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Variations in surface air temperature on the eastern Tibetan Plateau (TP‐SAT) during boreal summer, related to Barents‐Kara spring sea ice and summer Arctic Oscillation (AO), are investigated using observations and ERA‐5 reanalysis data during 1979–2018. Results show that the primary mode of summer eastern TP‐SAT (SAT_EOF1) is characterized by the consistent warming pattern with larger values over the northeastern TP, which is linked with the anticyclonic circulation located in the northeastern flank of the TP. This anticyclone enhances westward water vapor transport, warm temperature advection, and causes descending over the eastern TP, Descending further results in deceased middle level cloud cover and increased solar radiation, accordingly, favoring the eastern TP warming. Further statistical analysis reveals a robust connection between Barents‐Kara spring sea ice, summer AO and SAT_EOF1. Moreover, anticyclonic anomaly located in the northeastern region of the TP acts as an important driver of this consistent warming pattern over the eastern TP, which is modulated by two individual Rossby wave trains from the northwestern Russia and northwestern Atlantic. The spring sea ice in Barents‐Kara could cause decreased precipitation and reduced soil moisture in northwestern Russia during following summer, which further induces the Eurasian‐like teleconnection and affects the anticyclonic anomaly situated in the northeastern flank of the TP, thereby favoring eastern TP warming. In addition, from northwestern Atlantic to northeastern region of the TP, the Rossby wave train is mainly affected by summer AO, with positive AO causing anticyclonic anomaly over northeast of the TP, thus contributing to the TP warming. Plain Language Summary: Tibetan Plateau (TP) is a region that is easily affected by the global warming. Numerous studies have extensively documented a remarkable warming trend in the TP during boreal summer, particularly noticeable since the late 1990s. Despite these valuable observations, its mechanisms are not yet clear. In this study, we found that the anticyclonic anomaly over the northeast of TP is a crucial system affecting eastern TP warming in boreal summer. This anticyclonic anomaly leads to the strengthened westward water vapor transport and causes descending over the eastern TP. Descending, in turn, results in the reduction of middle level cloud cover and increased solar radiation. Further analysis indicated that this anomalous anticyclone is mainly modulated by two individual wave trains from the northwestern Russia and northwestern Atlantic, respectively. From northwestern Atlantic to northeastern flank of the TP, the wave train is mainly affected by the summer AO, and another is mainly driven by the spring Barents‐Kara sea ice. Those two factors account for about 36% of the summer eastern TP warming. Key Points: Anticyclonic anomaly located in the northeastern flank of the TP favors the eastern TP warmingSpring Barents‐Kara sea ice could affect this anticyclone through its influences on summer soil moisture over northwestern RussiaThis anticyclone is also modulated by the wave train from northwestern Atlantic, which is mainly affected by Arctic Oscillation [ABSTRACT FROM AUTHOR]

In this paper, a honeycomb Si3N4 ceramic was fabricated by 3D printing with a well‐preserved structure. The effects of Si3N4 content on the rheological properties of Si3N4/sol–silica ink and the printing resolution of products were investigated. The microstructure, phase composition, liner shrinkage rate, and fracture behavior of printed samples before and after sintering were systematically characterized in detail. The results showed that the modified inks had the optimized rheological properties, and the stress–shear rate curves corresponding to each slurry could be well described by Bingham and Herschel–Bulkley fluid models. The corresponding slump rates of the printed samples with different Si3N4 to sol–silica mass ratios were all lower than 4%, and the linear shrinkage rate of all of the samples after sintering was below 20%. The fracture behavior under compressive loading of the honeycomb Si3N4 ceramics tended to be non‐catastrophic fractures both before and after sintering. The compressive strengths of all of the printed samples decreased with the increase of the Si3N4 content, and the highest compressive strength of the honeycomb ceramics could reach 131.2 MPa after sintering at 1600°C, which was about 366.9% higher than that of the samples in green state prior to the sintering. [ABSTRACT FROM AUTHOR]

The magnitude and spatial pattern of anomalous net biome exchange (NBE) induced by the 2015/16 El Niño over Amazonian rainforests remain uncertain. We here investigated them using multi‐model posterior NBE products in the Orbiting Carbon Observatory‐2 (OCO‐2) version 10 modeling intercomparison project. Results suggest that relative to the annual NBE average in 2017/18, larger anomalous carbon release occurred over the eastern and northern Amazonian rainforests in 2015/16, with a total flux of approximately 0.4 PgC yr−1 after assimilating satellite‐observed column CO2 concentrations (XCO2) over land. We further find that this anomalous spatial pattern was predominantly determined by soil dryness, while the total positive NBE anomaly was dominated by higher temperature with its contribution of approximately 68～70%. We believe that atmospheric inversions assimilating more satellite‐observed XCO2 in future can provide us more comprehensive understanding how Amazonian rainforests cope with the abiotic stresses induced by El Niño events. Plain Language Summary: Interannual variability of carbon flux associated with its drivers over Amazonian rainforests are not fully understood. We here used several groups' newly available posterior CO2 flux estimates to comprehensively investigate the net carbon flux anomaly induced by the 2015/16 extreme El Niño. A total net carbon flux anomaly of approximately 0.4 PgC yr−1 was estimated, which showed larger carbon release over the eastern and northern Amazonian rainforests. We further suggest that although dry conditions greatly shaped the spatial pattern of the anomalous carbon flux, the total carbon flux anomaly was controlled by the higher temperature, with its contribution of approximately 68∼70%. Key Points: Net biome exchange (NBE) anomalies over Amazonian rainforests induced by the 2015/16 El Niño were investigated based on multiple atmospheric inversionsThe spatial pattern of NBE anomaly was regulated by soil water with larger anomalies over the eastern and northern Amazonian rainforestsThe total NBE anomaly was estimated at about 0.4 PgC yr−1 in 2015/16 relative to the average in 2017/18, dominated by higher temperature [ABSTRACT FROM AUTHOR]

The 2019 extreme positive Indian Ocean dipole drove climate extremes over Indian Ocean rim countries with unclear carbon‐cycle responses. We investigated its impact on net biome productivity (NBP) and its constituent fluxes, using the Global Carbon Assimilation System (GCASv2) product, process‐based model simulations from TRENDYv9, and satellite‐based gross primary productivity (GPP). By distinguishing two separate regions, the India‐Africa and Asia‐Pacific, GCASv2 indicated enhanced terrestrial carbon uptake of 0.23 ± 0.20 PgC and release of 0.38 ± 0.15 PgC, respectively, during September–December (SOND) 2019. These NBP anomalies had comparable magnitudes to those following the 2015 extreme El Niño which, however, caused the consistent carbon release in both regions. The TRENDYv9 model ensemble confirmed these NBP responses, albeit with smaller magnitudes. These regional NBP anomalies were related to soil moisture variations with a dominant role of GPP. Understanding the impact of IOD provides new insights into mechanisms driving interannual variations in regional carbon cycling. Plain Language Summary: The extreme Indian Ocean Dipole (IOD) can drive climate extremes, such as floods, heatwaves, droughts, and wildfires, over the Indian Ocean rim countries. However, responses of regional terrestrial carbon cycling to IOD remained unclear. We used the net biome productivity (NBP) from an atmospheric inversion and multiple terrestrial biosphere models to demonstrate an enhanced terrestrial carbon uptake and release over the India‐Africa and Asia‐Pacific regions, respectively, during the extreme positive IOD (September–December) in 2019. These IOD‐induced regional NBP anomalies showed comparable magnitudes but different patterns to those following the 2015 extreme El Niño. Along with the more frequent extreme IOD under future greenhouse warming, IOD will be an important mechanism driving interannual variations in regional carbon cycling. Key Points: The 2019 extreme positive Indian Ocean Dipole caused the enhanced land carbon uptake over India‐Africa and release over Asia‐Pacific during September–DecemberThese regional net biome productivity (NBP) anomalies were closely related to soil moisture variations with a dominant role of gross primary productivityThese Indian Ocean Dipole‐induced regional NBP anomalies showed comparable magnitudes but different patterns to those following the 2015 extreme El Niño [ABSTRACT FROM AUTHOR]

Precipitation, a key determinant of soil moisture variations, plays an important role in regulating terrestrial carbon fluxes on multiple time scales. It is a critical meteorological forcing to drive terrestrial biosphere model (TBM), however, with a large uncertainty itself. We here investigated to what extent precipitation alone can cause uncertainties of model‐simulated carbon flux from terrestrial ecosystems to atmosphere (FTA), based on eight precipitation products and a TBM, VEGAS. We find that the pattern of uncertainties in simulated FTA obviously differs from the pattern of discrepancies in precipitation, owing to divergent water sensitivities of vegetation over different regions. Globally, the uncertainty in FTA can be up to approximately 40.73% of the uncertainty in TRENDYv6 multi‐model simulated FTA which is caused by model structural and parameter uncertainty. A good linear relationship emerges between global area‐averaged land climatological annual precipitation and simulated total FTA with the slope of −0.0040 PgC yr−1 per mm yr−1 (p = 0.03; negative for carbon sink), where 70% is explained by the sensitivity over extra‐tropical Northern Hemisphere (NH). For seasonal cycle, compared to nearly constant inter‐precipitation spreads over tropics plus extra‐tropical southern hemisphere (Trop + SH), uncertainties in corresponding simulated FTA show obvious seasonal differences with the relatively larger uncertainties in March‐April‐May (MAM) and August‐September‐October (ASO). For interannual variability, uncertainties in simulated total FTA are, albeit smaller, nonnegligible, which are 40.61% (global), 38.17% (Trop + SH), and 29.63% (NH) of the TRENDYv6 inter‐model uncertainty, respectively. Therefore, generating better global precipitation product is important for reducing the uncertainty in simulating terrestrial carbon sinks. Plain Language Summary: Precipitation, a key forcing in terrestrial biosphere model (TBM), has a large uncertainty itself. We here used eight land precipitation products to run TBM VEGAS and explored the resulting uncertainties in simulating carbon flux from terrestrial ecosystems to atmosphere (FTA) on climatology, seasonal cycle, and interannual variability. In climatology, the uncertainty in global FTA induced by different precipitation can be approximately 40.73% of the uncertainty in TRENDYv6 multi‐model simulations induced by model structural and parameter uncertainty. Furthermore, we find a good linear relationship between global area‐averaged land climatological annual precipitation and simulated total FTA, implying more precipitation over land with stronger carbon sinks, especially over extra‐tropical Northern Hemisphere (NH). For seasonal cycle, uncertainties in simulated FTA over tropics plus extra‐tropical southern hemisphere (Trop + SH) show relatively larger values in March‐April‐May and August‐September‐October. For interannual variability, uncertainties in simulated total FTA are up to 40.61% (global), 38.17% (Trop + SH), and 29.63% (NH) of the TRENDYv6 inter‐model uncertainty, respectively. In general, uncertainty in land precipitation data sets can cause considerable uncertainties in simulating FTA, suggesting the importance of generating the better precipitation product for better simulating the terrestrial carbon sinks. Key Points: Uncertainties in simulating land‐atmosphere carbon fluxes (FTA) on multiple time scales induced by different precipitation are investigatedUncertainties in climatology and interannual variability of global total FTA are about 41% of uncertainties in TRENDYv6 simulationsA good linear relationship emerges between global area‐averaged land climatological annual precipitation and simulated total FTA [ABSTRACT FROM AUTHOR]

Indian Ocean Dipole (IOD), a major climate variability in the tropics which drives the abiotic stress associated with heavy rainfalls and severe droughts, is not much understood in terms of its role in the carbon cycle, while El Niño‐Southern Oscillation (ENSO)‐related terrestrial carbon cycle variation has been intensively studied. Here, we investigate IOD's impact on land photosynthesis over the Indian ocean rim countries during austral spring using satellite‐based gross primary productivity (GPP) and Earth System Model simulations produced in the Coupled Model Intercomparison Project Phase 6 (CMIP6). IOD independently affects GPP with significant positive partial correlation coefficients (pcor) over most of Africa and India, and negative pcor over southern China, Indo‐China peninsula, maritime continent, and Australia, mostly driven by precipitation variations; this obviously differs from the widespread significant negative pcor pattern induced by ENSO. The recent extremely positive IOD in 2019 caused the canonical IOD‐affected GPP patterns, however, with its extreme impacts. Furthermore, though large inter‐model spreads exist, the CMIP6 multimodel median can basically capture the main characteristics of IOD‐affected precipitation and GPP patterns. Importantly, IOD is predicted to occur more frequently in future warming scenarios. Model future projections suggest that it will exert larger impacts on GPP variations over central and eastern Africa, Sumatra, western and southeastern Australia with stronger pcor and enhanced explained variance, but less impacts over southern Africa, east India, Indo‐China peninsula, and northeastern Australia. Therefore, besides ENSO, understanding the IOD impacts can provide us new insights into regional and global carbon cycle interannual variability. Plain Language Summary: Indian ocean dipole (IOD) is a major climate variability in the tropics, acting as an important determinant of regional climate variations. However, its role in the carbon cycle received less attention. Here we explore the impact of IOD events on land photosynthesis over the Indian ocean rim countries during austral spring based on satellite‐based gross primary productivity (GPP) and historical and future simulations in 10 Earth System models. We suggest that IOD can positively impact on GPP over most of Africa and India, but negatively impact over southern China, Indo‐China peninsula, maritime continent, and Australia, which are mostly driven by heavy rainfalls and severe droughts associated with its two sea surface temperature poles. The recent extremely positive IOD in 2019 resulted in the canonical but extreme IOD‐affected GPP patterns. Furthermore, multimodel future projections suggest that the occurrence of IOD events will be more frequent in future warming scenario. IOD will exert larger impacts on GPP variations over the central and eastern Africa, Sumatra, western and southeastern Australia, but lower impacts over southern Africa, east India, the Indo‐China peninsula, and northeastern Australia. Understanding the IOD impacts can provide us new insights into regional and global carbon cycle interannual variability. Key Points: Indian Ocean dipole can exert contrasting impacts on gross primary productivity (GPP) over Indian Ocean rim countries driven by precipitation variationsThe recent extremely positive Indian Ocean Dipole (IOD) in 2019 caused the canonical but extreme IOD‐affected GPP patternsMultimodel future projections suggest that IOD will occur more frequently with larger impacts on GPP, especially over eastern Africa [ABSTRACT FROM AUTHOR]

Knowledge of trophic ecology of fish species is fundamental to understand the organization mechanism of fish assemblages and crucial for developing effective management and conservation strategies. This study examined the diet partitioning and trophic guild structure of fish assemblages in Chishui River, the last undammed tributary of the upper Yangtze River, China. Specimens were collected in three sections along the upstream–downstream gradient in 2012. Digestive tract contents of 54 fish species, represented by 692 individuals, were analysed and quantified. Analysis showed that fish assemblages in the Chishui River utilized aquatic insects, algae, detritus, molluscs, and fishes as the main food resources, whereas terrestrial insects were consumed only occasionally. The trophic guild structure of fish assemblages changed gradually with the longitudinal gradient. Specially, the number of trophic guilds increased with downstream distance. In addition, the relative number of omnivorous species increased longitudinally, whereas the relative number of insectivorous species declined downstream. This study demonstrated how fish assemblages utilize the food resources under near‐natural conditions. These results provide valuable information for fish conservation and ecological restoration. [ABSTRACT FROM AUTHOR]

Summary: A coexpression network is a powerful tool for revealing genes' relationship with many biological processes. Mass transcriptomic and genomic data from different plant species provide the foundation for understanding the evolution of nodulation across the Viridiplantae at a systematic level.We used weighted coexpression network analysis (WGCNA) to mine a nodule‐related module (NRM) in Glycine max.Comparative genomic analysis of 78 green plant species revealed that NRM genes are recruited from different evolutionary nodes along with gene duplication events. A set of core coexpressed genes within legumes may play vital roles in regulating nodule environments essential for nitrogen fixation, including oxygen concentrations, sulfur transport, and iron homeostasis (such as GmCHY). The regulation of these genes occurred mainly at the transcription level, although some of them, such as sulfate transporters, may also undergo positive selection at protein level.We revealed that ancient orthologs and duplication events before the origin of legumes were preadapted for symbiosis. Conserved coregulated genes found within legumes paved the way for nodule formation and nitrogen fixation. These findings provide significant insights into the evolution of nodulation and indicate promising candidates for identifying other key components of legume nodulation and nitrogen fixation. [ABSTRACT FROM AUTHOR]

Abstract: The intraseasonal variation of the Tibetan Plateau summer monsoon (TPSM) during 1979–2011 is investigated. The TPSM shows a dominant quasi‐biweekly oscillation (QBWO) in most summer seasons, and its active/break phases are closely related to more/less precipitation over the Tibetan Plateau. We suggest that the TPSM QBWO is associated with a southeastward propagating nonstationary wave train in the middle and upper troposphere. It shows equivalent barotropic vertical structures over the midlatitudes and a baroclinic structure over the eastern Tibetan Plateau. Wave activity flux analysis indicates that it originates from northern Europe, which is an active center of the summertime Arctic Oscillation (AO). The AO also shows significant QBWO signals and leads TPSM QBWO by about 13 days. Phase composite and wave activity flux analyses of AO QBWO confirmed that the wave train influences TPSM QBWO, suggesting that AO plays an important role in the TPSM on a 10‐ to 20‐day timescale. [ABSTRACT FROM AUTHOR]

Summary: WRKY transcription factors are known to participate in the defence responses of higher plants. However, little is known about the roles of such proteins, especially regarding their functions in the resistance of oilseed rape (Brassica napus) to Sclerotinia sclerotiorum, a necrotrophic fungal pathogen that causes stem rot. In this study, we identified BnWRKY33 as a S. sclerotiorum‐responsive gene that positively regulates resistance to this pathogen by enhancing the expression of genes involved in camalexin synthesis and genes regulated by salicylic acid (SA) and jasmonic acid (JA). We also identified a S. sclerotiorum‐responsive region in the promoter of BnWRKY33, which we revealed to be a relatively conserved W‐box region in the promoters of homologous genes in different species. Using this S. sclerotiorum‐responsive region as bait in a yeast one‐hybrid assay, we identified another WRKY transcription factor, BnWRKY15, and observed that both BnWRKY15 and BnWRKY33 could bind to this region. In addition, BnWRKY15 overexpression simultaneously increased the susceptibility of B. napus to S. sclerotiorum and down‐regulated BnWRKY33 after different durations of infection. Furthermore, BnWRKY15, which contains a transcriptional repression domain, exhibited reduced transactivation ability and could reduce the transactivation ability of BnWRKY33 in Arabidopsis protoplast assays. Therefore, we suggest that the increased susceptibility of BnWRKY15‐overexpressing plants results from reduced BnWRKY33 expression, which is due to the inhibition of BnWRKY33 transcriptional activation by BnWRKY15. [ABSTRACT FROM AUTHOR]

A facile oxidative annulation of cyclohexanones and 2-aminophenyl ketones that uses molecular oxygen as the sole oxidant is described. The reaction provides a direct approach to acridines, a structural motif for a large number of fluorescent sensors, functional materials, ligands, and pharmaceuticals. In the presence of a palladium catalyst, high regioselectivity is observed when using non-symmetric 3-substituted cyclohexanones. With the use of oxygen as the terminal redox moderator, the electron gap of the global redox condensation process is filled and the reaction efficiency is significantly promoted. This protocol possesses many advantages such as using non-hazardous oxidant and readily available starting materials, high regioselectivity, and water as the only by-product. [ABSTRACT FROM AUTHOR]

A facile spinning-based strategy was developed to fabricate chitosan (CS) surface nanofiber-based affinity membranes for protein adsorption. The core-shell nanofiber mat of nylon 6-CS was prepared via coaxial solution blowing process. The nanofibers have a diameter range of 60-300 nm. The core-shell structure was confirmed by transmission electron microscopy, and CS was observed as a thin layer that uniformly adhered to the core. The dye ligand of cibacron blue F3GA (CB F3GA) was further covalently immobilized on the nanofibers with a content of 425 µmol/g. The pristine and CB F3GA-attached mats were studied in protein adsorption. High bovine serum albumin adsorption capacities of 91.9 and 219.6 mg/g were obtained for pristine and CB F3GA-attached mats, respectively. Given its properties of high flux rate and low pressure drop, CB F3GA-attached nylon 6-CS nanofiber mat meets the requirements of highly effective affinity membrane chromatography. Copyright © 2016 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]

ABSTRACT Multimode fiber Bragg grating (MFBG) is inscribed in graded-index multimode fiber using infrared femtosecond laser and phase mask. Multiple dips are observed in the transmission spectrum of MFBG. There is not only self-coupling effect between the forward and backward principal modes, but also the mutual coupling effect between the neighbor modes. The induced refractive index modulation of 6.4 × 10 −4 is achieved for a peak power density of 4.1 × 1013 W/cm2 without any fiber sensitization. Thermal experiment results show that the MFBG belongs to type I grating. The transmission spectrum of MFBG shifts linearly with the strain and pressure at sensitivities of 1.27 ± 0.002 pm/με and −3.3 ± 0.06 pm/MPa, respectively. The MFBGs are expected to be used for multichannel filters, multiwavelength fiber lasers, and multiparameter sensing. © 2016 Wiley Periodicals, Inc. Microwave Opt Technol Lett 59:214-219, 2017 [ABSTRACT FROM AUTHOR]

A direct sulfination of nonactivated alcohols with arylsulfonylmethyl isocyanides under mild conditions [10 mol-% of Bi(OTf)3, 25 °C] was developed to afford sulfinates instead of the usually favored sulfones. The in situ multiple alcohol activation strategies, along with the properties of sulfones and sulfinate anions, were proposed to explain the reversed sulfinate/sulfone selectivity. [ABSTRACT FROM AUTHOR]

Promotion of myofibroblast apoptosis is a potential therapeutic strategy for pulmonary fibrosis. This study investigated the antifibrotic effect of astaxanthin on the promotion of myofibroblast apoptosis based on dynamin-related protein-1 (Drp1)-mediated mitochondrial fission in vivo and in vitro. Results showed that astaxanthin can inhibit lung parenchymal distortion and collagen deposition, as well as promote myofibroblast apoptosis. Astaxanthin demonstrated pro-apoptotic function in myofibroblasts by contributing to mitochondrial fission, thereby leading to apoptosis by increasing the Drp1 expression and enhancing Drp1 translocation into the mitochondria. Two specific si RNAs were used to demonstrate that Drp1 is necessary to promote astaxanthin-induced mitochondrial fission and apoptosis in myofibroblasts. Drp1-associated genes, such as Bcl-2-associated X protein, cytochrome c, tumour suppressor gene p53 and p53-up-regulated modulator of apoptosis, were highly up-regulated in the astaxanthin group compared with those in the sham group. This study revealed that astaxanthin can prevent pulmonary fibrosis by promoting myofibroblast apoptosis through a Drp1-dependent molecular pathway. Furthermore, astaxanthin provides a potential therapeutic value in pulmonary fibrosis treatment. [ABSTRACT FROM AUTHOR]

In the past decades, it has been debated whether ecological niche should be conserved among closely related species (phylogenetic niche conservatism, PNC) or largely divergent (traditional ecological niche theory and ecological speciation) and whether niche specialist and generalist might remain in equilibrium or niche generalist could not appear. In this study, we employed morphological traits to describe ecological niche and test whether different niche dimensions exhibit disparate evolutionary patterns. We conducted our analysis on three Rhinogobio fish species ( R. typus, R. cylindricus, and R. ventralis) from the upper Yangtze River, China. Among the 32 measured morphological traits except body length, PCA extracted the first four principal components with their loading scores >1.000. To find the PNC among species, Mantel tests were conducted with the Euclidean distances calculated from the four principal components (representing different niche dimensions) against the pairwise distances calculated from mitochondrial cytochrome b sequence variations. The results showed that the second and the third niche dimension, both related to swimming ability and behavior, exhibited phylogenetic conservatism. Further comparison on niche breadth among these three species revealed that the fourth dimension of R. typus showed the greatest width, indicating that this dimension exhibited niche generalism. In conclusion, our results suggested that different niche dimensions could show different evolutionary dynamic patterns: they may exhibit PNC or not, and some dimensions may evolve generalism. [ABSTRACT FROM AUTHOR]

Apoptosis of type II alveolar epithelial cells ( AECs-II) is a key determinant of initiation and progression of lung fibrosis. However, the mechanism of miR-30a participation in the regulation of AECs-II apoptosis is ambiguous. In this study, we investigated whether miR-30a could block AECs-II apoptosis by repressing mitochondrial fission dependent on dynamin-related protein-1 (Drp-1). The levels of miR-30a in vivo and in vitro were determined through quantitative real-time PCR ( qRT- PCR). The inhibition of miR-30a in AECs-II apoptosis, mitochondrial fission and its dependence on Drp-1, and Drp-1 expression and translocation were detected using miR-30a mimic, inhibitor-transfection method (gain- and loss-of-function), or Drp-1 si RNA technology. Results showed that miR-30a decreased in lung fibrosis. Gain- and loss-of-function studies revealed that the up-regulation of miR-30a could decrease AECs-II apoptosis, inhibit mitochondrial fission, and reduce Drp-1 expression and translocation. MiR-30a mimic/inhibitor and Drp-1 si RNA co-transfection showed that miR-30a could inhibit the mitochondrial fission dependent on Drp-1. This study demonstrated that miR-30a inhibited AECs-II apoptosis by repressing the mitochondrial fission dependent on Drp-1, and could function as a novel therapeutic target for lung fibrosis. [ABSTRACT FROM AUTHOR]

Long non-coding RNAs (lnc RNAs) are involved in various pathophysiologic processes and human diseases. However, their dynamics and corresponding functions in pulmonary fibrosis remain poorly understood. In this study, portions of lnc RNAs adjacent or homologous to protein-coding genes were determined by searching the UCSC genome bioinformatics database. This was found to be potentially useful for exploring lnc RNA functions in disease progression. Previous studies showed that competing endogenous RNA (ce RNA) hypothesis is another method to predict lnc RNA function. However, little is known about the function of ce RNA in pulmonary fibrosis. In this study, we selected two differentially expressed lnc RNAs MRAK088388 and MRAK081523 to explore their regulatory mechanisms. MRAK088388 and MRAK081523 were analysed as long-intergenic non-coding RNAs (linc RNAs), and identified as orthologues of mouse lnc RNAs AK088388 and AK081523, respectively. q RT- PCR and in situ hybridization (ISH) showed that they were significantly up-regulated, and located in the cytoplasm of interstitial lung cells. We also showed that MRAK088388 and N4bp2 had the same mi RNA response elements ( MREs) for miR-200, miR-429, miR-29, and miR-30, whereas MRAK081523 and Plxna4 had the same MREs for miR-218, miR-141, miR-98, and let-7. Moreover, the expression levels of N4bp2 and Plxna4 significantly increased in fibrotic rats, and were highly correlated with those of MRAK088388 and MRAK081523, respectively. Among their shared mi RNAs, miR-29b-3p and let-7i-5p decreased in the model group, and were negatively correlated with the expression of MRAK088388 and MRAK081523, respectively. MRAK088388 and MRAK081523 could regulate N4bp2 and Plxna4 expression by sponging miR-29b-3p and let-7i-5p, respectively, and possessed regulatory functions as ce RNAs. Thus, our study may provide insights into the functional interactions of lnc RNA, mi RNA and m RNA, and lead to new theories for the pathogenesis and treatment of pulmonary fibrosis. [ABSTRACT FROM AUTHOR]

The aim of the study was to investigate correlations between intrahepatic hepatitis B virus total DNA, covalently closed circular DNA (cccDNA), and serum HBsAg in treatment-naïve chronic hepatitis B and HBV related hepatocellular carcinoma (HCC). Liver tissues were taken from 42 HBV related HCC and 36 patients with chronic hepatitis B. A fraction of DNA extracted from liver tissue was digested with a plasmid-safe ATP-dependent DNase and used for HBV cccDNA detection. The remaining DNA was used for the detection of HBV total DNA and β-globin, the latter of which is a housekeeping gene and quantified for normalization by real-time PCR. Quantitation of serum HBsAg was performed by a chemiluminescence assay. Serum HBsAg had positive correlations with serum HBV DNA (r = 0.636, P < 0.001), intrahepatic HBV total DNA (r = 0.519, P = 0.001) and cccDNA (r = 0.733, P < 0.001) in 36 treatment-naïve chronic hepatitis B, while HBsAg correlated poorly with DNA (r = 0.224, P = 0.210), intrahepatic total DNA and cccDNA in the tumor (r = 0.351, P = 0.031; r = 0.164, P = 0.324, respectively) and non-tumor (r = 0.237, P = 0.152; r = 0.072, P = 0.667, respectively) liver tissues of 42 HCC. HBV cccDNA and total DNA were significantly higher in liver tissue from chronic hepatitis B than in tumor and non-tumor of HCC ( P < 0.001). Serum HBsAg and HBV DNA were also higher in chronic hepatitis B than in HCC ( P < 0.001). It was concluded that levels of serum HBsAg and intrahepatic cccDNA and total DNA were significantly higher in chronic hepatitis B than in HCC, and significant correlations among them were observed in treatment-naïve chronic hepatitis B but not in HCC. J. Med. Virol. 85:219-227, 2013. © 2012 Wiley Periodicals, Inc. [ABSTRACT FROM AUTHOR]

An abstract of the article "Efficient Synthesis of Isoquinolines by AgNO3-Catalyzed Sequential Imination-Annulation of 2-Alkynyl Aldehydes with Ammonium Bicarbonate" by Y. Zhao and colleagues is presented.

Apoptosis of type II alveolar epithelial cells (AECs-II) is a key determinant of initiation and progression of lung fibrosis. However, the mechanism of miR-30a participation in the regulation of AECs-II apoptosis is ambiguous. In this study, we investigated whether miR-30a could block AECs-II apoptosis by repressing mitochondrial fission dependent on dynamin-related protein-1 (Drp-1). The levels of miR-30a in vivo and in vitro were determined through quantitative real-time PCR (qRT-PCR). The inhibition of miR-30a in AECs-II apoptosis, mitochondrial fission and its dependence on Drp-1, and Drp-1 expression and translocation were detected using miR-30a mimic, inhibitor-transfection method (gain- and loss-of-function), or Drp-1 siRNA technology. Results showed that miR-30a decreased in lung fibrosis. Gain- and loss-of-function studies revealed that the up-regulation of miR-30a could decrease AECs-II apoptosis, inhibit mitochondrial fission, and reduce Drp-1 expression and translocation. MiR-30a mimic/inhibitor and Drp-1 siRNA co-transfection showed that miR-30a could inhibit the mitochondrial fission dependent on Drp-1. This study demonstrated that miR-30a inhibited AECs-II apoptosis by repressing the mitochondrial fission dependent on Drp-1, and could function as a novel therapeutic target for lung fibrosis., (© 2014 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.)

Long non-coding RNAs (lncRNAs) are involved in various pathophysiologic processes and human diseases. However, their dynamics and corresponding functions in pulmonary fibrosis remain poorly understood. In this study, portions of lncRNAs adjacent or homologous to protein-coding genes were determined by searching the UCSC genome bioinformatics database. This was found to be potentially useful for exploring lncRNA functions in disease progression. Previous studies showed that competing endogenous RNA (ceRNA) hypothesis is another method to predict lncRNA function. However, little is known about the function of ceRNA in pulmonary fibrosis. In this study, we selected two differentially expressed lncRNAs MRAK088388 and MRAK081523 to explore their regulatory mechanisms. MRAK088388 and MRAK081523 were analysed as long-intergenic non-coding RNAs (lincRNAs), and identified as orthologues of mouse lncRNAs AK088388 and AK081523, respectively. qRT-PCR and in situ hybridization (ISH) showed that they were significantly up-regulated, and located in the cytoplasm of interstitial lung cells. We also showed that MRAK088388 and N4bp2 had the same miRNA response elements (MREs) for miR-200, miR-429, miR-29, and miR-30, whereas MRAK081523 and Plxna4 had the same MREs for miR-218, miR-141, miR-98, and let-7. Moreover, the expression levels of N4bp2 and Plxna4 significantly increased in fibrotic rats, and were highly correlated with those of MRAK088388 and MRAK081523, respectively. Among their shared miRNAs, miR-29b-3p and let-7i-5p decreased in the model group, and were negatively correlated with the expression of MRAK088388 and MRAK081523, respectively. MRAK088388 and MRAK081523 could regulate N4bp2 and Plxna4 expression by sponging miR-29b-3p and let-7i-5p, respectively, and possessed regulatory functions as ceRNAs. Thus, our study may provide insights into the functional interactions of lncRNA, miRNA and mRNA, and lead to new theories for the pathogenesis and treatment of pulmonary fibrosis., (© 2014 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.)