13 results on '"Stejskal V"'
Search Results
2. Splenic lipidosis in intensively cultured perch, Perca fluviatilis L.
- Author
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Stejskal, V, Kouřil, J, Policar, T, and Svobodová, Z
- Subjects
- *
LIPIDOSES , *EUROPEAN perch , *FISH farming , *LIPID metabolism disorders , *FISH mortality , *FISH diseases - Abstract
Macroscopically visible lipid deposition varying in size from pinpoint to 8-mm diameter was found in spleens of a population of intensively farmed perch, Perca fluviatilis L. over a 24-month rearing period. Large agglomerates of adipocytes distinguishable from surrounding normal tissue occurred in all individuals with spleen lipidosis. Several affected fish presented total dystrophy of large clusters of hepatocytes. Prevalence of lipidosis was 5.0% at 12 months and 16.6% at 24 months. There was no significant difference between fatty acid profiles of liver or perivisceral fat of perch with and without lipidosis except for linoleic, myristic, c-linoleic, cis-eicosatrienic, palmitooleic acid. Body weight and hepatosomatic, perivisceral fat and splenosomatic indices were not associated with lipidosis. There was no significant effect of lipidosis on mortality or growth. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
3. Large-scale mating disruption of Ephestia spp. and Plodia interpunctella in Czech Republic, Greece and Italy.
- Author
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Trematerra, P., Athanassiou, C., Stejskal, V., Sciarretta, A., Kavallieratos, N., and Palyvos, N.
- Subjects
ANIMAL courtship ,EPHESTIA ,INDIANMEAL moth ,PHEROMONES ,FIELD research - Abstract
Field trials were carried out to evaluate the use of the pheromone (9 Z,12 E)-tetradecadienyl acetate (TDA/ZETA) for mating disruption (MD) of Pyralidae moths associated with stored products, in most cases the Raisin moth, Ephestia cautella (Walker), Mediterranean flour moth, Ephestia kuehniella Zeller and Indianmeal moth, Plodia interpunctella (Hübner). The experiments were conducted in the Czech Republic, Greece and Italy during 2007 and 2008 in storage facilities that varied in their size and type, and included flour mills, retail stores, storage rooms with currants and raw grain stores. After a summer pre-treatment monitoring period to assess moth population in, dispensers containing TDA were placed in the fall. Adjacent facilities without dispensers were used as control units. Pheromone-baited traps were used to monitor the population fluctuation of the pyralid moths during the entire experimental periods. The presence of MD dispensers notably reduced the number of adults found in the traps in comparison with control rooms. Monitoring of female oviposition, measured as number of hatched larvae in cups containing food, indicated that there was a reduction in the number of larvae in the areas with MD dispensers. The results of the present work indicate that the use of mating disruption is feasible against pyralid moths in storage facilities, and should be further evaluated as a component of an integrated pest management based control strategy. [ABSTRACT FROM AUTHOR]
- Published
- 2011
- Full Text
- View/download PDF
4. Computer-based image analysis to quantify the number of micro-arthropods in a sample.
- Author
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Lukas, J., Kucerova, Z., and Stejskal, V.
- Subjects
IMAGE analysis ,ARTHROPODA ,IMAGING systems ,INVERTEBRATES ,ANIMALS - Abstract
The article offers information on digital image analysis (DIA) for the quantification of the number of micro-arthropods in a sample. It presents a comparison with traditional direct visual counting using Acarus siro L. and Liposcelis bostrychophila Badonnel. For the analysis, the SigmaScan Pro 5 software was used.
- Published
- 2009
- Full Text
- View/download PDF
5. Combined effect of an antifeedant α-amylase inhibitor and a predator Cheyletus malaccensis in controlling the stored-product mite Acarus siro.
- Author
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HUBERT, J., HÝBLOVÁ, J., MÜNZBERGOVÁ, Z., PEKÁR, S., KŘÍŽKOVA-KUDLÍKOVÁ, I., DOLEČKOVÁ-MAREŠOVÁ, L., STEJSKAL, V., and MAREŠ, M.
- Subjects
ACARUS siro ,INSECT antifeedants ,AMYLASE inhibitors ,PREDATION ,TOXIGENIC fungi ,ACARBOSE ,PHYSIOLOGICAL control systems - Abstract
Acarus siro is the most abundant and frequent mite to infest stored-food products, causing allergies and transmitting mycotoxin producing fungi. The predatory mite Cheyletus malaccensis is a candidate species in the biocontrol programme for this pest. In vitro, the α-amylase inhibitor acarbose is effective against the α-amylase of A. siro but not against that of C. malaccensis. In vivo, the impact of acarbose on a population of A. siro is investigated along with the interaction with the predator. Various densities of adult parthenogenetic females of C. malaccensis are reared on A. siro feeding on either a control diet or a diet containing different acarbose concentrations. The combined action of both factors significantly improved the final biocontrol efficiency with C. malaccensis, compensating for the lower energetic content of the prey on acarbose by increasing the number of prey caught. Acarbose had no negative effects on the longevity and the length of oviposition period of C. malaccensis but partially reduced its fecundity. The results are discussed in the context of the integrated control of stored-product mite pests. [ABSTRACT FROM AUTHOR]
- Published
- 2007
- Full Text
- View/download PDF
6. In vitro lymphocyte proliferation in the diagnosis of allergy to phenoxymethylpenicillin.
- Author
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Cederbrant, K., Stejskal, V., Broman, P., Lindkvist, L., and Sundell, K.
- Subjects
SIDE effects of antibiotics ,LYMPHOCYTES ,LYMPHOCYTE transformation ,DRUG allergy ,ALLERGIES - Abstract
Background The aim of this study was to investigate in vitro lymphocyte proliferation in the diagnosis of allergy to phenoxymethylpenicillin (PcV), comparing chemically reactive PcV, added to cell cultures in unconjugated form, to a PcV-PLL (poly-L-lysine) conjugate as antigens. Side-chain specificity of lymphoproliferative responses was investigated with reactive benzylpenicillin (PcG) and bacampicillin. Methods Seventeen patients with a history of hypersensitivity reactions in connection with PcV treatment were studied by means of the lymphocyte transformation test (LTT), the radioallergosorbent test (RAST). skin tests (prick and intracutaneous), and oral challenge with PcV. LTT was also performed in 20 control subjects exposed to PcV therapeutically, and in eight subjects with occupational exposure to this penicillin. Results Nine patients had a positive in vivo test to PcV (five by oral challenge, three by intracutaneous test, and one by both tests), and six were challenge-negative. When reactive PcV was used as antigen in LTT, positive LTT responses were observed in five of the nine patients with a positive in vivo test, and two of them were also side-chain specific. Positive LTT responses with reactive PcV also correlated with a positive RAST in five of seven subjects. None of the six patients with a negative challenge test, and only one of the 28 controls showed a positive LTT result with reactive PcV. Thus, the specificity of LTT with reactive PcV was 96%. In contrast, when PLL-conjugated PcV served as antigen, four challenge-negative subjects and 11 controls were LTT-positive. Conclusions The results of this study indicate that LTT with chemically reactive PcV could be useful as an in vitro complement in the diagnosis of PcV allergy and as a tool to reveal the side-chain specificity of peripheral blood lymphocytes. A positive LTT to PLL-conjugated PcV may be an indicator of immunization, but not necessarily allergy, to the penicilloyl structure. [ABSTRACT FROM AUTHOR]
- Published
- 1998
- Full Text
- View/download PDF
7. An analysis of β-lactam-derived antigens on spleen cell and serum proteins by ELISA and Western blotting.
- Author
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Warbrick, E. V., Thomas, A. L., Stejskal, V., and Coleman, J. W.
- Subjects
PENICILLIUM ,ANTIBIOTICS ,ANTIGENS ,BLOOD proteins ,PENICILLIN ,CEPHALOSPORINS - Abstract
Penicillins and related beta-lactam antibiotics are known to conjugate to proteins to generate potentially antigenic (haptenic) determinants. In the present study, we used a rabbit polyclonal antibody raised against benzylpenicillin (BP) to investigate the capacity of six penicillins and one cephalosporin to generate haptenic groups in vitro on cultured mouse spleen cells and on serum proteins in the culture medium. All of the drugs tested, namely, BP, amoxicillin (AMX), ampicillin (AMP), cephalothin (CEP), cloxacillin (CLX), flucloxacillin (FLX), and phenoxymethylpenicillin (PMP) generated antigens in a concentration-dependent manner on cell and serum proteins, which could be detected by ELISA, although antigens generated by BP, CEP, FLX, or PMP in either cell- or serum-conjugated form were more readily detected than those generated by AMX, AMP, or CLX. Western blot analysis revealed that BP-derived antigens were generated relatively slowly on cell proteins (maximum binding was not yet reached after 8 h), compared to serum proteins (maximum binding within 1 h). BP, CEP, and PMP all generated similar distinctive patterns of immunostaining of electrophoresed cell or serum proteins which did not reflect the relative abundance of different proteins as revealed by Coomassie brilliant blue staining. FLX, CLX, AMP, and AMX did not generate antigens that could be detected on Western blots. In conclusion, we have shown that various beta-lactam antibiotics generate antigens on cell and serum proteins that can be detected and characterized immunochemically with polyclonal antiserum. Further application of these methods may offer potential for further identification of immunologically relevant cellular and serum antigens generated by these drugs. [ABSTRACT FROM AUTHOR]
- Published
- 1995
- Full Text
- View/download PDF
8. Absence of specific IgE antibodies in allergic contact sensitivity to formaldehyde.
- Author
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Lidén, S., Scheynius, A., Fischer, T., Johansson, S. G. O., Ruhnek-Forsbeck, M., and Stejskal, V.
- Subjects
IMMUNOLOGY ,IMMUNOGLOBULIN E ,IMMUNOGLOBULINS ,FORMALDEHYDE ,CONTACT dermatitis ,DELAYED hypersensitivity - Abstract
Immunologic reactions are customarily divided into two broad categories, cell-mediated and antibody-mediated. An interplay between these two pathogenetic principles is indicated by reactions such as cutaneous basophil hypersensitivity, late-phase reaction, and cutaneous lesions indistinguishable from regular allergic contact dermatitis lesions after sensitization with IgE antibodies against certain haptens. In the present study, 23 patients with a history of a positive epicutaneous test to formaldehyde participated. On retest, 15 showed a positive reaction. Eight patients were Phadiatop® positive, indicating an atopic diathesis, and eight had a history of or ongoing atopic dermatitis. On RAST test®, only two, nonatopic patients had specific IgE antibodies to formaldehyde. In the cellular infiltrates of biopsies from epicutaneous test sites, cells reactive with monoclonal antibodies against IgE were found in positive and negative formalin tests, both in atopics and nonatopics, as well as in control biopsies from nonlesional skin. Double immunofluorescence staining experiments showed that IgE occurred on Langerhans' cells. The proportion of IgE-positive cells correlated to the level of serum IgE, but not to atopy. These cells were also found both in the epidermis and in the dermis in nonatopic patients. ICAM-1 occurred on keratinocytes in all patient groups. This study does not support the hypothesis that specific IgE antibodies are active in the pathogenesis of contact sensitivity to formaldehyde either in atopic or in nonatopic patients. [ABSTRACT FROM AUTHOR]
- Published
- 1993
- Full Text
- View/download PDF
9. The effect of grain size on the biology of Sitophilus granarius L. (Col., Curculionidae). I. Oviposition, distribution of eggs and adult emergence.
- Author
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Stejskal, V. and Kučerová, Z.
- Subjects
GRANARY weevil ,OVIPARITY ,GRAIN ,INSECT eggs ,INSECT population density ,EMBRYOLOGY ,WHEAT ,GRANIVORES ,BIOLOGY - Abstract
The oviposition and rate of emergence of Sitophilus granarius in two extreme sizes of wheat kernels was studied under 4 levels of population density. The behaviour of S. granarius was considered to be maladaptive, as in all tested population densities the zero level of aggregation was never recorded. With increasing number of grains available the aggregation of eggs decreased. In the mixed-size samples large kernels were infested more frequently than small. However, the ratio of eggs laid on large versus small kernels was positively density dependent. In higher population densities, a higher load of eggs and adults per gramme of small grains was recorded. The importance of grain size, and of oviposition, in internally feeding granivores is discussed. [ABSTRACT FROM AUTHOR]
- Published
- 1996
- Full Text
- View/download PDF
10. Differential Cytotoxicity of Activated Lymphocytes on Allogeneic and Xenogeneic Target Cells IV. Competitive Inhibition of Target Cell Lysis by Addition of Unlabeled Cells.
- Author
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Stejskal, V.
- Subjects
LYMPHOCYTES ,CELLS ,URINARY organs ,CHROMIUM ,BLADDER ,KIDNEYS - Abstract
A competitive inhibition assay was used to define the specificity of the in vitro cytotoxicity of activated lymphocytes. Human and mouse effector cells were produced by prestimulation with phytohtmagglutinin, pokeweed mitogen, or purified protein derivative for several days. The addition of increasing numbers of unlabeled Chang cells to a fixed number of stimulated human lymphocytes and
51 Cr-labeled Chang cells gradually decreased chromium release. Admixture of unlabeled human bladder tumor cells, lung cells, or monkey kidney cells had a similar effect, whereas mouse L cells were not inhibitory. The reverse was found to be true when stimulated mouse lymphocytes and51 Cr-labeled mouse L cells were incubated. In this case, the addition of unlabeled L or rat tumor cells effectively inhibited51 Cr release, whereas human or monkey cells were less inhibitory. Both human and mouse cells inhibited the cytotoxicity of human effector lymphocytes towards mouse L cells. The cytotoxic effects of mouse lymphocytes on human cells were also decreased by addition of unlabeled human, monkey, or mouse cells, but in this case human cells were most inhibitory. The results indicate that activated lymphocytes recognize several surface structures on the target cells. Some of these structures may be shared by cells of different species origin, whereas others seem to be unique for cells from phylogenetically closely related species. [ABSTRACT FROM AUTHOR]- Published
- 1976
- Full Text
- View/download PDF
11. Long-term changes of cockroach infestations in Czech and Slovak food-processing plants.
- Author
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STEJSKAL, V. and VERNER, P. H.
- Published
- 1996
- Full Text
- View/download PDF
12. The effect of grain size on the biology of Sitophilus granarius L. (Col., Curculionidae). 1. Oviposition, distribution of eggs and adult emergence
- Author
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Stejskal, V. and Kucerova, Z.
- Subjects
GRANARY weevil ,POPULATION density ,WHEAT - Published
- 1996
- Full Text
- View/download PDF
13. An analysis of beta-lactam-derived antigens on spleen cell and serum proteins by ELISA and Western blotting.
- Author
-
Warbrick EV, Thomas AL, Stejskal V, and Coleman JW
- Subjects
- Animals, Blotting, Western, Cells, Cultured, Drug Hypersensitivity immunology, Enzyme-Linked Immunosorbent Assay, Epitopes analysis, Female, Mice, Penicillin G immunology, Rabbits, Spleen cytology, Antigens analysis, Blood Proteins immunology, Cephalosporins immunology, Penicillins immunology, Spleen immunology
- Abstract
Penicillins and related beta-lactam antibiotics are known to conjugate to proteins to generate potentially antigenic (haptenic) determinants. In the present study, we used a rabbit polyclonal antibody raised against benzylpenicillin (BP) to investigate the capacity of six penicillins and one cephalosporin to generate haptenic groups in vitro on cultured mouse spleen cells and on serum proteins in the culture medium. All of the drugs tested, namely, BP, amoxicillin (AMX), ampicillin (AMP), cephalothin (CEP), cloxacillin (CLX), flucloxacillin (FLX), and phenoxymethylpenicillin (PMP) generated antigens in a concentration-dependent manner on cell and serum proteins, which could be detected by ELISA, although antigens generated by BP, CEP, FLX, or PMP in either cell- or serum-conjugated form were more readily detected than those generated by AMX, AMP, or CLX. Western blot analysis revealed that BP-derived antigens were generated relatively slowly on cell proteins (maximum binding was not yet reached after 8 h), compared to serum proteins (maximum binding within 1 h). BP, CEP, and PMP all generated similar distinctive patterns of immunostaining of electrophoresed cell or serum proteins which did not reflect the relative abundance of different proteins as revealed by Coomassie brilliant blue staining. FLX, CLX, AMP, and AMX did not generate antigens that could be detected on Western blots. In conclusion, we have shown that various beta-lactam antibiotics generate antigens on cell and serum proteins that can be detected and characterized immunochemically with polyclonal antiserum. Further application of these methods may offer potential for further identification of immunologically relevant cellular and serum antigens generated by these drugs.
- Published
- 1995
- Full Text
- View/download PDF
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