1. The presence of CD56/CD16 in T-cell acute lymphoblastic leukaemia correlates with the expression of cytotoxic molecules and is associated with worse response to treatment.
- Author
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Dalmazzo LF, Jácomo RH, Marinato AF, Figueiredo-Pontes LL, Cunha RL, Garcia AB, Rego EM, and Falcão RP
- Subjects
- Adolescent, Adult, Age Factors, Antigens, CD analysis, Antigens, CD34 analysis, Antigens, Differentiation, Myelomonocytic analysis, Biomarkers analysis, CD3 Complex analysis, Disease-Free Survival, Female, Flow Cytometry, Granzymes analysis, Humans, Immunophenotyping, Kaplan-Meier Estimate, Leukocyte Common Antigens analysis, Male, Perforin analysis, Platelet Count, Poly(A)-Binding Proteins analysis, Precursor T-Cell Lymphoblastic Leukemia-Lymphoma drug therapy, Precursor T-Cell Lymphoblastic Leukemia-Lymphoma mortality, Sialic Acid Binding Ig-like Lectin 3, Survival Rate, T-Cell Intracellular Antigen-1, Treatment Outcome, Young Adult, CD56 Antigen analysis, Killer Cells, Natural immunology, Precursor T-Cell Lymphoblastic Leukemia-Lymphoma immunology, Receptors, IgG analysis
- Abstract
Some cases of T-cell acute lymphoblastic leukaemia (ALL) express markers found in natural-killer (NK) cells, such as CD56 and CD16. Out of 84 T-cell ALL cases diagnosed at our Institution, CD56 and/or CD16 was detected in 24 (28.5%), which we designated T/NK-ALL group. Clinical features, laboratory characteristics, survival and expression of cytotoxic molecules were compared in T/NK-ALL and T-ALL patients. Significant differences were observed regarding age (24.9 vs. 16.4 years in T/NK-ALL and T-ALL, respectively, P = 0.006) and platelet counts (177 x 10(9)/l vs. 75 x 10(9)/l in T/NK-ALL and T-ALL, respectively, P = 0.03). Immunophenotypic analysis demonstrated that CD34, CD45RA and CD33 were more expressed in T/NK-ALL patients, whereas CD8 and terminal deoxynucleotidyl transferase were more expressed in T-ALL patients (P < 0.05). The mean overall survival (863 vs. 1869 d, P = 0.02) and disease-free survival (855 vs. 2095 d, P = 0.002) were shorter in patients expressing CD56/CD16. However, multivariate analysis identified CD56/CD16 as an independent prognostic factor only for DFS. Cytotoxic molecules were highly expressed in T/NK-ALL compared to T-ALL. Perforin, granzyme B and TIA-1 were detected in 12/17, 4/17 and 7/24 T/NK-ALL patients and in 1/20, 0/20 and 1/20 T-ALL respectively (P < 0.001, P = 0.036 and P = 0.054). Therefore, the presence of CD56/CD16 was associated with distinct clinical features and expression of cytotoxic molecules in the blasts.
- Published
- 2009
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