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Key Clinical Message: Foreign bodies such as bone with a sharp end can penetrate the esophageal wall and lie extramurally. When a foreign body is not found on rigid oesophagoscopy, reassessing with imaging is important. The transcervical approach is a better alternative for such patients to remove the foreign bodies. [ABSTRACT FROM AUTHOR]

This case report documents the first confirmed outbreak of African swine fever (ASF) in a wild boar (Sus scrofa) in Nepal. The case was identified in a wild boar carcass found in Shuklaphanta National Park in February 2023. Post‐mortem findings, including splenomegaly, haemorrhagic lymph nodes and congested organs, were suggestive of ASF, which was confirmed by real‐time PCR. Epidemiological evidence indicates potential transmission from nearby ASF‐infected domestic pigs. This case underscores the critical need for rigorous biosecurity protocols, comprehensive surveillance and targeted wildlife management strategies to prevent further transmission between domestic pig and wild boar populations. [ABSTRACT FROM AUTHOR]

Electric distribution networks increasingly host distributed energy resources based on power electronic converter (PEC) toward active distribution networks (ADN). Despite advances in computational capabilities, electromagnetic transient models are limited in scalability because of their reliance on exact data about the distribution system and each of its components. Similarly, the use of the DER_A model, which is intended to examine the combined dynamic behavior of many DERs, is limited by the difficulty in parameterization. There is a need for improved dynamic models of DERs for use in large power system simulations for stability analysis. This paper proposes an aggregate model‐free, data‐driven approach for deriving a dynamic partitioned model (DPM) of ADNs. Detailed residential distribution feeders were first developed, including PEC‐based DERs and composite load models (CMLDs), from which the aggregated DPM was derived. The performance was evaluated through various case studies and validated against the detailed ADN model and state‐of‐the‐art DER_A model with CMLD. The data‐driven DPM achieved a fitpercent${\it fitpercent}$ of over 90%, accurately representing the aggregated dynamic behavior of ADNs. Furthermore, the DPM significantly accelerated the simulation process with a computational speedup of 68 times compared to the detailed ADN and a 3.5 times speedup compared to the DER_A CMLD model. [ABSTRACT FROM AUTHOR]

Key Clinical Message: Early initiation of anti‐retroviral treatment and prompt management of heart failure in a AIDS patient is crucial. If resources available endomyocardial biopsy can guide the future management. HIV‐associated cardiomyopathy (HIVAC) is a serious complication of HIV with poorly understood pathology. Systolic dysfunction was a common characteristic of HIVAC which is now replaced as diastolic dysfunction due to the availability of ART. There are various etiologies of HIVAC depending on the degree of viral replication, immune response, availability of ART, and also the disparity between high and low‐income countries. We present the case report of a 65‐year‐old male with a past medical history of untreated HIV who came to the hospital with hemodynamic instability and was diagnosed with HIVAC with pulmonary TB, where the cause of HIVAC was poor immune response and opportunistic infection. Therefore, early cardiac monitoring is essential in HIV patients to prevent its advancement. [ABSTRACT FROM AUTHOR]

Human fatalities and injury from wildlife attacks often result in a negative attitude toward conservation. This research was undertaken to investigate the patterns and conflict‐causing factors of human killing and injury by large mammals, especially by Asian elephant (Elephas maximus), common leopard (Panthera pardus), and Bengal tiger (Panthera tigris tigris) in the Bardia—Banke Complex of western Nepal. We collected human death and injury records caused by wildlife in the Bardia—Banke Complex between 2019 and 2023, based on relief applications submitted by the victim's family. Additionally, camera trap monitoring was conducted following incidents of human–tiger and human–leopard conflicts. A total of 76 incidents involving human casualties and injuries were considered for analysis. Incidents of livestock depredation, crop raiding, and property damage were excluded from the analysis. Most of the attacks on humans were caused by tigers (75%), followed by elephants (16%) and leopards (9%). Almost all incidents occurred in daytime (97%). The highest number of conflicts were recorded in 2021, with 20 incidents. Most of the cases (84%) occurred within 1 km of forest edge. Khata corridor and the western side of the Bardia National Park, i.e., Karnali River corridor, were identified as high‐conflict areas. The primary causes of the conflict manifested in cattle grazing (28%), grass cutting (28%), firewood collection (11%), fishing (8%), vegetable collection (5%), sand collection (4%), during rescuing friends (3%), grazing captive elephants (3%), highway rides (3%), sleeping in Chaupadi Goth (3%), walking nearby forest areas (3%), playing nearby forest areas (1%), while feeding pig (1%), and working in agricultural lands (1%). To promote human–wildlife coexistence, community‐based patrols (33%), habitat restoration (26%), electric fencing (26%), and insurance (7%) were identified as the preferred strategies. Therefore, we recommend that stakeholders and concerned bodies increase awareness among local community about the use of forest resources, wildlife behavior, and human–wildlife conflict mitigation strategies. [ABSTRACT FROM AUTHOR]

Cervical cancer remains a disease burden in Asia. The Asia and Oceania Federation of Obstetrics and Gynecology envisages a need to produce a set of recommendations on the implementation of human papilloma virus vaccination program for both lower‐middle‐income countries (LMICs) and high‐income countries (HICs), with an attempt to harmonize the practices yet allow flexibility to cater for different cultures, religions, needs and background of individual countries/cities. International guidelines and literature were sought, and recommendations were made in seven selected areas, including (i) the target groups for vaccination, (ii) the doses of vaccination including the use of single‐dose vaccination, (iii) the types of vaccines, (iv) suggestions for special populations including those with previous HPV infection, human immunodeficiency virus carriers, and lesbian, gay, bisexual, transgender, questioning/queer group, (v) inter‐changeability and the need of revaccination/booster, (vi) novel technologies and vaccines, and (vii) public education. [ABSTRACT FROM AUTHOR]

Key Clinical Message: Hypocalcemia is one of the rarest causes of reversible cardiomyopathy. Patients with refractory heart failure need to be explored for hypocalcemia and need prompt correction. Hypocalcemia is a rare cause of reversible dilated cardiomyopathy. Correction of calcium is crucial to recover left ventricular function and structure. We presented the case of a 55‐year‐old female who was admitted to the hospital with refractory heart failure due to hypocalcemia induced by primary hypoparathyroidism and complicated by vitamin D deficiency. The patient's cardiac symptoms improved dramatically upon correction of hypocalcemia, and vitamin D. Therefore, the key clinical message of this case report is, that hypocalcemia, although rare, should be considered as one of the differential diagnoses when heart failure is refractory and early diagnosis and treatment is necessary as it is the cause of reversible cardiomyopathy and could reduce morbidity and mortality. [ABSTRACT FROM AUTHOR]

Key Clinical Message: This case illustrates sarcoidosis as a potential complication of COVID‐19, highlighting the need for a comprehensive diagnostic approach, including histopathology and prolonged monitoring, to distinguish it from post‐COVID fibrosis. Further research is crucial to elucidate these associations and understand their underlying mechanisms. Severe Acute Respiratory Syndrome Coronavirus‐ 2 (SARS‐CoV‐2), a positive‐sense single‐stranded RNA virus, causes COVID‐19 and has been linked to autoimmune disorders. Sarcoidosis is a multi‐system disease that is frequently triggered by infections. It is characterized by non‐necrotizing granulomas in multiple organs. We present a case of sarcoidosis as rare sequelae of COVID‐19. A 26‐year‐old man presented with mild COVID‐19 symptoms, followed by prolonged fever and cough despite initial therapy, prompting a provisional diagnosis of post‐COVID fibrosis. A subsequent assessment at a tertiary hospital revealed dyspnea, weight loss, and abnormal chest imaging, all of which were consistent with pulmonary sarcoidosis with pulmonary tuberculosis as a differential diagnosis. A biopsy taken during bronchoscopy confirmed pulmonary sarcoidosis and treatment with inhalation steroids resulted in symptom relief, which was followed by remission with oral steroid therapy. Sarcoidosis is a systemic disease of unknown etiology, characterized by non‐necrotizing granulomas in multiple organs. It may be triggered by infections and involves an abnormal immune response. COVID‐19 can potentially initiate sarcoidosis, with both sharing common immune mechanisms. Diagnosis involves imaging and biopsy, and treatment typically includes glucocorticoids and regular monitoring. This case report emphasizes the potential link between COVID‐19 and autoimmune conditions like sarcoidosis, highlighting the need for a comprehensive diagnostic approach and long‐term observation to distinguish between sarcoidosis and post‐COVID fibrosis. [ABSTRACT FROM AUTHOR]

Key Clinical Message: Appropriate anesthetic technique for fracture fixation in multiple myeloma is crucial in minimizing perioperative complications. The use of pericapsular nerve group block followed by spinal anesthesia for the operative management of pathological fracture in multiple myeloma patient along with proper pre‐ and postanesthetic care ensures better outcome for the patient. Multiple myeloma is a malignant plasma cell disease that often presents with anemia, renal failure, hyperkalemia and osteolytic bone lesions. The advancements in drug therapy of multiple myeloma have prolonged the lifespan of the affected people, resulting in a rise in cases of surgical management of fractures in such patients. Anesthetic management, despite being of utmost importance in minimizing perioperative complications in such patients, has not been widely studied, especially in this part of the world. Hence, we report a case of 64 years diabetic, HbSAg positive male with hypothyroidism and a known case of multiple myeloma since the last 6 years, under medication for his comorbidities who suffered acetabular fracture. In this case report, the use of pericapsular nerve group block followed by spinal anesthesia for the operative management of the fracture has been discussed along with several pre‐ and postanesthetic considerations. With appropriate anesthetic techniques and proper pre‐ and postanesthetic care, better outcomes can be guaranteed. [ABSTRACT FROM AUTHOR]

Key Clinical Message: Ovarian hemangioma, though rare and asymptomatic, can mimic malignant ovarian tumors, thus it is necessary for comprehensive histopathological and immunohistochemical evaluation for accurate diagnosis and appropriate management. [ABSTRACT FROM AUTHOR]

This study examines the effect of friendly boards on capital allocation efficiency. We provide evidence that firms with friendly boards have a positive and statistically significant effect on capital allocation inefficiency. We find our results robust to different measures of friendly boards and capital allocation inefficiency, alternative model specifications, omitted variable bias, self‐selection bias and other endogeneity concerns. We also show that the positive association between friendly boards and capital allocation inefficiency is lower in firms with high external corporate governance quality but higher in firms with high financial constraints. The findings imply that poor board monitoring and high agency conflicts in firms with friendly boards lead to high capital allocation inefficiency. [ABSTRACT FROM AUTHOR]

Seafoods are susceptible to microbial contamination due to their high moisture, nutrient contents and neutral pH. Among various microorganisms, biofilm‐forming bacteria pose a severe threat to the seafood supply chain as well as consumer health. Bacterial biofilm formation in seafood is primarily caused by Aeromonas hydrophila, Vibrio cholerae, V. vulnificus, V. parahaemolyticus, Salmonella spp., and Listeria monocytogenes. Biofilm formation is an important protective mechanism of microorganisms causing spoilage of seafood and disease threats to consumers. The attachment of microbes on the surface of seafood followed by the growth and proliferation of bacterial cells leads to the production of exopolymer compounds and the formation of biofilm. This biofilm is difficult to destroy or inhibit through conventional prevention/destruction techniques. The occurrence of bacterial strains/biofilms with more resistance to different preventive strategies is a big challenge for the seafood processing industry. This review covers the mechanisms of biofilm formation by bacteria and various non‐thermal processing approaches to prevent microbial contamination and biofilm formation in seafood products. The aforementioned non‐thermal processing techniques for the destruction of biofilm and quality control of seafood products include cold plasma treatment, irradiation, pulsed electric field technology, hydrostatic pressure processing, photosensitisation, natural bioactive compounds and so on. All these techniques effectively inhibit the bacterial biofilm and microbial growth without altering sensorial properties. However, further research validation and applications at the industry level are still required. [ABSTRACT FROM AUTHOR]

Shelter‐building insects are important ecosystem engineers, playing critical roles in structuring arthropod communities. Nonetheless, the influence of leaf shelters and arthropods on plant–associated microbiota remains largely unexplored. Arthropods that visit or inhabit plants can contribute to the leaf microbial community, resulting in significant changes in plant–microbe interactions. By artificially constructing leaf shelters, we provide evidence that shelter‐building insects influence not only the arthropod community structure but also impact the phyllosphere microbiota. Leaf shelters exhibited higher abundance and richness of arthropods, changing the associated arthropod community composition. These shelters also altered the composition and community structure of phyllosphere microbiota, promoting greater richness and diversity of bacteria at the phyllosphere. In leaf shelters, microbial diversity positively correlated with the richness and diversity of herbivores. These findings demonstrate the critical role of leaf shelters in structuring both arthropod and microbial communities through altered microhabitats and species interactions. [ABSTRACT FROM AUTHOR]

Key Clinical Message: This case emphasizes the need for early recognition and accurate diagnosis of achalasia in young adults to avoid exacerbation of the condition and misdiagnosis as GERD. Patient outcomes and quality of life are greatly enhanced by suitable diagnostic techniques, appropriate therapy, interdisciplinary care, and comprehensive patient education along with frequent follow‐ups. Achalasia results from the degeneration of inhibitory ganglion cells within the esophageal myenteric plexus and the lower esophageal sphincter (LES), leading to a loss of inhibitory neurons and resulting in the absence of peristalsis with failure of LES relaxation. Its origins are multifactorial, potentially involving infections, autoimmune responses, and genetics, with equal incidence in males and females. The hallmark symptoms include progressive dysphagia for solids and liquids, along with regurgitation, heartburn, and non‐cardiac chest pain. A 22‐year‐old female patient initially diagnosed with gastroesophageal reflux disease (GERD) received proton pump inhibitors and antacid gel for persistent dysphagia and regurgitation. Subsequent tests including barium esophagogram and manometry indicated Type II Achalasia Cardia. The patient showed clinical improvement with relief of dysphagia, regurgitation, and heartburn symptoms after pneumatic balloon dilatation (PBD). She was advised to follow up after 6 months with upper gastrointestinal (UGI) endoscopy and manometry in the outpatient clinic for regular endoscopic surveillance as there is a risk of transformation to esophageal carcinoma. Diagnosing achalasia in young adults poses challenges due to its diverse presentation and resemblance to other esophageal disorders like GERD. Diagnosis relies on clinical symptoms and imaging studies such as barium esophagogram revealing a bird's beak appearance and esophageal manometry showing absent peristalsis. UGI endoscopy is needed to rule out malignancy. Treatment options include non‐surgical approaches like medication and Botox injections, as well as surgical methods such as pneumatic balloon dilation, laparoscopic Heller myotomy, and per‐oral endoscopic myotomy (POEM). The treatment options depend upon the patient's condition at presentation and their individual choices. This case report emphasizes that it is crucial to consider achalasia as a potential differential diagnosis in young adults with dysphagia, especially if conventional treatments for acid peptic disorder do not alleviate symptoms. Prompt diagnosis and appropriate management can lead to significant clinical improvement and better patient outcomes. [ABSTRACT FROM AUTHOR]

The properties of kale as a functional food are well established. We sought to determine how fermentation further enhances these properties. We tested different fermentation conditions: (i) spontaneous fermentation with naturally occurring bacteria, (ii) spontaneous fermentation with 2% salt, (iii) Lactococcus lactis, (iv) Lactobacillus acidophilus, (v) mixture of L. lactis and L. acidophilus, (vi) mixture of L. lactis, L. acidophilus, and Clostridium butyricum. We quantified selected bioactive components using high‐performance liquid chromatography (HPLC) and antinutritional factors using a gravimetric method and spectrophotometry. We then determined (i) the antioxidant capacity of the vegetable, (ii) anti‐inflammation capacity, and (iii) the surface microbiota composition by 16S sequencing. All fermentation methods imparted some benefits. However, fermentation with mixed culture of L. lactis and L. acidophilus was most effective in increasing polyphenols and sulforaphane accessibility, increasing antioxidant activity, and reducing antinutritional factors. Specifically, fermentation with L. lactis and L. acidophilus increased total polyphenols from 8.5 to 10.7 mgGAE/g (milligrams of gallium acid equivalent per gram) and sulforaphane from 960.8 to 1777 μg/g (microgram per gram) but decreased the antinutritional factors oxalate and tannin. Total oxalate was reduced by 49%, while tannin was reduced by 55%–65%. The antioxidant capacity was enhanced but not the anti‐inflammation potential. Both unfermented and fermented kale protected equally against lipopolysaccharide (LPS)‐induced inflammation in RAW 264.7 macrophages and prevented increases in inducible nitric oxide synthase (iNOS), tumor necrosis factor‐alpha (TNF‐α), interleukin‐1 beta (IL‐1β), and interleukin‐6 messenger RNA (IL‐6 mRNA) expression by 84.3%, 62%, 68%, and 85.5%, respectively. Unfermented and naturally fermented kale had high proportions of sulfur reducing Desulfubrio and Proteobacteria usually associated with inflammation. Fermenting with L. lactis and/or L. acidophilus changed the bacterial proportions, reducing the Proteobacteria while increasing the genera Lactobacilli and Lactococcus. In summary, fermentation enhances the well‐known beneficial impacts of kale. Fermentation with mixed cultures of L. lactis and L. acidophilus imparts higher benefits compared to the single cultures or fermentation with native bacteria present in the vegetable. [ABSTRACT FROM AUTHOR]

Background: Outpatient parenteral antimicrobial treatment (OPAT) is a safe and effective therapy used in several settings across Australia. As OPAT services expand their inclusion criteria to include complex patient populations, there is an increased need for selecting appropriate patients to receive either healthcare‐administered OPAT (H‐OPAT) or self‐administered OPAT (S‐OPAT). Aims: To describe patient demographics, diagnosis, microbiology and outcomes of patients treated by H‐OPAT and S‐OPAT within the Sunshine Coast Hospital and Health Service, Australia. Methods: Data on demographics, diagnoses, treatment and outcomes on all patients treated by H‐OPAT and S‐OPAT from March 2017 to December 2019 were collected retrospectively. Results: One hundred and sixty‐five patients (62.26%) were enrolled in H‐OPAT and 100 patients (37.74%) in S‐OPAT. S‐OPAT patients were significantly younger. H‐OPAT patients were more comorbid. Bone and joint infections were the most treated infections and were more likely to be treated by S‐OPAT. There was no difference in treatment duration, cure and complication rates between S‐OPAT and H‐OPAT. Longer duration of therapy was associated with more complications. Treatment failure was associated with infections due to multiple organisms, number of comorbidities and treatment of surgical site, skin and soft tissue infections. Conclusions: There were significant differences in demographics between H‐OPAT and S‐OPAT without any difference in outcomes. Overall failure and complication rates were low. Higher rates of treatment failure were predicted by the diagnosis, number of comorbidities and number of organisms treated. [ABSTRACT FROM AUTHOR]

The 'landscape of fear' concept offers valuable insights into wildlife behaviour, yet its practical integration into habitat management for conservation remains underexplored. In this study, conducted in the subtropical monsoon grasslands of Bardia National Park, Nepal, we aimed to bridge this gap through a multi‐year, landscape‐scale experimental investigation in Bardia National Park, Nepal. The park has the highest density of tigers (with an estimated density of ~7 individuals per 100 km2) in Nepal, allowing us to understand the effect of habitat management on predation risk and resource availability especially for three cervid species: chital (Axis axis), swamp deer (Rucervus duvaucelii) and hog deer (Axis porcinus). We used plots with varying mowing frequency (0–4 times per year), size (ranging from small: 49 m2 to large: 3600 m2) and artificial fertilisation type (none, phosphorus, nitrogen) to assess the trade‐offs between probable predation risk and resources for these cervid species, which constitute primary prey for tigers in Nepal. Our results showed distinct responses of these deer to perceived predation risk within grassland habitats. Notably, these deer exhibited heightened use of larger plots, indicative of a perceived sense of safety, as evidenced by the higher occurrence of pellet groups in the larger plots (mean = 0.1 pellet groups m−2 in 3600 m2 plots vs. 0.07 in 400 m2 and 0.05 in 49 m2 plots). Furthermore, the level of use by the deer was significantly higher in larger plots that received mowing and fertilisation treatments compared to smaller plots subjected to similar treatments. Of particular interest is the observation that chital and swamp deer exhibited greater utilisation of the centre (core) areas within the larger plots (mean = 0.21 pellet groups m−2 at the centre vs. 0.13 at the edge) despite the edge (periphery) also provided attractive resources to these deer. In contrast, hog deer did not display any discernible reaction to the experimental treatments, suggesting potential species‐specific variations in response to perceived predation risk arising from management interventions. Our findings emphasise the importance of a sense of security as a primary determinant of habitat selection for medium‐sized deer within managed grassland environments. These insights carry practical implications for park managers, providing a nuanced understanding of integrating the 'landscape of fear' into habitat management strategies. This study emphasises that the 'landscape of fear' concept can and should be integrated into habitat management to maintain delicate predator–prey dynamics within ecosystems. [ABSTRACT FROM AUTHOR]

Agro‐morphological traits play a significant role in the adaptation of wheat to diverse agroecosystems. Genetic understanding of these traits is crucial to develop cultivars adapted to specific environments and maximize their productivity. This is a comprehensive genome‐wide association study (GWAS) of 230 diverse lines of soft red winter wheat for identifying quantitative trait loci (QTLs) related to eight key agro‐morphological traits. The diversity panel was evaluated in two locations for three consecutive years (2020–2023). A total of 150 significant marker–trait associations were detected, including 65 for three flag leaf traits, 35 for peduncle length, 33 for plant height, 16 for heading date, and one for plant vigor using 27,466 single nucleotide polymorphism (SNP) markers. Eleven high‐confidence major‐effect QTLs explaining greater than 10% phenotypic variance were detected, of which seven were stable, and one showed an association with plant height and peduncle length. QTLs possibly allelic for important dwarfing (Rht23) and vernalization (Vrn‐B1) genes were identified. Six QTLs, QFlw.uga‐1A, QPdl.uga‐1A, QFlw.uga‐2B.2, QPdl.uga‐5A, QPdl.uga‐7A, and QPht.uga‐7B, are presumed to be novel, and nearby candidate gene(s) were identified for all except QPdl.uga‐1A. The pyramiding of favorable alleles from major‐effect QTLs was found to have significant improvement in peduncle length (shortened by 5 cm), flag leaf width (increased by 0.18 cm), and plant height (shortened by 11 cm). This study has improved our genetic understanding of important agro‐morphological traits. These results, upon further validation, can be used in breeding for desirable plant architecture to improve wheat yield potential. Core Ideas: Genome‐wide association study (GWAS) was conducted using 230 wheat lines evaluated for eight agro‐morphological traits.One hundred fifty significant marker–trait associations were found using 27,466 single nucleotide polymorphism (SNP) markers.Eleven major‐effect quantitative trait loci (QTLs) including seven stable QTLs were identified.Six major‐effect QTLs were presumed to be novel, and candidate genes were identified for five of them.Pyramiding favorable alleles from these QTLs led to significant improvement in traits. [ABSTRACT FROM AUTHOR]

Silicon and Germanium oxide (SiOx and GeOx) nanostructures are promising materials for energy storage applications due to their potentially high energy density, large lithiation capacity (~10X carbon), low toxicity, low cost, and high thermal stability. This work reports a unique approach to achieving controlled synthesis of SiOx and GeOx nanostructures via photonic curing. Unlike conventional methods like rapid thermal annealing, quenching during pulsed photonic curing occurs rapidly (sub‐millisecond), allowing the trapping of metastable states to form unique phases and nanostructures. We explored the possible underlying mechanism of photonic curing by incorporating laws of photophysics, photochemistry, and simulated temperature profile of thin film. The results show that photonic curing of spray coated 0.1 M molarity Si and Ge Acetyl Acetate precursor solution, at total fluence 80 J cm−2 can yield GeOx and SiOx nanostructures. The as‐synthesized nanostructures are ester functionalized due to photoinitiated chemical reactions in thin film during photonic curing. Results also showed that nanoparticle size changes from ~48 nm to ~11 nm if overall fluence is increased by increasing the number of pulses. These results are an important contribution towards large‐scale synthesis of the Ge and Si oxide nanostructured materials which is necessary for next‐generation energy storage devices. [ABSTRACT FROM AUTHOR]

Although in silico folding based on coevolving residue constraints in the deep‐learning era has transformed protein structure prediction, the contributions of coevolving residues to protein folding, stability, and other functions in physical contexts remain to be clarified and experimentally validated. Herein, the PHD finger module, a well‐known histone reader with distinct subtypes containing subtype‐specific coevolving residues, was used as a model to experimentally assess the contributions of coevolving residues and to clarify their specific roles. The results of the assessment, including proteolysis and thermal unfolding of wildtype and mutant proteins, suggested that coevolving residues have varying contributions, despite their large in silico constraints. Residue positions with large constraints were found to contribute to stability in one subtype but not others. Computational sequence design and generative model‐based energy estimates of individual structures were also implemented to complement the experimental assessment. Sequence design and energy estimates distinguish coevolving residues that contribute to folding from those that do not. The results of proteolytic analysis of mutations at positions contributing to folding were consistent with those suggested by sequence design and energy estimation. Thus, we report a comprehensive assessment of the contributions of coevolving residues, as well as a strategy based on a combination of approaches that should enable detailed understanding of the residue contributions in other large protein families. [ABSTRACT FROM AUTHOR]

Problem: Early‐onset preeclampsia (EOPE) is a severe gestational hypertensive disorder with significant feto‐maternal morbidity and mortality due to uteroplacental insufficiency. Circulating extracellular vesicles of placental origin (EV‐P) are known to be involved in the pathophysiology of EOPE and might serve as an ideal reservoir for its specific biomarkers. Therefore, we aimed to characterize and perform comparative proteomics of circulating EV‐P from healthy pregnant and EOPE women before delivery. Method of Study: The EV‐P from both groups were isolated using immunoaffinity and were characterized using transmission electron microscopy, dynamic light scattering, nanoparticle tracking analysis, and immunoblotting. Following IgG albumin depletion, the pooled proteins that were isolated from EV‐P of both groups were subjected to quantitative TMT proteomics. Results: Circulating term EV‐P isolated from both groups revealed ∼150 nm spherical vesicles containing CD9 and CD63 along with placental PLAP and HLA‐G proteins. Additionally, the concentration of EOPE‐derived EV‐P was significantly increased. A total of 208 proteins were identified, with 26 among them being differentially abundant in EV‐P of EOPE women. This study linked the pathophysiology of EOPE to 19 known and seven novel proteins associated with innate immune responses such as complement and TLR signaling along with hemostasis and oxygen homeostasis. Conclusion: The theory suggesting circulating EVs of placental origin could mimic molecular information from the parent organ—"the placenta"—is strengthened by this study. The findings pave the way for possible discovery of novel prognostic and predictive biomarkers as well as provide insight into the mechanisms driving the pathogenesis of EOPE. [ABSTRACT FROM AUTHOR]

Despite the implementation of progressive national policies and the adherence to numerous international conventions aimed at eradicating gender‐based violence and violence against women, incidents of violence against women in post‐war Sri Lanka are on the rise. This research delves into the intersection of post‐war populist politics supported by religious extremists and the resulting violence perpetrated against women, as well as its repercussions on women's well‐being. We are interested in locating the specific mechanisms through which religious extremism, authoritarian populism, and violence against women are connected. Our findings indicate that post‐war populist politics, backed by Buddhist religious extremists and the post‐war Sinhalese‐Buddhist hegemonic state's re‐building project, have generated dialectical relationships with Sri Lanka's minorities—Tamils and Muslims. These relationships are partially enacted through different forms of gender violence. This has consequences for all women. As successive confrontations deepen, the deterioration of these relationships exacerbates the likelihood of violence and reinforces a culture of impunity. [ABSTRACT FROM AUTHOR]

Religious sentiments have become a potent tool for political elites, altering national identities and citizenship criteria. This manipulation, driven by majoritarian ideologies, has marginalized religious minorities, leading to discrimination, violence, and legal persecution against them. Hindu, Islamic, and Buddhist nationalisms seem to collectively erode secular democracy in South Asia. Moreover, right‐wing populist leaders, notably Modi in India and the Rajapaksas in Sri Lanka, have merged religious nationalism with authoritarian political orientations posing a challenge to democratic foundations and impacting significantly on the dynamics of state and societal relations. This is the context in which this World Affairs 2024 special issue delves into the relationship(s) between religion, politics, and authoritarianism in the South Asian region. It examines the evolving "religion‐politics" nexus or "complex" to explicate the relationship between religion and authoritarianism, particularly with respect to authoritarian governments mobilizing religion as a readily available resource for popular political mobilization, legitimization, and power consolidation. India's BJP employs Hindutva ideology to marginalize minorities, Bangladesh's regime uses Islam for political legitimacy, and Sri Lanka's Sinhala‐Buddhist nationalism fosters exclusionary policies. By fostering social and cultural polarization, this fusion of authoritarianism with religious nationalism poses a serious challenge to the secular state. The articles in this special issue explore these themes comprehensively, delving into the ideological foundations of political decisions and their consequences for marginalized communities. By shedding light on this multifaceted interplay, the collection offers valuable insights into South Asia's sociopolitical landscape, serving as a vital resource for scholars, policy makers, and global observers seeking to understand the region's complex dynamics. [ABSTRACT FROM AUTHOR]

Aim and Questions: Sea‐level rise has been responsible for extensive vegetation changes in coastal areas worldwide. The intent of our study was to analyze vegetation dynamics of a South Florida coastal watershed within an explicit spatiotemporal framework that might aid in projecting the landscape's future response to restoration efforts. We also asked whether recent transgression by mangroves and other halophytes has resulted in reduced plant diversity at local or subregional scales. Location: Florida''s Southeast Saline Everglades, USA. Methods: We selected 26 locations, representing a transition zone between sawgrass marsh and mangrove swamp, that was last sampled floristically in 1995. Within this transition zone, leading‐ and trailing‐edge subzones were defined based on plant composition in 1995. Fifty‐two site × time combinations were classified and then ordinated to examine vegetation–environment relationships using 2016 environmental data. We calculated alpha‐diversity using Hill numbers or Shannon–Weiner index species equivalents and compared these across the two surveys. We used a multiplicative diversity partition to determine beta‐diversity from landscape‐scale (gamma) diversity in the entire dataset or in each subzone. Results: Mangrove and mangrove associates became more important in both subzones: through colonization and establishment in the leading edge, and through population growth combined with the decline of freshwater species in the trailing edge. Alpha‐diversity increased significantly in the leading edge and decreased nominally in the trailing edge, while beta‐diversity declined slightly in both subzones as well as across the study area. Conclusions: Recent halophyte encroachment in the Southeast Saline Everglades continues a trend evident for almost a century. While salinity is an important environmental driver, species' responses suggest that restoration efforts based on supplementing freshwater delivery will not reverse a trend that depends on multiple interacting factors. Sea‐level‐rise‐driven taxonomic homogenization in coastal wetland communities develops slowly, lagging niche‐based changes in community structure and composition. [ABSTRACT FROM AUTHOR]

Background: Previous meta‐analyses have shown mixed results regarding the association between eating disorders (EDs) and type 1 diabetes mellitus (T1DM). Our paper aimed to analyse different EDs and disordered eating behaviours that may be practiced by patients with T1DM. Methods: A literature search of PubMed, Scopus and Web of Science was conducted on 17 January 2023, using the key terms "T1DM," "Eating Disorders" and "Bulimia." Only observational controlled studies were included. The Revman software (version 5.4) was used for the analysis. Results: T1DM was associated with increased risk of ED compared with nondiabetic individuals (RR = 2.47, 95% CI = 1.84–3.32, p‐value < 0.00001), especially bulimia nervosa (RR = 2.80, 95% CI = 1.18–6.65, p‐value = 0.02) and binge eating (RR = 1.53, 95% CI = 1.18–1.98, p‐value = 0.001). Our analysis has shown that increased risk of ED among T1DM persisted regardless of the questionnaire used to diagnose ED; DM‐validated questionnaires (RR = 2.80, 95% CI = 1.91–4.12, p‐value < 0.00001) and generic questionnaires (RR = 2.03, 95% CI = 1.27–3.23, p‐value = 0.003). Prevalence of insulin omission/misuse was 10.3%; diabetic females demonstrated a significantly higher risk of insulin omission and insulin misuse than diabetic males. Conclusion: Our study establishes a significant and clear connection between EDs and T1DM, particularly bulimia and binge eating, with T1DM. Moreover, female diabetics are at higher risk of insulin misuse/omission. Early proactive screening is essential and tailored; comprehensive interventions combining diabetes and ED components are recommended for this population, with referral to a specialised psychiatrist. [ABSTRACT FROM AUTHOR]

Background: Virtual ward (VW) models of care established during the coronavirus disease 2019 (COVID‐19) pandemic provided safe and equitable provision of ambulatory care for low‐risk patients; however, little is known about patients who require escalation of care to hospitals from VWs. Aim: To assess our VW model of care and describe the characteristics of patients admitted to the hospital from the VW. Methods: Observational study of all patients admitted to a tertiary hospital COVID‐19 VW between 1 December 2021 and 30 June 2022. Utilisation and epidemiological characteristics were assessed for all patients while additional demographics, assessments, treatments and outcomes were assessed for patients admitted to the hospital from the VW. Results: Of 9494 patient admissions, 269 (2.83%) patients identified as Aboriginal and Torres Strait Islander and 1774 (18.69%) were unvaccinated. The median length of stay was 5.10 days and the mean Index of Relative Socio‐economic Advantage and Disadvantage decile was 5.73. One hundred sixty (1.69%) patients were admitted to the hospital from the VW, of which 25 were adults admitted to medical wards. Of this cohort, prominent comorbidities were obesity, hypertension, asthma and frailty, while the main symptoms on admission to the VW were cough, fatigue, nausea and sore throat. High Pandemic Respiratory Infection Emergency System Triage (PRIEST), Veterans Health Administration COVID‐19 (VACO), COVID Home Safely Now (CHOSEN) and 4C mortality scores existed for those readmitted. Conclusions: This VW model of care was both safe and effective when applied to a broad socioeconomic population during the COVID‐19 pandemic. While readmission to the hospital was low, this study identified key characteristics of such presentations, which may assist future triaging, escalation and resource allocation within VWs during the COVID‐19 pandemic and beyond. [ABSTRACT FROM AUTHOR]

Key Clinical Message: Although it is very uncommon, SLE may initially present with recurrent episodes of EM‐like rash. Despite the various possibilities underlying their association, prompt identification, and treatment of SLE in patients presenting with EM is important to prevent death or serious organ damage. Rowell's syndrome (RS) is an uncommon presentation of systemic lupus erythematosus (SLE) with erythema multiforme (EM)‐like lesions associated with specific serological changes, including positive rheumatoid factor (RF), speckled antinuclear antibody (ANA), positive rheumatoid factor, or anti‐La antibodies in the serum. Our case, a 41‐year‐old male, presented with features of EM. Upon investigation, we identified underlying systemic lupus erythematosus, marking a rare instance of SLE presenting for the first time as EM. Classical or true EM is precipitated by trigger factors such as infective agents like the herpes simplex virus, Mycoplasma pneumoniae, drugs like anticonvulsants, antibiotics, and non‐steroid anti‐inflammatory drugs, any underlying malignancy, or connective tissue disorders, and is not associated with any specific serological abnormalities. EM cases associated with LE lesions where an EM trigger factor is missing are considered an RS diagnostic criterion. In this case report, the importance of considering SLE in patients presenting initially with recurrent episodes of EM‐like rash is emphasized. RS should be considered, especially when there is no evidence of triggering factors. Early diagnosis and prompt treatment of SLE are crucial to preventing death and irreversible organ damage. [ABSTRACT FROM AUTHOR]

This paper examines the payments made to minority producers, focused on African American producers, from the COVID‐19 program, Coronavirus Food Assistance Program (CFAP), of the United States Department of Agriculture (USDA) and compares it with one of the other more recent ad hoc program payments, the Market Facilitation Program (MFP). There were two rounds of the CFAP, and combinedly (as of March 2022), the program made direct payments of $31.0 billion ($11.8 billion from CFAP 1 and $19.2 billion from CFAP 2) starting in 2020. The MFP made a total payment of $23.5 billion (in two rounds, MFP 2018 and MFP 2019) to producers affected by the retaliatory tariffs placed on US producers by trade partners across multiple years. CFAP made almost $600 million in direct payments to minority producers, including Black or African American producers. Black or African American only producers received more than $52 million in CFAP payments. CFAP payments were proportional to the value of agricultural commodity sold for most minority producers. The 2017 Census of Agriculture showed that the majority of minority producers, including African American producers but excluding Asian producers, raised livestock. CFAP made the highest payments to livestock minority producers. The CFAP payment distribution pattern shows that payments reached minority producers who often did not receive Government payments. CFAP made more payments and as a share of total program outlays to minority producers compared to MFP. However, for Black or African American only producers, even though the magnitude increased (because CFAP disbursed more funds compared to MFP), the share of payment received did not increase. [ABSTRACT FROM AUTHOR]

Using data from the 2009–2020 Agricultural Resource Management Surveys, we compare the financial position and performance of African American farms to that of other U.S. farms. The results suggest that, relative to others, the average African American farm has lower total value of production, net cash farm income, government payments, assets, and debts. We obtained mixed results regarding financial performance, with African American farms having lower profitability, liquidity, and efficiency than others but higher solvency. To our knowledge, this study is the first to provide a contemporary overview of the status of African American farms using detailed, farm‐level financial data. [ABSTRACT FROM AUTHOR]

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Inflammation is a common condition of prostate tissue, whose impact on carcinogenesis is highly debated. Microbial colonization is a well-documented cause of a small percentage of prostatitis cases, but it remains unclear what underlies the majority of sterile inflammation reported. Here, androgen- independent fluctuations of PSA expression in prostate cells have lead us to identify a prominent function of the Transient Receptor Potential Cation Channel Subfamily M Member 8 (TRPM8) gene in sterile inflammation. Prostate cells secret TRPM8 RNA into extracellular vesicles (EVs), which primes TLR3/NF-kB-mediated inflammatory signaling after EV endocytosis by epithelial cancer cells. Furthermore, prostate cancer xenografts expressing a translation-defective form of TRPM8 RNA contain less collagen type I in the extracellular matrix, significantly more infiltrating NK cells, and larger necrotic areas as compared to control xenografts. These findings imply sustained, androgen-independent expression of TRPM8 constitutes as a promoter of anticancer innate immunity, which may constitute a clinically relevant condition affecting prostate cancer prognosis. Synopsis: The molecular origins of chronic tissue inflammation and its impact on cancer growth remain elusive. This study identifies TRPM8 transcripts as androgen-independent driver of sterile inflammation in the prostate gland, promoting anticancer innate immunity in the tumor microenvironment. TRPM8 RNA is secreted via extracellular vesicles (EVs) by normal and prostate cancer cells in the absence of cell damage. Upon EV endocytosis, TRPM8 mRNA binds TLR3 in endosomes, thereby promoting NF-kB/IRF3 activation and release of pro-inflammatory signals. NF-kB induces the androgen-independent expression of PSA encoded by the KLK3 gene. TRPM8/TLR3 signaling induces inflammation in prostate cancer xenografts, promoting infiltration and anticancer activity of NK cells. TLR3-activation by extracellular vesicle-delivered TRPM8 mRNA triggers aseptic inflammation in the prostate epithelium, promoting tumor suppression by NK cells. [ABSTRACT FROM AUTHOR]

In areas with widespread prevalence of myth of goat milk as a platelet booster, the goat milk can be connecting link in dengue and brucella coinfection. [ABSTRACT FROM AUTHOR]

Key Clinical Message: Patients mimicking appendicitis symptoms in a rural setting or those post‐appendectomy, indicating cecal volvulus, should always be considered. Swift action can prevent catastrophic consequences. We present a case of a 14‐year‐old female who initially underwent open appendectomy for acute appendicitis and subsequently experienced symptoms of abdominal distention, vomiting, and fever. Her condition deteriorated following the appendectomy, despite a prior appendectomy for similar symptoms at a different facility. A computed tomography (CT) scan identified cecal volvulus as the underlying issue. This led to the performance of a laparotomy, cecopexy, and decompressive ileostomy. After six weeks, ileostomy closure was successfully carried out, and the patient currently enjoys good health. This case highlights the significance of considering uncommon factors as potential contributors to postoperative complications in young patients. [ABSTRACT FROM AUTHOR]

Globally, biodiversity is declining due to habitat loss and degradation, over‐exploitation, climate change, invasive species, pollution, and infrastructure development. These threats affect the populations of large waterbird species, such as Sarus crane (Grus antigone), which inhabits agricultural–wetland ecosystems. Despite the burgeoning built‐up areas and diminishing agricultural and wetland spaces, scant research investigates the impact of these changing land uses on the globally vulnerable Sarus crane in Nepal. During the pre‐breeding season from April to June 2023, our comprehensive study meticulously scrutinized Sarus crane population status and factors associated with the occurrences and conservation challenges across 10 specific districts of Nepal. Our study documented a total of 690 individuals of Sarus cranes in five districts. The Lumbini Province has 685 individuals, occupying 11 roosting sites. Conversely, the remaining five districts have no Sarus cranes presence during this period. Wetland, farmland and built‐up areas exhibited a significantly positive influence on Sarus crane occurrences in the Lumbini Province. Additionally, we recorded 47 fatalities of Sarus cranes over the past 13 years in the Lumbini Province due to electrocution and collisions. Our study provides a baseline dataset crucial for developing conservation policies, particularly during the dry season when Sarus crane populations tend to congregate in larger flocks. The adaptation of the Sarus crane to urbanized landscapes exposes them to several anthropogenic threats in the coming days. Therefore, protecting wetlands and farmland areas and adopting transboundary conservation approaches are imperative for the long‐term conservation of the Sarus crane and its habitat. [ABSTRACT FROM AUTHOR]

Objectives: This study was conducted to assess oral hygiene practices, oral health status and barriers to utilization of oral health care services among pregnant mothers attending two family health care clinics in Sunsari, Nepal. Methods: A cross‐sectional study was conducted among 139 women using a purposive sampling technique. The data collection was done using a pretested standard semi‐structured questionnaire. Face‐to‐face interviews of the participants were done by a single investigator in the local language (Nepali). The examination was done using a mouth mirror and CPI probe for periodontal status, loss of attachment and dentition status, and treatment needs. Results: The majority of pregnant mothers brushed their teeth once a day or less than once a day (n = 106, 76.3%) and self‐reported perceived oral health status was poor/fair (n = 93, 66.9%). The prevalence of dental caries was found to be 69.8%. Bleeding on probing was present in all participants. DMFT, presence of bleeding on probing and increased periodontal pocket was significantly high among women who had self‐reported their oral health problems in comparison to those who had not reported any problem. The most common barriers reported by the participants were a lack of knowledge of dental checkups and a lack of perceived need for dental care during pregnancy. Conclusions: There was a high prevalence of dental caries and periodontal disease. Lack of knowledge and perceived need for dental care were the major barriers found in this study. Hence, this directs towards the utmost need for improvement in awareness level as well as oral hygiene practices. [ABSTRACT FROM AUTHOR]

Rice landraces conserved by tribal farmers are important for their nutritional richness. Landraces are rich in essential amino acids, vitamins, anthocyanins, and flavonoids useful to cure noncommunicable diseases and metabolic disorders. A study was carried out with 10 rice landraces from the tribal‐dominated belt of Southern Odisha to investigate grain nutrition, proximate composition, and vitamin and mineral contents. The protein content of the landraces was higher (>6 g/100 g) and the fat content was lower (<0.6 g/100 g) than popular Indian rice varieties. The mean nutrient content of 10 rice landraces was as follows: protein 6.3 ± 0.313 g/100 g, total dietary fiber 1.6 ± 0.094 g/100 g, fat 0.536 ± 0.008 g/100 g, ash 10.514 ± 6.753%, and total sugar 77.18 ± 2.118 g/100 g. The high genotypic coefficient of variation (GCV) was observed for alkali spreading value (31.11%), capacity of grain hydration (52.705), index of hydration (171.439), moisture (46.343%), and vitamin B2 (23.994%) in rice landraces. Few landraces had superior iron content: Kalamalli (1.49 mg/100 g), Kandulakathi (1.42 mg/100 g), and Dudhamani (1.39 mg/100 g) compared to popular Indian rice varieties. Tikichudi had highest moisture (19%) and fat (0.53 g/100 g) content, which signifies the taste quality of rice. Kanakchudi exhibited the highest fiber content (1.8 g/100 g) and ash content (22.80%). Kalamalli contained higher zinc (0.49 mg/100 g), iron (1.49 mg/100 g), potassium (108.33 mg/100 g), magnesium (78.33 mg/100 g), and phosphorus (125.00 mg/100 g), whereas Muktabali was found to have higher Ca (3.88 mg/100 g) and Baunsidubraj exhibited higher niacin (4.9 mg/100 g). The indigenous landraces Kalamalli, Kandulakathi, and Dudhamani had considerably high iron content, whereas Kalamalli, Baunsidubraj, and Muktabali possessed less phytic acid in comparison with existing varieties and other landraces reported from various states of our country. Landraces Kalamalli, Kanakchudi, Tikichudi, and Muktabali from southern Odisha, India, represented nutritionally better genetic pool for future rice improvement. [ABSTRACT FROM AUTHOR]

Coastal dry tropical forests (CDTFs) are important yet vulnerable ecosystems. In this paper, we highlight the special conservation issues facing CDTFs by focusing on one variant of the type, those that occupy limestone substrate in the northeastern Caribbean. Our analysis draws largely from the coastal terrestrial broadleaf forests of the northern Bahamas, the Florida Keys, and southwestern Puerto Rico. Based on the surveys of storm surge recorded during major hurricanes during the last 50 years, we define CDTFs as coastal terrestrial broadleaf forests on ground surfaces elevated up to 5 m above sea level and occurring within 5 km of the coast. These forests are not only threatened by land‐use change from urbanization but also climate‐driven sea level rise (SLR) and hurricanes, which have degraded them and reduced their extent. CDTFs are distinguished from other dry tropical forests by the occasional influence of marine water incursion during periodic storms, requiring species common to these forests to have some level of salt tolerance despite experiencing well‐drained, fresh water conditions during most of their life span. With precipitation being the sole freshwater source for most CDTFs, SLR and the resulting salinization in the rooting zone subjects these forests to increasingly stressful conditions. Hence, even a modest rise in sea level can push numerous imperiled and endangered species and coastal terrestrial broadleaf communities to the edge of their tolerance, causing a decline in extent or their complete disappearance. Outside of protected areas, rapid urbanization has fragmented these forests and reduced their extent, which in turn have modified the interaction between rising seas and forest function. This work emphasizes the need for refined risk assessments to be completed and for conservation measures to be enforced so that resources can be directed appropriately to prevent further loss of CDTFs. Abstract in Spanish is available with online material. [ABSTRACT FROM AUTHOR]

Pre‐release host range assessments of weed biocontrol agent (BCA) candidates typically rely on no‐choice and choice feeding, oviposition, and development tests. However, these tests may exclude potentially environmentally safe BCA candidates from consideration if they develop on nontarget plant species in no‐choice tests that they would not colonize in the field because of behavioral barriers during host selection. Here, we examined the behavioral responses of Ceutorhynchus rusticus Gyllenhal (Coleoptera: Curculionidae) to olfactory and visual plant cues of nine native North American and two Eurasian confamilial plant species of invasive Eurasian mustard dyer's woad, Isatis tinctoria L. (Brassicaceae). In behavioral bioassays with olfactory, visual, and combined plant cues of one plant species vs. purified air and/or empty arms (control treatments), weevils responded with attraction to all offered I. tinctoria plant cues but showed no preference for most nontarget plant species relative to control treatments. The weevil also responded faster in these bioassays to I. tinctoria cues when compared to most nontarget species. In bioassays with olfactory, visual, or combined cues of nontarget species vs. those of I. tinctoria, C. rusticus preferred I. tinctoria over most nontarget plant species (olfactory cues: eight of 11, visual cues: two of 11, and combined cues: 11 out of 11 nontarget plant species). The results also suggest that C. rusticus mainly uses olfactory or combined olfactory and visual plant cues rather than visual cues alone in the early stages of host finding. Additionally, the findings suggest that during host finding, C. rusticus prefers cues of its Eurasian field host over those of nontarget species, reducing risks of post‐release nontarget attack in the field. Assessing behavioral responses to multimodal cues can advance our understanding of BCA candidates' host discrimination and facilitate the accurate interpretation of conventional no‐choice and choice feeding and development data during pre‐release environmental safety assessments. [ABSTRACT FROM AUTHOR]

Key Clinical Message: We report the case of chyluria secondary to disseminated tuberculosis in a 13‐year‐old female child who presented with passage of white colored urine since 5 months, progressive weight loss for 3 months, abdominal distension for 2 months, generalized swelling of body for 15 days, and pain in abdomen for 10 days. Child had good recovery following treatment with antitubercular drugs. Though chyluria is uncommon in children, tuberculosis could be considered as a differential, after ruling out filariasis. [ABSTRACT FROM AUTHOR]

Key Clinical Message: Dermatomyositis is an uncommon autoimmune disease with only few cases reported from Nepal. Presence of anti TIF‐1 gamma antibodies in DM are the strongest predictor of malignancy. Timely screening of malignancies for early detection and management remains the mainstay of this report. [ABSTRACT FROM AUTHOR]

Nepal initiated numerous hydropower and irrigation‐related infrastructure projects to enhance and promote green energy, water security, and agricultural productivity. However, these projects may pose risks to natural habitats and the well‐being of aquatic fauna, leading to significant effects on delicate ecosystems. To understand these potential impacts, it is crucial to gather reliable baseline data on the population status and habitat characteristics of species. This study specifically focuses on Gharials (Gavialis gangeticus), a critically endangered species. We recorded data on pre‐determined habitat variables at stations spaced 500 m apart along the two major river streams of Bardia National Park, as well as at locations where Gharials were sighted between February and March 2023. We used binary logistic regression with a logit link function to investigate the habitat characteristics related to the occurrence of Gharials. The presence/absence of Gharials at sampling points served as the dependent variable, while 10 other predetermined variables (ecological variables and disturbance variables) served as independent variables. Our study recorded 23 Gharials, comprising 14 adults, six sub‐adults, and three juveniles, with a sex ratio of 55.56 males per 100 females. Most individuals (83%) were found basking. Among the 10 habitat predictors, three variables (mid‐river depth, river width, and water temperature) were significantly correlated (p <.05) with the probability of Gharial occurrence. The model shows that Gharial detection probability increases with greater mid‐river depth and width and lower water temperature. This study establishes a population baseline for Gharials within the river system before the construction of large infrastructure projects, such as dams and irrigation canals. It also recommends continuous monitoring of Gharial populations after water release and/or diversion to evaluate the impact of large infrastructure projects on the population and their associated habitat characteristics. This will help enable more informed and targeted conservation efforts. [ABSTRACT FROM AUTHOR]

Key Clinical Message: The report urges considering rare neoplastic tumor, like IMT, in intussusception cases and underscores the vital role of comprehensive and swift diagnosis in influencing treatment choices and patient results. We present a case of a 36‐year‐old male who presented with abdominal pain and vomiting. Inflammatory markers were elevated in routine investigations, while other laboratory parameters were within normal limits. Ultrasound imaging revealed a target lesion, which required further evaluation with a CT scan and confirmed ileocolic intussusception. An exploratory laparotomy showed a solid lesion measuring 5 by 6 cm in the cecum, along with evidence of ileocolic intussusception. A standard right hemicolectomy was performed, and the postoperative course was uneventful. Histopathological examination showed an inflammatory myofibroblastic tumor with nodular hyperplasia. The report highlighted the importance of assessing rare neoplastic causes in patients with intussusception. [ABSTRACT FROM AUTHOR]

Novel approaches are required to improve the efficacy of immunotherapies and increase the proportion of patients who experience a benefit. Antibody‐dependent cell‐mediated cytotoxicity (ADCC) contributes to the efficacy of many monoclonal antibodies therapies. Natural killer (NK) cells mediate ADCC, though responses are highly variable and depend on prior treatment as well as other factors. Thus, strategies to increase NK cell activity are expected to improve multiple therapies. Both cytokine treatment and NK cell receptor engineering are being explored to increase ADCC. Post‐translational modifications, including glycosylation, are widely recognized as mediators of cellular processes but minimally explored as an alternative strategy to increase ADCC. We evaluated the impact of treatment with kifunensine, an inhibitor of asparagine‐linked (N‐)glycan processing, on ADCC using primary and cultured human NK cells. We also probed affinity using binding assays and CD16a structure with nuclear magnetic resonance spectroscopy. Treating primary human NK cells and cultured YTS‐CD16a cells with kifunensine doubled ADCC in a CD16a‐dependent manner. Kifunensine treatment also increased the antibody‐binding affinity of CD16a on the NK cell surface. Structural interrogation identified a single CD16a region, proximal to the N162 glycan and the antibody‐binding interface, perturbed by the N‐glycan composition. The observed increase in NK cell activity following kifunensine treatment synergized with afucosylated antibodies, further increasing ADCC by an additional 33%. These results demonstrate native N‐glycan processing is an important factor that limits NK cell ADCC. Furthermore, optimal antibody and CD16a glycoforms are defined that provide the greatest ADCC activity. [ABSTRACT FROM AUTHOR]

In Nepal, live donor organ transplantation is only 14 years old with the first successful kidney transplant made in 2008 and a successful liver and bone marrow transplant made in 2016. However, transplantation of cadaveric cornea dates back to 1998. There are still no cases of animal‐to‐human organ transplantation in Nepal. There are stringent laws to regulate human body organ transplantation in Nepal which are amended from time to time. However, there is a racket of human traffickers who lure rural people from this low‐income country into the illegal organ trade. Furthermore, there is a substantial lack of awareness of organ donation among the general public. This article focuses on the stipulations of ethical, legal, and practical issues of obtaining organs procured from living and brain‐dead donors that support the process of transplantation in Nepal. In addition, the article also explores the legal and practical issues of organ trafficking and organ donation awareness in Nepal on the basis of factual data and findings from other studies. [ABSTRACT FROM AUTHOR]

People with silicosis may develop Erasmus syndrome, a condition characterized by the emergence of systemic sclerosis (SSc) after silica exposure. This case study emphasizes the significance of understanding the connection between occupational silica exposure, silicosis, and SSc. A 24‐year‐old male stonecutter got silicosis and a form of SSc following 8 years on his job as a stonecutter. The signs and symptoms the patient experienced were Raynaud's phenomenon, cutaneous fibrosis, arthralgia, digital pitting, and respiratory distress. High‐resolution computed tomography (HRCT) revealed interstitial lung disease and calcified mediastinal lymph nodes. This case study demonstrates the clinical importance of the relationship between occupational silica exposure, silicosis, SSc, and Erasmus syndrome. Healthcare providers need to be aware of the possible difficulties and issues that may result from silica exposure. They should prioritize quick detection and efficient treatment plans for those who have been exposed to silica while on the job. [ABSTRACT FROM AUTHOR]

Key Clinical Message: Idiopathic Castleman disease transforming into Diffuse Large B‐cell Lymphoma has an aggressive course and can lead to mortality. Hence, early diagnosis and intervention are required. Idiopathic Castleman disease transforming into non‐Hodgkin lymphoma has an aggressive course, poor prognosis, and high mortality rate. Hence, early diagnosis and intervention are necessary. In a developing country like Nepal, where infectious diseases, particularly TB, are high, concomitant infection worsens the disease course. It also poses a diagnostic challenge as the clinical presentation may be similar. [ABSTRACT FROM AUTHOR]

Since its first demonstration over 100 years ago, scattering‐based light‐sheet microscopy has recently re‐emerged as a key modality in label‐free tissue imaging and cellular morphometry; however, scattering‐based light‐sheet imaging with subcellular resolution remains an unmet target. This is because related approaches inevitably superimpose speckle or granular intensity modulation on to the native subcellular features. Here, we addressed this challenge by deploying a time‐averaged pseudo‐thermalized light‐sheet illumination. While this approach increased the lateral dimensions of the illumination sheet, we achieved subcellular resolving power after image deconvolution. We validated this approach by imaging cytosolic carbon depots in yeast and bacteria with increased specificity, no staining, and ultralow irradiance levels. Overall, we expect this scattering‐based light‐sheet microscopy approach will advance single, live cell imaging by conferring low‐irradiance and label‐free operation towards eradicating phototoxicity. [ABSTRACT FROM AUTHOR]

Key Clinical Message: Disseminated tuberculosis (TB) resulting from lymphohematogenous dissemination of Mycobacterium tuberculosis during primary infection or reactivation of latent disease is rare among young immunocompetent patients. Central nervous system TB (CNS TB) is one of the most challenging clinical diagnoses with high fatality. Here, we describe a young immunocompetent female with no known comorbidities initially presented with military pulmonary TB and later developed CNS TB. This presentation of disseminated TB in immunocompetent patient warrant early diagnosis and treatment. [ABSTRACT FROM AUTHOR]