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BACKGROUND: The use of synbiotics is emerging as a promising intervention strategy for regulating the gut microbiota and for preventing or reducing obesity, in comparison with the use of probiotics or prebiotics alone. A previous in vivo study revealed that Lacticaseibacillus paracasei K56 (L. paracasei K56) could alleviate obesity induced in high‐fat‐diet mice; however, the effect of the synbiotic combination of L. paracasei K56 and prebiotics in obese individuals has not been explored fully. RESULTS: The effect of prebiotics on the proliferation of L. paracasei K56 was determined by spectrophotometry. The results showed that polydextrose (PG), xylooligosaccharide (XOS), and galactooligosaccharide (GOS) had a greater potential to be used as substrates for L. paracasei K56 than three other prebiotics (melitose, stachyose, and mannan‐oligosaccharide). An in vitro fermentation model based on the feces of ten obese female volunteers was then established. The results revealed that K56_GOS showed a significant increase in GOS degradation rate and short‐chain fatty acid (SCFA) content, and a decrease in gas levels, compared with PG, XOS, GOS, K56_PG, and K56_XOS. Changes in these microbial biomarkers, including a significant increase in Bacteroidota, Bifidobacterium, Lactobacillus, Faecalibacterium, and Blautia and a decrease in the Firmicutes/Bacteroidota ratio and Escherichia‐Shigella in the K56_GOS group, were associated with increased SCFA content and decreased gas levels. CONCLUSION: This study demonstrates the effect of the synbiotic combination of L. paracasei K56 and GOS on obese individuals and indicates its potential therapeutic role in obesity treatment. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Genome‐scale metabolic models and flux balance analysis (FBA) have been extensively used for modeling and designing bacterial fermentation. However, FBA‐based metabolic models that accurately simulate the dynamics of coculture are still rare, especially for lactic acid bacteria used in yogurt fermentation. To investigate metabolic interactions in yogurt starter culture of Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus, this study built a dynamic metagenome‐scale metabolic model which integrated constrained proteome allocation. The accuracy of the model was evaluated by comparing predicted bacterial growth, consumption of lactose and production of lactic acid with reference experimental data. The model was then used to predict the impact of different initial bacterial inoculation ratios on acidification. The dynamic simulation demonstrated the mutual dependence of S. thermophilus and L. d. bulgaricus during the yogurt fermentation process. As the first dynamic metabolic model of the yogurt bacterial community, it provided a foundation for the computer‐aided process design and control of the production of fermented dairy products. [ABSTRACT FROM AUTHOR]

Atopic dermatitis (AD) is a recurring allergic skin disease that has a high incidence. Orally applied Bifidobacteria ameliorate signs of irritated skin and enhance the skin barrier. The present study investigated the safety and efficacy of a topically used cell‐free culture supernatant (CFS) from a Bifidobacterium infantis strain using in vitro evaluation methods. The results showed that CFS had strong free radical scavenging activity on DPPH, ABTS, ·OH and O2‐radicals. CFS treatment fundamentally reduced the intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) contents and improved the activities of antioxidant enzymes (CAT, SOD and GSH‐Px) in H2O2‐treated HaCaT cells. Notably, the upregulation of skin physical barrier gene (FLG, LOR, IVL, AQP3 and TGM1) expression and skin antimicrobial peptide gene (CAMP, hBD‐2 and hBD‐3) expression by CFS might contribute to skin barrier resistance. CFS was non‐irritating to the skin and eyes. CFS from the Bifidobacterium infantis strain had strong antioxidant properties on the skin and strengthened skin barrier function, and it was safe for topical use. [ABSTRACT FROM AUTHOR]

Foodborne protein hydrolysates exhibit biological activity that may be therapeutic in a number of human disease settings. Hemp peptides (HP) generated by controlled hydrolysis of hemp proteins have a number of health benefits and are of pharmaceutical value. In the present study, we produce small molecular weight HP from hemp seed and investigate its anticancer properties in Hep3B human liver cancer cells. We demonstrate that HP treatment increased apoptosis, reduced cell viability, and reduced cell migration in Hep3B human liver cancer cells without affecting the normal liver cell line L02. We correlate these phenotypes with increased cellular ROS levels, upregulation of cleaved caspase 3 and Bad, and downregulation of antiapoptotic Bcl‐2. HP treatment led to increased Akt and GSK‐3β phosphorylation, with subsequent downregulation of β‐catenin, suggesting β‐catenin signaling modulation as a critical mechanism by which HP exhibits anticancer properties. Our findings suggest HP are of potential therapeutic interest for liver cancer treatment. [ABSTRACT FROM AUTHOR]

Skeletal muscle atrophy is a common and serious complication of chronic kidney disease (CKD). Oxidative stress and autophagy are the primary molecular mechanisms involved in muscle atrophy. Calycosin, a major component of Radix astragali, exerts anti‐inflammatory, anti‐oxidative stress and anti‐autophagy effects. We investigated the effects and mechanisms of calycosin on skeletal muscle atrophy in vivo and in vitro. 5/6 nephrectomy (5/6 Nx) rats were used as a model of CKD. We evaluated bodyweight and levels of serum creatinine (SCr), blood urea nitrogen (BUN) and serum albumin (Alb). H&E staining, cell apoptosis, oxidative stress biomarkers, autophagosome and LC3A/B levels were performed and evaluated in skeletal muscle of CKD rat. Calycosin treatment improved bodyweight and renal function, alleviated muscle atrophy (decreased the levels of MuRF1 and MAFbx), increased superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH‐Px) activity and reduced malondialdehyde (MDA) levels in skeletal muscle of CKD rats. Importantly, calycosin reduced autophagosome formation, down‐regulated the expression of LC3A/B and ATG7 through inhibition of AMPK and FOXO3a, and increased SKP2, which resulted in decreased expression of CARM1, H3R17me2a. Similar results were observed in C2C12 cells treated with TNF‐α and calycosin. Our findings showed that calycosin inhibited oxidative stress and autophagy in CKD induced skeletal muscle atrophy and in TNF‐α‐induced C2C12 myotube atrophy, partially by regulating the AMPK/SKP2/CARM1 signalling pathway. [ABSTRACT FROM AUTHOR]

Oral mesenchymal stem/progenitor cells (MSCs) are renowned in the field of tissue engineering/regeneration for their multilineage differentiation potential and easy acquisition. These cells encompass the periodontal ligament stem/progenitor cells (PDLSCs), the dental pulp stem/progenitor cells (DPSCs), the stem/progenitor cells from human exfoliated deciduous teeth (SHED), the gingival mesenchymal stem/progenitor cells (GMSCs), the stem/progenitor cells from the apical papilla (SCAP), the dental follicle stem/progenitor cells (DFSCs), the bone marrow mesenchymal stem/progenitor cells (BM-MSCs) from the alveolar bone proper, and the human periapical cyst-mesenchymal stem cells (hPCy-MSCs). Apart from their remarkable regenerative potential, oral MSCs possess the capacity to interact with an inflammatory microenvironment. Although inflammation might affect the properties of oral MSCs, they could inversely exert a multitude of immunological actions to the local inflammatory microenvironment. The present review discusses the current understanding about the immunomodulatory role of oral MSCs both in periodontitis and systemic diseases, their "double-edged sword" uniqueness in inflammatory regulation, their affection of the immune system, and the underlying mechanisms, involving oral MSC-derived extracellular vesicles. [ABSTRACT FROM AUTHOR]

Long noncoding RNA Breast Cancer Antiestrogen Resistance 4 (BCAR4) has been identified to be oncogenic in several cancers. In our study, we demonstrated that BCAR4 expression was significantly upregulated in glioma tissues compared with paired nontumor tissues. In addition, higher BCAR4 level was associated with poor overall survival in patients with glioma. Besides, we also discovered that knockdown of BCAR4 inhibited cell proliferation, whereas BCAR4 overexpression promoted this process. Intriguingly, we proved a cellular transformation of normal human astrocyte cells (NHAs) in response to enforced expression of BCAR4. In addition, we revealed that BCAR4 affected cell proliferation in glioma cells by promoting cell cycle progression and inhibiting cell apoptosis. Mechanistically, we uncovered that BCAR4 activated PI3K/AKT signaling pathway in glioma through upregulating EGFR and interacting with it. Moreover, activating PI3K/AKT pathway could reverse the repressive effects caused by BCAR4 silence on the biological behaviors of glioma cells, whereas inhibition of this pathway rescued the impact of BACR4 upregulation in NHAs. These findings disclosed that BCAR4 contributes to glioma progression by enhancing cell growth via activating EGFR/PI3K/AKT pathway, providing potent evidence that BCAR4 could be an effective new target for treatment and prognosis of glioma patients. [ABSTRACT FROM AUTHOR]

Herein, we report the supramolecular self‐assembly of fully substituted cyclopentano cucurbit[5]uril (CyP5Q[5]) and cucurbit[6]uril (CyP6Q[6]) with Cu(ClO4)2 and Zn(ClO4)2 in a solution of formic acid. Single crystal X‐ray diffraction analysis showed that CyP5Q[5]‐Cu(ClO4)2 forms a W‐type molecular capsule complex and CyP5Q[5]‐Zn(ClO4)2 forms alternate Z‐type and V‐type molecular capsule complexes. Both CyP6Q[6]‐Cu(ClO4)2 and CyP6Q[6]‐Zn(ClO4)2 form Z‐type molecular capsule complexes. These two coordination systems have the same unit cell parameters and are isomorphic complexes. In these four systems the ClO4– ions are coordinated around the cucurbituril via ion‐dipole interactions and induce the self‐assembly of the cucurbituril and metal ions. [ABSTRACT FROM AUTHOR]

To address the 3D point matching problem where the pose difference between two point sets is unknown, the authors propose a path following (PF)-based algorithm. This method works by reducing the objective function of robust point matching (RPM) algorithm to a function of point correspondence variable and then using PF for optimisation. By using the 3D similarity transformation which has few parameters, authors' method needs no regularisation on transformation and, therefore, can handle the case when the pose difference between two point sets is unknown. The authors also propose a novel convex term for use in the PF algorithm which is based on the low-rank nature of authors' objective function and leads to a PF algorithm which converges quickly. Experimental results demonstrated better robustness of the proposed method over state-of-the-art methods and authors' method is also efficient. [ABSTRACT FROM AUTHOR]

The mine ventilation system is most important and technical measure for ensuring safety production in mines. The structural complexity of a mine ventilation network can directly affect the safety and reliability of the underground mining system. Quantitatively justifying the degree of complexity can contribute to providing a deeper understanding of the essential characteristics of a network. However, so far, there is no such a model which is able to simply, practically, reasonably, and quantitatively determine or compare the structural complexity of different ventilation networks. In this article, by analyzing some typical parameters of a mine ventilation network, we conclude that there is a linear functional relationship among five key parameters (number of ventilation network branches, number of nodes, number of independent circuits, number of independent paths, and number of diagonal branches). Correlation analyses for the main parameters of ventilation networks are conducted based on SPSS. Based on these findings, a new evaluation model for the structural complexity of ventilation network (which is represented by C) has been proposed. By combining SPSS classification analyses results with the characteristics of mine ventilation networks, standards for the complexity classification of mine ventilation systems are put forward. Using the developed model, we carried out analyses and comparisons for the structural complexity of ventilation networks for typical mines. Case demonstrations show that the classification results correspond to the actual situations. © 2014 Wiley Periodicals, Inc. Complexity 21: 21-34, 2015 [ABSTRACT FROM AUTHOR]

The present results demonstrated that high glucose (G), salt (S), and cholesterol C (either alone or in combination), as mimicking extracellular changes in metabolic syndrome, damage cardiomyocyte-like H9c2 cells and reduce their viability in a time-dependent manner. However, the effects were greatest when cells were exposed to all three agents (GSC). The mRNA of glycoprotein (gp) 130 and WSX-1, both components of the interleukin (IL)-27 receptor, were present in H9c2 cells. Although mRNA expression was not affected by exogenous treatment with IL-27, the expression of gp130 mRNA (but not that of WSX-1 mRNA) was attenuated by GSC. Treatment of IL-27 to H9c2 cells increased activation of signal transducer and activator of transcription 3 (STAT3) and protected cells from GSC-induced cytochrome c release and cell damage. The protective effects of IL-27 were abrogated by the STAT3 inhibitor, stattic. The results of the present study clearly demonstrate that the STAT3 pathway triggered by anti-inflammatory IL-27 plays a role in protecting cardiomyocytes against GSC-mediated damage. [ABSTRACT FROM AUTHOR]

A facile solid-phase extraction (SPE) method based on bare Fe3O4 magnetic nanoparticles (Fe3O4 NPs) was developed to capture cinnamic acid (CA) from a well-known traditional Chinese medicine, Ramulus cinnamomi, for high performance liquid chromatography (HPLC) analysis. The as-prepared Fe3O4 NPs were characterized via Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM) and vibrating sample magnetometer (VSM). Several parameters that influence CA extraction rate, such as the amount of Fe3O4 NPs, shaking time, methanol content, eluent pH, and elution time, were investigated. The experimental results show that the CA extraction was 86.9 % at optimal condition, and the mass yield of CA extraction was found to be 0.00021 %. This study demonstrates that the developed CA extraction method is simple, cheap and environmentally friendly. Therefore, the proposed method has a potential for CA separation and purification from complex biological samples. [ABSTRACT FROM AUTHOR]

Length-weight relationships ( LWRs) of five fish species commonly associated with Antarctic krill fishery, were determined. Samples were collected with Chinese krill trawler (codend mesh size 2 cm) in the Atlantic sector of the Southern Ocean from January to August 2016. Data on LWRs and the relationships between weight and standard length as well as weight and total length of those species were updated for the database of FishBase. Those data on fish species derived from Antarctic krill fishery will be very helpful in understanding the interaction between krill fishery and the associated fish community. [ABSTRACT FROM AUTHOR]

Abstract: LDH-C4 is a lactate dehydrogenase that catalyzes the interconversion of pyruvate with lactate. In mammals the, Ldh-c gene was originally thought to be expressed only in testis and spermatozoa. Plateau pika (Ochotona curzoniae), belonging to the genus Ochotona of the Ochotonidea family, is a hypoxia tolerant mammal living at 3000–5000 m above sea levelon the Qinghai-Tibet Plateau. We found that the expression pattern of six LDH isoenzymes in the somatic tissues of female and male plateau pikas to be the same as those in testis and sperm, suggesting that LDH-C4 was expressed in somatic tissues of plateau pika. Here we report the detection of LDHC in the somatic tissues of plateau pika using RT-PCR, Western blotting and immunohistochemistry. Our results indicate that Ldh-c mRNA is transcribed in the heart, liver, lung, kidney, brain, skeletal muscle and testis. In somatic tissues LDHC was translated in the cytoplasm, while in testis it was expressed in both cytoplasm and mitochondria. The third band from cathode to anode in LDH isoenzymes was identified as LDH-C4. The finding that Ldh-c is expressed in both somatic tissues and testis of plateau pika provides important implications for more in-depth research into the Ldh-c function in mammals. [Copyright &y& Elsevier]

The surface of Trypanosoma brucei is dominated by glycosyl-phosphatidylinositol (GPI)-anchored proteins, and endocytosis is clathrin dependent. The vast majority of internalized GPI-anchored protein is efficiently recycled, while the processes by which transmembrane domain (TMD) proteins are internalized and sorted are unknown. We demonstrate that internalization of invariant surface glycoprotein (ISG)65, a trypanosome TMD protein, involves ubiquitylation and also requires clathrin. We find a hierarchical requirement for cytoplasmic lysine residues in internalization and turnover, and a single position-specific lysine is sufficient for degradation, surface removal and attachment of oligoubiquitin chains. Ubiquitylation is context dependent as provision of additional lysine residues by C-terminal fusion of neuronal precursor cell-expressed developmentally downregulated protein (NEDD)8 fails to support ubiquitylation. Attachment of NEDD8 leads to degradation by a second ubiquitin-independent pathway. Moreover, degradation of ubiquitylated or NEDDylated substrate takes place in an acidic compartment and is proteosome independent. Significantly, in non-opisthokont lineages, Rsp5p or c-Cbl, the E3 ubiquitin ligases acting on endocytic cargo, are absent but Uba1 class genes are present and are required for cell viability and ISG65 ubiquitylation. Hence, ubiquitylation is an evolutionarily conserved mechanism for internalization of surface proteins, but aspects of the machinery differ substantially between the major eukaryotic lineages. [ABSTRACT FROM AUTHOR]

A letter to the editor is presented that comments on the treatment of methimazole-induced agranulocytosis with low-dose prednisone and ciclosporin.

To address feature matching problem where there is only partial overlap between the two feature sets, minimisation of an energy function consisting of feature matching costs and a concave regularisation term capable of handling partial overlaps is proposed. To improve robustness to disturbances, a path following strategy for minimisation which dynamically combines weights of the two terms is adopted. Experimental results verify better performance of the method over several state-of-the-art methods. [ABSTRACT FROM AUTHOR]

A new non-rigid feature point matching algorithm is proposed, where the non-rigid global deformation is preserved by dividing the object into several overlapping local regions and only regularising the deformations of the local regions. A key property of the proposed algorithm is that the objective function is concave. By combining with the total unimodularity of the constraints, the proposed algorithms can be proved to directly yield integer valued solutions. Experimental results demonstrate the better robustness of the method over several state-of-the-art methods. [ABSTRACT FROM AUTHOR]

Analyzes the crystallization and preliminary X-ray crystallographic studies on recombinant rat choline acetyltransferase. Unit-cell parameters of the crystals which were shown to belong to an orthorhombic space group; Packing considerations indicating there to be one molecule per asymmetric unit.

The dinuclear title compound, [Pb(2)(C(6)H(8)O(4))(NO(3))(2)(C(19)H(13)N(5))(2)], lies with the mid-point of the butyl chain of the bridging adipate unit on a center of inversion. The Pb(II) ion is covalently bonded to the nitrate anion and is bonded to a carboxyl-ate group of the adipate unit by another covalent bond. The N-heterocycle functions in a chelating tridentate mode. The metal atom exists in a Ψ-octa-hedral coordination environment. When weaker Pb⋯O inter-actions are also considered, the geometry is a Ψ-tricapped trigonal prism in which the lone-pair electrons occupy one face of the trigonal prism. Adjacent mol-ecules are linked into a layer structure by N-H⋯O hydrogen bonds.