Search

This article explores how the notion of mobility resonates with visual responses to Sterne's fiction (1996-2016). With a specific focus on migration patterns of the Sterne/Tristram/Yorick triad, it is argued that artists have created, in a variety of media, a body of visual Sterneana characterised by embedded imaginary spaces and multiple interpretative frames through a fluctuating proximity with the text and the enduring ubiquity of eighteenth-century images in the form of graphic afterlives. Emblematic motifs and isolated fragments, as well as circulation in cyberspace, also raise the issue of how the word and image relationship is continuously reconfigured. [ABSTRACT FROM AUTHOR]

The Mre11-Rad50-Nbs1 ( MRN) complex is a central factor in the repair of DNA double-strand breaks ( DSBs). The ATP-dependent mechanisms of how MRN detects and endonucleolytically processes DNA ends for the repair by microhomology-mediated end-joining or further resection in homologous recombination are still unclear. Here, we report the crystal structures of the ATPγS-bound dimer of the Rad50 NBD (nucleotide-binding domain) from the thermophilic eukaryote Chaetomium thermophilum ( Ct) in complex with either DNA or CtMre11 RBD (Rad50-binding domain) along with small-angle X-ray scattering and cross-linking studies. The structure and DNA binding motifs were validated by DNA binding experiments in vitro and mutational analyses in Saccharomyces cerevisiae in vivo. Our analyses provide a structural framework for the architecture of the eukaryotic Mre11-Rad50 complex. They show that a Rad50 dimer binds approximately 18 base pairs of DNA along the dimer interface in an ATP-dependent fashion or bridges two DNA ends with a preference for 3′ overhangs. Finally, our results may provide a general framework for the interaction of ABC ATPase domains of the Rad50/ SMC/RecN protein family with DNA. [ABSTRACT FROM AUTHOR]