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INTRODUCTION: Limited observational windows lead to conflicting results in studies examining educational differences in Alzheimer's disease and related dementias (ADRD) risk, due to observational window bias relative to onset of accelerated cognitive decline. This study tested a novel model to address observational window bias and tested for the presence and sources of disparities in accelerated cognitive declines due to ADRD. METHODS: The sample examined 167,314 cognitive assessments from 32,441 Health and Retirement Study participants. We implemented a parametric non‐linear nested longitudinal regression and reported multivariable‐adjusted nodal incidence ratios (aNIR). RESULTS: University degrees were associated with lower incidence (aNIR = 0.253, 95% confidence interval [CI] = [0.221 to 0.289], p < 0.001), while black participants had a higher incidence (aNIR = 1.995, [1.858 to 2.141], p < 0.001) of accelerated cognitive decline, adjusting for demographic, sociobehavioral, and medical risk factors. Sex‐stratified analyses identified diminished educational returns for women and increased incidence among minoritized women. DISCUSSION: Addressing observational window bias reveals large social inequalities in the onset of accelerated cognitive declines indicative of ADRD. Highlights: This study identifies observational window bias as a source of conflicting results among previous studies of educational achievement in Alzheimer's disease and related dementias (ADRD) disparities.The study locates preclinical accelerated cognitive decline, which is indicative of ADRD while occurring 10+ years prior to symptom onset, as a site to study ADRD disparities that mitigates observational window bias.A novel method, nested non‐linear regression, is developed to test for differences in the onset of accelerated cognitive decline.Educational and racial/ethnic disparities are demonstrated in the onset of accelerated cognitive decline, as are their intersecting differences with sex/gender. [ABSTRACT FROM AUTHOR]

Background and Objectives: Pulse flours are gaining popularity in baked goods, but their use is limited due to strong flavors. This study analyzed the addition of pea flour to pancakes, with and without sodium metabisulfite (SMB), to assess their impact on pancake quality and reduce off‐flavors. Findings: Because pancake formulations were standardized based on batter viscosity, only at 20% pea flour incorporation did changes to pancake physical quality occur. Adding pea flour to pancakes impacted sensory aspects such as cooked pea flavor, nutty flavor, and pea odor. In combination with SMB, pea flour can be added up to 5% without imparting significant cooked pea flavor to pancakes. SMB also reduced pea odor in pancakes with 1%–5% pea flour. Conclusions: Pea flour can be added to pancakes without significantly affecting physical quality, as long as the batter viscosity is optimized. SMB can have a positive effect on limiting off‐flavors in pancakes containing pea flour. This research provides valuable insights into the effects of substituting pea flour in soft wheat batter‐based bake systems. Significance and Novelty: Pancakes with up to 20% pea flour had similar physical characteristics to wheat pancakes. Pea flavors were detectible at 5% inclusion, and SMB reduced off‐flavor perception at low pea flour inclusion rates. [ABSTRACT FROM AUTHOR]

Meta‐analyses have become a valuable tool with which to synthesize effects across studies, but in ecology and evolution, they are often characterized by high heterogeneity, where effect sizes vary between studies. Much of this heterogeneity can be attributed to species‐specific differences in responses to predictor variables. Here, we aimed to incorporate a novel trait‐based approach to explain species‐specific differences in a meta‐analysis by testing the ability of morphological traits to explain why the effectiveness of flight‐intercept trap design varies according to beetle species, a critical issue in forest pest management. An existing morphological trait database for forest beetles was supplemented, providing trait data for 97 species, while data from a previous meta‐analysis on capture rates of bark or woodboring beetles according to different trap designs were updated. We combined these sources by including nine morphological traits as moderators in meta‐analysis models, for five different components of trap design. Traits were selected based on theoretical hypotheses relating to beetle movement, maneuverability, and sensory perception. We compared the performance of morphological traits as moderators versus guild, taxonomic family, and null meta‐analysis models. Morphological traits for the effect of trap type (panel vs. multiple‐funnel) on beetle capture rates improved model fit (AICc), reduced within‐study variance (σ2), and explained more variation (McFadden's pseudo‐R2) compared with null, guild, and taxonomic family models. For example, morphological trait models explained 10% more of the variance (pseudo‐R2) when compared with a null model. However, using traits was less informative to explain how detailed elements of trap design such as surface treatment and color influence capture rates. The reduction of within‐study variance when accounting for morphological traits demonstrates their potential value for explaining species‐specific differences. Morphological traits associated with flight efficiency, maneuverability, and eye size were particularly informative for explaining the effectiveness of trap type. This could lead to improved predictability of optimal trap design according to species. Therefore, morphological traits could be a valuable tool for understanding species‐specific differences in community ecology, but other causes of heterogeneity across studies, such as forest type and structure, require further investigation. [ABSTRACT FROM AUTHOR]

The Sacramento–San Joaquin Delta (hereafter, "the Delta") is one of the estuaries with the most invasive species in the world, and nonnative predators may be a major factor in the observed decline of Central Valley Chinook Salmon Oncorhynchus tshawytscha over recent decades. In order for managers to take actions that might reduce predation‐related mortality for these ecologically, culturally, and economically valuable fish, it is important to understand the factors influencing the distribution and abundance of piscivores in the Delta. In this study, we used a dual‐frequency identification sonar (i.e., DIDSON) to conduct mobile surveys to quantify the abundances of piscivores in the Delta. We then used these data to identify the habitat features that are correlated with the abundance of piscivores. Prior to conducting the surveys, we used DIDSON data from captured fish to develop an algorithm to distinguish piscivores from nonpiscivores with high confidence (98% accuracy). A generalized linear mixed‐effects model fit to these survey data indicated that predator abundances were most associated with areas of increased submerged aquatic vegetation patches, and channels that are straighter, with increased bathymetric complexity. When applied to the entire survey area, this model was successfully able to predict known areas of high predator densities. These results indicate that one approach to reduce predator densities in key locations throughout the Delta, and improve juvenile salmonid outmigration survival, is to reduce the extent of invasive submerged aquatic vegetation. Because experimental predator removals have been largely ineffective in the Delta, efforts to manipulate habitat to discourage nonnative predator recruitment and favor native species recruitment may provide a more effective solution to improve salmonid survival rates. [ABSTRACT FROM AUTHOR]

This consensus guidance statement reflects input from patient communities and the authors thank the following organizations and individuals for their input during the Collaborative writing process: the Patient-Led Research Collaborative, Angela Meriquez Vazquez, MSW, Long Covid Patient and Advocate, and Lauren Nichols, Long Covid Patient and Chronic Illness Advocate. For example, patients with MS may be on disease modifying therapy (DMT), and both the MS and the DMT may put them at higher risk for COVID-19 acute infections as well as more severe course, though in a recent systematic review these were not consistent findings.58 The review included more than 80 reports involving 2493 MS patients and 37 Neuromyelitis Optica Spectrum Disorder patients with COVID-19. Larger number of beds and location in an area with high COVID-19 prevalence were the strongest and most consistent predictors of facilities having more COVID-19 cases and deaths.56 This multicenter cohort study showed that delirium was the sixth most common of all presenting symptoms and signs of acute COVID-19 infection, and factors associated with delirium were age older than 75 years, living in a nursing home or assisted living, vision impairment, hearing impairment, stroke, and Parkinson disease.57Though the studies in children are evolving, long COVID is recognized and should be considered at all ages. Patients with GBS/MFS benefit further from intravenous immunoglobulin and plasma exchange.[7] Most patients with isolated cranial neuropathies have a favorable outcome with supportive care compared to patients with multiple cranial neuropathies with GBS, who may achieve partial recovery and experience longer symptom duration. [Extracted from the article]

Historically, the Monongahela, Tygart, and Cheat River watersheds in West Virginia were impaired by acidification from acid mine drainage and Walleye Sander vitreus were extirpated from these watersheds by the 1940s. Walleye were reestablished after water quality improvements following passage of environmental legislation and subsequent reintroduction efforts. We compared population characteristics, with emphasis on growth, of Walleye and used modeling to predict the potential effects of harvest regulations in the Monongahela River and two main‐stem reservoirs in the Cheat River and Tygart River watersheds. Statistical comparisons of von Bertalanffy growth curves and relative growth indices indicated that Walleye growth significantly differed across all water bodies. Relative growth index results suggested that Walleye growth was above average in Cheat Lake, average in the Monongahela River, and below average in Tygart Lake relative to other North American populations. Growth was negatively correlated with Walleye relative abundance and positively correlated with estimates of productivity (total phosphorus, chlorophyll a). Walleye diets significantly differed across all water bodies, with diets dominated by Yellow Perch Perca flavescens and Gizzard Shad Dorosoma cepedianum in Cheat Lake, where growth was fastest. Population modeling suggested that effects of exploitation on yield, spawning potential, and size structure were similar under regulations of no length limit and a minimum length limit (381 mm). Models suggested that removing length limits in Tygart Lake could increase angler harvest opportunities and pose minimal threat to the fishery. Models suggested that a protected slot limit could provide increased protection to the spawning potential of Cheat Lake and the Monongahela River populations. Additionally, models predicted that a protected slot limit could increase the number of large (>630‐mm) Walleye in these waters. Our findings demonstrate the different characteristics that Walleye populations can develop after reestablishment based on abiotic and biotic conditions and the need for watershed‐specific management. [ABSTRACT FROM AUTHOR]

Recent foodborne illness outbreaks have heightened pressures on growers to deter wildlife from farms, jeopardizing conservation efforts. However, it remains unclear which species, particularly birds, pose the greatest risk to food safety. Using >11,000 pathogen tests and 1565 bird surveys covering 139 bird species from across the western United States, we examined the importance of 11 traits in mediating wild bird risk to food safety. We tested whether traits associated with pathogen exposure (e.g., habitat associations, movement, and foraging strategy) and pace‐of‐life (clutch size and generation length) mediated foodborne pathogen prevalence and proclivities to enter farm fields and defecate on crops. Campylobacter spp. were the most prevalent enteric pathogen (8.0%), while Salmonella and Shiga‐toxin producing Escherichia coli (STEC) were rare (0.46% and 0.22% prevalence, respectively). We found that several traits related to pathogen exposure predicted pathogen prevalence. Specifically, Campylobacter and STEC‐associated virulence genes were more often detected in species associated with cattle feedlots and bird feeders, respectively. Campylobacter was also more prevalent in species that consumed plants and had longer generation lengths. We found that species associated with feedlots were more likely to enter fields and defecate on crops. Our results indicated that canopy‐foraging insectivores were less likely to deposit foodborne pathogens on crops, suggesting growers may be able to promote pest‐eating birds and birds of conservation concern (e.g., via nest boxes) without necessarily compromising food safety. As such, promoting insectivorous birds may represent a win‐win‐win for bird conservation, crop production, and food safety. Collectively, our results suggest that separating crop production from livestock farming may be the best way to lower food safety risks from birds. More broadly, our trait‐based framework suggests a path forward for co‐managing wildlife conservation and food safety risks in farmlands by providing a strategy for holistically evaluating the food safety risks of wild animals, including under‐studied species. [ABSTRACT FROM AUTHOR]

Rapid environmental change is reshaping ecosystems and driving species loss globally. Carnivore populations have declined and retracted rapidly and have been the target of numerous translocation projects. Success, however, is complicated when these efforts occur in novel ecosystems. Identifying refuges, locations that are resistant to environmental change, within a translocation framework should improve population recovery and persistence. American martens (Martes americana) are the most frequently translocated carnivore in North America. As elsewhere, martens were extirpated across much of the Great Lakes region by the 1930s and, despite multiple translocations beginning in the 1950s, martens remain of regional conservation concern. Surprisingly, martens were rediscovered in 2014 on the Apostle Islands of Lake Superior after a putative absence of >40 yr. To identify the source of martens to the islands and understand connectivity of the reintroduction network, we collected genetic data on martens from the archipelago and from all regional reintroduction sites. In total, we genotyped 483 individual martens, 43 of which inhabited the Apostle Islands (densities 0.42–1.46 km−2). Coalescent analyses supported the contemporary recolonization of the Apostle Islands with progenitors likely originating from Michigan, which were sourced from Ontario. We also identified movements by a first‐order relative between the Apostle Islands and the recovery network. We detected some regional gene flow, but in an unexpected direction: individuals moving from the islands to the mainland. Our findings suggest that the Apostle Islands were naturally recolonized by progeny of translocated individuals and now act as a source back to the reintroduction sites on the mainland. We suggest that the Apostle Islands, given its protection from disturbance, complex forest structure, and reduced carnivore competition, will act as a potential refuge for marten along their trailing range boundary and a central node for regional recovery. Our work reveals that translocations, even those occurring along southern range boundaries, can create recovery networks that function like natural metapopulations. Identifying refuges, locations that are resistant to environmental change, within these recovery networks can further improve species recovery, even within novel environments. Future translocation planning should a priori identify potential refuges and sources to improve short‐term recovery and long‐term persistence. [ABSTRACT FROM AUTHOR]

Background: Individuals with spinal cord injuries and disorders (SCI/D) require frequent interdisciplinary health care to address impairments in mobility, autonomic function, and secondary complications. Telehealth has the capacity to substantially transform health care delivery and improve care by increasing access and communication. However, relatively little is known about telehealth use in this specific population. Here we attempt to fill part of this gap. Objective: To investigate the frequency and characteristics associated with telehealth use in Veterans with SCI/D. Design: Cross‐sectional, descriptive project. Setting: Veterans Health Administration (VHA) facilities. Participants: A total of 15 028 Veterans living with SCI/D who received services from the VHA SCI/D System of Care. Intervention: Not applicable. Outcome Measures: Frequency and characteristics associated with VHA telehealth utilization. Results: Of the 15 028 Veterans with SCI/D included in the evaluation, 17% used some form of telehealth in VHA Fiscal Year (FY)2017. Veterans older than 65 years of age had lower odds (odds ratio [OR] = 0.88, P <.05, confidence interval [CI] 0.80‐0.98) of using telehealth. Being Caucasian (OR = 1.29, P <.01, CI 1.09‐1.52), living in rural areas (OR = 1.16, P <.01, CI 1.05‐1.28), living greater distances away from the VHA (P <.01 for all distances), and being in priority group 8, meaning that Veterans have higher copayment requirements (OR = 1.46, P <.001, CI 1.19‐1.81), were all significantly associated with greater odds of telehealth use. The most frequent types of telehealth used were real‐time clinical video and store‐and‐forward between a provider and patient within the same hub network. Conclusion: There are opportunities to increase telehealth adoption in the SCI/D arena. The findings from this project highlight which Veterans are currently using telehealth services, as well as gaps regarding telehealth adoption in this population. [ABSTRACT FROM AUTHOR]

Channel Catfish Ictalurus punctatus were collected during 2018 and 2019 from the Monongahela River in West Virginia using hoop nets to estimate population dynamics. Length, weight, sex, fecundity, and age data were obtained from collected individuals. Population characteristics (e.g., relative abundance, size structure, age structure, growth, etc.) were modeled under three proposed length limits of 300, 375, and 450 mm. Simulation results indicated that growth overfishing may occur when exploitation rates reach 36% under a 300‐mm minimum length limit. Furthermore, recruitment overfishing began to occur at 40% exploitation under a 300‐mm minimum length limit. Additionally, simulations suggest that no recruitment or growth overfishing occur at minimum length limits of 375 mm or 450 mm until extremely high exploitation levels (>90%) are reached. Thus, future consideration should be given for implementation of a 375‐mm minimum length limit of Channel Catfish in the Monongahela River to prevent growth and recruitment overfishing while also ensuring anglers are still able to harvest Channel Catfish. [ABSTRACT FROM AUTHOR]

Rhabdomyosarcoma (RMS) is an aggressive pediatric soft tissue sarcoma. There are two main subtypes of RMS, alveolar rhabdomyosarcoma (ARMS) and embryonal rhabdomyosarcoma. ARMS typically encompasses fusion‐positive rhabdomyosarcoma, which expresses either PAX3‐FOXO1 or PAX7‐FOXO1 fusion proteins. There are no targeted therapies for ARMS; however, recent studies have begun to illustrate the cooperation between epigenetic proteins and the PAX3‐FOXO1 fusion, indicating that epigenetic proteins may serve as targets in ARMS. Here, we investigate the contribution of BMI1, given the established role of this epigenetic regulator in sustaining aggression in cancer. We determined that BMI1 is expressed across ARMS tumors, patient‐derived xenografts, and cell lines. We depleted BMI1 using RNAi and inhibitors (PTC‐209 and PTC‐028) and found that this leads to a decrease in cell growth/increase in apoptosis in vitro, and delays tumor growth in vivo. Our data suggest that BMI1 inhibition activates the Hippo pathway via phosphorylation of LATS1/2 and subsequent reduction in YAP levels and YAP/TAZ target genes. These results identify BMI1 as a potential therapeutic vulnerability in ARMS and warrant further investigation of BMI1 in ARMS and other sarcomas. [ABSTRACT FROM AUTHOR]

Lung cancer (LC) is the leading cause of cancer‐related mortality. However, the molecular mechanisms associated with the development of metastasis are poorly understood. Understanding the biology of LC metastasis is critical to unveil the molecular mechanisms for designing targeted therapies. We developed two genetically engineered LC mouse models KrasG12D/+; Trp53R172H/+; Ad‐Cre (KPA) and KrasG12D/+; Ad‐Cre (KA). Survival analysis showed significantly (P = 0.0049) shorter survival in KPA tumor‐bearing mice as compared to KA, suggesting the aggressiveness of the model. Our transcriptomic data showed high expression of N‐acetylgalactosaminide alpha‐2, 6‐sialyltransferase 1 (St6galnac‐I) in KPA compared to KA tumors. ST6GalNAc‐I is an O‐glycosyltransferase, which catalyzes the addition of sialic acid to the initiating GalNAc residues forming sialyl Tn (STn) on glycoproteins, such as mucins. Ectopic expression of species‐specific p53 mutants in the syngeneic mouse and human LC cells led to increased cell migration and high expression of ST6GalNAc‐I, STn, and MUC5AC. Immunoprecipitation of MUC5AC in the ectopically expressing p53R175H cells exhibited higher affinity toward STn. In addition, ST6GalNAc‐I knockout (KO) cells also showed decreased migration, possibly due to reduced glycosylation of MUC5AC as observed by low STn on the glycoprotein. Interestingly, ST6GalNAc‐I KO cells injected mice developed less liver metastasis (P = 0.01) compared to controls, while colocalization of MUC5AC and STn was observed in the liver metastatic tissues of control mice. Collectively, our findings support the hypothesis that mutant p53R175H mediates ST6GalNAc‐I expression, leading to the sialyation of MUC5AC, and thus contribute to LC liver metastasis. [ABSTRACT FROM AUTHOR]

Purpose/objectives: To investigate dental students' perceptions and concerns regarding the COVID‐19 pandemic, their coping strategies and support resources, and their perceived stress levels. Methods: A customized 19‐item survey and the perceived stress scale (PSS) were applied to undergraduate dental students from the US, Spain, Ireland, Chile, India, and Brazil between April 10 and July 5, 2020. Linear modeling and mediation analysis were used to explore the relationships among demographics, stressors, coping mechanisms, social support, and stress Results: A total of 4475 students responded to the survey. The majority (72.4%) were women, and 52.3% had no COVID‐19 training at the time of the survey. The students reported that they had to accommodate to changes in patient care (96.6%) and didactic learning (95.2%) activities, while 88.5% of the respondents indicated at least one of their courses moved online. Transition to online courses went "smoothly with some troubles" for 51.8% of the respondents, and 48.3% perceived the faculty as prepared for the online transition; however, 45.9% reported feeling extremely concerned about the impact of COVID‐19 on their education. The average PSS score was 21.9 of 40 (moderate stress). Multivariate models were built for participants with full data (n = 3899). Being male, having completed more dental coursework, and perceiving a smoother transition were associated with lower PSS scores; more concern about academic progress was associated with higher PSS. Faculty support mediated the relationship between a smoothness of transition and concern about academic progress and PSS scores Conclusion: Stress caused by the pandemic may be alleviated by smoother transition and good faculty support. [ABSTRACT FROM AUTHOR]

Purpose/objectives: Silver diamine fluoride (SDF) is a promising therapy for arresting and preventing caries in difficult to treat, high-risk populations, including institutionalized older adults. This study investigates the knowledge and perceptions about SDF of graduating dental students in multiple U.S. dental schools, as well as their willingness to use SDF in their practices.Methods: A survey was designed consisting of 21 total questions: 6 questions regarding students' demographic information and their SDF content exposure contextualization, 8 questions tailored to investigate 2 domains regarding students' knowledge about SDF (properties and indication), and 7 questions aiming to investigate 3 domains regarding students' perceptions about SDF (SDF usefulness, appropriateness of using SDF, and willingness to use SDF when in private practice). The survey was then distributed to graduating dental students at 7 U.S. dental schools.Results: A total of 386 surveys (response rate of 55%) was collected from 7 schools in the Spring of 2019. The median score resulting from the SDF content exposure questions was 3 (SD = 1.43) from a range of 0-5. The median score from knowledge about SDF properties was 4 (SD = 1.18) from a range of 0 to 6. In the multivariate analysis, a linear model found that the covariates "SDF Usefulness", "SDF Appropriateness" and "SDF Patient Willingness to Use" were significantly associated with higher student willingness to use SDF (R2  = 0.395).Conclusion: The results indicated that the graduating students have a positive perception of SDF regarding its usefulness and appropriateness. Graduating students appear inclined to utilize SDF upon entering private practice. [ABSTRACT FROM AUTHOR]

Agricultural intensification is a leading threat to bird conservation. Highly diversified farming systems that integrate livestock and crop production might promote a diversity of habitats useful to native birds foraging across otherwise‐simplified landscapes. At the same time, these features might be attractive to nonnative birds linked to a broad range of disservices to both crop and livestock production. We evaluated the influence of crop–livestock integration on wild bird richness and density along a north‐south transect spanning the U.S. West Coast. We surveyed birds on 52 farms that grew primarily mixed vegetables and fruits alone or integrated livestock into production. Crop–livestock systems harbored higher native bird density and richness relative to crop‐only farms, a benefit more pronounced on farms embedded in nonnatural landscapes. Crop–livestock systems bolstered native insectivores linked to the suppression of agricultural pest insects but did not bolster native granivores that may be more likely to damage crops. Crop–livestock systems also significantly increased the density of nonnative birds, primarily European Starlings (Sturnus vulgaris) and House Sparrows (Passer domesticus) that may compete with native birds for resources. Models supported a small, positive correlation between nonnative density and overall native bird density as well as between nonnative density and native granivore density. Relative to crop‐only farms, on average, crop–livestock systems exhibited 1.5 times higher patch richness, 2.4 times higher density of farm structures, 7.3 times smaller field sizes, 2.4 times greater integration of woody crops, and 5.3 times greater integration of pasture/hay habitat on farm. Wild birds may have responded to this habitat diversity and/or associated food resources. Individual farm factors had significantly lower predictive power than farming system alone (change in C statistic information criterion (ΔCIC) = 80.2), suggesting crop–livestock systems may impact wild birds through a suite of factors that change with system conversion. Collectively, our findings suggest that farms that integrate livestock and crop production can attract robust native bird communities, especially within landscapes devoted to intensified food production. However, additional work is needed to demonstrate persistent farm bird communities through time, ecophysiological benefits to birds foraging on these farms, and net effects of both native and nonnative wild birds in agroecosystems. [ABSTRACT FROM AUTHOR]

Summary: Background: Dynamic laryngeal collapse (DLC) associated with poll flexion is the most common disorder of the upper respiratory tract (URT) in the Norwegian‐Swedish Coldblooded Trotter (NSCT). The disorder, which has also been diagnosed in other breeds of trotters and gaited horses, appears to be related to anatomic phenotypes and only occurs during poll flexion when the horse is exercised 'on the bit'. Objectives: Identify genomic regions associated with DLC in the NSCT by combining a rigorous phenotyping protocol with genomic data from a high‐density equine genotyping array. Study design: Prospective case/control study. Methods: High‐speed treadmill endoscopy was used to phenotype horses (n = 61) for DLC, distinguishing between cases and controls. Genome‐wide association (GWA) analysis of DLC status was then performed using a principal component approach (PCA) with haplotype analyses subsequently performed for regions containing single‐nucleotide polymorphisms (SNPs) above the suggestive genome‐wide significance (GWS) threshold (P<1.0 × 10−5). Results: One region containing 10 SNPs (Equus caballus chromosome [ECA] 7: 89,601,935–94,647,192) was above the suggestive GWS threshold. Two inferred haplotypes in this region demonstrated significant differences (P<0.001) between cases and controls, with the most frequent haplotype resulting in a significantly increased risk of DLC. Main limitations: Small sample size as a result of stringent phenotyping protocols. Conclusions: The current study highlights a candidate genomic region on ECA7 as potentially important with regard to the manifestation of DLC. Further exploration of this region and the genes included within it will bring veterinarians and researchers closer to fully understanding the biological mechanisms underlying DLC in horses. [ABSTRACT FROM AUTHOR]

Programs to control predatory fishes have successfully increased the survival of imperiled prey fishes in some cases, but efficacy depends on the population dynamics and ecological interactions between the predators, prey, and the rest of the community. In California's Sacramento–San Joaquin Delta, extremely low survival of downstream‐migrating juvenile salmonids has been ascribed in part to predation by abundant nonnative fishes that have become naturalized in the system. This study aimed to determine the effectiveness of removing some of these predatory fish to increase the survival of juvenile salmonids in the San Joaquin River. In 2014 and 2015, we used predation event recorders (PERs) to estimate predation rates before and after localized, experimental predatory fish removals and additions. We also estimated survival of juvenile Chinook Salmon Oncorhynchus tshawytscha before and after predator manipulations in 2014 using acoustic tags. We found no statistically significant evidence for an effect of predator removals or additions on survival rates of Chinook Salmon (as measured by acoustic tags) or on predation rates (as measured by PERs), despite a one‐time reduction of approximately 40–70% of all predators from "removal" sites and relocation to "addition" sites. We review examples of successful predator control programs elsewhere, highlighting key differences between those programs and our study in the Sacramento–San Joaquin Delta. [ABSTRACT FROM AUTHOR]

Background: The retina is increasingly investigated as a site of Alzheimer's Disease (AD) manifestation, with amyloid β (Aβ) plaques being found in the retina of AD and mild cognitively impaired (MCI) patients, however functional changes in the retina are poorly understood. Retinal imaging techniques can be utilized for neurodegenerative biomarkers, since the retina is the only CNS organ available for noninvasive tracking. Method: Retinal imaging techniques were using to compare the retina of wild‐type (WT) or AD transgenic mice containing the APP: Swedish and Indiana mutation expressed under the prion promoter (TgCRND8) at 20 and 30 weeks of age (n = 10 mice per genotype). Optical coherence tomography (OCT) for retinal thickness assessment, Fundus Autofluorescence Imaging (FAF) for aggregate visualization, and electroretinogram (ERG) for retinal neuronal electrical activity were performed. Aβ ELISA was also performed on retina and cortex to evaluate amyloid burden correlation. Result: Mean retinal insoluble Aβ 1‐42 was significantly elevated in TgCRND8 mice relative to WT at 23 weeks (35 vs 4.2 pg/mL, P<0.05) and 42 weeks (77.9 vs 7.5 pg/mL, P<0.05), and correlated with insoluble cortical brain Aβ 1‐42 (Pearson's r = 0.847, P = 0.001). Total retinal thickness was significantly higher in TgCRND8 mice relative to WT (233 µm vs 225 µm, P<0.01). Specifically, the retinal nerve fiber layer (RNFL) was thicker in TgCRND8 mice (28.5 vs 22.9 µm, P<0.01). FAF imaging revealed significant autofluorescent aggregate detection in TgCRND8 mice both in the RNFL (94.3 vs 59.8 spots, P<0.01) and outer plexiform layer (OPL) (87.8 vs 49.3 spots, P<0.01). Lastly ERG revealed reduced b wave amplitude in TgCRND8 mice relative to WT (197.45 vs 457.9 µV, P<0.001) and photopic negative response (PhNR) wave amplitude (8.2 µm vs 25.7 457.9 µV, P<0.001). These values are indicative of dysfunctional bipolar and retinal ganglion cell electrical activity. Conclusion: TgCRND8 mice have functional changes in retina thickness, FAF lipofuscin deposition, and ERG, which may prove invaluable as biomarkers for pre‐clinical AD diagnosis, monitoring and drug efficacy trials. [ABSTRACT FROM AUTHOR]

Twin-row planting of soybean [Glycine max (L.) Merr.] has become a popular practice in Mississippi and in much of the midsouthern United States. With the use of twin-row planters, there is the potential for a number of planter errors. However, there is little information on the effect of twin-row planter errors on soybean growth, seed yield, and replant decisions. Field experiments were conducted in 2016 and 2017 in Mississippi to evaluate the effects of planter errors for four soybean varieties of different relative maturity groups (4.2, 4.7, 4.9, and 5.4) on soybean seed yield, canopy closure, and replant decisions for two soil textures (clay and sandy loam) commonly used for soybean production in Mississippi. Four planter errors associated with the twin-row planting system included a control (None) consisting of the full intended stand with two normal twin rows within a bed, one single row from a twin-row pair missing (Single), one row of a twin row pair missing in two adjacent rows (Separate), and both twin rows within a row missing (Whole). Whole planter error reduced soybean yield compared with None by 11 and 12% on clay and sandy loam soils, respectively. However, Single and Separate planter error did not reduce yield compared with None. Results indicate that it would not be economically beneficial to replant a soybean crop for any planter error at current soybean prices. [ABSTRACT FROM AUTHOR]

Plant micro RNAs are small RNAs that are important for genetic regulation of processes such as plant development or environmental responses. Specific micro RNAs accumulate in the phloem during phosphate starvation, and may act as long-distance signalling molecules., We performed quantitative PCR on Arabidopsis hypocotyl micrograft tissues of wild-type and hen1-6 mutants to assess the mobility of several phosphate starvation-responsive micro RNA species., In addition to the previously confirmed mobile species miR399d, the corresponding micro RNA* (miR399d*) was identified for the first time as mobile between shoots and roots. Translocation by phosphate-responsive micro RNAs miR827 and miR2111a between shoots and roots during phosphate starvation was evident, while their respective micro RNA*s were not mobile., The results suggest that long-distance mobility of micro RNA species is selective and can occur without the corresponding duplex strand. Movement of miR399d* and root-localised accumulation of miR2111a* opens the potential for persisting micro RNA*s to be mobile and functional in novel pathways during phosphate starvation responses. [ABSTRACT FROM AUTHOR]

Four experiments tested the forgetting fixation hypothesis of incubation effects, comparing continuous vs. alternating generation of exemplars from three different types of categories. In two experiments, participants who listed as many members as possible from two different categories produced more responses, and more novel responses, when they alternated back and forth between the two categories, as compared to continuous uninterrupted listing from each of the two categories. This incubation effect was not found in Experiment 1, when participants were given taxonomic categories ( birds and clothing) for the generation task, but was found in Experiment 2 with sense impression categories ( cold things and heavy things), and in Experiment 3 with ad hoc categories ( equipment you take camping and fattening foods). A similar incubation effect was observed in Experiment 4 when a non-verbal task was given between category generation tasks, but only for flexibly defined categories. The results suggest that forgetting from one alternating listing period to the next in the form of altering category cue representations was consistent with the observed incubation effects. These alternating incubation effects have implications for understanding cognitive processes that underlie creative cognition. [ABSTRACT FROM AUTHOR]

Introduction: In 2009, the Australasian Society of Infectious Diseases published guidelines on the post-arrival health assessment of recently arrived refugees. Since then, the number of refugees and asylum seekers reaching Australia has increased substantially (17 555 refugees in 2015-16) and the countries of origin have changed. These groups are likely to have had poor access to health care pre-arrival and, consequently, are at risk of a range of chronic and infectious diseases. We established an advisory group that included infectious diseases physicians, general practitioners, public health specialists, paediatricians and refugee health nurses to update the 2009 guidelines.Main recommendations: All people from refugee-like backgrounds, including children, should be offered a tailored comprehensive health assessment and management plan, ideally within 1 month of arrival in Australia. This can be offered at any time if initial contact with a GP or clinic is delayed. Recommended screening depends on history, examination and previous investigations, and is tailored based on age, gender, countries of origin and transit and risk profile. The full version of the guidelines is available at http://www.asid.net.au/documents/item/1225.Changes in management as a result of this guideline: These guidelines apply to all people from refugee-like backgrounds, including asylum seekers. They provide more information about non-communicable diseases and consider Asia and the Middle East as regions of origin as well as Africa. Key changes include an emphasis on person-centred care; risk-based rather than universal screening for hepatitis C virus, malaria, schistosomiasis and sexually transmissible infections; updated immunisation guidelines; and new recommendations for other problems, such as nutritional deficiencies, women's health and mental health. [ABSTRACT FROM AUTHOR]

Background: Substantial numbers of U.S. military veterans who served in recent conflicts experience mild traumatic brain injury. Data suggest that as many as 25% of veterans do not have a comprehensive traumatic brain injury evaluation to determine a diagnosis and develop a plan to treat symptoms. Technologies like clinical video telehealth offer a potential means to overcome travel distance and other barriers that can impact veteran receipt of a comprehensive traumatic brain injury evaluation after a positive screening; however, little is known about implementing clinical video telehealth in this context.Objective: To examine the perspectives of Veterans Health Administration health care providers on implementing clinical video telehealth technology for the assessment and treatment of mild traumatic brain injury among veterans of Operation Enduring Freedom, Operation Iraqi Freedom, and Operation New Dawn.Design: Qualitative; semistructured interviews.Setting: Veterans Health Administration Polytrauma System of Care.Participants: Twenty-six providers who participated in a Veterans Health Administration Rehabilitation and Prosthetic Services Teleconsultation Pilot Project for administering comprehensive traumatic brain injury evaluations over clinical video telehealth.Methods: Semistructured interviews that used content-analytic techniques to assess provider experiences implementing clinical video telehealth for veterans with traumatic brain injury, including inhibiting factors and best practices to administer comprehensive traumatic brain injury evaluations.Results: The most commonly reported inhibiting factors to implementing clinical video telehealth for traumatic brain injury evaluation and treatment included scheduling, setting up the clinic, and conducting physical examinations over a virtual modality. To enhance clinical video telehealth implementation, participants described best practices including establishing solid communication and relationships with staff, building rapport with patients, and recognizing the unique needs of patients with traumatic brain injury.Conclusions: Implementing clinical video telehealth programs involves coordinating multiple steps with providers at different sites, highlighting the need for effective communication. Provider-patient communication also emerged as vital to successful clinical video telehealth implementation. These findings suggest that providers would benefit from efforts to build communication competencies.Level Of Evidence: Not applicable. [ABSTRACT FROM AUTHOR]

Background: Trust and mistrust of the healthcare system by Black and African American individuals is often cited as a primary barrier to willingness to access healthcare and low representation in clinical research. The United States Public Health Service Syphilis Study at Tuskegee is most often cited as the sole reason for this mistrust, however similar and widespread injustices, as well as the history of segregated health systems particularly in the stroke belt, laid the foundation for current health and research inequities. Medical discourses and practices positioned the Black or African American body as "inferior" and created disparities in research, diagnostic practices, and service provision. Method: Our team collaborated with a historian of psychiatry and disability to document the historical factors that have created structural inequities in the South and beyond. The historian (KS) collected archival material related to Milledgeville Central State Hospital in Georgia including physical documents like annual reports, case studies, policies and procedures, and letters from patients. Annual reports which contained extensive diagnostic data were photocopied, computerized and analyzed to characterize patient diagnosis (mental, physical, age‐related), cause of death, and cost expended per patient, by race and gender. Results: Our data documents the impact of segregated services, racist medical attitudes, abusive treatments, malpractices, physical labor exploitation, unlivable conditions, use of unapproved drugs, and involuntarily sterilizations in B/AA patients from 1956‐1970. Significant diagnostic disparities by race were obvious especially for "cerebral arteriosclerosis" and "schizophrenia". Here we present data on first admissions diagnosis by age, gender, and race, as well as death rate and cause, and group level fiscal reports. Conclusion: An analysis of historical data demonstrates clear linkages with current issues and practices. Addressing structural racism in research, education and service provision requires an honest reckoning with past practices that persist today. We demonstrate the ways that scientific teams can work with trained historians to unpack the consequences of past policies and set out a method and agenda for collaborative interdisciplinary research moving forward. [ABSTRACT FROM AUTHOR]

Background: GRN mutations cause frontotemporal dementia (FTD) due to haploinsufficiency of progranulin. Several microRNAs (miRs), including miR‐29b, have been reported to negatively regulate progranulin protein levels. Here, we tested if antisense oligonucleotides (ASOs) – which are versatile modulators of target mRNA/protein levels – can be used to increase progranulin levels by sterically blocking the miR‐29b binding site. Method: We designed 48 ASOs targeting the miR‐29b binding site in the 3' UTR of the human GRN mRNA. We treated H4 neuroglioma cells and iPSC‐derived neurons with these ASOs and subsequently measured progranulin protein levels by western blot and ELISA. We performed further studies to determine the mechanism of action of these ASOs using ribosomal profiling, metabolic labeling, and FRET assays. Results: We identified 16 ASOs that increased progranulin protein levels in a dose‐dependent manner. Ribosomal profiling experiments revealed that cells treated with ASOs had marked enrichment in GRN mRNA in heavy polyribosome fractions, compared to cells treated with a scrambled control ASO, suggesting that the ASOs increase the rate of progranulin translation. Consistent with this, ASO treatment resulted in increased levels of newly synthesized progranulin protein. FRET‐based assays showed that ASOs can effectively compete miR‐29b from its binding site in the GRN 3' UTR RNA under in vitro conditions. Conclusions: Together, our results demonstrate that ASOs can be used to effectively increase target protein levels by partially blocking miR binding sites. This strategy may be therapeutically feasible for progranulin‐deficient FTD as well as other conditions of haploinsufficiency. [ABSTRACT FROM AUTHOR]

The aim of this study was to help inform faculty and curriculum leaders in academic dental institutions about the knowledge, skills, perceptions, and behavior of an institutionally diverse population of dental students with respect to evidence-based practice (EBP). A survey utilizing the validated Knowledge, Attitudes, Access, and Confidence Evaluation instrument developed by Hendricson et al. was conducted in 2012 with fourth-year dental students at seven geographically dispersed U.S. dental schools. The survey addressed elements of EBP knowledge, attitudes toward EBP, behavior in accessing evidence, and perceptions of competence in statistical analysis. A total of 138 students from the seven schools participated. A slight majority of these students correctly responded to the knowledge of critical appraisal questions. While the students demonstrated positive attitudes about EBP, they did not report high levels of confidence in their critical appraisal skills. The findings also showed that the students accessed various sources of evidence with differing frequencies. The most frequently accessed resources were colleagues, the Internet (excluding Cochrane Database of Systematic Reviews), and textbooks. The results of this study help to identify areas for improvement in EBP education in order to advance dental students' preparation to become evidence-based practitioners. [ABSTRACT FROM AUTHOR]

Aberrant Wnt signaling frequently occurs in pancreatic cancer (PC) and contributes to disease progression/metastases. Likewise, the transmembrane-mucin MUC4 is expressed de novo in early pancreatic intraepithelial neoplasia (PanINs) and incrementally increases with PC progression, contributing to metastasis. To determine the mechanism of MUC4 upregulation in PC, we examined factors deregulated in early PC progression, such as Wnt/β-catenin signaling. MUC4 promoter analysis revealed the presence of three putative TCF/LEF-binding sites, leading us to hypothesize that MUC4 can be regulated by β-catenin. Immunohistochemical (IHC) analysis of rapid autopsy PC tissues showed a correlation between MUC4 and cytosolic/nuclear β-catenin expression. Knock down (KD) of β-catenin in CD18/HPAF and T3M4 cell lines resulted in decreased MUC4 transcript and protein. Three MUC4 promoter luciferase constructs, p3778, p3000, and p2700, were generated. The construct p3778, encompassing the entire MUC4 promoter, elicited increased luciferase activity in the presence of stabilized β-catenin. Mutation of the TCF/LEF site closest to the transcription start site (i.e., −2629/−2612) and furthest from the start site (i.e., −3425/−3408) reduced MUC4 promoter luciferase activity. Transfection with dominant negative TCF4 decreased MUC4 transcript and protein levels. Chromatin immunoprecipitation confirmed enrichment of β-catenin on −2629/−2612 and −3425/−3408 of the MUC4 promoter in CD18/HPAF. Functionally, CD18/HPAF and T3M4 β-catenin KD cells showed decreased migration and decreased Vimentin, N-cadherin, and pERK1/2 expression. Tumorigenicity studies in athymic nude mice showed CD18/HPAF β-catenin KD cells significantly reduced primary tumor sizes and metastases compared to scrambled control cells. We show for the first time that β-catenin directly governs MUC4 in PC. [ABSTRACT FROM AUTHOR]

Background: Access to health information is crucial to persons living with a spinal cord injury or disorder (SCI/D). Although previous research has provided insights on computer and Internet use among persons with SCI/D, as well as how and where persons with SCI/D gather health information, few studies have focused on U.S. veterans with SCI/D.Objective: To characterize health information seeking among veterans with SCI/D and to examine the association between technology use and the characteristics of veterans with SCI/D.Design: Cross-sectional.Setting: Veterans Health Administration (VHA).Participants: Sample of 290 veterans with SCI/D who utilize services at 2 VHA SCI/D Centers.Methods: Postal mail survey.Main Outcome Measurements: Extent of computer, Internet, and text messaging use, information source use, and e-Health literacy rates.Results: The survey response rate was 38%. The majority of respondents were male (97.2%), younger than 65 years (71.0%), and white (71.7%). Of the respondents, 64.8% indicated that they use a computer, 62.9% reported use of the Internet, and 26.2% reported use of text messaging. The mean overall e-Health Literacy Scale score was 27.3 (standard deviation = 7.2). Similar to findings reported in studies focused outside the veteran population, the most frequent source that veterans turned to for information about SCI/D was a health professional (85.1%); this was also the most frequent source that veterans indicated they would turn to first to get information about SCI/D (75.9%). Other frequently reported sources of information included other persons with SCI/D (41.0%), Internet resources (31.0%), and family and friends (27.9%).Conclusions: Fairly high levels of computer and Internet use exist among veterans with SCI/D. Veterans with SCI/D also have a strong preference for people-particularly health professionals, and to a lesser extent peers and family and friends-as sources of information about SCI/D. These findings highlight the importance of combining technology and human interaction to meet the information needs of this population. [ABSTRACT FROM AUTHOR]

This point/counterpoint article discusses the strengths and weaknesses of incorporating Massive Open Online Courses (MOOCs) into dental education, focusing on whether this relatively new educational modality could impact traditional dental curricula. Viewpoint 1 asserts that MOOCs can be useful in dental education because they offer an opportunity for students to learn through content and assessment that is delivered online. While specific research on MOOCs is limited, some evidence shows that online courses may produce similar learning outcomes to those in face-to-face courses. Given that MOOCs are intended to be open source, there could be opportunities for dental schools with faculty shortages and financial constraints to incorporate these courses into their curricula. In addition to saving money, dental schools could use MOOCs as revenue sources in areas such as continuing education. Viewpoint 2 argues that the hype over MOOCs is subsiding due in part to weaker than expected evidence about their value. Because direct contact between students, instructors, and patients is essential to the dental curriculum, MOOCs have yet to demonstrate their usefulness in replacing more than a subset of didactic courses. Additionally, learning professionalism, a key component of health professions education, is best supported by mentorship that provides significant interpersonal interaction. In spite of the potential of early MOOC ideology, MOOCs in their current form require either further development or altered expectations to significantly impact dental education. [ABSTRACT FROM AUTHOR]

Seven ripping treatments designed to improve soil physical conditions for revegetation were compared on a test pad simulating an earthen cover for a waste disposal cell. The field test was part of study of methods to convert compacted-soil waste covers into evapotranspiration covers. The test pad consisted of a compacted layer of fine-textured soil simulating a barrier protection layer overlain by a gravelly sand bedding layer and a cobble armor layer. Treatments included combinations of soil-ripping implements (conventional shank [CS], wing-tipped shank [WTS], and parabolic oscillating shank with wings [POS]), ripping depths, and number of passes. Dimensions, dry density, moisture content, and particle size distribution of disturbance zones were determined in two trenches excavated across rip rows. The goal was to create a root-zone dry density between 1.2 and 1.6 Mg m-3 and a seedbed soil texture ranging from clay loam to sandy loam with low rock content. All treatments created V-shaped disturbance zones as measured on trench faces. Disturbance zone size was most influenced by ripping depth. Winged implements created larger disturbance zones. All treatments lifted fines into the bedding layer, moved gravel and cobble down into the fine-textured protection layer, and thereby disrupted the capillary barrier at the interface. Changes in dry density within disturbance zones were comparable for the CS and WTS treatments but were highly variable among POS treatments. Water content increased in the bedding layer and decreased in the protection layer after ripping. The POS at 1.2-m depth and two passes created the largest zone with a low dry density (1.24 Mg m-3) and the most favorable seedbed soil texture (gravely silt loam). However, ripping also created large soil aggregates and voids in the protection layer that may produce preferential flow paths and reduce water storage capacity. [ABSTRACT FROM AUTHOR]

The aim of the present study was to retrospectively evaluate the clinical signs, MRI characteristics, interobserver agreement and outcome after medical treatment in dogs affected by cervical intervertebral foraminal disc extrusion (CIFDE). The medical records of three referral institutions were searched for dogs diagnosed with CIFDE between 2010 and 2012. Thirteen dogs were identified with CIFDE; affected dogs often had a normal neurological examination, with cervical hyperaesthesia and lameness as the most common clinical signs. On MRI, sagittal images showed no evidence of compression of the spinal cord; CIFDE could be identified only on transverse sections in all cases. An excellent interobserver agreement was found in the localisation of the affected intervertebral disc space, and a substantial agreement was found on the detection of CIFDE versus foraminal stenosis caused by overgrowing articular processes. All but two dogs recovered completely, and they were considered free of clinical signs without analgesia within a median of 7.5 weeks (range: 2-20) after medical management was started. The remaining two dogs were surgically treated followed by complete recovery. In view of our findings, the importance of a thorough MRI investigation in dogs presenting with cervical hyperaesthesia as the sole clinical sign should be highlighted. [ABSTRACT FROM AUTHOR]

Objective: To present currently known basic science and on-ice influences of sport related concussion (SRC) in hockey, building upon the Ice Hockey Summit I action plan (2011) to reduce SRC. Methods: The prior summit proceedings included an action plan intended to reduce SRC. As such, the proceedings from Summit I served as a point of departure, for the science and discussion held during Summit II (Mayo Clinic, Rochester MN, October, 2013). Summit II focused on Basic Science of Concussions in Ice Hockey: Taking Science Forward; (2) Acute and Chronic Concussion Care: Making a Difference; (3) Preventing Concussions via Behavior, Rules, Education and Measuring Effectiveness; (4) Updates in Equipment: their Relationship to Industry Standards and (5) Policies and Plans at State, National and Federal Levels to reduce SRC. Action strategies derived from the presentations and discussion described in these sectors were subsequently voted on for purposes of prioritization. The following proceedings include the knowledge and research shared by invited faculty, many of whom are health care providers and clinical investigators. Results: The Summit II evidence based action plan emphasizes the rapidly evolving scientific content of hockey SRC. It includes the most highly prioritized strategies voted on for implementation to decrease concussion. Conclusions: The highest priority action items identified from the Summit include: 1) eliminate head hits from all levels of ice hockey, 2) change body checking policies, and 3) eliminate fighting in all amateur and professional hockey. [ABSTRACT FROM AUTHOR]

Traumatic brain injury is an important cause of hypopituitarism in human beings, but limited information exists in the veterinary literature regarding this condition. The primary study objective was to investigate whether hypothalamic-anterior pituitary axis dysfunction exists following traumatic brain injury in 17 client owned dogs. In this retrospective, observational, open, cohort study, information about dogs presented to four separate referral centres between April 2008 and October 2013 was reviewed. Cases were included if they had suffered from non-fatal traumatic brain injury, resulting in neurological dysfunction, and followup evaluation included measurement of the serum concentration of insulin-like growth factor 1 (IGF-1), endogenous adrenocorticotrophic hormone (ACTH), basal cortisol, thyroidstimulating hormone, total thyroxine (TT4) and, if appropriate, free thyroxine. Decreased IGF-1 concentration was the most common abnormality detected (7/17, 41 per cent; median 132 ng/ml, range <15-536), followed by a decreased TT4 concentration (4/17, 23 per cent; median 19, range 4-49). Basal cortisol concentration was less than 20 nmol/l in two cases (2/17, 12 per cent; median 65, range <20-1735), with concurrently undetectable ACTH (<5 pg/ml). This study demonstrates that dogs with a history of traumatic brain injury can develop endocrine abnormalities indicative of hypothalamic-anterior pituitary dysfunction. [ABSTRACT FROM AUTHOR]

Meningoencephalitis of unknown origin (MUO) is a common inflammatory CNS disease in dogs, with a variable and unpredictable outcome. MRI and cerebrospinal fluid (CSF) features were prospectively evaluated to establish their utility as prognostic markers for predicting mortality, relapse and long-term outcome in 39 dogs with MUO. MRI and CSF analysis were performed at initial diagnosis and three months into treatment with prednisolone and cytosine arabinoside. When possible, MRI was repeated every 12 months thereafter. Median survival time was 26 days. All deaths occurred within 52 days of diagnosis (22/39; 56 per cent). One-third (13/39) died within 72 hours of diagnosis. Outcome was good or excellent in 12/17 surviving dogs. Loss of the cerebral sulci and foramen magnum herniation on MRI were associated with increased risk of mortality. An abnormal CSF analysis at the three-month re-examination was associated with increased risk of relapse (P=0.04). The combination of MRI and CSF analysis provided a greater sensitivity for predicting relapse than one modality alone. Discontinuing treatment before MRI lesions resolved always resulted in relapse. The presence of certain MRI characteristics may indicate an increased risk of mortality. Dogs alive three months following diagnosis have a very low risk of death due to MUO. [ABSTRACT FROM AUTHOR]

Objective: To describe the early results of the U.S. Department of Veterans Affairs (VA) screening program for traumatic brain injury (TBI) and to identify patient and facility characteristics associated with receiving a TBI screen and results of the screening. Design: National retrospective cohort study. Setting: VA Medical facilities. Patients: A total of 170,681 Operation Enduring Freedom and/or Operation Iraqi Freedom (OEF/OIF) Veterans who sought care at VA medical facilities from April 2007 to September 30, 2008. Methods: Data were abstracted from VA administrative and operational databases, including patient demographics, facility characteristics, and outcomes. Main Outcome Measurements: The main outcomes were receipt of and results of the TBI screen. Results: The majority of veterans eligible received the TBI screen (91.6%). Screening rates varied by patient and facility characteristics. In all, 25% of screened veterans had probable TBI exposure, in which the majority of the exposures were blasts (85.0%). The rate of a positive TBI screen was 20.5% for the screened cohort. Male gender, service in the army, multiple deployments, and mental health diagnoses in the previous year were associated with a positive screen. Conclusions: TBI screening rates are high in VA; concomitant mental health diagnoses were highly prevalent in individuals with positive TBI screens. These data indicate that there will be a significant need for long-term health care services for veterans with TBI symptomatology. [ABSTRACT FROM AUTHOR]

Current biofuel feedstock crops such as corn lead to large environmental losses of N through nitrate leaching and N2O emissions; second‐generation cellulosic crops have the potential to reduce these N losses. We measured N losses and cycling in establishing miscanthus (Miscanthus × giganteus), switchgrass (Panicum virgatum L. fertilized with 56 kg N ha−1 yr−1), and mixed prairie, along with a corn (Zea mays L.)–corn–soybean [Glycine max (L.) Merr.] rotation (corn fertilized at 168–202 kg N ha−1). Nitrous oxide emissions, soil N mineralization, mid‐profile nitrate leaching, and tile flow and nitrate concentrations were measured. Perennial crops quickly reduced nitrate leaching at a 50‐cm soil depth as well as concentrations and loads from the tile systems (year 1 tile nitrate concentrations of 10–15 mg N L−1 declined significantly by year 4 in all perennial crops to <0.6 mg N L−1, with losses of <0.8 kg N ha−1 yr−1). Nitrous oxide emissions were 2.2 to 7.7 kg N ha−1 yr−1 in the corn–corn–soybean rotation but were <1.0 kg N ha−1 yr−1 by year 4 in the perennial crops. Overall N balances (atmospheric deposition + fertilization + soybean N2 fixation – harvest, leaching losses, and N2O emissions) were positive for corn and soybean (22 kg N ha−1 yr−1) as well as switchgrass (9.7 kg N ha−1 yr−1) but were −18 and −29 kg N ha−1 yr−1 for prairie and miscanthus, respectively. Our results demonstrate rapid tightening of the N cycle as perennial biofuel crops established on a rich Mollisol soil. [ABSTRACT FROM AUTHOR]

Abstract: This review is based on a case report that concerns a young female athlete who experienced some of the negative aspects of exercise. Overtraining, a negative byproduct of excessive exercise, can turn the positive psychosocial and physiologic benefits of regular physical activity into an activity detrimental to one''s health. With the proper psychological skills and appropriate exercise regimen, these negatives can be turned into positives. Once learned, the psychosocial benefits of exercise, as well as the positive implications, will become more prevalent, similar to the way in which proper physical training helps one become more fit over time. [Copyright &y& Elsevier]

Idiopathic facial neuropathy in dogs is conventionally diagnosed by exclusion of structural and metabolic causes of a single cranial neuropathy. To investigate the reliability and validity of MRI for supporting a diagnosis of facial neuropathy in dogs, we examined MR images of the brainstem from 20 dogs with presumed idiopathic facial neuropathy, and 20 control dogs. Two different types of MRI sequence were compared: volumetric interpolated breath-hold examination MRI (VIBE-MRI), and conventional T1-weighted MRI. Three independent observers, blinded to the case details, were presented with VIBE-MR images in a random order, and were required to indicate the presence of hyperintensity of the facial nerve following intravenous contrast administration. The vestibulocochlear nerve was also assessed, and the whole process was repeated for conventional post-contrast T1-weighted images. Following contrast administration, the sensitivity of VIBE-MRI for detecting facial nerve abnormalities ranged from 86 per cent to 96 per cent, and the specificity from 87 per cent to 92 per cent; conventional T1-weighted images yielded a sensitivity of only 39 per cent - 65 per cent, and a specificity of 94 per cent - 96 per cent. MRI was not a sensitive detector of vestibulocochlear nerve abnormalities in 14 dogs with concurrent vestibular dysfunction. This study shows that VIBE-MRI is a robust technique for confirming presumed idiopathic facial neuropathy in dogs, and suggests an inflammatory component to this condition in a high proportion of dogs. [ABSTRACT FROM AUTHOR]

Natural rates of straying are difficult to quantify over large spatial scales using direct observations, particularly for long-lived fish species characterized by delayed sexual maturity and long interspawning intervals. Using multilocus microsatellite genotypes and likelihood-based statistical methods, we quantified rates of immigration and emigration for six genetically differentiated (meanFST= 0.041) lake sturgeonAcipenser fulvescenspopulations in Lake Michigan based on adults (n= 437) captured in tributaries during the spawning season. Estimated rates of straying were high (mean = 0.105), asymmetrical, and highly variable across populations. We found no significant association between the total length (a surrogate measure of age) of individuals that strayed and those that did not. Linear distance between streams was more predictive of straying rates andFSTthan least-cost distances estimated based on lakescape features (bathymetry and lake current patterns). Historical rates of gene flow estimated using coalescent analysis indicated a fully parameterized model with variable evolutionarily effective population sizes (θ range, 0.684–0.989), and variable and nonsymmetrical migration rates best explained the genetic data. Comparatively high estimates of relative historical gene flow from several numerically depressed populations suggest that these populations were once larger contributors to basinwide gene flow than indicated by estimates of contemporary straying rates. High rates of interpopulation straying contrast with highFST, suggesting that straying rates are poor indicators of successful reproduction following dispersal. Received June 29, 2011; accepted May 7, 2012 [ABSTRACT FROM PUBLISHER]

Objective: To critically analyze the contemporary published research that pertains to the individual components of complete decongestive therapy (CDT), as well as CDT as a bundled intervention in the treatment of lymphedema. Data Sources: Publications were retrieved from 11 major medical indices for articles published from 2004-2010 by using search terms for lymphedema and management approaches. Literature archives of the authors and reference lists were examined through 2011. Study Selection: A research librarian assisted with initial literature searches by using search terms used in the Best Practice for the Management of Lymphoedema, plus expanded terms, for literature related to lymphedema. Authors sorted relevant literature for inclusion and exclusion; included articles were sorted into topical areas for data extraction and assessment of level of evidence by using a published grading system and consensus process. The authors reviewed 99 articles, of which 26 met inclusion criteria for individual studies and 1 case study did not meet strict inclusion criteria. In addition, 14 review articles and 2 consensus articles were reviewed. Data Extraction: Information on study design and/or objectives, participants, outcomes, intervention, results, and study strengths and weaknesses was extracted from each article. Study evidence was categorized according to the Oncology Nursing Society Putting Evidence into Practice level of evidence guidelines after achieving consensus among authors. Data Synthesis: Levels of evidence were only moderately strong, because there were few randomized controlled trials with control groups, well-controlled interventions, and precise measurements of volume, mobility and/or function, and quality of life. Treatment interventions were often bundled, which makes it difficult to determine the contribution of each individual component of treatment to the outcomes achieved. Conclusions: CDT is seen to be effective in reducing lymphedema. This review focuses on original research about CDT as a bundled intervention and 2 individual components, manual lymph drainage and compression bandages. Additional studies are needed to determine the value and efficacy of the other individual components of CDT. [Copyright &y& Elsevier]

The article presents a study on actions made in a citizen action program that assure data quality and show the validity of citizen-generated data. The study involved data collected by secondary school students, as part of a program that quantitatively sampled benthic rocky intertidal communities in Maui, Molokai and Oahu in Hawaii from 2004 to 2007. The results showed that nonexpert students accurately collect community-level data in the range of the variation that occurs between researchers.

The subfornical organ (SFO), a sensory circumventricular organ lacking the normal blood-brain barrier with well documented roles in cardiovascular regulation, has recently been identified as a potential site at which the adipokine, leptin, may act to influence central autonomic pathways. Systemic and central leptin administration has been shown to increase blood pressure and it has been suggested that selective leptin resistance contributes to obesity-related hypertension. Given the relationship between obesity and hypertension, the present study aimed to investigate the cardiovascular consequences of the direct administration of leptin into the SFO of young lean rats and in the diet-induced obesity (DIO) rat model, which has been shown to be leptin-resistant. Leptin administration (500 fmol) directly into the SFO of young rats resulted in rapid decreases in blood pressure (BP) [mean area under the curve (AUC) = −677.8 ± 167.1 mmHg*s; n = 9], without an effect on heart rate (mean AUC = −21.2 ± 13.4 beats; n = 9), and these effects were found to be dose-related as microinjection of 5 pmol of leptin into the SFO had a larger effect on BP (mean AUC = −972.3 ± 280.1 mmHg*s; n = 4). These BP effects were also shown to be site-specific as microinjection of leptin into non-SFO regions or into the ventricle was without effect on BP (non-SFO: mean AUC = −22.4 ± 55.3 mmHg*s; n = 4; ventricle: mean AUC = 194.0 ± 173.0 mmHg*s; n = 6). By contrast, microinjection of leptin into leptin-resistant DIO rats was without effect on BP (mean AUC = 205.2 ± 75.1 mmHg*s; n = 4). These observations suggest that the SFO may be an important relay centre through which leptin, in normal weight, leptin responsive rats, acts to maintain BP within normal physiological limits through descending autonomic pathways involved in cardiovascular control and that, in obese, leptin-resistant, rats leptin no longer influences SFO neurones, resulting in an elevated BP, thus contributing to obesity-related hypertension. [ABSTRACT FROM AUTHOR]

The article discusses a qualitative study of community-based asylum seekers' access to primary health care services (PHCS) in New South Wales. The authors conducted semi-structured interviews with 12 asylum seekers, three nurses, one general practitioner and one manager about the impact of the difficulty in gaining access to PHCS on the AS' physical and mental health. They found that lack of access to PHCS resulted to physical suffering, anxiety and risk of deterioration in health status.

Objective: The objective of this proceeding is to integrate the concussion in sport literature and sport science research on safety in ice hockey to develop an action plan to reduce the risk, incidence, severity, and consequences of concussion in ice hockey. Methods: A rationale paper outlining a collaborative action plan to address concussions in hockey was posted for review 2 months before the Ice Hockey Summit: Action on Concussion. Focused presentations devoted specifically to concussion in ice hockey were presented during the Summit, and breakout sessions were used to develop strategies to reduce concussion in the sport. This proceedings and a detailed scientific review (a matrix of solutions) were written to disseminate the evidence-based information and resulting concussion reduction strategies. The manuscripts were reviewed by the authors, advisers, and contributors to ensure that the opinions and recommendations reflect the current level of knowledge on concussion in hockey. Results: Six components of a potential solution were articulated in the Rationale paper and became the topics for breakout groups that followed the professional, scientific lectures. Topics that formed the core of the action plan were metrics and databases; recognizing, managing, and returning to play; hockey equipment and ice arenas; prevention and education; rules and regulations; and expedient communication of the outcomes. The attendees in breakout sessions identified action items for each section. The most highly ranked action items were brought to a vote in the open assembly by using an Audience Response System. The strategic planning process was conducted to assess the following: Where are we at? Where must we get to? What strategies are necessary to make progress on the prioritized action items? Conclusions: Three prioritized action items for each component of the solution and the percentage of the votes received are listed in the body of this proceeding. [Copyright &y& Elsevier]

Earthquakes, lightning, and history-changing ideas are classic examples of powerful, unpredictable forces of nature. These sorts of phenomena have been difficult to explain and predict, an often frustrating fact as humans try to understand and control the significant influences in our lives. Historically, such phenomena have been attributed to supernatural sources, such as the Muses or various deities. And yet, as scientific inquiry continues to focus on such problems, we begin to make progress, albeit incremental, in comprehending such unpredictable occurrences. Earthquakes and lightning serve as analogies for the phenomenon referred to as insight. As such, these analogies may help us better understand where ideas come from; in each case, the rare, and potentially powerful phenomenon occurs unexpectedly, yet, as we learn more, it becomes better understood. Lightning is an electrical phenomenon, caused by particles colliding, and building up large electrical fields in the clouds. Earthquakes occur at the edges of tectonic plates, as the Earth releases its heat. Thus, we may begin to understand what causes insight, even though we cannot as yet predict specific occurrences of new insights. Analogies may not provide fully developed solutions to problems, but they can help us restructure our thinking about certain problems. Problem restructuring is especially appropriate when fixation obstructs problem solving. In this paper, we will explain a few of the fundamentals about human insight experiences, particularly in relation to what have been termed incubation effects. Putting a fixated problem aside can be helpful, and new ideas encountered during fixation can sometimes trigger new insights, particularly if useful analogies help resolve fixation. How does one overcome the counterproductive effects of fixation? We suggest a three-pronged approach to this problem: 1. Forgetting fixation, 2. Redefining problems, and 3. Using clues or hints to provoke new ideas. We will explain each of these ideas, in brief, after providing a background for insight, incubation, and fixation. [ABSTRACT FROM AUTHOR]