climate model and a higher resolution regional climate model on a volunteer's home computer. Such a set-up allows the simulation of weather on a scale that is of most use to studies of the attribution of extreme events. This article introduces a global climate model that has been developed to simulate the climatology of all major land regions with reasonable accuracy. This then provides the boundary conditions to a regional climate model (which uses the same formulation but at higher resolution) to ensure that it can produce realistic climate and weather over any region of choice. The development process is documented and a comparison to previous coupled climate models and atmosphere-only climate models is made. The system (known as weather@home) by which the global model is coupled to a regional climate model and run on volunteers' home computers is then detailed. Finally, a validation of the whole system is performed, with a particular emphasis on how accurately the distributions of daily mean temperature and daily mean precipitation are modelled in a particular application over Europe. This builds confidence in the applicability of the weather@home system for event attribution studies. [ABSTRACT FROM AUTHOR]

The characterisation of surface roughness is important in process control and metrology. A simple, low cost sensor which can be used for this measurement is one based on laser optical feedback interferometry. A target containing samples of similar abrasive paper with different levels of roughness was characterised using a sensor based on this technique, and the resulting measurements show that the standard deviation of the optical feedback signal was proportional to the roughness of the surface. [ABSTRACT FROM AUTHOR]

Objectives To study which salivary proteins form the protective bound mucosal pellicle and to determine the role of transglutaminase in pellicle development. Materials and Methods Oral epithelial cells were collected and underwent washes of different strengths, followed by homogenisation. SDS- PAGE, western blotting, IgA ELISAs and amylase activity assays were completed on cell homogenates and compared to saliva samples to confirm which salivary proteins were bound to cell surfaces. Results Salivary mucins, MUC5B and MUC7, were strongly retained on the oral epithelial cell surface. Other bound proteins including cystatin S, carbonic anhydrase VI, secretory component and IgA could be washed off. IgA was present in concentrated levels in the bound mucosal pellicle compared to amounts in saliva. Amylase, one of the most abundant proteins present in saliva, showed minimal levels of binding. Transglutaminase 3 presence was confirmed, but proteins that it catalyses cross-links between, statherin and proline-rich proteins, showed minimal presence. Conclusion Some protective salivary proteins including mucins and IgA become concentrated on oral surfaces in the bound mucosal pellicle, through specific interactions. Concentration of mucins would contribute to lubrication to prevent abrasion damage to soft tissues, whilst increased IgA could create an 'immune reservoir' against mucosal infection. [ABSTRACT FROM AUTHOR]

Psychological detachment from work is important for facilitating recovery. This can be threatened by rumination, or thinking about the day's stressors. Rumination may lead to distress, fatigue and extended activation of stress-related systems, but findings are not unequivocal. Level of construal (abstract or concrete) and type of mentation (imagery or verbal thought) used during stressor-focused rumination may shape physiological and affective responses and impact recovery. This study tested whether blood pressure (BP) and anxiety responses to stressor-focused rumination differ by mentation type and construal level. Healthy undergraduates ( n = 136) performed a speech stressor and then completed a rumination task in one of four randomly assigned conditions: concrete imagery, abstract imagery, concrete verbal thought or abstract verbal thought. Anxiety and continuous BP were assessed. Concrete rumination led to greater BP, whereas rumination with abstract construals led to lower BP. Furthermore, participants in the abstract conditions had greater increases in anxiety following stressor-focused rumination than in the concrete conditions. Results suggest that the immediate physiological and psychological consequences of stressor-focused rumination depend upon mode of thought. Copyright © 2014 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]

Left displacement of the abomasum (LDA) is an important periparturient disorder of dairy cows. This study evaluated differences in metabolic parameters between case-control pairs of cows (n=67) from 24 farms, and related these to outcomes in fertility and production. Cows with an assisted delivery were ×3 more likely to develop LDA, and affected cows tended to have had a longer dry period. At recruitment, cows with LDA tended to be in lower body condition accompanied by significantly higher circulating concentrations of β-hydroxybutyrate (BHB), non-esterified fatty acid (NEFA) and glucose and lower IGF1. Overall culling rate for all cows in the subsequent lactation was 22.5 per cent. Cows with LDA were not at increased odds of being culled but they produced, on average, 2272 l less milk and tended to have longer intervals to conception. Considering all cows irrespective of LDA status, the mean IGF1 level at recruitment was the only measured parameter associated with subsequent risk of culling (culled 11.7 ng/ml, not culled 23.5 ng/ml; P=0.005). Our findings support previous work indicating that poor insulin sensitivity through an uncoupling of the somatotrophic axis may be an important factor associated with LDA. Improved nutritional management of dry cows should reduce the incidence of both LDA and culling. [ABSTRACT FROM AUTHOR]

Background Crohn's disease is a debilitating chronic inflammatory bowel disease. Appropriate use of diet and nutritional therapy is integral to the overall management strategy of Crohn's disease. The aim was to develop evidence-based guidelines on the dietary management of Crohn's disease in adults. Methods Questions relating to the dietary management of Crohn's disease were developed. These included the roles of enteral nutrition to induce remission, food re-introduction diets to structure food re-introduction and maintain remission, and dietary management of stricturing disease, as well as whether probiotics or prebiotics induce or maintain remission. A comprehensive literature search was conducted and relevant studies from January 1985 to November 2009 were identified using the electronic database search engines CINAHL, Cochrane Library, EMBASE, MEDLINE, Scopus and Web of Science. Evidence statements, recommendations, practical considerations and research recommendations were developed. Results Fifteen research papers were critically appraised and the evidence formed the basis of these guidelines. Although corticosteroids appear to be more effective, enteral nutrition (elemental or non-elemental) can be offered as an alternative option to induce disease remission. After a course of enteral nutrition, food re-introduction diets may be useful to structure food re-introduction and help maintain disease remission. Dietary fibre is contraindicated in the presence of strictures as a result of the risk of mechanical obstruction. The use of probiotics and prebiotics is not currently supported. Conclusions As an alternative to corticosteroids, evidence supports enteral nutrition to induce disease remission. Food re-introduction diets provide structure to food re-introduction and help maintain disease remission. These guidelines aim to reduce variation in clinical practice. [ABSTRACT FROM AUTHOR]

Using a novel cysteine thiol labeling strategy coupled with mass spectrometric analysis, we identified and quantified the changes in global reversible cysteine oxidation of proteins in the left ventricle of hearts from mice with metabolic syndrome-associated diastolic dysfunction. This phenotype was induced by feeding a high-fat, high-sucrose, type-2 diabetogenic diet to C57BL/6J mice for 8 mo. The extent of reversible thiol oxidation in relationship to the total available (free and reducible) level of each cysteine could be confidently determined for 173 proteins, of which 98 contained cysteines differentially modified ≥1.5-fold by the diet. Our findings suggest that the metabolic syndrome leads to potentially deleterious changes in the oxidative modification of metabolically active proteins. These alterations may adversely regulate energy substrate flux through glycolysis, β-oxidation, citric acid (TCA) cycle, and oxidative phosphorylation (oxphos), thereby contributing to maladaptive tissue remodeling that is associated with, and possibly contributing to, diastolic left ventricular dysfunction. [ABSTRACT FROM AUTHOR]

We have compared fetal heart rate patterns, Apgar scores and umbilical cord gas values following initiation of labour analgesia using either combined spinal-epidural or epidural. One hundred and fifteen healthy women requesting neuraxial analgesia in the first stage of labour were randomly assigned to receive either combined spinal-epidural (n = 62) or epidural analgesia (n = 53). Fetal heart rate traces, recorded for 30 min before and 60 min after neuraxial block, were categorised as normal, suspicious or pathological according to national guidelines. Sixty-one fetal heart rate tracings were analysed in the combined spinal-epidural group and 52 in the epidural group. No significant differences were found in fetal heart rate patterns, Apgar scores or umbilical artery and vein acid-base status between groups. However, in both combined spinal-epidural and epidural groups, there was a significant increase in the incidence of abnormal fetal heart rate patterns following neuraxial analgesia (p < 0.0001); two before compared with eight after analgesia in the combined spinal-epidural group and zero before compared with 11 after in the epidural group. These changes comprised increased decelerations (p = 0.0045) (combined spinal-epidural group nine before and 14 after analgesia, epidural group four before and 16 after), increased late decelerations (p < 0.0001) (combined spinal-epidural group zero before and seven after analgesia, epidural group zero before and eight after), and a reduction in acceleration rate (p = 0.034) (combined spinal-epidural group mean (SD) 12.2 (6.7) h−1 before and 9.9 (6.1) h−1 after analgesia, epidural group 11.0 (7.3) h−1 before and 8.4 (5.9) h−1 after). These fetal heart rate changes did not affect neonatal outcome in this healthy population. [ABSTRACT FROM AUTHOR]

The diets of four common mesopredator fishes were examined in the back-reef habitat of a subtropical fringing reef system during the summer months. Quantitative gut content analyses revealed that crustaceans, represented >60% of ingested prey (% mass) by the latticed sand-perch Parapercis clathrata, brown dottyback Pseudochromis fuscus and half-moon grouper Epinephelus rivulatus. Dietary analyses also provided insights into ontogenetic shifts. Juvenile P. fuscus ingested large numbers of crustaceans (amphipods and isopods); these small prey were rarely found in larger individuals (<1% of ingested mass). Fishes also made an important contribution to the diets of all three species representing 10-30% of ingested mass. Conversely, the sand lizardfish Synodus dermatogenys fed exclusively on fishes including clupeids, gobies and labrids. Differences in the gut contents of the four species recorded were not apparent using stable isotope analysis of muscle tissues. The similarity of δ13C values in muscle tissues suggested that carbon within prey was derived from primary producers, with comparable carbon isotope signatures to corals and macroalgae, whilst similarities in δ15N values indicated that all four species belonged to the same trophic level. Thus, interspecific differences between mesopredator diets were undetectable when using stable isotope analysis which suggests that detailed elucidation of trophic pathways requires gut content analyses. [ABSTRACT FROM AUTHOR]

The family support needs of parents with an intellectual disability ( ID) are relatively unknown. This paper reviewed two types of intervention for parents with ID: those designed to strengthen social relationships and those teaching parenting skills. A literature search was conducted using electronic databases and a limited number of evaluative studies were found. The evidence for interventions aimed at strengthening social relationships was inconclusive; although positive changes were observed, there were limitations in study design which restricted the generalizability of the results. The evidence for parental skills teaching suggested that behavioural based interventions are more effective than less intensive forms such as lesson booklets and the provision of normal services, although these studies also had limitations. There is a need for further large scale controlled studies in this area to provide clearer evidence and to explore additional factors relating to child, parent and family which may impact on outcomes. [ABSTRACT FROM AUTHOR]

Background Eosinophilia is a marker of corticosteroid responsiveness and risk of exacerbation in asthma; although it has been linked to submucosal matrix deposition, its relationship with other features of airway remodelling is less clear. Objective The aim of this study was to investigate the relationship between airway eosinophilia and airway remodelling. Methods Bronchial biopsies from subjects ( n = 20 in each group) with mild steroid-naïve asthma, with either low (0-0.45 mm−2)) or high submucosal eosinophil (23.43-46.28 mm−2) counts and healthy controls were assessed for in vivo epithelial damage (using epidermal growth factor receptor staining), mucin expression, airway smooth muscle ( ASM) hypertrophy and inflammatory cells within ASM. Results The proportion of in vivo damaged epithelium was significantly greater ( P = 0.02) in the high-eosinophil (27.37%) than the low-eosinophil (4.14%) group. Mucin expression and goblet cell numbers were similar in the two eosinophil groups; however, MUC-2 expression was increased ( P = 0.002) in the high-eosinophil group compared with controls. The proportion of submucosa occupied by ASM was higher in both asthma groups ( P = 0.021 and P = 0.046) compared with controls. In the ASM, eosinophil and T-lymphocyte numbers were higher ( P < 0.05) in the high-eosinophil group than both the low-eosinophil group and the controls, whereas the numbers of mast cells were increased in the high-eosinophil group ( P = 0.01) compared with controls. Conclusion Submucosal eosinophilia is a marker (and possibly a cause) of epithelial damage and is related to infiltration of ASM with eosinophils and T lymphocytes, but is unrelated to mucus metaplasia or smooth muscle hypertrophy. [ABSTRACT FROM AUTHOR]

Grapevine leaves infected with powdery mildew are a source of inoculum for fruit infection. Leaves emerging on a single primary shoot of Vitis vinifera cv. Cabernet Sauvignon were exposed to average glasshouse temperatures of 18°C (0·23 leaves emerging/day) or 25°C (0·54 leaves emerging/day). All leaves on 8-10 shoots with approximately 20 leaves each were inoculated with Erysiphe necator conidia to assess disease severity after 14 days in the 25°C glasshouse. Two photosynthetic 'source' leaves per shoot on the remaining 8-10 shoots were treated with 14 CO2 to identify, by autoradiography, the leaf position completing the carbohydrate sink-to-source transition. There was a clear association between the mean modal leaf position for maximum severity of powdery mildew (position 3·7 for 18°C; position 4·4 for 25°C) and the mean position of the leaf completing the sink-to-source transition (position 3·8 for 18°C; position 4·7 for 25°C). The mean modal leaf position for the maximum percentage of conidia germinating to form secondary hyphae was 4·2 for additional plants grown in the 25°C glasshouse. A higher rate of leaf emergence resulted in a greater proportion of diseased leaves per shoot. A Bayesian model, consisting of component models for disease severity and leaf ontogenic resistance, had parameters representing the rate and magnitude of pathogen colonization that differed for shoots developing in different preinoculation environments. The results support the hypothesis that the population of leaves in a vineyard capable of supporting substantial pathogen colonization will vary according to conditions for shoot development. [ABSTRACT FROM AUTHOR]

IL-33, a proposed alarmin, stimulates innate immune cells and Th2 cells to produce IL-13 and is rapidly upregulated upon antigen exposure in murine helminth infection. The human IL-33 response to helminth antigen was analysed in Malians infected with Schistosoma haematobium by disrupting parasite integrity via chemotherapy. Plasma IL-33 was measured pretreatment, and 24 h and 9 weeks post-treatment. At 24 h post-treatment, IL-33 levels were low. Nine week post-treatment IL-33 levels were elevated and were associated with an increase in intracellular IL-13 in eosinophils. Up-regulation of intracellular IL-13 in eosinophils was also associated with eosinophil expression of ST2L, the IL-33 receptor. IL-33 may play an important downstream role in the human response to schistosome adult worm antigen exposure. [ABSTRACT FROM AUTHOR]