1. Cryo-Immunoelectron Microscopy of Adherent Cells Improved by the Use of Electrospun Cell Culture Substrates.
- Author
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Schmiedinger, Thomas, Vogel, Georg F., Eiter, Oliver, Pfaller, Kristian, Kaufmann, Walter A., Flörl, Angelika, Gutleben, Karin, Schönherr, Sabine, Witting, Barbara, Lechleitner, Thomas W., Ebner, Hannes‐L., Seppi, Thomas, and Hess, Michael W.
- Subjects
IMMUNOELECTRON microscopy ,CELL culture ,BIOCHEMISTRY ,IMMUNOGOLD labeling ,CYTOCHEMISTRY ,FLUORESCENCE microscopy - Abstract
Electrospun nanofibres are an excellent cell culture substrate, enabling the fast and non-disruptive harvest and transfer of adherent cells for microscopical and biochemical analyses. Metabolic activity and cellular structures are maintained during the only half a minute-long harvest and transfer process. We show here that such samples can be optimally processed by means of cryofixation combined either with freeze-substitution, sample rehydration and cryosection-immunolabelling or with freeze-fracture replica-immunolabelling. Moreover, electrospun fibre substrates are equally suitable for complementary approaches, such as biochemistry, fluorescence microscopy and cytochemistry. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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