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Summary: Bacteria adherence to the surface and the resulting biofilm have serious harmful effects in food. Biofilm formation in foods can lead to food deterioration and disease transfer easily. Some alternative applications are being tried to remove biofilms. Among these, biosurfactants and bacteriophages are promising owing to their low toxicity, easy availability, and high activity. In our study, the action of phage and rhamnolipid, a biosurfactant, on the biofilm of Salmonella Infantis, which is an important pathogen in food, was examined. Additionally, the efficacy of phages in the biocontrol of foods was tested. It is observed that the phage significantly reduces the bacterial concentration in milk in the first 4 h at 4 °C and 25 °C and maintains its effect for 24 h at 4 °C. The p_S3 phage, rhamnolipid (RML), and p_S3 phage + RML mixture on the 48‐h biofilm decreased the biofilm mass by 44.9–81.8%, 20.8–81.1%, and 12–60%, respectively. On a 1‐week‐old biofilm, the p_S3 phage, RML, and p_S3 phage + RML mixture decreased the biofilm mass by 43–60%, 55–72%, and 49.7–61.7%, respectively. It is seen that the p_S3 phage and RML co‐administration on biofilm can be a biosafe application in food production facilities where it is a more effective application in removing the biofilm in long‐term exposure. [ABSTRACT FROM AUTHOR]

This manuscript describes a previously unreported clinical case of Salmonella enterica serovar infantis associated with ulcerative keratitis in a dog. Local immunosuppression of the corneal surface likely contributed to this opportunistic infection. Antimicrobial therapy with topical ofloxacin, tobramycin, and systemic enrofloxacin was successful in resolving the infection. [ABSTRACT FROM AUTHOR]

The purpose of this study was to investigate bacterial load in ready-to-sale sheep meat with special reference to Salmonella. Samples were collected from 100 sheep carcasses from retail meat shops in domestic markets. On carcasses, where bacterial counts were obtained, the mean of the log10 aerobic plate count was 7.26 cfu g−1, and that of total coliform count and total Escherichia coli count was 4.11 log10 cfu g−1 and 3.03 log10 cfu g−1, respectively. All the samples (100) were found positive for coliforms, 49.0% were positive for E. coli and 3.0% were positive for Salmonella. The isolates were serotyped as Salmonella infantis having antigenic structure 6, 7: r: 1, 5. Antibiogram revealed highest (100.0%) sensitivity towards amikacin, ceftriaxone, ciprofloxacin, chloramphenicol, colistin sulphate, gentamicin and nalidixic acid followed by cefuroxime and tetracycline (66.67% each) and cotrimoxazole (33.33%). All the strains were resistant to ampicillin. [ABSTRACT FROM AUTHOR]