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Cellular senescence is acknowledged as a key contributor to organismal ageing and late‐life disease. Though popular, the study of senescence in vitro can be complicated by the prolonged and asynchronous timing of cells committing to it and by its paracrine effects. To address these issues, we repurposed a small molecule inhibitor, inflachromene (ICM), to induce senescence to human primary cells. Within 6 days of treatment with ICM, senescence hallmarks, including the nuclear eviction of HMGB1 and ‐B2, are uniformly induced across IMR90 cell populations. By generating and comparing various high throughput datasets from ICM‐induced and replicative senescence, we uncovered a high similarity of the two states. Notably though, ICM suppresses the pro‐inflammatory secretome associated with senescence, thus alleviating most paracrine effects. In summary, ICM rapidly and synchronously induces a senescent‐like phenotype thereby allowing the study of its core regulatory program without confounding heterogeneity. [ABSTRACT FROM AUTHOR]

Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is a rare familial neurological disorder caused by mutations in the NOTCH3 gene and characterized by migraine attacks, depressive episodes, lacunar strokes, dementia, and premature death. Since there is no therapy for CADASIL the authors investigate whether the multi‐modal neuropeptide drug Cerebrolysin may improve outcome in a murine CADASIL model. Twelve‐month‐old NOTCH3R169C mutant mice (n=176) are treated for nine weeks with Cerebrolysin or Vehicle and histopathological and functional outcomes are evaluated within the subsequent ten months. Cerebrolysin treatment improves spatial memory and overall health, reduces epigenetic aging, and prolongs lifespan, however, CADASIL‐specific white matter vacuolization is not affected. On the molecular level Cerebrolysin treatment increases expression of Calcitonin Gene‐Related Peptide (CGRP) and Silent Information Regulator Two (Sir2)‐like protein 6 (SIRT6), decreases expression of Insulin‐like Growth Factor 1 (IGF‐1), and normalizes the expression of neurovascular laminin. In summary, Cerebrolysin fosters longevity and healthy aging without specifically affecting CADASIL pathology. Hence, Cerebrolysin may serve a therapeutic option for CADASIL and other disorders characterized by accelerated aging. [ABSTRACT FROM AUTHOR]

Rejuvenation of cells by reprogramming toward the pluripotent state raises increasing attention. In fact, generation of induced pluripotent stem cells (iPSCs) completely reverses age‐associated molecular features, including elongation of telomeres, resetting of epigenetic clocks and age‐associated transcriptomic changes, and even evasion of replicative senescence. However, reprogramming into iPSCs also entails complete de‐differentiation with loss of cellular identity, as well as the risk of teratoma formation in anti‐ageing treatment paradigms. Recent studies indicate that partial reprogramming by limited exposure to reprogramming factors can reset epigenetic ageing clocks while maintaining cellular identity. So far, there is no commonly accepted definition of partial reprogramming, which is alternatively called interrupted reprogramming, and it remains to be elucidated how the process can be controlled and if it resembles a stable intermediate state. In this review, we discuss if the rejuvenation program can be uncoupled from the pluripotency program or if ageing and cell fate determination are inextricably linked. Alternative rejuvenation approaches with reprogramming into a pluripotent state, partial reprogramming, transdifferentiation, and the possibility of selective resetting of cellular clocks are also discussed. [ABSTRACT FROM AUTHOR]

Understanding the role of soil moisture and other controls in runoff generation is important for predicting runoff across scales. This paper aims to identify the degree of non‐linearity of the relationship between event peak runoff and potential controls for different runoff generation mechanisms in a small agricultural catchment. The study is set in the 66 ha Hydrological Open Air Laboratory, Austria, where discharge was measured at the catchment outlet and for 11 sub‐catchments or hillslopes with different runoff generation mechanisms. Peak runoff of 73 events was related to three potential controls: event precipitation, soil moisture and groundwater levels. The results suggest that the hillslopes dominated by ephemeral overland flow exhibit the most non‐linear runoff generation behaviour for its controls; runoff is only generated above a threshold of 95% of the maximum soil moisture. Runoff generation through tile drains and in wetlands is more linear. The largest winter and spring events at the catchment outlet are caused by runoff from hillslopes with shallow flow paths (ephemeral overland flow and tile drainage mechanisms), while the largest summer events are caused by other hillslopes, those with deeper flow paths or with saturation areas throughout the year. Therefore, the response of the entire catchment is a mix of the various mechanisms, and the groundwater contribution makes the response more linear. The implications for hydrological modelling are discussed. [ABSTRACT FROM AUTHOR]

It has already been proposed that a combined use of different molecular and morphological markers of aging in multivariate models may result in a greater accuracy of age estimation. However, such an approach can be complex and expensive, and not every combination may be useful. The significance and usefulness of combined analyses of D‐aspartic acid in dentine, pentosidine in dentine, DNA methylation in buccal swabs at five genomic regions (PDE4C, RPA2, ELOVL2, DDO, and EDARADD), and third molar mineralization were tested by investigating a sample of 90 oral surgery patients. Machine learning models for age estimation were trained and evaluated, and the contribution of each parameter to multivariate models was tested by assessment of the predictor importance. For models based on D‐aspartic acid, pentosidine, and the combination of both, mean absolute errors (MAEs) of 2.93, 3.41, and 2.68 years were calculated, respectively. The additional inclusion of the five DNAm markers did not improve the results. The sole DNAm‐based model revealed a MAE of 4.14 years. In individuals under 28 years of age, the combination of the DNAm markers with the third molar mineralization stages reduced the MAE from 3.85 to 2.81 years. Our findings confirm that the combination of parameters in multivariate models may be very useful for age estimation. However, the inclusion of many parameters does not necessarily lead to better results. It is a task for future research to identify the best selection of parameters for the different requirements in forensic practice. [ABSTRACT FROM AUTHOR]

This analysis departs from social identity being part and parcel of the system of social representations a group holds. Additionally, social identity is seen as being affectively laden with religious or other deeply ingrained ideological beliefs. These systems delimit the space of norms and behaviours where people socially construct the objects defining the local world as an objectively given. The local objectifications differ between groups and cultures and pose a problem for interaction and communication between members of different groups. Inter‐group discrepancies must be bridged by establishing a shared representational space and interobjective understanding. This understanding can be reached if both interactants mutually develop meta‐representations that allow them to grossly anticipate what to expect from the other and to orient their actions accordingly. But there is a catch in establishing interobjectivity: even if the interactants manage to overcome semantic and cognitive barriers, their interaction and open‐minded communication will be blocked if the two objectivities are ethically or affectively incommensurable. I illustrate this process by suggesting that native Europeans will feel blocked to establish interobjective acceptance for example with proponents of a slave holder culture whose values are incommensurable with European ideas of human equality. Accepting a slave holder's worldview would threaten the European's identity. A similar process seems to govern devout conservative Muslims living in 'gated communities' and enclaves in European cities who try to maintain their identity by avoiding regular contact with the non‐Muslim majority. Such contact would threaten their identity if their worldview and sharia rules were measured by the standards of human rights. Native Europeans on the other hand, will feel their own cultural objectivity to be incompatible with traditional Islam that does not equally observe human rights. The implications of such processes are discussed in terms of migrants' integration, multiculturalism, and socio‐political psychology. [ABSTRACT FROM AUTHOR]

Recent rise of populist parties has brought about more contestation over development policy. There is also increasing diversity within the European Union: some countries allocate more development aid while the contributions of newer member states are on average smaller. But do such national interests surface more often than in other issue areas, and what is the structure of contestation over development policy in the European Parliament (EP)? Examining roll‐call votes and the processing of the 2017 European Consensus on Development, this article shows that opposition to development policy is restricted to the more Eurosceptical representatives. EP party groups attain similar levels of cohesion in votes on development aid as in other policy areas, with coalition patterns following the left–right dimension. Development policy is thus 'business as usual' in the Parliament, but the results suggest increasing politicization of aid through stronger horizontal linkages between immigration, security, and development policy. [ABSTRACT FROM AUTHOR]

The awareness of drought and its impacts on Central Europe increased after the significant drought episodes in 2000, 2003, 2012 and 2015, which were all estimated to have caused over 500 million Euro in damage in the Czech Republic alone. These events indicated the need for timely and high‐resolution monitoring tools that would enable analysing, monitoring and forecasting of drought events. Monitoring soil water availability in near real time and at high‐resolution (up to 0.5 × 0.5 km for some products) helps farmers and water managers to mitigate impacts of these extreme events. The Czech Drought Monitor was developed between 2012 and 2014 and has since been operational as an online platform. It uses an operational modelling system that consists of four pillars: (a) weekly soil moisture estimates based on spaceborne Advanced Scatterometer sensor measurements; (b) the daily SoilClim soil moisture model, which runs based on high‐density network data from the Czech Hydrometeorological Institute with a 55‐year reference period; (c) weekly reports on vegetation condition, which is deduced from satellite‐based vegetation indices and early warnings of imminent drought impacts; and (d) weekly reports of soil moisture, especially after drought impacts, which are provided by dozens of experts. Since 2016 the drought forecast (+9 days) has been released daily based on an ensemble of five numerical weather prediction models combined with a weekly drought outlook (+2 months). The analysis of four recent episodes (2000, 2003, 2012 and 2015) clearly showed that both large‐scale and regionally restrained drought episodes posed serious risks in terms of their impacts and damage. Comparisons with historical droughts showed that these events, especially the 2000, 2003 and 2015 events, were among the top five drought episodes in the June–August period observed in the Czech Republic since 1961 in terms of spatial extent, magnitude and duration. [ABSTRACT FROM AUTHOR]

Cdc42 is a small RhoGTPase regulating multiple functions in eukaryotic cells. The activity of Cdc42 is significantly elevated in several tissues of aged mice, while the Cdc42 gain‐of‐activity mouse model presents with a premature aging‐like phenotype and with decreased lifespan. These data suggest a causal connection between elevated activity of Cdc42, aging, and reduced lifespan. Here, we demonstrate that systemic treatment of aged (75‐week‐old) female C57BL/6 mice with a Cdc42 activity‐specific inhibitor (CASIN) for 4 consecutive days significantly extends average and maximum lifespan. Moreover, aged CASIN‐treated animals displayed a youthful level of the aging‐associated cytokines IL‐1β, IL‐1α, and INFγ in serum and a significantly younger epigenetic clock as based on DNA methylation levels in blood cells. Overall, our data show that systemic administration of CASIN to reduce Cdc42 activity in aged mice extends murine lifespan. [ABSTRACT FROM AUTHOR]

Since 1965, in Indonesia, people labelled as communists and their descendants have been mistreated. Recently, there has been an issue to apologize to them, but up until now, no official apology has been offered by the Indonesian government. The present study aims to understand how communism is perceived in Indonesia and why communism attributes labelled to a person can lead to negative effects, such as social exclusions. We interviewed 23 Muslims living in Jakarta (i.e., majority group) and used a thematic analysis to organize and describe the data. We found that Muslims who see communists as non‐believers view communist members as dangerous and a threat for the unity of the Republic of Indonesia. Among participants with such beliefs, the past maltreatments to accused communists are justified and legitimate; any apology toward the victims is considered not needed. The findings are discussed in the context of collective blaming and group essentialization, and ways to solve the problems are suggested. [ABSTRACT FROM AUTHOR]

Based on our studies on biphasic self‐assembly behavior of an amphiphilic BF2‐azadipyrromethene (aza‐BODIPY) dye 1, a new analytical model to quantitatively describe the thermodynamic properties of the aggregation involving two competing supramolecular polymerization processes is proposed. In this model, the formation of the metastable as well as the thermodynamically stable aggregates was considered to follow a nucleated polymerization mechanism. The numerical calculation based on the new model gives insight into the formation of different species in such complicate aggregate systems. Moreover, the aggregation of the biphasic self‐assembly processes for dye 1 was investigated by concentration‐dependent UV/Vis spectroscopy. The experimental data were analyzed by using the new model to evaluate the thermodynamic parameters including aggregation constants, the size of nuclei, and the cooperativity the two types of aggregates. Modelling polymerization: A new analytical model of coupled cooperative supramolecular polymerizations is proposed to describe the biphasic aggregation of an amphiphilic BF2‐azadipyrromethene (BODIPY) dye. [ABSTRACT FROM AUTHOR]

Inequalities between men and women are common and well‐documented. Objective indexes show that men are better positioned than women in societal hierarchies—there is no single country in the world without a gender gap. In contrast, researchers have found that the women‐are‐wonderful effect—that women are evaluated more positively than men overall—is also common. Cross‐cultural studies on gender equality reveal that the more gender egalitarian the society is, the less prevalent explicit gender stereotypes are. Yet, because self‐reported gender stereotypes may differ from implicit attitudes towards each gender, we reanalysed data collected across 44 cultures, and (a) confirmed that societal gender egalitarianism reduces the women‐are‐wonderful effect when it is measured more implicitly (i.e. rating the personality of men and women presented in images) and (b) documented that the social perception of men benefits more from gender egalitarianism than that of women. [ABSTRACT FROM AUTHOR]

The “free radical theory of aging” suggests that reactive oxygen species (ROS) are responsible for age‐related loss of cellular functions and, therefore, represent the main cause of aging. Redox regulation by thioredoxin‐1 (TRX) plays a crucial role in responses to oxidative stress. We show that thioredoxin‐interacting protein (TXNIP), a negative regulator of TRX, plays a major role in maintaining the redox status and, thereby, influences aging processes. This role of TXNIP is conserved from flies to humans. Age‐dependent upregulation of TXNIP results in decreased stress resistance to oxidative challenge in primary human cells and in Drosophila. Experimental overexpression of TXNIP in flies shortens lifespan due to elevated oxidative DNA damage, whereas downregulation of TXNIP enhances oxidative stress resistance and extends lifespan. [ABSTRACT FROM AUTHOR]

ABSTRACT: Osteoporosis is an age‐related metabolic bone disease. Hence, osteoporotic patients might suffer from molecular features of accelerated aging, which is generally reflected by specific age‐associated DNA methylation (DNAm) changes. In this study, we analyzed genomewide DNAm profiles of peripheral blood from patients with manifest primary osteoporosis and non‐osteoporotic controls. Statistical analysis did not reveal any individual CG dinucleotides (CpG sites) with significant aberrant DNAm in osteoporosis. Subsequently, we analyzed if age‐associated DNAm patterns are increased in primary osteoporosis (OP). Using three independent age‐predictors we did not find any evidence for accelerated epigenetic age in blood of osteoporotic patients. Taken together, osteoporosis is not reflected by characteristic DNAm patterns of peripheral blood that might be used as biomarker for the disease. The prevalence of osteoporosis is age‐associated—but it is not associated with premature epigenetic aging in peripheral blood. © 2017 American Society for Bone and Mineral Research. [ABSTRACT FROM AUTHOR]

The self-assembly of a new perylene bisimide (PBI) organogelator with 1,7-dimethoxy substituents in the bay position affords non-fluorescent H-aggregates at high cooling rates and fluorescent J-aggregates at low cooling rates. Under properly adjusted conditions, the kinetically trapped 'off-pathway' H-aggregates are transformed into the thermodynamically favored J-aggregates, a process that can be accelerated by the addition of J-aggregate seeds. Spectroscopic studies revealed a subtle interplay of π-π interactions and intra- and intermolecular hydrogen bonding for monomeric, H-, and J-aggregated PBIs. Multiple polymerization cycles initiated from the seed termini demonstrate the living character of this chain-growth supramolecular polymerization process. [ABSTRACT FROM AUTHOR]

The European Union is best understood as a liberal power - as an actor that is composed of liberal democracies whose interests, identities and institutions motivate and constrain its policy. The conceptualization of the EU as a liberal power helps to overcome three shortcomings of alternative notions such as civilian power or Normative Power Europe: First, norms are not only understood as driving forces but also as constraints on foreign policy; second, liberal power Europe emphasizes the contested nature of norms and conflicts between norms and thus draws attention to the politics of EU external relations; third, it encourages an engagement with the vast literature on the distinctive policies of liberal democracies in international relations and foreign policy analysis. The EU's crisis management serves to illustrate the value-added of the liberal power Europe concept. [ABSTRACT FROM AUTHOR]

A new amphiphilic BF2-azadipyrromethene (aza-BODIPY) dye 1 has been synthesized using a CuI-catalyzed 'click' reaction. For this dye, two self-assembly pathways that lead to different type of J-aggregates with distinct near-infrared optical properties have been discovered. The metastable off-pathway product displays a broad, structureless absorption band while the thermodynamically stable on-pathway aggregate exhibits the characteristic spectral features of a J-aggregate, that is, red-shifted intense absorption band with significantly narrowed linewidth. The morphology and structure of the aggregates were studied by atomic force microscopy, transmission and scanning electron microscopy. The aggregation processes of 1 were investigated by temperature- and concentration-dependent UV/Vis spectroscopy and evaluated by models for cooperative self-assembly. [ABSTRACT FROM AUTHOR]

Samples of two hundred forty-five majority Sunny Muslims, 87 Ahmadiyya Muslims, and 145 Christians were used to investigate the determinants and mediators of prejudice in interreligious context in Indonesia. First, the study extends the idea of in-group and out-group metaprejudice; both of which were found to mediate the relationship between perceived quality of intergroup relationship and personal prejudice. Second, we expected that majority members are more likely to reject a minority and that a minority is more likely to more strongly reject another minority than the majority for self-serving reasons. Additionally, the Sunni majority will prejudice and reject the Ahmadiyya minority more than the Christian minority due to the strained religious relation between the two Muslim groups. The hypotheses were confirmed. The findings are discussed in the context of stereotyping, and prejudice dynamics in other intergroup conflicts and ways of coping with such conflict are suggested. [ABSTRACT FROM AUTHOR]

The article comments on the role of behaviours in representation processes with regards to socially constructed objects, reality and objectification. Particular focus is given to the social representation theory. The coping response of children to changes in seating order and relations with the teacher is discussed, as well as interobjectivity, objectification processes, and implication of habitual activities for the social representation theory.

Replicative senescence has a major impact on function and integrity of cell preparations. This process is reflected by continuous DNA methylation ( DNAm) changes at specific CpG dinucleotides in the course of in vitro culture, and such modifications can be used to estimate the state of cellular senescence for quality control of cell preparations. Still, it is unclear how senescence-associated DNAm changes are regulated and whether they occur simultaneously across a cell population. In this study, we analyzed global DNAm profiles of human mesenchymal stem cells ( MSCs) and human umbilical vein endothelial cells ( HUVECs) to demonstrate that senescence-associated DNAm changes are overall similar in these different cell types. Subsequently, an Epigenetic-Senescence-Signature, based on six CpGs, was either analyzed by pyrosequencing or by bar-coded bisulfite amplicon sequencing. There was a good correlation between predicted and real passage numbers in bulk populations of MSCs ( R2 = 0.67) and HUVECs ( R2 = 0.97). However, when we analyzed the Epigenetic-Senescence-Signature in subclones of MSCs, the predictions revealed high variation and they were not related to the adipogenic or osteogenic differentiation potential of the subclones. Notably, in clonally derived subpopulations, the DNAm levels of neighboring CpGs differed extensively, indicating that these genomic regions are not synchronously modified during senescence. Taken together, senescence-associated DNAm changes occur in a highly reproducible manner, but they are not synchronously co-regulated. They rather appear to be acquired stochastically-potentially evoked by other epigenetic modifications. [ABSTRACT FROM AUTHOR]

Aging of the organism is associated with highly reproducible DNA methylation (DNAm) changes, which facilitate estimation of donor age. Cancer is also associated with DNAm changes, which may contribute to disease development. Here, we speculate that age-associated DNAm changes may increase the risk of tumor initiation. Notably, when using epigenetic signatures for age-estimations tumor cells are often predicted to be much older than the chronological age of the patient. We demonstrate that aberrant hypermethylation within the gene DNA methyltransferase 3A ( DNMT3A) - which may contribute to initiation of acute myeloid leukemia (AML) - is particularly observed in AML samples that reveal significantly more age-associated DNAm changes. The functional relevance of age-associated DNAm changes remains to be elucidated, but they occur genome wide, in a highly reproducible manner, and most likely influence chromatin organization - and hence may favor acquisition of aberrant DNAm patterns contributing to cancer in the elderly. [ABSTRACT FROM AUTHOR]

This article explores identity work and acculturation work in the lives of British mixed-heritage children and adults. Children, teenagers, and parents with mixed heritage participated in a community arts project that invited them to deliberate, construct, and reconstruct their cultural identities and cultural relations. We found that acculturation, cultural and raced identities, are constructed through a series of oppositional themes: cultural maintenance versus cultural contact; identity as inclusion versus identity as exclusion; institutionalized ideologies versus agency. The findings point towards an understanding of acculturation as a dynamic, situated, and multifaceted process: acculturation in movement. To investigate this, we argue that acculturation research needs to develop a more dynamic and situated approach to the study of identity, representation, and culture. The article concludes with a discussion on the need for political psychologists to develop methods attuned to the tensions and politics of acculturation that are capable of highlighting the possibilities for resistance and social change. [ABSTRACT FROM AUTHOR]

Summary Replicative senescence has fundamental implications on cell morphology, proliferation, and differentiation potential. Here, we describe a simple method to track long-term culture based on continuous DNA-methylation changes at six specific CpG sites. This epigenetic senescence signature can be used as biomarker for various cell types to predict the state of cellular senescence with regard to the number of passages, population doublings, or days of in vitro culture. [ABSTRACT FROM AUTHOR]

Long-term culture of human mesenchymal stromal cells (MSC) has implications on their proliferation and differentiation potential and we have demonstrated that this is associated with up-regulation of the five microRNAs miR-29c, miR-369-5p, miR-371, miR-499, and let-7f. In this study, we examined the role of these senescence-associated microRNAs for cellular aging and differentiation of MSC. Proliferation was reduced upon transfection with miR-369-5p, miR-371, and miR-499. Adipogenic differentiation was impaired by miR-369-5p whereas it was highly increased by miR-371. This was accompanied by respective gene expression changes of some adipogenic key molecules (adiponectin and fatty acid-binding protein 4 [FABP4]). Furthermore luciferase reporter assay indicated that FABP4 is a direct target of miR-369-5p. Microarray analysis upon adipogenic or osteogenic differentiation revealed down-regulation of several microRNAs albeit miR-369-5p and miR-371 were not affected. Expression of the de novo DNA methyltransferases DNMT3A and DNMT3B was up-regulated by transfection of miR-371 whereas expression of DNMT3A was down-regulated by miR-369-5p. In summary, we identified miR-369-5p and miR-371 as antagonistic up-stream regulators of adipogenic differentiation and this might be indirectly mediated by epigenetic modifications. J. Cell. Physiol. 226: 2226-2234, 2011. © 2010 Wiley-Liss, Inc. [ABSTRACT FROM AUTHOR]

This article presents a theory of the perception of hybrids, resulting from cross-breeding natural animals that pertain to different species and of children parented by couples with a mixed ethnic or racial background. The theory states that natural living beings, including humans, are perceived as possessing a deeply ingrained characteristic that is called 'essence' or 'blood' or 'genes' in everyday discourse and that uniquely determines their category membership. If, by whatever means, the genes or essences of two animals of different species are combined in a hybrid, the two incompatible essences collapse, leaving the hybrid in a state of non-identity and non-belonging. People despise this state and reject the hybrid (Study 1). This devaluation effect holds with cross-kind hybrids and with hybrids that arise from genetically combining animals from incompatible habitats across three cultures: Austria, India and Japan (Study 2). In the social world, groups and ethnic or racial categories frequently are essentialized in an analogue way. When people with an essentialist mindset judge ethnically or racially mixed offspring, they perceive a collapse of ethnic or racial essence and, consequently, denigrate these children, as compared to children from 'pure' in-group or out-group parents (Study 3). The findings are discussed in terms of the widespread 'yuck factor' against genetically modified animals, in terms of the cultural concepts of monstrosity and of racism and prejudice. [ABSTRACT FROM AUTHOR]

Within 2–3 months of in vitro culture-expansion, mesenchymal stromal cells (MSC) undergo replicative senescence characterized by cell enlargement, loss of differentiation potential and ultimate growth arrest. In this study, we have analyzed DNA methylation changes upon long-term culture of MSC by using the HumanMethylation27 BeadChip microarray assessing 27 578 unique CpG sites. Furthermore, we have compared MSC from young and elderly donors. Overall, methylation patterns were maintained throughout both long-term culture and aging but highly significant differences were observed at specific CpG sites. Many of these differences were observed in homeobox genes and genes involved in cell differentiation. Methylation changes were verified by pyrosequencing after bisulfite conversion and compared to gene expression data. Notably, methylation changes in MSC were overlapping in long-term culture and aging in vivo. This supports the notion that replicative senescence and aging represent developmental processes that are regulated by specific epigenetic modifications. [ABSTRACT FROM AUTHOR]

Mesenchymal stromal cells (MSC) have been suggested to provide a suitable cellular environment for in vitro expansion of haematopoietic stem and progenitor cells (HPC) from umbilical cord blood. In this study, we have simultaneously analysed the cell division history and immunophenotypic differentiation of HPC by using cell division tracking with carboxyfluorescein diacetate N-succinimidyl ester (CFSE). Co-culture with MSC greatly enhanced proliferation of human HPC, especially of the more primitive CD34+CD38− fraction. Without co-culture CD34 and CD133 expressions decreased after several cell divisions, whereas CD38 expression was up-regulated after some cell divisions and then diminished in fast proliferating cells. Co-culture with MSC maintained a primitive immunophenotype (CD34+, CD133+ and CD38−) for more population doublings, whereas up-regulation of differentiation markers (CD13, CD45 and CD56) in HPC was delayed to higher numbers of cell divisions. Especially MSC of early cell passages maintained CD34 expression in HPC over more cell divisions, whereas MSC of higher passages further enhanced their proliferation rate. Inhibition of mitogen-activated protein kinase 1 (MAPK1) impaired proliferation and differentiation of HPC, but not maintenance of long-term culture initiating cells. siRNA knockdown of N-cadherin and VCAM1 in feeder layer cells increased the fraction of slow dividing HPC, whereas knockdown of integrin beta 1 (ITGB1) and CD44 impaired their differentiation. In conclusion, MSC support proliferation as well as self-renewal of HPC with primitive immunophenotype. The use of early passages of MSC and genetic manipulation of proteins involved in HPC–MSC interaction might further enhance cord blood expansion on MSC. [ABSTRACT FROM AUTHOR]

We investigated a total of 4997 postings on an extreme right-wing Internet discussion board with regard to the groups and themes mentioned. The most frequently mentioned target groups were Africans, Jews, Muslims, Poles, and Turks; the most prominent themes and contexts were conspiracy, criminality, exploitation, threats to German identity, infiltration, mind control and harassment, procreation, rape, and sex. We analysed in detail postings about Africans/Blacks and Jews, that is target groups that were the most clearly connected to particular themes. The analysis reveals that extreme right-wing discourse essentializes the target groups of Jews and Africans/Blacks and ascribes them immutable group-specific attributes that effectively make them ‘natural kinds’. The group of Jews appears as a kind of their own with super-human powers and influence. Africans and Blacks are despised, firstly because their essential characteristics prohibit them to be categorically mixed with Germans (i.e. to become German by nationality) due to their incompatible essence, and secondly when they procreate with Whites. Such procreation produces ‘bastards’ that are met with disgust. We argue that essentialist thinking about social and ethnic groups explains a good part of their rejection by right-wing followers. Copyright © 2009 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]

Projecting essence onto a social category means to think, talk, and act as if the category were a discrete natural kind and as if its members were all endowed with the same immutable attributes determined by the category's essence. Essentializing may happen implicitly or on purpose in representing ingroups and outgroups. We argue that essentializing is a versatile representational tool (a) that is used to create identity in groups with chosen membership in order to make the group appear as a unitary entity, (b) that outsiders often draw on a group's essentialist self-construal in their judgements about the groups, (c) that judgements about members of forced social categories are often informed by essentialist thinking that easily switches to discrimination and racism, and (d) that under certain historical and political conditions members of social categories and groups may contest their essentialized identity, such as parts of the feminist movement, or that they may attempt to reconstruct an essentialized identity, such as parts of the homosexual movement or the largely defunct European nobility. Besides explicit political and power interests, we see communication processes and language use as a tacit force driving essentialization of social categories. [ABSTRACT FROM AUTHOR]

A current challenge in mesenchymal stem cell (MSC)-based cartilage repair is to solve donor and tissue-dependent variability of MSC cultures and to prevent chondrogenic cells from terminal differentiation like in the growth plate. The aim of this study was to select the best source for MSC which could promise stable cartilage formation in the absence of hypertrophy and ectopic in vivo mineralization. We hypothesized that MSC from synovium are superior to bone marrow- and adipose tissue-derived MSC since they are derived from a joint tissue. MSC were characterized by flow cytometry. MSC pellets were cultured under chondrogenic conditions and differentiation was evaluated by histology, gene expression analysis, and determination of alkaline phosphatase activity (ALP). After chondrogenic induction, pellets were transplanted subcutaneously into SCID mice. MSC from bone marrow, adipose tissue, and synovium revealed similar COL2A1/COL10A1 mRNA levels after chondrogenic induction and were positive for collagen-type-X. Bone marrow-derived and adipose tissue-derived MSC showed significantly higher ALP activity than MSC from synovium. Low ALP-activity before transplantation of pellets correlated with marginal calcification of explants. Surprisingly, non-mineralizing transplants specifically lost their collagen-type II, but not collagen-type I deposition in vivo, or were fully degraded. In conclusion, the lower donor-dependent ALP activation and reduced mineralization of synovium-derived heterotopic transplants did not lead to stable ectopic cartilage as known from articular chondrocytes, but correlated with fibrous dedifferentation or complete degeneration of MSC pellets. This emphasizes that beside appropriate induction of differentiation, locking of MSC in the desired differentiation state is a major challenge for MSC-based repair strategies. J. Cell. Physiol. 219: 219–226, 2009. © 2008 Wiley-Liss, Inc. [ABSTRACT FROM AUTHOR]

Accumulating clinical and experimental evidence indicates that mesenchymal stem cells (MSCs) are promising cell types in the treatment of cardiac dysfunction. They may trigger production of reparative growth factors, replace damaged cells and create an environment that favours endogenous cardiac repair. However, identifying mechanisms which regulate the role of MSCs in cardiac repair is still at work. To achieve the maximal clinical benefits, ex vivo manipulation can further enhance MSC therapeutic potential. This review focuses on the mechanism of MSCs in cardiac repair, with emphasis on ex vivo manipulation. [ABSTRACT FROM AUTHOR]

1. Knowledge about the distribution and types of wetlands is in high demand by ecosystem modellers for full greenhouse gas accounting. The scope of this paper is to demonstrate the suitability of satellite radar data for the delineation of wetlands in the tundra and boreal forest biomes of central Siberia. 2. An area of more than 3 million km² in central Siberia was investigated using satellite data. It covers freshwater ecosystems of the tundra and non-forested peatlands in tundra and boreal forest biomes. The satellite data represent the growing seasons of 2003/2004. 3. Microwave data were acquired by the Advanced Synthetic Aperture Radar (ASAR) instrument onboard ENVISAT. The multi-temporal capabilities and resolution (150m × 150m in WS mode) of the ASAR wide swath mode enabled the detection of dynamic features > 2 ha over this vast area. Scatterometer (QuikScat) data could be employed to distinguish hydro-periods. 4. Wetland types have been identified on the basis of seasonal changes in backscatter. In a first step scatterometer data were used to identify the transition period from frozen to unfrozen conditions over a range of 150 latitude. Inundation patterns and soil moisture changes could be identified for the different hydro-periods and used to classify wetlands. Results for peatlands have been compared with Russian forest inventory data which contain information on wetland distribution. 5. The database of permanently inundated areas is an intermediate product which enables the mapping of wetlands in two ways: (1) identification of seasonal inundation in relation to snowmelt and high permafrost tables and (2) input for density analysis of permanent small and shallow lakes in tundra areas which are important freshwater ecosystems as well as a methane source. Differences in intensity and duration of soil moisture conditions allow the identification of peatlands. [ABSTRACT FROM AUTHOR]

The present paper reports a case study about public deliberations in three Israeli kibbutzim regarding a disputed school issue: whether to maintain a traditional in-kibbutz high school despite a heavy financial burden or to close it and send kibbutz youths to a public regional school The results served as a demonstration of a ‘thinking group’ (i.e. of how the collective aims of a group are achieved by the coordinated rhetorical behaviour of individuals according to the formal rules of the collective deliberations). First, video-recordings of six general assembly meetings in which the issue was discussed was analyzed as to their argumentative content. Second, the extracted arguments were presented to a sample of 342 kibbutz members to capture the distribution of opinions in the population. It is proposed that most kibbutz members were willing to preserve their collective living and saw the closure of their in-kibbutz school as a threat to their traditional collective identity. We observed a distinct form of public rhetoric during the deliberations in the general meetings which provides a podium for the disputed opinions, preserves the kibbutz shared identity representation and avoids social friction. [ABSTRACT FROM AUTHOR]

Focuses on the potential of stem cells to overcome age-related deteriorations of the body in regenerative medicine. Molecules and processes that enable stem cells to initiate self-renewal and to divide, proliferate and then differentiate to rejuvenate damaged tissue; Medical potential of stem cell-based treatments; Impact of time and age on the basic units of life, which are the corresponding tissue-specific or adult stem cells.

Public opposition to genetically modified (GM) food and crops is widely interpreted as the result of the public's misperception of the risks. With scientific assessment pointing to no unique risks from GM crops and foods, a strategy of accurate risk communication from trusted sources has been advocated. This is based on the assumption that the benefits of GM crops and foods are self-evident. Informed by the interpretation of some qualitative interviews with lay people, we use data from the Eurobarometer survey on biotechnology to explore the hypothesis that it is not so much the perception of risks as the absence of benefits that is the basis of the widespread rejection of GM foods and crops by the European public. Some respondents perceive both risks and benefits, and may be trading off these attributes along the lines of a rational choice model. However, for others, one attribute—benefit—appears to dominate their judgments: the lexicographic heuristic. For these respondents, their perception of risk is of limited importance in the formation of attitudes toward GM food and crops. The implication is that the absence of perceived benefits from GM foods and crops calls into question the relevance of risk communication strategies for bringing about change in public opinion. [ABSTRACT FROM AUTHOR]

Little is known about the molecular machinery that directs secretory vesicles to the site of cell separation during cytokinesis. We show that in Saccharomyces cerevisiae, the class V myosin Myo2p and the Rab/Ypt Sec4p, that are required for vesicle polarization processes at all stages of the cell cycle, form a complex with each other and with a myosin light chain, Mlc1p, that is required for actomyosin ring assembly and cytokinesis. Mlc1p travels on secretory vesicles and forms a complex(es) with Myo2p and/or Sec4p. Its functional interaction with Myo2p is essential during cytokinesis to target secretory vesicles to fill the mother bud neck. The role of Mlc1p in actomyosin ring assembly instead is dispensable for this process. Therefore, in yeast, as recently shown in mammals, class V myosins associate with vesicles via the formation of a complex with Rab/Ypt proteins. Furthermore, myosin light chains, via their ability to be transported by secretory vesicles and to interact with class V myosin IQ motifs, can regulate vesicle polarization processes at a specific location and stage of the cell cycle. [ABSTRACT FROM AUTHOR]

Using data from policy analyses, media analyses and a European-wide survey about public perceptions of biotechnology conducted in 1996 and again in 1999, it is shown how a country's public develops an everyday understanding of a new technology (genetic modification) construed as potentially harmful by the media. To understand the reliance on images and related beliefs, we propose a theory of collective symbolic coping. It identifies four steps: first, the creation of awareness; second, production of divergent images; third, convergence upon a couple of dominant images in the public sphere; fourth, normalization. It is suggested that symbolic coping occurs in countries where a recent increase in policy activity and of media reporting has alerted the public; that this public show a high proportion of beliefs in menacing images; that these beliefs are relatively independent of pre-existing popular science knowledge; and that they are functionally equivalent to scientific knowledge in providing judgmental confidence and reducing self-ascribed ignorance. These propositions are shown to be true in Austria and Greece. Several implications of the theory are discussed, including social representation theory and public understanding of science. [ABSTRACT FROM AUTHOR]

One of the most relevant issues in future medicine is tissue regeneration. Transplantation medicine alone cannot solve the problem of incurable conditions of vital organs. One approach to this might be the replication of the spontaneous regeneration that is found in embryonic/neonatal tissue. In this study, a tissue model for basic investigation of regeneration mechanisms in vivo was established. We demonstrated by histology and immunohistochemical staining for types I and II collagen that neonatal rat cartilage unlike adult cartilage has the capacity for rapid scarfree regeneration after full‐thickness incision. The underlying mechanism was identified in the preserved proliferative capacity of neonatal chondrocytes. This in vivo model should prove useful in further studies of the role of cellular (e.g., GA cell cycle regulators) and extracellular (e.g., cytokines) factors in tissue regeneration and wound healing. (WOUND REP REG 2001;531–536) --> [ABSTRACT FROM AUTHOR]