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SUMMARY Maize is one of the world's most important staple crops, yet its production is increasingly threatened by the rising frequency of high‐temperature stress (HTS). To investigate the genetic basis of anther thermotolerance under field conditions, we performed linkage and association analysis to identify HTS response quantitative trait loci (QTL) using three recombinant inbred line (RIL) populations and an association panel containing 375 diverse maize inbred lines. These analyses resulted in the identification of 16 co‐located large QTL intervals. Among the 37 candidate genes identified in these QTL intervals, five have rice or Arabidopsis homologs known to influence pollen and filament development. Notably, one of the candidate genes, ZmDUP707, has been subject to selection pressure during breeding. Its expression is suppressed by HTS, leading to pollen abortion and barren seeds. We also identified several additional candidate genes potentially underly QTL previously reported by other researchers. Taken together, our results provide a pool of valuable candidate genes that could be employed by future breeding programs aiming at enhancing maize HTS tolerance. [ABSTRACT FROM AUTHOR]

The continuous development of portable devices and new energy vehicles has led to an increasing demand for high‐performance lithium‐ion batteries (LIBs), and anode materials, as a key part of LIBs, are being explored. Germanium‐based anode materials are considered to have good potential for application because of their high theoretical capacity. In this work, a germanium phosphate glass precipitating GeO2 microcrystal was designed and used in LIBs as anode materials for the study. The presence of GeO2 crystals increases the specific surface area and also provides more reaction sites for lithiation reaction, resulting in the capacity of the glass anode. The discharge specific capacity of the germanium‐phosphorus glass anode was as high as 740.9 mAh g−1 after 600 cycles at a current density of 500 mA g−1. Interestingly, the synergistic effect between the glass matrix and the crystals not only increases the cycling capacity but also enhances the cycling stability of the structure. [ABSTRACT FROM AUTHOR]

Introduction: Although sirtuin 3 (SIRT3) is known to be involved in dexmedetomidine (DEX)‐mediated alleviation of renal ischemia and reperfusion injury, the influence of the association between DEX and SIRT3 on nephritis development remains unclear. In this study, the role of SIRT3 in DEX‐mediated amelioration of inflammation and oxidative stress in nephritis as well as the possible underlying mechanism were explored in vivo and in vitro. Methods: An animal model of glomerulonephritis was generated by injecting mice with interferon‐alpha (IFNα)‐expressing adenoviruses, and periodic acid–Schiff staining was then used to reveal pathogenicity‐related changes in the renal tissue. Additionally, human embryonic kidney cells (HEK293) and renal mesangial cells (RMCs) were treated with IFNα to establish cell models of inflammation in vitro. Results: DEX administration alleviated glomerulonephritis in the animal model and upregulated SIRT3 expression in the renal tissue. SIRT3 knockdown inhibited the renoprotective effects of DEX against nephritis. IFNα induced inflammation, oxidative stress, and apoptosis in the RMCs and HEK293 cells and reduced their growth, as evidenced by the evaluation of cytokine levels (enzyme‐linked immunosorbent assay), reactive oxygen species generation, catalase and superoxide dismutase activities, nuclear factor‐erythroid factor 2‐related factor 2/heme oxygenase‐1 signal transduction, apoptotic cell proportion, and cell viability. In addition to promoting SIRT3 expression, DEX inhibited IFNα‐induced inflammation, oxidative stress, and apoptosis in these cells and promoted their viability. SIRT3 knockdown partially reversed the beneficial effects of DEX on RMCs and HEK293 cells. Conclusions: Our results suggest that DEX exhibits renoprotective activity during nephritis progression, protecting renal cells against inflammatory injury by promoting SIRT3 expression. [ABSTRACT FROM AUTHOR]

Viticulture in China, like in many other major grape-growing countries around the world, is severely affected by downy mildew ( DM) disease caused by Plasmopara viticola (Pv). However, little has been known about the pathogenicity and genetic structure of the pathogen distributed in China. In this study, 206 single-sporangiophore Pv strains were isolated from Pv-infected leaves collected from the most important grape-growing regions in China. Among them, 29 strains, isolated from a majority of sampled regions and from the hosts with different DM resistance, were tested for their pathogenicity by inoculating into six grape cultivars with different levels of DM resistance. Significant difference in pathogenicity was observed among these strains. Seven pairs of SSR primers selected from previous reports were used for genetic studies of these 206 Pv strains. A total of 64 alleles and 193 genotypes were identified, suggesting that oosporic infection resulted from sexual reproduction made the major contributions to the disease epidemics during the growing season. Analysis of molecular variance ( AMOVA) and principal coordinate analysis ( PCoA) showed that most of genetic variations were within populations. In addition, low levels of pairwise FST value (ranged from 0.02 to 0.07) and high levels of gene flow were detected between populations. Results from this study suggest that long-distance dispersals of Pv oospore occur in China. [ABSTRACT FROM AUTHOR]

Summary: The oomycete pathogen Hyaloperonospora arabidopsidis delivers diverse effector proteins into host plant cells to suppress the plant's innate immunity. In this study, we investigate the mechanism of action of a conserved RxLR effector, HaRxLL470, in suppressing plant immunity.Genomic, molecular and biochemical analyses were performed to investigate the function of HaRxLL470 and the mechanism of the interaction between HaRxLL470 and the target host protein during H. arabidopsidis infection.We report that HaRxLL470 enhances plant susceptibility to H. arabidopsidis isolate Noco2 by interacting with the host photomorphogenesis regulator protein HY5. Our results demonstrate that HY5 is not only an important component in the regulation of light signalling, but also positively regulates host plant immunity against H. arabidopsidis by transcriptional activation of defense‐related genes. We show that the interaction between HaRxLL470 and HY5 compromises the function of HY5 as a transcription factor by attenuating its DNA‐binding activity.The present study demonstrates that HY5 positively regulates host plant defense against H. arabidopsidis whereas HaRxLL470, a conserved RxLR effector across oomycete pathogens, enhances pathogenicity by interacting with HY5 and suppressing transcriptional activation of defense‐related genes. [ABSTRACT FROM AUTHOR]