Search

Due to the high blood glucose and low oxygen environment in diabetes wounds, complex pathological problems arise, such as failure to upregulate healing factors, biological barriers generation, microvascular lesions, and skin neuropathy. Inspired by nature and traditional Chinese paper‐cutting technology, polypeptide deoxyribonucleic acid (DNA) hydrogel microneedles (MNs) (P‐DNA gel MNs) are prepared. The obtained P‐DNA gel MNs possess optimized structure and large deformability, providing excellent clamping, stability, skin penetration, large deformation, tissue adhesion, and antibacterial properties. By incorporating extracellular vesicles (EVs) extracted under hypoxia (H‐EVs‐hypoxia), P‐DNA gel MNs can regulate tissue microenvironment, scavenge free radicals, and reduce inflammation. Notably, P‐DNA gel MNs integration system activates immune regulation and beneficial pathways, promoting neurogenesis and angiogenesis, reducing pathological pain, and achieving high‐quality healing. Overall, P‐DNA gel MNs system presents a fundamental mechanism for clinical transformation in treating diabetes wounds. [ABSTRACT FROM AUTHOR]

There are issues and challenges in treating deep burns because of the long recovery time, frequent dressing changes, wound infection, and easy to form scar that influence aesthetics. Besides, some specific tissues are not suitable for large dressing coverage (e.g., face, perineum). Therefore, an ideal deep burn dressing should have good adhesion properties to fit the wound effectively, painless and quick replacement, resistant to infection, accelerate wound healing, reduce scarring and facilitate monitoring and diagnosis. Herein, an anti‐dehydration and rapid‐trigger multifunctional hydrogel dressing is prepared by interface reaction. The Pacrylamide‐Formylboronicacid‐Tannic acid (PAFT) hydrogels are prepared by a simple method on anti‐dehydration elastomeric membrane, which is obtained using tannic acid as a dynamic cross‐linking agent with 3‐formylboronic acid and acrylamideunder UV light. The hydrogel exhibits a strong interfacial adhesion (892 J m−2 ± 65 J m−2), which rapidly (2 min) decreases (to 180 J m−2 ± 20 J m−2) when in the presence of glucose solution. The hydrogel has excellent anti‐dehydration and moisturizing properties, and also exhibits superior antibacterial properties, hemostasis, and biocompatibility. This hydrogel is transparent allowing effective observation of wound transformation and therapeutics. Moreover, PAFT hydrogel accelerates the healing of deep burns and reduces scars. [ABSTRACT FROM AUTHOR]

A grade 3 burn is a nonstatic fatal injury, which can lead to complete damage to the skin structure, accompanied by a series of symptoms such as persistent inflammation, pain, pruritus, ulcer, and peripheral neuropathy. Although the primary clinical burn treatment is skin grafting, it cannot comprehensively solve burn symptoms. Here, a multifunctional DNA hydrogel integrated system is conveniently obtained through dynamic cross‐linking of the DNA unit, polyacrylamide, and l‐ascorbate 2‐phosphate (l‐A2P) formation of dense hydrogen bonds. The DNA hydrogel is doped with borneol for pain and itch relief. The obtained DNA hydrogel provides a hotbed similar to the extracellular matrix structure in vivo for the growth and development of stem cells, which can regulate cell proliferation, maintain cell viability, and achieve perfect release in a suitable environment. Additionally, the pharmacological wound dressing shell features excellent mechanical behavior, tissue adhesion, and antibacterial properties. Beyond that, the multifunctional DNA hydrogel integrated system can promote macrophage transformation, angiogenesis, and neurogenesis. Notably, the system activates the phosphatidylinositol 3′‐kinase (PI3K)‐Akt signaling pathway, which helps to promote tissue regeneration. Therefore, the DNA hydrogel integrated system opens the cascade mode of effective integrated treatment of burn wounds, further driving the in‐depth study of clinical transformation mechanisms in the future. [ABSTRACT FROM AUTHOR]

The fundamental physical features such as the mechanical properties and microstructures of the uterus need to be considered when building in vitro culture platforms to mimic the uterus for embryo implantation and further development but have long been neglected. Here, a uterus‐inspired niche (UN) constructed by grafting collagen gels onto polydimethylsiloxane based on a systematic investigation of a series of parameters (varying concentrations and thicknesses of collagen gel) is established to intrinsically specify and simulate the mechanics and microstructures of the mouse uterus. This brand‐new and unique system is robust in supporting embryo invasion, as evidenced by the special interaction between the embryos and the UN system and successfully promoting E3.5 embryo development into the early organogenesis stage. This platform serves as a powerful tool for developmental biology and tissue engineering. [ABSTRACT FROM AUTHOR]

Currently, diabetic infectious wound treatments remain a significant challenge for regenerative medicine due to the unicity of clinical dressings, which lack systemic multifunctional wound dressings with high absorbability, customizable shape, rapid self‐healing, guiding tissue regeneration, and restoring physiological functions. Here, a multifunctional DNA hydrogel is conveniently obtained through grafting DNA units and polyethyleneimine dynamic cross‐linking and doped heating function black phosphorus quantum dots. The obtained DNA hydrogel features excellent exudate absorption performance, adjustable heating ability, mechanical behavior, self‐healing ability, writability, tissue adhesion, and antibacterial properties. The incorporation of procyanidin B2 (OPC B2) endows the DNA hydrogels with renowned scavenging free radicals and antioxidant properties. Furthermore, the DNA hydrogel dressing can promote the transformation of macrophages from pro‐inflammatory M1 into repairing M2 phenotype, keeping the wound in a stable remodeled state. Astonishingly, the DNA hydrogel dressing can activate neurons to transform into a repair state, accelerating skin nerve regeneration and angiogenesis. Beyond that, it can recruit myeloid cells to activate the adaptive immune response, enhancing the ability of DNA hydrogel dressing to promote tissue regeneration, thereby promoting hair follicle and hair regeneration. Therefore, this advanced collaborative strategy provides an effective method for cascade management of clinical guided tissue regeneration. [ABSTRACT FROM AUTHOR]

The telencephalon is also known as the cerebrum, and it consists of the largest part of the brain. It makes up about 85% of the total weight of the brain. Neuroglobin (Ngb) is a protein found in neurons of both the peripheral and central nervous system that appears to convey some resilience to hypoxia, while the hypoxia‐inducible factor (Hif‐1α) is a dimeric protein complex that plays an integral role in the body's response to low oxygen concentrations, or hypoxia. The study examines the expression of Ngb and Hif‐1α in the telencephalon of adult yak in the telencephalon. The immunohistochemistry (IHC), quantitative real‐time PCR and Western blot (WB) were employed to investigate Ngb and Hif‐1α expression in the telencephalon. Ngb and Hif‐1α are significantly expressed in all tissues of the telencephalon except the hypothalamus. The cerebellar cortex, hippocampus, amygdala, cerebellum and corpus callosum recorded the highest expression but not significant. The overall expression revealed that Ngb expression was higher as compared to Hif‐1α. The IHC results also showed that the expression of Ngb and Hif‐1α were higher in the cerebellar cortex, hippocampus, amygdala, cerebellum and corpus callosum as compared to other regions. The results suggested that Ngb and Hif‐1α expression influence the adaptive mechanism of yak to the high altitude environment. Both Ngb and Hif‐1α participate in oxygen transports throughout the telencephalon and have functions in neuroprotection. Further studies are needed to confirm the mechanism of adaptation. [ABSTRACT FROM AUTHOR]

We report core@satellite Janus mesoporous silica‐Pt@Au (JMPA) nanomotors with pH‐responsive multi‐phoretic propulsion. The JMPA nanomotors first undergo self‐diffusiophoretic propulsion in 3.0 % H2O2 due to the isolation of the Au nanoparticles (AuNPs) from the PtNPs layer. Then the weak acidity of H2O2 can trigger the disassembly and reassembly of the AuNPs, resulting in the Janus distribution of large AuNPs aggregates. Such reconstruction of JMPA leads to the contact between PtNPs and AuNPs aggregates, thus changing the propulsion mechanism to self‐electrophoresis. The asymmetric and aggregated AuNPs also enable the generation of a thermal gradient under laser irradiation, which propels the JMPA nanomotors by self‐thermophoresis. Such multi‐phoretic propulsion offers considerable promise for developing advanced nanomachines with a stimuli‐responsive switch of propulsion modes in biomedical applications. [ABSTRACT FROM AUTHOR]

Background: The Saiga antelope (Saiga tatarica) is native to Eurasia and is a member of the family Bovidae. Prior to 1920, the antelope had been extensively hunted for its horns, which were used in traditional Chinese medicine. Since 1920, the Saiga antelope has been protected because of this extensive hunting, which nearly led to its extinction. Objective: The study evaluated haematological and biochemical parameters to provide references for the Calf Saiga antelope (S. tatarica). The study also sought to explore the mechanisms affecting these parameters in both genders of the Calf Saiga antelope. Methods: Haematological and biochemical parameters were collected from the Calf Saiga antelope. Haematological and biochemical parameters were analysed by the Coulter counter and Automatic analyser, respectively. Results: The average concentrations of female triglyceride levels showed significantly higher values than the significant concentrations of male. Female red blood cells and platelets concentrations were statistically significant than the significant concentrations of males. Magnesium female concentrations were also significantly higher than male values. Other parameters showed differences between males and females. Conclusion: The reported results show that haematological and biochemical characteristics varied among Calf Saiga antelope and other animals. The study results suggest that regardless of the factors, breed, the breeding environment, and climatic variables, haematological and biochemical variations can be triggered that can result in a reduction in the heat production needed for maintenance of homeothermy. [ABSTRACT FROM AUTHOR]

The abnormalities of early post‐implantation embryos can lead to early pregnancy loss and many other syndromes. However, it is hard to study embryos after implantation due to the limited accessibility. The success of embryo culture in vitro can avoid the challenges of embryonic development in vivo and provide a powerful research platform for research in developmental biology. The biophysical and chemical cues of the microenvironments impart significant spatiotemporal effects on embryonic development. Here, we summarize the main strategies which enable researchers to grow embryos outside of the body while overcoming the implantation barrier, highlight the roles of engineered microenvironments in regulating early embryonic development, and finally discuss the future challenges and new insights of early embryo culture. [ABSTRACT FROM AUTHOR]

Although considerable progress has been made with carbon-based heterogeneous nanocatalysts, great effort is still needed to regulate their structural parameters to further improve their catalytic activities and stabilities. Herein, we have successfully designed and synthesized new composite nanocatalysts composed of porous hollow carbon nanospheres (PHCNs) containing many small Pd nanoparticles (NPs) on their inner surfaces (PHCNs@Pd NPs). Notably, the PHCNs have disordered micropores and large center-radial mesopores in the shell and craterlike inner surface, and a high density of small Pd NPs are partially confined on the inner surfaces of the PHCNs. Dendrimer-like mesoporous silica nanospheres (DMSNs) as hard templates play a critical role in the formation of these unique structures. The resulting PHCNs@Pd NP catalyst exhibits high catalytic activity and selectivity as well as good recyclability in the catalytic oxidation of benzyl alcohol to benzaldehyde, and this catalytic performance is attributed to the unique structure of the support coupled with ultradispersed Pd NPs. Moreover, this synthesis strategy may provide some inspiration for the synthesis of advanced composites with complicated nanostructures for various applications. [ABSTRACT FROM AUTHOR]

A pH-responsive free-blockage release system was achieved through controlling the hydrophobic/hydrophilic conversion of mesoporous silica nanopores. This system further presented pulsatile release with changing pH values between 4.0 and 7.0 for several cycles. This free-blockage release system could also release antitumor agents to induce cell death after infecting tumor cells and could have the ability of continuous infection to tumor cells with high drug-delivery efficiency and few side effects. [ABSTRACT FROM AUTHOR]

A selective release system was demonstrated with a dual-cargo loaded MSNs. When stimulated by different signals (UV or H+), this system could selectively release different kinds of cargoes individually. Furthermore, this system has been used to provide a combination of chemotherapy and biotherapy for cancer treatment. This controlled release system could be an important step in the development of more effective and sophisticated nanomedicine and nanodevices, due to the possibility of selective release of a complex multi-drug. [ABSTRACT FROM AUTHOR]

With an optimized photonic crystal, the chemiluminescence intensity is enhanced by 44.9 times and the chemiluminescence emission light intensity decay rate is accelerated 3.2 times. The interaction between the chemiluminescence system and the photonic crystal is discussed. The results will exploit a new platform for the photonic crystal based chemiluminescence assay. [ABSTRACT FROM AUTHOR]