47 results on '"K. Yoshino"'
Search Results
2. Troponin-guided utilization of methylprednisolone pulse, intravenous immunoglobulin, and mycophenolate mofetil for successful control of immune checkpoint inhibitor-related myocarditis.
- Author
-
Toyoshima R, Uehara J, Matsuzaki Y, Yoshimura A, Kitano S, and Yoshino K
- Subjects
- Humans, Middle Aged, Drug Therapy, Combination methods, Immunosuppressive Agents administration & dosage, Immunosuppressive Agents adverse effects, Treatment Outcome, Immune Checkpoint Inhibitors adverse effects, Immune Checkpoint Inhibitors administration & dosage, Immunoglobulins, Intravenous administration & dosage, Methylprednisolone administration & dosage, Mycophenolic Acid administration & dosage, Myocarditis chemically induced, Myocarditis immunology, Myocarditis drug therapy, Myocarditis diagnosis
- Published
- 2024
- Full Text
- View/download PDF
3. Adverse events associated with postoperative outcomes of adjuvant anti-PD-1 antibody therapy in both acral and non-acral cutaneous melanomas: A multicenter, observational, post hoc analysis study.
- Author
-
Muto Y, Kambayashi Y, Kato H, Fukushima S, Ito T, Maekawa T, Fujisawa Y, Yoshino K, Uchi H, Matsushita S, Yamamoto Y, Amagai R, Ohuchi K, Hashimoto A, Asano Y, and Fujimura T
- Subjects
- Humans, Combined Modality Therapy, Lower Extremity, Retrospective Studies, Melanoma drug therapy, Melanoma surgery, Skin Neoplasms drug therapy, Skin Neoplasms surgery
- Abstract
Since anti-PD-1 Abs can cause irreversible immune-related adverse events (irAEs), the associations between their efficacies and the incidence of irAEs are important to evaluate the use of anti-PD-1Abs for the treatment of melanoma, especially in the adjuvant setting. The purpose of this post hoc analysis study was to retrospectively analyze the associations between recurrence-free survival (RFS) at 12 months and the onset of any irAEs in 31 non-acral cutaneous and 30 acral melanoma cases treated with anti-PD-1 Abs therapy at the adjuvant setting in Asians. There were 20 cases with greater than grade 1 AEs in both the acral and non-acral cutaneous groups. Of the acral melanoma, 10 cases were nails or toes, and 20 cases were soles and heels. The log-rank test showed that RFS was better in cases with AEs than in cases without AEs. The present study suggested that the different profiles of irAEs between non-acral cutaneous and acral melanoma might correlate with the different response to anti-PD1 Abs of melanoma in the adjuvant setting., (© 2023 Japanese Dermatological Association.)
- Published
- 2024
- Full Text
- View/download PDF
4. Treatment for taxane-resistant cutaneous angiosarcoma: A multicenter study of 50 Japanese cases.
- Author
-
Fujimura T, Maekawa T, Kato H, Ito T, Matsushita S, Yoshino K, Fujisawa Y, Ishizuki S, Segawa K, Yamamoto J, Hashimoto A, Kambayashi Y, and Asano Y
- Subjects
- Female, Humans, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Drug Resistance, Neoplasm, East Asian People, Paclitaxel therapeutic use, Retrospective Studies, Taxoids adverse effects, Taxoids therapeutic use, Hemangiosarcoma drug therapy, Skin Neoplasms drug therapy
- Abstract
Cutaneous angiosarcoma (CAS) is a rare and highly aggressive type of vascular tumor. Although chemoradiotherapy with taxanes is recognized as a first-line therapy for CAS, second-line therapy for CAS remains controversial. From the above findings, the efficacy and safety profiles of taxane-switch (change paclitaxel to docetaxel or vise), eribulin methylate, and pazopanib regimens in second-line chemotherapy were evaluated retrospectively in 50 Japanese taxane-resistant CAS patients. Although there was no significant difference in progression-free survival (P = 0.3528) among the regimens, the incidence of all adverse events (AEs) (P = 0.0386), as well as severe G3 or more AEs (P = 0.0477) was significantly higher in the eribulin methylate group and pazopanib group than in the taxane-switch group. The present data suggest that switching to another taxane should be considered for the treatment of taxane-resistant CAS in second-line therapy based on the safety profiles., (© 2023 Japanese Dermatological Association.)
- Published
- 2023
- Full Text
- View/download PDF
5. Sentinel lymph node biopsy in extramammary Paget disease: A 13-year institutional experience.
- Author
-
Maeda T, Nagai K, Uehara J, Toyoshima R, Nakagawa T, and Yoshino K
- Subjects
- Humans, Sentinel Lymph Node Biopsy, Retrospective Studies, Lymphatic Metastasis diagnosis, Lymphatic Metastasis pathology, Lymph Nodes pathology, Prognosis, Lymph Node Excision, Paget Disease, Extramammary diagnosis, Paget Disease, Extramammary surgery, Paget Disease, Extramammary pathology, Sentinel Lymph Node surgery, Sentinel Lymph Node pathology, Skin Neoplasms diagnosis, Skin Neoplasms surgery
- Abstract
Whether sentinel lymph node biopsy status is a prognostic factor or effective in determining treatment strategies in extramammary Paget disease remains unclear. This study aimed to investigate the significance of sentinel lymph node biopsy in extramammary Paget disease. We retrospectively reviewed the clinical information of previously untreated patients with invasive extramammary Paget disease who underwent wide local excision of the primary tumor and sentinel lymph node biopsy at our hospital between April 2008 and March 2021. Clinical data including the baseline neutrophil-to-lymphocyte ratio and recurrence-free survival were analyzed. Sentinel lymph node metastases were classified as macrometastases and micrometastases, with a cut-off value for sentinel lymph node tumor burden of 2 mm. Univariate and multivariate analyses of factors affecting sentinel lymph node biopsy positivity and recurrence-free survival rates were performed. Overall, 85 patients were included in the analysis. Patients in the sentinel lymph node biopsy-positive group (n = 26) had a significantly higher invasion level and neutrophil-to-lymphocyte ratio. According to multivariate analyses, invasion level and a high neutrophil-to-lymphocyte ratio were independent predictive factors for sentinel lymph node biopsy positivity, and the sentinel lymph node biopsy status was an independent prognostic factor for recurrence-free survival. In conclusion, sentinel lymph node biopsy provides an accurate risk classification and clinical indication for postoperative follow-up in patients with invasive extramammary Paget disease., (© 2022 Japanese Dermatological Association.)
- Published
- 2023
- Full Text
- View/download PDF
6. Neutrophil-to-lymphocyte ratio is a potential prognostic biomarker for extramammary Paget disease: A retrospective study.
- Author
-
Maeda T, Uehara J, Toyoshima R, Nakagawa T, and Yoshino K
- Subjects
- Humans, Prognosis, Retrospective Studies, Leukocyte Count, Lymphocyte Count, Biomarkers, Tumor blood, Lymphocytes pathology, Neutrophils pathology, Paget Disease, Extramammary diagnosis, Paget Disease, Extramammary pathology, Paget Disease, Extramammary therapy
- Abstract
The neutrophil-to-lymphocyte ratio (NLR) is a useful prognostic biomarker for many cancer types. However, the prognostic value of NLR in patients with extramammary Paget disease (EMPD) remains unclear. This study aimed to determine whether NLR is associated with overall survival (OS) in patients with EMPD. In this single-center retrospective case series, we analyzed data of 109 patients with previously untreated EMPD who presented to our hospital. Data on age, sex, primary tumor site, invasion level, presence of lymph node and distant metastases, baseline NLR, and OS were analyzed. The enrolled patients were classified into the metastatic or non-metastatic EMPD groups. The metastatic EMPD group had higher invasion level and NLR value (p < 0.001 and p = 0.005, respectively) compared with the non-metastatic EMPD group. Cox proportional hazards model analysis showed invasion level (p = 0.0093) and NLR value (p = 0.019) to be independent prognostic factors for OS. Notably, multivariate analysis revealed that invasion level (p = 0.045) and NLR value (p = 0.036) were independent prognostic factors for OS in the metastatic EMPD group. The limitations of this study include the small number of participants and its retrospective nature. In conclusion, since NLR can be routinely assessed with high feasibility, it might be a useful biomarker for predicting prognosis in patients with EMPD., (© 2022 Japanese Dermatological Association.)
- Published
- 2022
- Full Text
- View/download PDF
7. Concordance in judgment of clinical borders of basal cell carcinomas in Japanese patients: A preliminary study of JCOG2005 (J-BASE-MARGIN).
- Author
-
Kamimura A, Nakamura Y, Takenouchi T, Matsushita S, Omodaka T, Yamamura K, Uchi H, Yoshikawa S, Yanagisawa H, Ito T, Kiyohara Y, Nakamura Y, Aoki M, Ishizuki S, Oashi K, Miyagawa T, Maeda T, Ogata D, Hatta N, Ohe S, Isei T, Takahashi A, Umeda Y, Yamaguchi B, Ishikawa M, Horimoto K, Fujsawa Y, Uehara J, Shibayama Y, Kiniwa Y, Kawahara Y, Matsuya T, Uhara H, Kato J, Nakamura Y, Murakami T, Namikawa K, Yoshino K, Funakoshi T, Takatsuka S, Matsui Y, Sasaki J, Koga H, Yokota K, Komori T, Fukushima S, and Yamazaki N
- Subjects
- Humans, Japan, Judgment, Margins of Excision, Carcinoma, Basal Cell pathology, Carcinoma, Basal Cell surgery, Head and Neck Neoplasms, Skin Neoplasms diagnosis, Skin Neoplasms pathology, Skin Neoplasms surgery
- Abstract
Basal cell carcinoma is the most common type of skin cancer, and surgical excision with clear margins is the standard of care. Surgical margins are determined based on risk factors (high or low risk) for recurrence according to the National Comprehensive Cancer Network and Japanese basal cell carcinoma guidelines. The clarity of the clinical tumor border (well-defined or poorly defined) is considered a risk factor, and significant discrepancies in the judgment of clinical tumor borders among dermato-oncologists may occur. Therefore, we analyzed the dermato-oncologists' concordance in judging the clinical tumor border of basal cell carcinoma. Forty-seven dermato-oncologists (experts: 37; young trainees: 10) participated in this study. The datasets of clinical and dermoscopic photographs of 79 Japanese cases of head and neck basal cell carcinoma were used to determine the concordance in the judgment of clinical tumor border. The probability of the border that was selected more often was used to calculate the rater agreement rate for each dataset. Correct judgment was defined as a more frequently selected border, and the concordance rate of clarity of clinical tumor border for each dermato-oncologist was calculated based on the definition of the correct judgment. A median concordance rate of 85% or higher for all dermato-oncologists was predefined as an acceptable rate for clinical use. Of the 79 datasets, rater agreement rates were 80-100%, 60-79%, and 51-59% for 55, 19, and five datasets, respectively. The median concordance rate for all dermato-oncologists was 86% (interquartile range: 82-89%). There was no significant difference in the concordance rate between the experts and the trainees (median, 87% vs. 85.5%; p = 0.58). The concordance rates of dermato-oncologists for all datasets were relatively high and acceptable for clinical use., (© 2022 Japanese Dermatological Association.)
- Published
- 2022
- Full Text
- View/download PDF
8. Helicobacter bilis infection induces oxidative stress in and enhances the proliferation of human cholangiocytes.
- Author
-
Yamashita M, Adachi T, Ono S, Yoshino K, Imamura H, Matsushima H, Tanaka T, Kosaka T, Soyama A, Hidaka M, Kanetaka K, and Eguchi S
- Subjects
- Cell Proliferation, Humans, Interleukin-6, Interleukin-8, Reactive Oxygen Species, Helicobacter, Helicobacter Infections complications, Helicobacter Infections genetics, Oxidative Stress
- Abstract
Background: Helicobacter bilis, an enterohepatic Helicobacter species, represents a carcinogenic risk factor for cholangiocytes owing to the prevalence of infections in patients with biliary tract cancer, cholecystitis, and pancreaticobiliary maljunction. However, the effect of H. bilis infection on cholangiocytes and the process and mechanism of carcinogenesis are not known. We aimed to determine the effects of H. bilis on cholangiocytes, focusing on inflammation and oxidative stress., Materials and Methods: Helicobacter bilis and MMNK-1 cells were cocultured for 24 h and inflammatory cytokine secretion was evaluated. Furthermore, MMNK-1 cell proliferation, intracellular reactive oxidant species (ROS) production, and DNA damage caused by ROS were investigated. All factors were compared with and without H. bilis infection., Results: Interleukin (IL)-6 and IL-8 secretion were significantly increased in MMNK-1 cocultures with H. bilis (IL-6, 24.3 ± 12.2 vs. 271.1 ± 286.4 pg/ml; IL-8, 167.6 ± 78.7 vs. 1085.1 ± 1047.1 pg/ml, p < .05). MMNK-1 proliferation was also significantly higher in H. bilis cocultures (1.05 ± 0.02 vs. 1.00-fold, respectively; p < .05). Coculturing enhanced the production of ROS in MMNK-1 cells depending on the cell concentration of H. bilis (1.0 vs. 1.17 ± 0.06, p < .05); however, DNA injury was not observed in cocultures with H. bilis (5.35 ± 0.87 vs. 6.08 ± 0.55 pg/μl, p = .06)., Conclusions: Helicobacter bilis infection induced ROS production in and enhanced the proliferation of cholangiocytes., (© 2022 John Wiley & Sons Ltd.)
- Published
- 2022
- Full Text
- View/download PDF
9. Emergency aortic valve replacement complicated by unmanaged pheochromocytoma.
- Author
-
Tamaki R, Yamasaki M, Nishi H, Yoshino K, Abe K, and Misumi H
- Subjects
- Aortic Valve surgery, Humans, Male, Middle Aged, Adrenal Gland Neoplasms complications, Adrenal Gland Neoplasms surgery, Endocarditis, Bacterial, Heart Valve Prosthesis, Pheochromocytoma complications, Pheochromocytoma surgery
- Abstract
A 53-year-old male undergoing emergency aortic valve replacement for infective endocarditis developed a hypertensive crisis early during the operation. Suspecting a pheochromocytoma, intravenous phentolamine was immediately administered, after which the procedure was completed as scheduled. Although quite rare, a pheochromocytoma can be encountered during emergency open heart surgery; thus, early recognition of abnormal blood pressure change and appropriate management are important. Here, we present details of blood pressure control mainly by use of phentolamine, in this case, to demonstrate effective management of a hypertensive crisis during emergency cardiac surgery because of a pheochromocytoma., (© 2021 Wiley Periodicals LLC.)
- Published
- 2021
- Full Text
- View/download PDF
10. Case series of BRAF-mutated advanced melanoma treated with encorafenib plus binimetinib combination therapy.
- Author
-
Fujimura T, Yoshino K, Kato H, Fujisawa Y, Nakamura Y, Yamamoto Y, Kunimoto K, Ito T, Matsushita S, Maekawa T, Ohuchi K, Amagai R, Muto Y, Furudate S, Kambayashi Y, Hashimoto A, and Aiba S
- Subjects
- Antineoplastic Combined Chemotherapy Protocols therapeutic use, Benzimidazoles, Carbamates, Humans, Mutation, Protein Kinase Inhibitors therapeutic use, Proto-Oncogene Proteins B-raf genetics, Sulfonamides, Melanoma drug therapy, Melanoma genetics, Skin Neoplasms drug therapy, Skin Neoplasms genetics
- Abstract
The efficacy of encorafenib plus binimetinib (E + B) combination therapy for BRAF-mutated advanced melanoma as second-line therapy and beyond is still unknown. In this report, we investigated 22 cases of BRAF-mutated advanced melanoma treated with E + B combination therapy. The objective response rate (ORR) for the total cohort was 68.4%. Notably, the ORR for the second-line and beyond cohort was 73.3%, suggesting that the therapeutic effect of E + B combination therapy is comparable with that of first-line targeted therapy. In contrast, overall survival and progress-free survival in our present cohort was worse than that in a previous clinical trial. Notably, although the incidence rate of severe adverse events was higher than that in a previous report, our present study suggested that E + B combination therapy is a well-tolerated antimelanoma regimen. Our present study suggested that the efficacy and safety profile of E + B combination therapy as a second-line therapy and beyond is comparable with that of first-line targeted therapy., (© 2020 Japanese Dermatological Association.)
- Published
- 2021
- Full Text
- View/download PDF
11. Demographic and clinical characteristics of extramammary Paget's disease patients in Japan from 2000 to 2019.
- Author
-
Ghazawi FM, Iga N, Tanaka R, Fujisawa Y, Yoshino K, Yamashita C, Yamamoto Y, Fujimura T, Yanagi T, Hata H, Matsushita S, Le M, Roy SF, Lagacé F, Ishida Y, Kabashima K, and Otsuka A
- Subjects
- Demography, Humans, Japan epidemiology, Male, Genital Neoplasms, Male, Paget Disease, Extramammary epidemiology
- Published
- 2021
- Full Text
- View/download PDF
12. Pigmented circumscribed plantar hypokeratosis mimicking acral lentiginous melanoma: A case report.
- Author
-
Ohkuma K, Yoshino K, Morita H, Sugimoto E, Hiura A, Uehara J, and Fujisawa Y
- Subjects
- Humans, Melanoma diagnosis, Skin Neoplasms diagnosis
- Published
- 2021
- Full Text
- View/download PDF
13. Immune-related pancytopenia caused by nivolumab and ipilimumab combination therapy for unresectable melanoma of unknown primary.
- Author
-
Uehara J, Yoshino K, Sugiyama E, Ohkuma K, Oaku S, Yamashita C, Hiura A, and Fujisawa Y
- Subjects
- Aged, 80 and over, Biopsy, Bone Marrow drug effects, Bone Marrow immunology, Bone Marrow pathology, CTLA-4 Antigen antagonists & inhibitors, CTLA-4 Antigen immunology, Female, Humans, Melanoma drug therapy, Melanoma immunology, Melanoma secondary, Neoplasms, Unknown Primary drug therapy, Neoplasms, Unknown Primary immunology, Pancytopenia blood, Pancytopenia chemically induced, Pancytopenia immunology, Skin Neoplasms drug therapy, Skin Neoplasms immunology, Skin Neoplasms secondary, T-Lymphocytes, Regulatory drug effects, T-Lymphocytes, Regulatory immunology, Antineoplastic Combined Chemotherapy Protocols adverse effects, Immune Checkpoint Inhibitors adverse effects, Ipilimumab adverse effects, Nivolumab adverse effects, Pancytopenia diagnosis
- Published
- 2020
- Full Text
- View/download PDF
14. Bone metastases could be predictive factors of central nervous system metastases in extramammary Paget's disease.
- Author
-
Yamashita C, Yoshino K, Oaku S, Hiura A, Uehara J, Otsuka A, and Fujisawa Y
- Subjects
- Aged, Aged, 80 and over, Bone Neoplasms mortality, Bone Neoplasms therapy, Female, Follow-Up Studies, Genital Neoplasms, Female mortality, Genital Neoplasms, Female therapy, Genital Neoplasms, Male mortality, Genital Neoplasms, Male therapy, Humans, Infratentorial Neoplasms mortality, Infratentorial Neoplasms secondary, Infratentorial Neoplasms therapy, Male, Middle Aged, Neoplasm Staging, Paget Disease, Extramammary mortality, Paget Disease, Extramammary secondary, Paget Disease, Extramammary therapy, Prognosis, Bone Neoplasms secondary, Genital Neoplasms, Female pathology, Genital Neoplasms, Male pathology, Infratentorial Neoplasms diagnosis, Paget Disease, Extramammary diagnosis
- Published
- 2020
- Full Text
- View/download PDF
15. Dynamics of neutrophil and C-reactive protein reflect the clinical course of pyrexia during combination therapy with dabrafenib and trametinib.
- Author
-
Maeda T, Yoshino K, Yamashita C, Nagai K, Oaku S, Kato M, Hiura A, Uehara J, and Fujisawa Y
- Subjects
- Administration, Oral, Adult, Aged, Biomarkers blood, Feasibility Studies, Female, Fever blood, Fever chemically induced, Fever drug therapy, Humans, Leukocyte Count, Male, Melanoma drug therapy, Middle Aged, Prednisolone administration & dosage, Retrospective Studies, Skin Neoplasms drug therapy, Time Factors, Treatment Outcome, Young Adult, Antineoplastic Combined Chemotherapy Protocols adverse effects, C-Reactive Protein analysis, Fever diagnosis, Imidazoles adverse effects, Neutrophils, Oximes adverse effects, Pyridones adverse effects, Pyrimidinones adverse effects
- Abstract
Pyrexia is the most common adverse event in patients with melanoma or other solid organ malignancies that are treated with the combination of dabrafenib and trametinib (combi-DT). Given the expanded indication for combi-DT, management of pyrexia is a high priority. No previous case series has revealed which blood markers reflect the course of pyrexia and there is no consensus on the management strategy for pyrexia. The current case series study describes the utility of neutrophil count (NC), neutrophil ratio (NR) and C-reactive protein (CRP) in 11 patients with metastatic melanoma and BRAF V600 mutations who experienced pyrexia during combi-DT in our department. We also described the clinical course of pyrexia episodes that were managed with the concomitant use of oral prednisolone and immediate withdrawal of combi-DT. Consequently, the analysis of 37 pyrexia episodes in 11 patients showed that the differences in NC, NR and CRP at the onset of pyrexia were significantly different from those at pyretolysis (P = 0.01, 0.006 and 0.03, respectively). Additionally, in the 24 pyrexia episodes treated with the concomitant use of oral prednisolone and the immediate withdrawal of combi-DT, the mean duration of pyrexia and the mean time to restart combi-DT were 3 and 6 days, respectively. Therefore, the blood markers that reflect the course of pyrexia during combi-DT may be helpful for the appropriate management of pyrexia; also, our management strategy for pyrexia successfully reduced the duration of pyrexia and did not require a long-term drug holiday. Further large-scale studies are required to verify our results., (© 2019 Japanese Dermatological Association.)
- Published
- 2019
- Full Text
- View/download PDF
16. Reply to "Early ambulation versus bedrest after skin grafting in extramammary Paget's disease".
- Author
-
Maeda T, Yoshino K, Nagai K, Oaku S, Kato M, Hiura A, and Hata H
- Subjects
- Bed Rest, Early Ambulation, Humans, Skin, Skin Transplantation, Paget Disease, Extramammary surgery
- Published
- 2019
- Full Text
- View/download PDF
17. Successful outcome of early ambulation after extensive skin grafting in extramammary Paget's disease.
- Author
-
Maeda T, Yoshino K, Nagai K, Oaku S, Kato M, and Hata H
- Subjects
- Aged, Aged, 80 and over, Female, Graft Survival, Humans, Length of Stay statistics & numerical data, Male, Margins of Excision, Middle Aged, Postoperative Complications epidemiology, Postoperative Complications etiology, Time Factors, Treatment Outcome, Early Ambulation methods, Paget Disease, Extramammary surgery, Postoperative Complications prevention & control, Skin Neoplasms surgery, Skin Transplantation adverse effects
- Abstract
Extramammary Paget's disease (EMPD) is a rare cutaneous malignancy that mainly affects the senior population. There is a relatively high risk of postoperative complications from surgery in the senior population, such as disuse syndrome, deep vein thrombosis and postoperative delirium. To prevent such postoperative complications, early ambulation is recommended. However, EMPD often requires extensive skin grafting because of the need for a large resection margin. To avoid skin graft failure, many institutions require that patients have several days of postoperative bedrest. For these reasons, there has been no consensus on standard postoperative rest for EMPD. In this study, we defined 20 patients who walked from the day after surgery as an "early ambulation group" and 23 patients with 5 days postoperative bedrest as a "control group". We evaluated the skin graft survival, postoperative complications and the duration of hospitalization for both groups. Skin graft survival and complications related to the surgical wounds (infection and hemorrhage) in the early ambulation group were found to be comparable with those in the control group. Of note, the other complications (aspiration pneumonia, ileus, delirium, orthostatic hypotension and insomnia) were less frequent (P < 0.001) and the duration of postoperative hospitalization was shorter (P = 0.013) in the early ambulation group than in the control group. Our study suggests that early ambulation after surgery for EMPD does not impair skin graft survival but reduces postoperative complications and the duration of hospitalization., (© 2018 Japanese Dermatological Association.)
- Published
- 2018
- Full Text
- View/download PDF
18. Case of successfully switching from nivolumab to vemurafenib with oral corticosteroids.
- Author
-
Imafuku K, Yoshino K, Yamaguchi K, Tsuboi S, Ohara K, and Hata H
- Subjects
- Administration, Oral, Aged, Betamethasone administration & dosage, Drug Eruptions etiology, Female, Humans, Melanoma drug therapy, Melanoma pathology, Skin Neoplasms drug therapy, Skin Neoplasms pathology, Treatment Outcome, Drug Eruptions prevention & control, Drug Substitution adverse effects, Glucocorticoids administration & dosage, Nivolumab therapeutic use, Vemurafenib therapeutic use
- Published
- 2018
- Full Text
- View/download PDF
19. Two cases of successful treatment for severe skin rash induced by vemurafenib following nivolumab therapy without cessation of vemurafenib.
- Author
-
Tsuboi S, Yoshino K, Yamaguchi K, Imafuku K, and Ohara K
- Subjects
- Adult, Aged, Female, Glucocorticoids therapeutic use, Humans, Prednisolone therapeutic use, Proto-Oncogene Proteins B-raf, Vemurafenib adverse effects, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Exanthema chemically induced, Exanthema prevention & control, Melanoma drug therapy, Nivolumab administration & dosage, Skin Neoplasms drug therapy, Vemurafenib administration & dosage
- Published
- 2017
- Full Text
- View/download PDF
20. Case of primary pleural angiosarcoma with malignant seeding along the pleural tap tract.
- Author
-
Yamaguchi K, Yoshino K, Imafuku K, Tsuboi S, and Ohara K
- Subjects
- Aged, Fatal Outcome, Female, Humans, Pleural Effusion diagnosis, Pleural Effusion etiology, Pleural Effusion therapy, Tomography, X-Ray Computed, Biopsy adverse effects, Hemangiosarcoma pathology, Neoplasm Seeding, Pleural Neoplasms pathology, Thoracoscopy adverse effects
- Published
- 2017
- Full Text
- View/download PDF
21. Nivolumab therapy before vemurafenib administration induces a severe skin rash.
- Author
-
Imafuku K, Yoshino K, Ymaguchi K, Tsuboi S, Ohara K, and Hata H
- Subjects
- Adult, Aged, Antineoplastic Agents therapeutic use, Antineoplastic Agents, Immunological adverse effects, Exanthema diagnosis, Female, Humans, Male, Melanoma drug therapy, Middle Aged, Nivolumab therapeutic use, Severity of Illness Index, Skin Neoplasms drug therapy, Exanthema chemically induced, Nivolumab adverse effects, Vemurafenib therapeutic use
- Published
- 2017
- Full Text
- View/download PDF
22. Pazopanib does not bring remarkable improvement in patients with angiosarcoma.
- Author
-
Kitamura S, Yanagi T, Inamura Y, Hata H, Imafuku K, Yoshino K, and Shimizu H
- Subjects
- Administration, Oral, Aged, Aged, 80 and over, Anemia chemically induced, Chemical and Drug Induced Liver Injury etiology, Disease-Free Survival, Fatigue chemically induced, Female, Humans, Indazoles, Male, Protein Kinase Inhibitors administration & dosage, Protein Kinase Inhibitors adverse effects, Protein-Tyrosine Kinases antagonists & inhibitors, Pyrimidines administration & dosage, Pyrimidines adverse effects, Retrospective Studies, Sulfonamides administration & dosage, Sulfonamides adverse effects, Thrombocytopenia chemically induced, Treatment Outcome, Hemangiosarcoma drug therapy, Protein Kinase Inhibitors therapeutic use, Pyrimidines therapeutic use, Sulfonamides therapeutic use
- Abstract
Pazopanib is a potent and selective multi-targeted tyrosine kinase inhibitor that has been reported to extend progression-free survival in cases of metastatic soft-tissue sarcoma. However, the efficacy of pazopanib for cutaneous angiosarcoma has not been confirmed. We report eight cases of angiosarcoma treated with pazopanib, and review the efficacy and safety of pazopanib therapy. We retrospectively investigated the clinical information, including age, sex, body surface area, location, performance status, lung or pleural metastasis, preceding treatment, oral dose of pazopanib, response rate, progression-free survival and adverse effects. Five of the eight patients needed to stop the pazopanib treatment due to severe adverse effects, including thrombocytopenia, anemia, drug-associated pancreatitis, acute fulminant hepatitis and general fatigue. Progression-free survival ranged 0.5-3.5 months (mean ± standard deviation, 1.81 ± 1.03). Overall survival ranged 3-26 months (14.13 ± 9.47). Six of the eight cases showed progressive disease, and two of the eight cases showed stable disease. To assess overall survival in angiosarcoma treated with pazopanib, we compared the pazopanib-treated group (n = 8) with the non-pazopanib-treated control group (n = 10). There was no significant difference between two groups (P = 0.19, log-rank test). In conclusion, our case series suggests that pazopanib does not bring remarkable improvement in patients with angiosarcoma., (© 2016 Japanese Dermatological Association.)
- Published
- 2017
- Full Text
- View/download PDF
23. Lymphopenia can be a useful biomarker of adverse events related to vemurafenib.
- Author
-
Imafuku K, Yoshino K, Ishiwata K, Ymaguchi K, Tsuboi S, Ohara K, and Hata H
- Subjects
- Adult, Aged, Female, Humans, Male, Middle Aged, Vemurafenib, Antineoplastic Agents adverse effects, Biomarkers metabolism, Indoles adverse effects, Lymphopenia physiopathology, Sulfonamides adverse effects
- Published
- 2017
- Full Text
- View/download PDF
24. A single-institution study examining cutaneous and non-cutaneous melanomas treated with nivolumab.
- Author
-
Imafuku K, Yoshino K, Ishiwata K, Otobe S, Tsuboi S, Ohara K, and Hata H
- Subjects
- Adult, Aged, Female, Humans, Male, Middle Aged, Nivolumab, Antibodies, Monoclonal therapeutic use, Antineoplastic Agents therapeutic use, Melanoma drug therapy, Skin Neoplasms drug therapy
- Published
- 2016
- Full Text
- View/download PDF
25. Severe rash associated with vemurafenib administration following nivolumab therapy.
- Author
-
Imafuku K, Yoshino K, Ishiwata K, Otobe S, Tsuboi S, Ohara K, and Hata H
- Subjects
- Adult, Antibodies, Monoclonal administration & dosage, Antineoplastic Combined Chemotherapy Protocols administration & dosage, Female, Humans, Indoles administration & dosage, Lung Neoplasms secondary, Melanoma pathology, Nivolumab, Skin Neoplasms pathology, Sulfonamides administration & dosage, Vemurafenib, Antibodies, Monoclonal adverse effects, Antineoplastic Combined Chemotherapy Protocols adverse effects, Exanthema chemically induced, Indoles adverse effects, Melanoma drug therapy, Skin Neoplasms drug therapy, Sulfonamides adverse effects
- Published
- 2016
- Full Text
- View/download PDF
26. Usefulness of docetaxel as first-line chemotherapy for metastatic extramammary Paget's disease.
- Author
-
Yoshino K, Fujisawa Y, Kiyohara Y, Kadono T, Murata Y, Uhara H, Hatta N, Uchi H, Matsushita S, Takenouchi T, Hayashi T, and Ohara K
- Subjects
- Aged, Aged, 80 and over, Docetaxel, Humans, Japan epidemiology, Male, Middle Aged, Paget Disease, Extramammary mortality, Retrospective Studies, Antineoplastic Agents therapeutic use, Paget Disease, Extramammary drug therapy, Taxoids therapeutic use
- Abstract
In invasive extramammary Paget's disease (EMPD), distant metastases may develop and the condition may become fatal; however, no standardized treatment has been established. Although based on only a few cases, several chemotherapy regimens were reported to be promising. We conducted a multicenter, retrospective study to evaluate the efficacy of docetaxel for metastatic EMPD. We retrospectively collected data on 18 metastatic EMPD patients treated using docetaxel from 1998 to 2012 in 12 institutes in Japan. The following clinical data were collected: tumor response, time to progression, overall survival and adverse effects. Of those, three patients treated combined with S-1, one patient treated with weekly schedule and one patient treated combined with radiotherapy were excluded from the further analysis. All 13 patients received monthly docetaxel as the first-line treatment. The average number of treatment cycles was 9.1. Among the 12 patients with a confirmed response, seven (58%) showed a partial response, three (25%) stable disease and two (17%) progressive disease. The disease control rate (partial response + stable disease) was as high as 83%. The time to progression and median overall survival were 7.1 and 16.6 months, respectively. The 1-year overall survival rate determined by the Kaplan-Meier method was 75.0%. All adverse effects were manageable and no treatment-related deaths were observed. The high disease control rate and overall survival shown by this study suggest that first-line use of docetaxel may be a promising treatment for metastatic EMPD. A prospective clinical trial is required to confirm our results., (© 2015 Japanese Dermatological Association.)
- Published
- 2016
- Full Text
- View/download PDF
27. Hepatitis C virus NS5A protein interacts with lysine methyltransferase SET and MYND domain-containing 3 and induces activator protein 1 activation.
- Author
-
Chen M, Gan X, Yoshino K, Kitakawa M, Shoji I, Deng L, and Hotta H
- Subjects
- Cell Line, Cytoplasm metabolism, HSP90 Heat-Shock Proteins metabolism, Hepacivirus genetics, Hepatitis C virology, Histone-Lysine N-Methyltransferase biosynthesis, Host-Pathogen Interactions, Humans, Protein Interaction Mapping methods, Protein Interaction Maps, Replicon physiology, Sequence Analysis, Protein, Sequence Deletion, Viral Nonstructural Proteins biosynthesis, Viral Nonstructural Proteins genetics, Virus Replication physiology, Hepacivirus metabolism, Histone-Lysine N-Methyltransferase metabolism, Transcription Factor AP-1 biosynthesis, Transcription Factor AP-1 metabolism, Viral Nonstructural Proteins metabolism
- Abstract
Hepatitis C virus (HCV) non-structural protein 5A (NS5A) is a multifunctional protein that is involved in the HCV life cycle and pathogenesis. In this study, a host protein(s) interacting with NS5A by tandem affinity purification were searched for with the aim of elucidating the role of NS5A. An NS5A-interacting protein, SET and MYND domain-containing 3 (SMYD3), a lysine methyltransferase reportedly involved in the development of cancer, was identified. The interaction between NS5A and SMYD3 was confirmed in ectopically expressing, HCV RNA replicon-harboring and HCV-infected cells. The other HCV proteins did not bind to SMYD3. SMYD3 bound to NS5A of HCV genotypes 1b and 2a. Deletion mutational analysis revealed that domains II and III of NS5A (amino acids [aa] 250 to 447) and the MYND and N-SET domains of SMYD3 (aa 1 to 87) are involved in the full extent of NS5A-SMYD3 interaction. NS5A co-localized with SMYD3 exclusively in the cytoplasm, thereby inhibiting nuclear localization of SMYD3. Moreover, NS5A formed a complex with SMYD3 and heat shock protein 90 (HSP90), which is a positive regulator of SMYD3. The intensity of binding between SMYD3 and HSP90 was enhanced by NS5A. Luciferase reporter assay demonstrated that NS5A significantly induces activator protein 1 (AP-1) activity, this being potentiated by co-expression of SMYD3 with NS5A. Taken together, the present results suggest that NS5A interacts with SMYD3 and induces AP-1 activation, possibly by facilitating binding between HSP90 and SMYD3. This may be a novel mechanism of AP-1 activation in HCV-infected cells., (© 2016 The Societies and John Wiley & Sons Australia, Ltd.)
- Published
- 2016
- Full Text
- View/download PDF
28. Expression of CD70 in nasal natural killer/T cell lymphoma cell lines and patients; its role for cell proliferation through binding to soluble CD27.
- Author
-
Yoshino K, Kishibe K, Nagato T, Ueda S, Komabayashi Y, Takahara M, and Harabuchi Y
- Subjects
- Adult, Antibodies immunology, CD27 Ligand immunology, CD27 Ligand metabolism, Cell Line, Tumor, Cytotoxicity, Immunologic, Female, Gene Expression, Humans, Lymphoma, Extranodal NK-T-Cell immunology, Lymphoma, Extranodal NK-T-Cell metabolism, Male, Middle Aged, Nose Neoplasms immunology, Nose Neoplasms metabolism, Protein Binding, RNA, Viral genetics, RNA, Viral metabolism, Tumor Necrosis Factor Receptor Superfamily, Member 7 genetics, Tumor Necrosis Factor Receptor Superfamily, Member 7 metabolism, Viral Matrix Proteins genetics, Viral Matrix Proteins metabolism, CD27 Ligand genetics, Lymphoma, Extranodal NK-T-Cell genetics, Nose Neoplasms genetics
- Abstract
Nasal natural killer (NK)/T cell lymphoma (NNKTL) is associated with Epstein-Barr virus (EBV). The present study analysed gene expression patterns of the NNKTL cell lines SNK6, SNK1 and SNT8, which are positive for EBV and latent membrane protein (LMP)-1, using a complementary DNA array analysis. We found that CD70 was specifically expressed in SNK6 and SNT8. Reverse transcription polymerase chain reaction and flow cytometric analyses confirmed that CD70 was expressed in all 3 NNKTL cell lines, but not in the other EBV-positive NK-cell lines. In vitro studies showed that NNKTL cell lines proliferated, in a dose-dependent fashion, in response to exogenous soluble CD27, which is the ligand for CD70. In NNKTL patients, we confirmed that the CD70 was expressed on the lymphoma cells in NNKTL tissues and that soluble CD27 was present in sera at higher levels as compared to healthy individuals. Finally, complement-dependent cytotoxicity assay showed that anti-CD70 antibody mediated effective complement-dependent killing of NNKTL cells and the affected target CD70 expression on the cells. These results suggest that CD70 acts as a functional receptor binding to soluble CD27, resulting in lymphoma progression and that immunotherapy using anti-CD70 antibody may be a potential candidate for treatment for NNKTL., (© 2012 Blackwell Publishing Ltd.)
- Published
- 2013
- Full Text
- View/download PDF
29. Perspectives on a new educational system for dental hygiene students in Japan.
- Author
-
Hikiji H, Kubota K, Takahashi Y, Kanehisa Y, Yoshino K, Sonoki K, Chiwata K, Inoue H, Tsujisawa T, Akiifusa S, Kakinoki Y, Nishihara T, and Fukuta J
- Subjects
- Education, Professional standards, Humans, Japan, Licensure, Specialties, Dental education, Time Factors, Curriculum, Dental Hygienists education, Education, Professional organization & administration, Education, Professional trends
- Abstract
The purpose of this article is to report changes to dental hygiene education in Japan and to evaluate the successful implementation of these changes in 2010. The legislative change that began in 2005 revised the length of education for dental hygiene students from two years to three or four years (the mandate was three years), which has led to a dramatic change in program curriculum. After a five-year moratorium, a new curriculum has been established for dental hygiene education in Japan. The new curriculum provides students the requisite knowledge to effectively perform the latest dental hygiene procedures. Although the change of the educational system from the present mandatory three-year to the new four-year programs poses many administrative problems, we believe this shift will ultimately provide a more thorough and in-depth education for students.
- Published
- 2011
30. Neuronal mechanism of mirror movements caused by dysfunction of the motor cortex.
- Author
-
Tsuboi F, Nishimura Y, Yoshino-Saito K, and Isa T
- Subjects
- Animals, Brain Mapping, Electromyography, Electrophysiology, Female, Hand physiopathology, Macaca, Male, Movement Disorders physiopathology, Motor Activity physiology, Motor Cortex physiopathology, Movement physiology
- Abstract
Mirror movements (MMs) are often observed in hemiplegic patients after stroke, and are supposed to reflect some aspects of their recovery process. Therefore, understanding the neuronal mechanism of MMs is important, but from the currently available evidence in human case studies, the mechanism of MMs has not been clearly understood. Here we found that in monkeys, after reversible inactivation of the right primary motor cortex (M1) by microinjection of muscimol, MMs were induced in the right hand during voluntary grasping with the left hand, which were partially affected by the injection. Using this animal model, we investigated the origin of MMs after dysfunction of the M1. We found the MMs thus induced were completely abolished by additional blockade of the left M1. Electromyogram (EMG) activities in some homonymous muscle pairs in bilateral hands were co-activated. Detailed analysis of EMG activities suggested that the enhanced activation of the left M1, which led to MMs in the right hand, was not directly driven by the activity of the right M1, whose activity was likely to be affected by the injection. Rather, the present finding has suggested that common drive of bilateral M1 from higher-order structures and reduction in commissural inhibition from the affected side concomitantly enhanced the activity of the cortico-motoneuronal pathway of the intact side, and led to the MMs., (© 2010 The Authors. European Journal of Neuroscience © 2010 Federation of European Neuroscience Societies and Blackwell Publishing Ltd.)
- Published
- 2010
- Full Text
- View/download PDF
31. A clinical study of Japanese patients with ulcer induced by low-dose aspirin and other non-steroidal anti-inflammatory drugs.
- Author
-
Nakashima S, Arai S, Mizuno Y, Yoshino K, Ando S, Nakamura Y, Sugawara K, Koike M, Saito E, Naito M, Nakao M, Ito H, Hamaoka K, Rai F, Asakura Y, Akamatu M, Fujimori K, Inao M, Imai Y, Ota S, Fujiwara K, and Shiibashi M
- Subjects
- Adult, Aged, Anti-Inflammatory Agents, Non-Steroidal administration & dosage, Aspirin administration & dosage, Endoscopy, Gastrointestinal, Female, Helicobacter Infections complications, Helicobacter pylori, Hemostasis, Endoscopic, Humans, Male, Middle Aged, Peptic Ulcer Hemorrhage chemically induced, Prospective Studies, Recurrence, Anti-Inflammatory Agents, Non-Steroidal adverse effects, Anti-Ulcer Agents therapeutic use, Aspirin adverse effects, Histamine H2 Antagonists therapeutic use, Peptic Ulcer Hemorrhage prevention & control
- Abstract
Background: The incidence and severity of non-steroidal anti-inflammatory drugs (NSAIDs)-induced gastro-duodenal ulcer have not been extensively studied in Japan., Aim: We performed a prospective study to clarify NSAIDs-induced gastro-duodenal injury, focusing especially on low-dose aspirin (L-A)., Methods: Two hundred and thirty-eight patients with bleeding peptic ulcers admitted to our hospital. History of taking NSAIDs and anti-ulcer drugs was obtained from all patients who underwent endoscopic examinations. The lesion scores of patients taking L-A were classified numerically from zero (no lesion) to five (ulcer)., Results: The NSAIDs were associated with 28.2% of hemorrhagic ulcers. The rates of patients using L-A, loxoprofen, diclofenac, and combination of two of these drugs were 27, 16, 10 and 9%, respectively. Co-administered anti-ulcer drugs were cytoprotective anti-ulcer drugs (27%), H2 receptor antagonists (16%), PPI (4%), and none (53%). In patients taking L-A, H2 receptor antagonists were used most frequently. The HP was positive in 63% of L-A-induced ulcer cases and in 69% of NSAIDs other than low-dose aspirin-induced ulcer cases. The lesion scores of patients taking L-A with H2 receptor antagonists or PPI were significantly lower than those of patients who were taking only L-A (P < 0.05)., Conclusions: Approximately one-third of hospitalized patients with NSAIDs-induced hemorrhagic ulcer showed an association with L-A. Prospective randomized controlled trials including H2 receptor antagonists are required to establish preventive efforts aimed at L-A-induced gastro-duodenal injury.
- Published
- 2005
- Full Text
- View/download PDF
32. Properties and plasticity of the primate somatosensory and motor cortex related to orofacial sensorimotor function.
- Author
-
Sessle BJ, Yao D, Nishiura H, Yoshino K, Lee JC, Martin RE, and Murray GM
- Subjects
- Animals, Face innervation, Motor Cortex physiology, Motor Neurons physiology, Mouth innervation, Neuronal Plasticity physiology, Neurons, Afferent physiology, Primates physiology, Somatosensory Cortex physiology
- Abstract
1. The lateral pericentral region of the cerebral cortex has been well documented in primates to be important in sensorimotor integration and control and in the learning of new motor skills. 2. The present article provides, first, an overview of limb sensorimotor cortical mechanisms and, second, outlines recent evidence pointing to an important role for the face sensorimotor cortex in semi-automatic, as well as trained, orofacial motor behaviour and to its propensity for neuroplastic changes in association with orofacial motor skill acquisition or an altered oral environment.
- Published
- 2005
- Full Text
- View/download PDF
33. A metalloproteinase inhibitor prevents acute graft-versus-host disease while preserving the graft-versus-leukaemia effect of allogeneic bone marrow transplantation.
- Author
-
Hattori K, Hirano T, Miyajima H, Yamakawa N, Ikeda S, Yoshino K, Tateno M, Oshimi K, Kayagaki N, Yagita H, and Okumura K
- Subjects
- Animals, Female, Flow Cytometry, Glycine therapeutic use, Humans, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Transplantation, Homologous, Bone Marrow Transplantation methods, Glycine analogs & derivatives, Graft vs Host Disease prevention & control, Graft vs Tumor Effect drug effects, Hydroxamic Acids therapeutic use, Protease Inhibitors therapeutic use
- Abstract
We examined the effect of a hydroxamic acid-based matrix metalloproteinase inhibitor (KB-R7785), which we previously demonstrated to have a potent ameliorating effect on acute graft-versus-host disease (GVHD), and on the graft-versus-leukaemia (GVL) effect of allogeneic bone marrow transplantation (BMT). KB-R7785 was administered to (C57BL/6 x BALB/c) F1 (CBF1) mice that had been inoculated with IgE-producing B53 hybridoma cells of BALB/c origin as a model tumour, along with or without transplantation of C57BL/6 (B6) bone marrow cells and spleen cells (BMS). Administration of KB-R7785 without BMS significantly prolonged the survival of B53-inoculated CBF1 mice by inhibiting the infiltration of B53 cells into the liver and spleen. Transplantation of B6 BMS without KB-R7785 resulted in the death of most recipients due to acute GVHD while efficiently eliminating B53 cells. Administration of KB-R7785 along with B6 BMS resulted in a 50% survival of B53-inoculated CBF1 mice over 50 d without histological manifestations of acute GVHD or residual B53 cells. These results indicate the beneficial effects of KB-R7785 that inhibit tumour infiltration and prevent acute GVHD while preserving the GVL effect of allogeneic BMT.
- Published
- 1999
34. In vitro assessment of a chemically synthesized Shiga toxin receptor analog attached to chromosorb P (Synsorb Pk) as a specific absorbing agent of Shiga toxin 1 and 2.
- Author
-
Takeda T, Yoshino K, Adachi E, Sato Y, and Yamagata K
- Subjects
- Bacterial Toxins genetics, Diatomaceous Earth metabolism, Escherichia coli genetics, Humans, Organosilicon Compounds chemical synthesis, Shiga Toxin 1, Shiga Toxin 2, Trisaccharides chemical synthesis, Tumor Cells, Cultured, Bacterial Toxins metabolism, Escherichia coli metabolism, Glycolipids metabolism, Organosilicon Compounds metabolism, Receptors, Cell Surface metabolism, Trisaccharides metabolism
- Abstract
A synthetic analog of Shiga toxin (Stx) receptor (Synsorb Pk) was quantitatively assessed to determine whether it can protect human renal adenocarcinoma cells (ACHN cells) from the cytotoxicity of Stx1 and Stx2 by coincubation experiments. Coincubation of 100 and 20 ng of Stxl and Stx2 with 50 mg of Synsorb Pk for 1 hr at 37 C in 1 ml of Eagle's Minimum Essential Medium supplemented with 1% (v/v) non-essential amino acid and 10% (v/v) fetal calf serum protected 50% of the cells from the cytotoxic effect. Chromosorb P, an inert matrix control, did not absorb the Stxs at all. Heat-treatment (boiled for 10 min) to Synsorb Pk caused a 50% decrease in Stx2-binding activity, but did not effect the Stx1 binding. Further, Stxs bound to Synsorb Pk could be demonstrated. When 20 mg of Synsorb Pk was coincubated for 30 min at 37 C in 1 ml of phosphate-buffered saline with 1 and 10 ng or more of Stx1 or Stx2, respectively, the toxins could be detected on the surface when the bound toxins on Synsorb Pk were used as the solid phase in enzyme immunoassay. The amount of 100 ng/ml of both Stxl and Stx2 appeared to saturate 20 mg/ml of Synsorb Pk after coincubating for 30 min at 37 C. While assessing the Stxs' binding activity to Synsorb Pk, it was demonstrated that Stxl had a higher affinity to Pk trisaccharide than Stx2. These observations provide useful information on the effectiveness of Synsorb Pk to trap and eliminate free Stxs produced in the gut of patients infected by Stx-producing Escherichia coli, and to prevent the progression of hemorrhagic colitis to hemolytic uremic syndrome.
- Published
- 1999
- Full Text
- View/download PDF
35. Antibody response against the Epstein-Barr virus-coded nuclear antigen2 (EBNA2) in nasopharyngeal carcinoma.
- Author
-
Chatani M, Teshima T, Inoue T, Yoshino K, Ikegami N, Hirai K, and Shimakage M
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Epstein-Barr Virus Nuclear Antigens, Female, Follow-Up Studies, Herpesvirus 4, Human immunology, Humans, Male, Middle Aged, Nasopharyngeal Neoplasms pathology, Nasopharyngeal Neoplasms therapy, Antibodies, Viral analysis, Antigens, Viral immunology, Nasopharyngeal Neoplasms immunology
- Abstract
Specific antibody responses against the Epstein-Barr virus-coded nuclear antigen2 (EBNA2) were evaluated. Thirty-five sera from pretreatment patients of nasopharyngeal carcinoma (NPC) and 12 from healthy adults were tested. Although the anti-EBNA2 response did not show any correlation with T stage, overall stage, or histopathology, it showed a correlation with the N stage of the disease. In a serological follow-up study, 17 (85%) of 20 patients showed a correlation on the change of antibody levels to EBNA2 and clinical progression. This suggests that EBNA2 serology might represent a useful marker relative to tumor status.
- Published
- 1991
- Full Text
- View/download PDF
36. An analysis of factors influencing the isolation rate of herpes simplex virus.
- Author
-
Tada A, Sekine N, Toba M, and Yoshino K
- Subjects
- Animals, Culture Media, Freezing, Herpes Simplex diagnosis, Humans, Hydrogen-Ion Concentration, Methods, Micropore Filters, Penicillins pharmacology, Rabbits, Simplexvirus drug effects, Streptomycin pharmacology, Virus Cultivation, Herpes Simplex microbiology, Simplexvirus isolation & purification
- Abstract
Attempts were made to improve the rate of isolation of herpes simplex virus (HSV) from clinical specimens by minimizing loss of virus infectivity during transportation and employing the most sensitive cells for isolation. Basical analyses using standard strains of type 1 and type 2 HSV indicated that virus titer decrease was marked even at low temperatures in environments free of proteinous stabilizer such as normal serum or tissue extract, negating the generally held concept that HSV is stable in distilled water. YLE (Earle-lactalbumin HYDROLYSATE-YEAST EXTRACT) medium containing 20% inactivated calf serum was determined to be a transport medium of choice, because degradation of suspended virus during storage and freeze-thawing was negligible and loss of virus during Millipore filtration was minimal. Special coating of the membrane could also be obviated by the use of this solution. In a cell susceptibility test using clinical specimens, secondary rabbit kidney (SRK) cells were the most sensitive, showing a quick development of cytopathic effect. Vero and RK-13 cells were the second best, whereas monkey kidney, HeLa and L cells were far less sensitive. A total of 136 specimens from suspected cases, sent by dermatologists, were tested using SRK cells, and 99 strains of type 1 and 15 strains of type 2 HSV were isolated. Excluding one case from which vaccinia virus was isolated, the isolation rate of HSV was 84.4%.
- Published
- 1977
- Full Text
- View/download PDF
37. Rapid identification and typing of herpes simplex virus in clinical specimens by a direct microneutralization test.
- Author
-
Hayashi Y, Hidano A, Yoshino K, and Abe K
- Subjects
- Herpes Simplex microbiology, Humans, Microbiological Techniques, Simplexvirus classification, Neutralization Tests methods, Simplexvirus isolation & purification
- Published
- 1982
- Full Text
- View/download PDF
38. Differences in the biochemical activity in hairless mouse skin and other organs after systemic and topical methotrexate treatment.
- Author
-
Yoshino K and Maibach HI
- Subjects
- Administration, Topical, Animals, Female, Injections, Intraperitoneal, Mice, Mice, Hairless, DNA biosynthesis, Intestine, Small metabolism, Methotrexate administration & dosage, Skin metabolism, Spleen metabolism
- Abstract
DNA synthesis in epidermis, spleen, small intestine, and muscle of the hairless mouse was measured after systemic and topical methotrexate dosing. Mice intraperitoneally injected with methotrexate (5 mg/Kg) incorporated 3H-UdR in epidermis at 90% of baseline at 3 hrs, and the incorporation was not suppressed at 24 hrs. Muscle DNA synthesis was not suppressed. In the spleen and small intestine, incorporation was greatly suppressed to 10% at 3 hrs. On the other hand, after topically applied methotrexate (25 mg/Kg) treatment, epidermis and muscle were 60-90% of baseline at 3-24 hrs and spleen and small intestine were 5-10% at 3-6 hrs. In a prolonged time schedule study (4 days), epidermal incorporation after intraperitoneally applied methotrexate (5 mg/Kg) showed no suppression, but was instead stimulated to 180% at 2 days. 5-Fluorouracil, a thymidine kinase inhibitor in antitumor agents, was topically applied (25 mg/Kg) and compared for DNA synthesis. The incorporation of 3H-UdR was drastically suppressed at 3 hrs. The results suggest that methotrexate is percutaneously absorbed, but does not extensively suppress epidermal DNA synthesis in hairless mice, although it does suppress spleen and intestinal DNA synthesis.
- Published
- 1989
- Full Text
- View/download PDF
39. Studies on the neutralization of herpes simplex virus. IX. Variance in complement requirement among IgG and IgM from early and late sera under different sensitization conditions.
- Author
-
Yoshino K and Isono N
- Subjects
- Antigen-Antibody Reactions, Dose-Response Relationship, Immunologic, Immunoglobulin G, Immunoglobulin M, Kinetics, Antibodies, Viral, Complement Activation, Simplexvirus immunology
- Published
- 1978
- Full Text
- View/download PDF
40. Studies on the neutralization of herpes simplex virus. XI. Differences between slow-reacting complement-requiring neutralizing (s-CRN) antibodies in IgG and IgM.
- Author
-
Yoshino K, Urayama M, and Abe K
- Subjects
- Animals, Antibodies, Anti-Idiotypic immunology, Antibody Formation, Complement Fixation Tests, Dose-Response Relationship, Immunologic, Hot Temperature, Neutralization Tests, Rabbits, Time Factors, Antibodies, Viral immunology, Immunoglobulin G immunology, Immunoglobulin M immunology, Simplexvirus immunology
- Abstract
Late IgG and IgM from a rabbit immunized with herpes virus were tested for ordinary neutralizing (N) antibody, complement-requiring neutralizing (CRN) antibody and in addition CRN antibody detectable by overnight sensitization at 0 C (s-CRN antibody). Heat stability tests showed that IgG s-CRN antibody was slightly less resistant to heating at 70 C than were N and CRN antibodies, whereas all three activities of IgM were quickly degraded at this temperature. Dose-response curves with varying amounts of complement (C) or anti-antibody revealed a marked difference between IgG s-CRN and IgM s-CRN antibodies. While 1-hr sensitization at 37 C was insufficient to detect IgG s-CRN antibody, it had the same effect as overnight sensitization at 0 C for IgM s-CRN antibody. When sensitization at 0 C was prolonged to 3 days, unexpectedly high endpoints exceeding 1:10,000 were obtained even with IgM. consequently, enhancement by C was several hundred-fold with IgM in contrast to 5- to 10-fold enhancement of IgG s-CRN antibody, which was similar to that after overnight sensitization. Also IgM obviously required more C than did IgG. These results suggest that IgM of late serum is slower reacting and more C-dependent than IgG s-CRN antibody. Tests with early rabbit sera indicated that the s-CRN antibody detectable by 3-day sensitization reaches a high level before the appearance of N antibody.
- Published
- 1980
- Full Text
- View/download PDF
41. Efficacy of intradermal administration of herpes simplex virus subunit vaccine.
- Author
-
Yoshino K, Yanagi K, and Abe K
- Subjects
- Animals, Freund's Adjuvant, Guinea Pigs, Injections, Intradermal, Injections, Intraperitoneal, Injections, Subcutaneous, Vaccination, Viral Vaccines immunology, Antibodies, Viral biosynthesis, Simplexvirus immunology, Viral Vaccines administration & dosage
- Published
- 1982
- Full Text
- View/download PDF
42. Studies on the neutralization of herpes simplex virus. X. Demonstration of complement-requiring neutralizing (CRN) and slow-reacting CRN (s-CRN) antibodies in late IgG.
- Author
-
Yoshino K, Isono N, Tada A, and Urayama M
- Subjects
- Animals, Cold Temperature, Female, Kinetics, Neutralization Tests, Seeds microbiology, Time Factors, Antibodies, Viral immunology, Complement System Proteins immunology, Immunoglobulin G immunology, Simplexvirus immunology
- Abstract
A sample of late IgG from a rabbit hyperimmunized with herpes simplex virus was analyzed for neutralizing (N) and complement-requiring neutralizing (CRN) antibodies. In a usual endpoint test, N and CRN titers were 1: 40 and 1: 160, respectively, but when virus-IgG mixtures were incubated at 0 C overnight before addition of complement (C), an endpoint of 1:1280 was obtained. Virus sensitized at 0 C overnight required more C for inactivation than did sensitized virus formed earlier. Sensitization kinetic curve experiments employing a proper initial virus concentration, which permitted differentiation of sensitized viruses requiring different amounts of C, indicated that formation of sensitized virus detectable only with a relatively large amount of C proceeded slowly at IgG dilutions where the ordinary CRN antibody requiring a smaller amount of C was negligible. The results strongly suggested that the IgG sample contained slow-reacting CRN (s-CRN) antibody in excess of the hitherto known CRN antibody. As to the mechanism of formation of s-CRN complexes, experiments failed to prove the occurrence of complexes initially insensitive to C, and it appears more likely that s-CRN antibody has a comparatively low avidity for virus.
- Published
- 1979
- Full Text
- View/download PDF
43. A micro-neutralization system for detection of s-CRN antibody to herpes simplex virus.
- Author
-
Yoshino K and Abe K
- Subjects
- Animals, Cell Line, Chlorocebus aethiops, Complement System Proteins, Cross Reactions, Neutralization Tests methods, Simplexvirus growth & development, Time Factors, Viral Plaque Assay, Antibodies, Viral analysis, Simplexvirus immunology
- Abstract
It was learned that the ordinary micro-neutralization system with herpes simplex virus (HSV) gave a composite result of the initial neutralization and the effect of antibody on subsequent growth of unneutralized virus. In the case of slow-reacting complement-requiring neutralizing (s-CRN) antibody, which was detected by incubating virus-serum mixtures at 4 C for 3 days before addition of complement, the titer obtained was lower than expected from the result of the plaque reduction test. This was thought ascribable to its low ability to prevent viral breakthrough caused by growth of unneutralized virus. This was overcome by adding an appropriate amount of hyperimmune antibody at 3 hr after addition of cells. The endpoint of s-CRN antibody so determined was but slightly lower than that obtained by the plaque reduction test. Early (1-week) rabbit sera, which were negative in the ordinary micro-neutralization test, titered 1:2,560 to 1:5,120 when tested by this method. When the 3-day sensitization in the cold was substituted by 5-hr incubation at 37 C, the titer obtained was 2 to 4-fold lower; in this case, however, the whole process could be finished within 3 days. Also, s-CRN antibody reactive with type 2 HSV in homologous and heterologous sera could be detected by the same method using type 1 hyperimmune serum as the additional antibody.
- Published
- 1981
- Full Text
- View/download PDF
44. Studies on the neutralization of herpes simplex virus. VIII. Significance of viral sensitization for inactivation by complement.
- Author
-
Yoshino K, Hashimoto M, and Shinkai K
- Subjects
- Antibodies, Viral, Immune Sera, Immunoglobulin G analysis, Complement System Proteins, Neutralization Tests, Simplexvirus immunology
- Abstract
Early and late IgG of rabbits immunized with herpes virus showed, respectively, 8-fold and 2-fold enhancement of neutralization endpoint in the presence of complement (C). Kinetic curve experiments employing an appropriate amount of virus revealed that both neutralization and sensitization followed first-order reaction, and each IgG possessed a certain range of concentration where neutralization was negligible while sensitization was marked. Dose responses of neutralization and sensitization velocities demonstrated that the C enhancement of late IgG was about 7-fold and that of early IgG more than 20-fold. These facts suggested that the IgGs contained two different entities of complement-requiring (CRN) and non-requiring neutralizing (N) antibodies at different proportions, only the former being responsible for sensitization. The different CRN: N ratios obtained by the endpoint and kinetic methods may mean either that the two antibodies differ in avidity for the virus or that the number of critical sites per virion for CRN antibody is greater than that for N antibody. In this interpretation, sensitization by CRN antibody as well as neutralization by N antibody is thought to result from attachment of a single antibody molecule to the viral critical site. Alternative explanations, ascribing the mechanism of neutralization to steric hindrance of critical sites or to multiple hit of those sites by antibody, were denied by analyses of the present data.
- Published
- 1977
- Full Text
- View/download PDF
45. A simplified disc technique for quantitating mouse DNA, RNA and protein synthesis: circadian rhythms.
- Author
-
Yoshino K, Oba K, and Maibach HI
- Subjects
- Animals, Carbon Radioisotopes, Epidermis metabolism, Female, Mice, Mice, Hairless, Tritium, Circadian Rhythm, DNA biosynthesis, Protein Biosynthesis, RNA biosynthesis
- Published
- 1986
- Full Text
- View/download PDF
46. Estimation of type-specific neutralizing antibody to herpes simplex virus type 2 in uterine cervical cancer patients by a new absorption method.
- Author
-
Kawana T and Yoshino K
- Subjects
- Absorption, Acute Disease, Adult, Aged, Animals, Female, Genital Diseases, Female immunology, Humans, Middle Aged, Neutralization Tests, Pregnancy, Rabbits, Uterine Cervical Neoplasms etiology, Antibodies, Viral biosynthesis, Antibody Specificity, Herpes Simplex immunology, Uterine Cervical Neoplasms immunology
- Abstract
A simple method of estimating type-specific neutralizing antibody to type 2 herpes simplex virus (HSV-2) was devised with the use of the microneutralization system. Serially diluted serum was mixed in the well with a constant amount of type 1 virus (HSV-1), and after 3 days' incubation at 37 C, the plate was irradiated with ultraviolet light. The absorbing HSV-1 consisted of culture fluid plus an extract of infected Vero cells not especially concentrated. The well then received indicator HSV-1 or HSV-2, and after being left at 37 C for 1 hr a suspension of dispersed Vero cells was dropped into the wells, following our standard neutralization procedure. Preliminary tests with rabbit antisera showed that even a low level of HSV-2 antibody was detected by this method, unless an exceptionally high titer of HSV-1 antibody originally coexisted with the HSV-2 antibody. Sera from acutely infected persons testified to the specificity of the antibody so detected. It was revealed by means of the new technique that the rate of HSV-2 antibody was significantly higher in uterine cervical cancer patients than in control women. There was no correlation between the clinical stage of cervical cancer and the presence of HSV-2 antibody.
- Published
- 1980
- Full Text
- View/download PDF
47. A new microplate neutralization test for typing of herpes simplex virus.
- Author
-
Tada A and Yoshino K
- Subjects
- Animals, Antibody Specificity, Complement Activation, Dose-Response Relationship, Immunologic, Guinea Pigs, Serotyping methods, Simplexvirus immunology, Simplexvirus classification
- Abstract
A microplate serum neutralization test for estimation of complement-requiring neutralizing (CRN) antibody was established as the first step for simplification of typing of herpes simplex virus (HSV). When guinea pigs were immunized with type 2 HSV, the late sera could mostly differentiate the types of HSV better than hyperimmune rabbit sera, the CRN titer against the heterologous type 1 HSV being much lower than the homologous titer. Sera of guinea pigs immunized with type 1 HSV showed about the same level of cross reaction against type 2 HSV as did rabbit antisera. Guinea pig sera having minimal levels of cross reaction were selected, and their high dilution (1:160) and complement were added to serial 10-fold dilutions of virus in the microplate titration of virus infectivity. Selective reduction of virus titer by either antiserum could determine the type of HSV. No equivocal intermediate case was found among a number of stock strains including many fresh isolates. The typing result coincided with that determined by a modification of Yang et al's method based on virus titers obtained with Vero and primary chick embryo cells. The typing based on plaquing in chick embryo cells sometimes failed to identify type 1 HSV.
- Published
- 1978
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.