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Fluoroborodipyrrole (BODIPY) and Tetraphenylethylene (TPE) are well‐known molecules in the realm of optical materials, celebrated for their exceptional properties. Recent research increasingly focuses on their combined use. In this study, a novel non‐synthetic method is taken to harness the synergistic therapeutic potential stemming from intermolecular interactions by employing nanoparticle encapsulation and core–shell structure. In vitro and in vivo experiments results show that the nanoparticles encapsulating BODIPY and TPE exhibit low cytotoxicity, efficient antitumor properties, and excellent visualization, achieving remarkable 1+1>2 effects. This innovative method not only provides high therapeutic efficacy, but also reduces time and economic costs, providing a novel perspective for exploring the combination of BODIPY and TPE as well as similar molecules in the field of optical materials. [ABSTRACT FROM AUTHOR]

Antarctic krill oil (AKO) is highly sought after by consumers and the food industry due to its richness in a variety of nutrients and physiological activities. However, current extraction methods are not sufficient to better extract AKO and its nutrients, and AKO is susceptible to lipid oxidation during processing and storage, leading to nutrient loss and the formation of off-flavors and toxic compounds. The development of various extraction methods and encapsulation systems for AKO to improve oil yield, nutritional value, antioxidant capacity, and bioavailability has become a research hotspot. This review summarizes the research progress of AKO from extraction to encapsulation system construction. The AKO extraction mechanism, technical parameters, oil yield and composition of solvent extraction, aqueous enzymatic extraction, supercritical/subcritical extraction, and three-liquid-phase salting-out extraction system are described in detail. The principles, choice of emulsifier/wall materials, preparation methods, advantages and disadvantages of four common encapsulation systems for AKO, namely micro/nanoemulsions, microcapsules, liposomes and nanostructured lipid carriers, are summarized. These four encapsulation systems are characterized by high encapsulation efficiency, low production cost, high bioavailability and high antioxidant capacity. Depending on the unique advantages and conditions of different encapsulation methods, as well as consumer demand for health and nutrition, different products can be developed. However, existingAKOencapsulation systems lack relevant studies on digestive absorption and targeted release, and the single product category of commercially available products limits consumer choice. In conjunction with clinical studies of AKO encapsulation systems, the development of encapsulation systems for special populations should be a future research direction. [ABSTRACT FROM AUTHOR]

We propose a novel high‐performance dual‐resonance enhanced photoacoustic spectroscopy (DRE‐PAS) gas sensor based on a highly sensitive fiber optic cantilever beam microphone and a high‐Q spherical photoacoustic cell (PAC). The first‐order resonant frequency (FORF) of the spherical PAC is analyzed by finite element analysis to match the FORF of the cantilever microphone for the double resonance enhancement of the photoacoustic signal. The photoacoustic spectroscopy (PAS) system, including the DRE‐PAS sensor, a 1532.8 nm distributed feedback laser, and a high‐speed spectrometer, has been successfully exploited for trace acetylene (C2H2) detection. The experimental results show that the limit of detection (LOD) is 106.8 parts‐per‐billion (ppb) with an integral time of 1 s, and the LOD can be further reduced to 11.03 ppb by Allan‐Werle deviation for 100 s integral time. The normalized noise equivalent absorption coefficient can be obtained as 2.44 × 10−8 cm−1 WHz−1/2. The reported DRE‐PAS gas sensor has the superior characteristics of photoacoustic signal enhancement, high sensitivity, and strong antielectromagnetic interference capability, which can provide a new solution for PAS development. [ABSTRACT FROM AUTHOR]

Aim: To investigate the linkage of matrix metalloproteinase (MMP) gene polymorphisms with the pathogenesis of knee osteoarthritis (OA). Methods: This meta‐analysis study systematically retrieved relevant studies from PubMed, Embase, the Cochrane Central, Wanfang Data, CNKI, and SinoMed up to November 2020. Odds ratios (ORs) and 95% confidence intervals (CIs) were used to estimate the association between MMP gene polymorphisms and OA. Results: A total of nine case–control studies comprising 1719 knee OA patients and 1904 controls were included in this meta‐analysis. The results revealed that MMP‐1‐1607 (rs1799750) 1G/2G polymorphism was not significantly associated with knee OA risk in four genetic models (OR (95% CI): allele model: 0.89 (0.57, 1.40), p =.615); dominant mode: 0.82 (0.47, 1.44), p =.486; recessive model: 0.88 (0.49, 1.57), p =.659; homozygote model: 0.79 (0.34, 1.82), p =.576. The association was significant for dominant model of MMP‐3 C/T: 1.54 (1.10–2.15), p =.013, especially in Asian ethnicity (1.63 (1.11, 2.39), p =.013). Variants of MMP‐13 C/T polymorphism were associated with increased risk of knee OA development based on dominant model: 1.56 (1.19, 2.06), p =.001 and homozygote model: 2.12 (1.44, 3.13), p <.001, and there were significant associations between MMP‐13 C/T polymorphism and knee OA risk in Asian ethnicity under different genetic models (all p >.05). Conclusions: Present evidence suggested that the gene polymorphisms of MMP‐1‐1607 1G/2G may not be associated with the risk of OA. But, the dominant model of MMP‐3 and MMP‐13 polymorphisms in Asian ethnicity was significantly correlated with knee OA. [ABSTRACT FROM AUTHOR]

Background: Breast phyllodes tumours (PTs) are a unique type of fibroepithelial neoplasms with metastatic potential and recurrence tendency. However, the precise nature of heterogeneity in breast PTs remains poorly understood. This study aimed to elucidate the cell subpopulations composition and spatial structure and investigate diagnostic markers in the pathogenesis of PTs. Methods: We applied single‐cell RNA sequencing and spatial transcriptomes on tumours and adjacent normal tissues for integration analysis. Immunofluorescence experiments were conducted to verify the tissue distribution of cells. Tumour cells from patients with PTs were cultured to validate the function of genes. To validate the heterogeneity, the epithelial and stromal components of tumour tissues were separated using laser capture microdissection, and microproteomics data were obtained using data‐independent acquisition mass spectrometry. The diagnostic value of genes was assessed using immunohistochemistry staining. Results: Tumour stromal cells harboured seven subpopulations. Among them, a population of widely distributed cancer‐associated fibroblast‐like stroma cells exhibited strong communications with epithelial progenitors which underwent a mesenchymal transition. We identified two stromal subpopulations sharing epithelial progenitors and mesenchymal markers. They were inferred to further differentiate into transcriptionally active stromal subpopulations continuously expressing COL4A1/2. The binding of COL4A1/2 with ITGA1/B1 facilitated a growth pattern from the stroma towards the surrounding glands. Furthermore, we found consistent transcriptional changes between intratumoural heterogeneity and inter‐patient heterogeneity by performing microproteomics studies on 30 samples from 11 PTs. The immunohistochemical assessment of 97 independent cohorts identified that COL4A1/2 and CSRP1 could aid in accurate diagnosis and grading. Conclusions: Our study demonstrates that COL4A1/2 shapes the spatial structure of stromal cell differentiation and has important clinical implications for accurate diagnosis of breast PTs. [ABSTRACT FROM AUTHOR]

Although the literature advocates that the state plays a significant role in affecting firms' behaviour in emerging economies, whether state participation promotes or impedes firms' innovation decisions requires further scholarly attention. In this study, we develop a framework in which we examine how and under what conditions state participation affects firms' green innovation. Results from publicly listed firms in China show an inverted U‐shaped relationship between state participation and green innovation, so that state participation in the form of minority ownership is most effective for firms seeking to conduct green innovation. This curvilinear relationship is negatively moderated by firm age and firm size, such that the inverted U‐shape flattens when a firm is older and larger. The relationship is positively moderated by subnational institutional development such that the inverted U‐shape steepens when institutional development is higher. These findings provide useful implications for resource dependence theory in explaining whether state participation serves as a facilitator of or a burden on firms' green innovation. [ABSTRACT FROM AUTHOR]

Objectives: Analyzing the lower limb coronal morphological parameters in populations without knee osteoarthritis (KOA) holds significant value in predicting, diagnosing, and formulating surgical strategies for KOA. This study aimed to comprehensively analyze the variability in these parameters among Chinese non‐KOA populations, employing a substantial sample size. Methods: A cross‐sectional retrospective analysis was performed on the Chinese non‐KOA populations (n = 407; 49.9% females). The study employed an in‐house developed artificial intelligence software to meticulously assess the coronal morphological parameters of all 814 lower limbs. The parameters evaluated included the hip‐knee‐ankle angle (HKAA), weight‐bearing line ratio (WBLR), joint line convergence angle (JLCA), mechanical lateral‐proximal‐femoral angle (mLPFA), mechanical lateral‐distal‐femoral angle (mLDFA), mechanical medial‐proximal‐tibial angle (mMPTA), and mechanical lateral‐distal‐tibial angle (mLDTA). Differences in these parameters were compared between left and right limbs, different genders, and different age groups (with 50 years as the cut‐off point). Results: HKAA and JLCA exhibited left–right differences (left vs. right: 178.2° ± 3.0° vs. 178.6° ± 2.9° for HKAA, p = 0.001; and 1.8° ± 1.5° vs. 1.4° ± 1.6° for JLCA, p < 0.001); except for the mLPFA, all other parameters show gender‐related differences (male vs. female: 177.9° ± 2.8° vs. 179.0° ± 3.0° for HKAA, p < 0.001; 1.5° ± 1.5° vs. 1.8° ± 1.7° for JLCA, p = 0.003; 87.1° ± 2.1° vs. 88.1° ± 2.1° for mMPTA, p < 0.001; 90.2° ± 4.0° vs. 91.1° ± 3.2° for mLDTA, p < 0.001; 38.7% ± 12.9% vs. 43.6% ± 14.1% for WBLR, p < 0.001; and 87.7° ± 2.3° vs. 87.4° ± 2.7° for mLDTA, p = 0.045); mLPFA increase with age (younger vs. older: 90.1° ± 7.2° vs. 93.4° ± 4.9° for mLPFA, p < 0.001), while no statistical difference exists for other parameters. Conclusions: There were differences in lower limb coronal morphological parameters among Chinese non‐KOA populations between left and right sides, different genders, and age. [ABSTRACT FROM AUTHOR]

High‐precision spectral detection and broadband wavelength sensors have attracted considerable interest for important application in color image sensing. Due to the absorption limitations of silicon, conventional complementary metal–oxide–semiconductors have a relatively limited wavelength region from the ultraviolet to near‐infrared spectrum. Here, a simple‐structured device is developed by assembling two back‐to‐back monolayer graphene (MLG)/Si/MLG heterojunctions for high‐resolution wavelength sensing applications. The single MLG/Si/MLG photodetector exhibits a responsivity of 0.18 A W−1, a specific detectivity of 1.36 × 109 Jones, and an external quantum efficiency of 28.5%. Thanks to the unique device geometry, the distribution of photo‐generation rates in the two photodetectors is completely different, which brings about different photoelectric properties. It is found that the relationship between the photocurrent ratio of both photodetectors and the light wavelength can be fitted by a monotonic function, according to which the wavelength of incident light in the range from 260 to 1050 nm can be accurately estimated. A high resolution of 1 nm is demonstrated across a broadband wavelength from 260 to 1000 nm. This good resolution, along with good repeatability and stability, endow the present device with potential promise in future spectral sensing applications. [ABSTRACT FROM AUTHOR]

This study adopts the social capital and organizational learning perspectives to examine (i) how managerial business and political ties drive green product and process innovation, respectively, and (ii) the roles that explorative and exploitative market learning play in moderating the effects of managerial business and political ties on green product and process innovation. Findings from a survey of 283 Chinese firms show that managerial business and political ties distinctively drive green product and process innovation. Explorative and exploitative market learning strengthens the positive effects of managerial business and political ties on green product and process innovation for sustainable development. These findings advance the green innovation literature by clarifying how firms convert top‐level managers' network ties and market‐learning capabilities to strengthen corporate green innovation practices. [ABSTRACT FROM AUTHOR]

Summary: While location information sharing technology provides convenience for unmanned driving and journey navigation, user journey information sharing has also become a disaster for privacy information leakage. The traditional differential privacy method can only perturb the data entirely and cannot consider the design of data availability. In this paper, the difference privacy algorithm is improved by combining it with the Apriori algorithm, and the relevant perturbation is carried out after mining the associated data of the user's trip. In the face of possible data attacks, the privacy protection of the sensitive information of the user's actual data is ensured while the availability of the data is ensured. By testing 3000 trip data generated by experimental simulation, the results show that the correlation information between the original datasets is destroyed. However good availability is maintained after the Laplace data perturbation of the proposed algorithm for both simultaneous and multi‐person trips. [ABSTRACT FROM AUTHOR]

Although starvation stress can alter the homeostasis of mitochondria and promote autophagy, there is still a lack of research focusing on the connection between them. In this study, we found that, accompanied by the upregulation of autophagy flux, the membrane mitochondrial potential (MMP), the content of reactive oxygen species (ROS), the production of ATP, and the copy number of mitochondrial DNA (mt‐DNA) were changed when limiting amino acids supply. We screened and analyzed altered genes related to mitochondrial homeostasis under starvation stress and verified that the expression of mitochondrial transcription factor A (TFAM) was prominently upregulated. Inhibition of TFAM led to the change of mitochondrial function and homeostasis, caused the decrease of SQSTM1 mRNA stability and ATG101 protein level and restricted the autophagy process of cells under amino acid deficient conditions. In addition, the TFAM knockdown and starvation treatment aggravated the DNA damage and reduced proliferation rate of tumor cells. Therefore, our data shows the correlation between mitochondria homeostasis and autophagy, reveals the effect of TFAM on autophagy flux under starvation stress and provides experimental basis for the combined starvation therapy targeting mitochondria to inhibit tumor growth. [ABSTRACT FROM AUTHOR]

To take advantage of the respective strengths of aggregation‐induced emission (AIE) and aggregation‐caused quenching (ACQ) materials, in this paper a unique approach is taken which is that tetraphenylethene and triphenylamine (classical AIEs), borondipyrromethene and benzothiadiazole (classical ACQs) are selected to construct AIE@ACQ bimolecular systems by simple compounding method, focusing on the effects on optical properties such as fluorescence, photothermal therapy, phosphorescence, and photodynamic therapy. Above all, the mechanisms and laws of the system are preliminarily found. First, AIEs and ACQs must, respectively, have electron‐acceptor and electron‐donors to form an intermolecular push–pull structure to ensure that the two molecules are close to each other, and intermolecular π–π stacking interaction separates the highest occupied molecular orbitals and lowest unoccupied molecular orbitals to reduce ΔEst, causing intriguing optical properties. Second, the matching degree of the conjugate structure between AIEs and ACQs is also an important factor. Utilizing these systems, it is found that different structural compositions possess different optical synergy properties, so different AIE@ACQ bimolecular systems can be constructed according to the actual needs, which have the advantages of efficiency and convenience, providing a new idea for the united application of AIEs and ACQs. [ABSTRACT FROM AUTHOR]

Environmental strategies—reactive environmental strategy (RES) and proactive environmental strategy (PES)—emerged as an effective tool for firms to respond to various stakeholders' pressures and mounting environmental issues. Despite their vital role, scant studies have examined how and why firms facing the same institutional environment adopt heterogonous, instead of homogeneous, strategies for achieving corporate sustainable development. To fill this research gap, this study anchored to resource dependence theory investigates how organizational learning (both exploitative and explorative) and social ties (both political and business) independently and interactively affect the adoption of environmental strategies. Using a sample of 272 Chinese firms, the data are analyzed through the SPSS 21, and the proposed hypotheses are tested through moderated hierarchical regression analyses. The findings reveal that firms that engage in exploitative learning tend to adopt RES rather than PES, whereas firms that engage in explorative learning are more likely to adopt PES as opposed to RES. Moreover, firms that are involved in exploitative learning benefit to a greater extent from political ties than from business ties as a means of adopting RES, whereas firms that engage in explorative learning benefit to a greater extent from business ties than from political ties to adopt RES. Our study adds value to the environmental management and strategy literature by clarifying the underlying mechanisms through which firms adopt distinct environmental strategies. [ABSTRACT FROM AUTHOR]

Release of promoter‐proximally paused RNA Pol II into elongation is a tightly regulated and rate‐limiting step in metazoan gene transcription. However, the biophysical mechanism underlying pause release remains unclear. Here, we demonstrate that the pausing and elongation regulator SPT5 undergoes phase transition during transcriptional pause release. SPT5 per se is prone to form clusters. The disordered domain in SPT5 is required for pause release and gene activation. During early elongation, the super elongation complex (SEC) induces SPT5 transition into elongation droplets. Depletion of SEC increases SPT5 pausing clusters. Furthermore, disease‐associated SEC mutations impair phase properties of elongation droplets and transcription. Our study suggests that SEC‐mediated SPT5 phase transition might be essential for pause release and early elongation and that aberrant phase properties could contribute to transcription abnormality in diseases. Synopsis: The super elongation complex (SEC) induces phase transition of SPT5 from pausing clusters into elongation droplets. Disease‐associated SEC mutations impair phase properties of the elongation droplets and transcription. SPT5 has an intrinsic property to form clusters, and its disordered domain is required for pause release and gene activation.SEC promotes SPT5 relocation from pausing clusters into elongation droplets.Diseases‐associated SEC mutations alter the phase properties of the elongation droplets [ABSTRACT FROM AUTHOR]

Designing an imine‐based fluorescent probe capable of greatly suppressing the tendency of intrinsic hydrolysis reaction is an attractive topic in the field of chemo‐/biosensing. In this work, hydrophobic 1,1'‐binaphthyl‐2,2'‐diamine containing two amine groups was introduced to synthesize probe R‐1 bearing two imine bonds linked by two salicylaldehyde (SAs). The hydrophobicity of binaphthyl moiety and the unique clamp‐like structure formed from double imine bonds and from ortho‐OH on SA part make probe R‐1 is able to function as an ideal receptor to coordinate with Al3+ ions, leading to the fluorescence originated from the complex rather than from the assumed hydrolyzed fluorescent amine is turned on. Further study revealed that, when Al3+ ions were introduced, both the hydrophobic binaphthyl moiety and the clamp‐like double imine structure in the designed imine‐based probe showed important contributions to suppress the intrinsic hydrolysis reaction, resulting in generating a stable coordination complex with an extremely high selectivity in fluorescence response. [ABSTRACT FROM AUTHOR]

Despite the dominant discourses on the important role of entrepreneurial leadership in firms' development, related theoretical and empirical studies are still lacking in the environmental management area. Adopting upper echelons theory, this study develops a moderated mediation model that examines how entrepreneurial leadership affects the adoption of green innovation (GI) through organizational learning culture and how environmental dynamism moderates the mediating effect. This theoretical model is tested using a sample of 248 Chinese firms. Findings support the positive impact of entrepreneurial leadership on GI and the mediating role of organizational learning culture. The moderated mediation path analysis shows that environmental dynamism moderates the indirect effect of entrepreneurial leadership on GI via organizational learning culture. Overall, this study adds to entrepreneurship and environmental management scholarship by providing novel insights into the key role of entrepreneurial leadership in embracing GI. [ABSTRACT FROM AUTHOR]

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Background: Lethal neonatal rigidity and multifocal seizure syndrome (RMFSL, OMIM 614498) is a rare autosomal recessive disease characterized by the onset of rigidity and intractable seizures at or soon after birth. The BRAT1 has been identified to be the disease‐causing gene for RMFSL. This study aimed to determine the underlying pathogenic mutations of a Chinese family with RMFSL and to confirm the effect of the splice‐site mutation by reverse transcription analysis. Methods: Detailed family history and clinical data were recorded, and peripheral blood samples were collected from all available family members. Whole exome sequencing (WES), Sanger sequencing, and bioinformatics analysis were performed to investigate the causative variants. The impact of the intronic variant on splicing was subsequently analyzed by RT‐PCR analysis. Results: We identified two compound heterozygous variants in the BRAT1, c.431‐2A>G in intron 3 and c.1359_1361del(p.Leu454del) in exon 9 in the proband, one inherited from each parent. Furthermore, the 3′‐splice site acceptor (c.431‐2A>G) variant was found to activate a cryptic acceptor splice site, which resulted in the loss of 29 nucleotides and generation of a premature stop codon at code 180, producing a truncated BRAT1 (c.432_460del; p.Ala145Argfs*36). Conclusions: This research identified two mutations in the BRAT1 of one Chinese family with RMFSL. These data can aid in developing clinical diagnoses as well as providing genetic counseling and prenatal interventions to the family. These findings also expand our knowledge of the spectrum of BRAT1 pathogenic variants in RMFSL syndrome. [ABSTRACT FROM AUTHOR]

Hyperlipidemia has been highlighted as one of the most prominent and global chronic conditions nowadays. Bidens bipinnata L. (BBL), a folk medicine in contemporary China, has efficacy in the treatment of hyperlipidemia (HLP) in China. Although some physiological and pathological function parameters of hyperlipidemia have been investigated, little information about the changes in small metabolites in biofluids has been reported. In the present study, global metabolic profiling with high‐performance liquid chromatography–linear ion trap/Orbitrap high‐resolution mass spectrometry (HPLC–LTQ/Orbitrap MS) combined with a pattern recognition method was performed to discover the underlying lipid‐regulating mechanisms of BBL on hyperlipidemic rats induced by high‐fat diet (HFD). The total of four metabolites, up‐ or down‐regulated (p < 0.05 or 0.01), were identified and contributed to the progression of hyperlipidemia. These promising identified biomarkers underpin the metabolic pathway, including glyoxylate and dicarboxylate metabolism, the TCA cycle, sphingolipid metabolism and purine metabolism. They are disturbed in hyperlipidemic rats, and are identified using pathway analysis with MetPA. The altered metabolite indices could be regulated closer to normal levels after BBL intervention. The results demonstrated that urinary metabolomics is a powerful tool in the clinical diagnosis and treatment of hyperlipidemia to provide information on changes in metabolite pathways. [ABSTRACT FROM AUTHOR]

Background: Teacher–student relationships have been linked to various aspects of students' school functioning, including social‐emotional well‐being in school, but the underlying mechanisms need more investigation. Aims: In this study, we analysed longitudinal data to test if students' classroom behavioural engagement was a potential mechanism of change that explained how teacher–student relationships affect student school satisfaction. Sample: We used an archival dataset with a sample of seventh graders (ages 11–14, Mage = 12.7 year) in a middle school in the Southeastern United States. Methods: Adolescents completed self‐report surveys across three waves over the course of 18 months. Results: Longitudinal structural equation modelling analyses revealed that teacher–student relationships were positively associated with positive classroom engagement behaviours and school satisfaction, respectively, at each time, and positive classroom behaviours at Time 2 fully mediated the longitudinal association between teacher–student relationships (Time 1) and school satisfaction (Time 3). Conclusions: Taken together, results suggested that fostering positive teacher‐student relationships to increase students' positive classroom behaviours could be an effective pathway to promote students' satisfaction with school. The applications of the results in educators' and psychologists' work, such as consultation and trainings with teachers, are discussed. [ABSTRACT FROM AUTHOR]

Amides are ubiquitous in physical and life sciences. Given the significant abundance of arenes, dearomative aminocarbonylation of arenes would lead to a large and underexplored chemical space for amide discovery. However, such reactions are challenging due to the high degree of resonance stabilization and selectivity issues. Herein, we disclose an unprecedented dearomative trifluoromethylative aminocarbonylation of arenes via bifunctional coordination to chromium, providing a modular platform for the construction of amides possessing trifluoromethyl (CF3) groups and three‐dimensional rings. Its versatility further enabled a switchable difluoromethylation or trifluoromethylation aminocarbonylation of arene C−H bonds. A possible mechanism was proposed based on control experiments. Finally, the synthetic utility was well demonstrated by diverse applications in the total synthesis of CF3‐functionalized amide‐type drugs, including praziquantel, nateglinide, maraviroc and alloyohimbane. [ABSTRACT FROM AUTHOR]

Background: Diabetes confers an increased risk of fracture. However, whether prediabetes is also a risk factor of osteoporotic fracture has not been comprehensively examined. We performed a meta-analysis to evaluate the relationship between prediabetes and osteoporotic fracture risk.Methods: This meta-analysis included relevant prospective cohort studies from Medline, Embase, and Web of Science databases. A random-effect model after incorporation of the intra-study heterogeneity was selected to pool the results. Subgroup analyses were applied to evaluate the influences of study characteristics on relationship between prediabetes and osteoporotic fracture risk.Results: Eight studies including 33,136 community dwelling adult patients were included, and 7429 (22.4%) patients were prediabetic. Prediabetes was not independently associated with a higher risk of osteoporotic fracture compared with normoglycemia (adjusted risk ratio: 1.03, 95% confidence interval: 0.88-1.21, P = 0.69, I2  = 42%). Sensitivity limited to the elderly population showed consistent results (RR: 1.10, 95% CI: 0.91-1.24, P = 0.15, I2  = 0%). Subgroup analysis suggested that prediabetes defined by HbA1c (approximately 5.7%-6.4%) was associated with a higher risk of osteoporotic fracture (RR: 1.24, 95% CI: 1.01-1.53, P = 0.04), but not that defined by impaired fasting glucose or impaired glucose tolerance (P = 0.60). Sex, follow-up duration, and adjustment of bone mineral density did not significantly affect the outcome.Conclusions: Current evidence does not support that prediabetes is independently associated with osteoporotic fracture risk. Different definitions of prediabetes may affect the association between prediabetes and osteoporotic fracture risk. [ABSTRACT FROM AUTHOR]

Optically pure sulfoxides are noteworthy compounds applied in a wide range of industrial fields; however, the biocatalytic deracemization of racemic sulfoxides is challenging. Herein, a high‐enantioselective methionine sulfoxide reductase A (MsrA) was combined with a low‐enantioselective styrene monooxygenase (SMO) for the cyclic deracemization of sulfoxides. Enantiopure sulfoxides were obtained in >90 % yield and with >90 % enantiomeric excess (ee) through dynamic "selective reduction and non‐selective oxidation" cycles. The cofactors of MsrA and SMO were subsequently regenerated by the cascade catalysis of three auxiliary enzymes through the consumption of low‐cost D‐glucose. Moreover, this "one‐pot, one‐step" cyclic deracemization strategy exhibited a wide substrate scope toward various aromatic, heteroaromatic, alkyl and thio‐alkyl sulfoxides. This system proposed an efficient strategy for the green synthesis of chiral sulfoxide. [ABSTRACT FROM AUTHOR]

Objective: At present, the true sagittal alignment of the cervical spine is uncertain, resulting in no standard reference for subaxial cervical surgery. So, we aimed to explore the age difference of normal cervical sagittal alignment and to further investigate the mid‐and long‐term changes of sagittal alignment after subaxial cervical spine surgery. Materials and Methods: This was a retrospective study and 1223 asymptomatic volunteers and 79 patients undergoing subaxial cervical spine surgery were retrospectively reviewed in total. Asymptomatic volunteers and patients were divided into six subgroups: 20–29, 30–39, 40–49, 50–59, 60–69 and ≥70 groups. The age difference and trend with age of cervical sagittal parameters of asymptomatic volunteers were assessed by cervical lateral radiography and analyzed by ANOVA test, and the regression equation of C2‐7 Cobb was established via multiple linear regression. Based on the C2‐7 Cobb regression equations of different ages, the theoretical value, deviation value, loss value of the C2‐7 Cobb, and JOA recovery rate of patients were calculated, and the correlation among the loss value, deviation value of the C2‐7 Cobb, and JOA recovery rate of the 79 patients was evaluated by Pearson correlation analysis. Results: For the asymptomatic volunteers, the C0‐2 Cobb decreased gradually with increasing age. The C2‐7 Cobb, C2‐7 SVA, T1S, NT, and TIA increased gradually with increasing age. The CBVA fluctuated with increasing age. T1S demonstrated a moderate correlation with C2‐7 Cobb (r = 0.60, p < 0.01); C0‐2 Cobb, C2‐7 SVA, CBVA, and TIA demonstrated a fair correlation with C2‐7 Cobb (r = −0.30, −0.33, 0.41, 0.40, p < 0.01); age demonstrated a poor correlation with C2‐7 Cobb (r = 0.19, p < 0.01). The regression equations of C2‐7 Cobb were established using C0‐2 Cobb, C2‐7 SVA, CBVA, and T1S. For the patients with subaxial cervical spine surgery, the loss of C2‐7 Cobb was moderately correlated with the deviation of C2‐7 Cobb (r = 0.33, p < 0.01). Conclusion: The age difference of cervical sagittal alignment was obvious, and the C2‐7 Cobb increased with age especially. The closer the postoperative C2‐7 Cobb was to the theoretical value of corresponding age, the smaller the loss of correction angle was, and the better the mid‐ and long‐term outcomes. The personalized sagittal reconstruction should be performed according to age difference for subaxial cervical spine surgery. [ABSTRACT FROM AUTHOR]

Glyphosate‐based herbicides (GBHs) are the most widely used pesticide in the world, and its extensive use has increased pressures on environmental safety and potential human and livestock health risks. This study investigated the effects of GBHs on antioxidant capacity, inflammatory cytokines, and autophagy of porcine intestinal epithelial cells (IPEC‐J2) and its molecular mechanism. Also, the protective effects of N‐acetylcysteine (NAC) against the toxicity of GBHs were evaluated. Our results showed that the activities of antioxidant enzymes (SOD, GSH‐Px) were decreased by GBHs. GBHs increased inflammatory factors (IL‐1β, IL‐6, TNF‐α) and the mRNA expression of iNOS and COX‐2. GBHs induced the up‐regulation of Nrf2/HO‐1 pathway and the phosphorylation of IκB‐α and NFκB p65, up‐regulation of LC3‐II/LC3‐I, and down‐regulation of P62, and NFκB inhibitor decreased the mRNA expression of inflammatory cytokines (IL‐1β, IL‐6, IL‐8). Moreover, NAC reduced the cytotoxicity by suppressing ROS levels, and changed the autophagy‐related proteins such as the suppression of LC3‐II conversion and up‐regulation of P62. Our findings unveil a novel mechanism of GBHs effects on IPEC‐J2 cells and NAC can reverse cytotoxicity to some extent. [ABSTRACT FROM AUTHOR]

N-α-Acetyltransferase 10 (NAA10) was reported to be involved in tumour invasion and metastasis in several of tumours. However, the role and mechanism of NAA10- mediated invasion and metastasis in oral squamous cell carcinoma (OSCC) remains undetermined. Herein, our study showed that NAA10 inhibits cell migration and invasion in vitro and attenuates the xenograft tumorigenesis in nude mice. Mechanistically, we demonstrated that there is a physical interaction between NAA10 and RelA/p65 in OSCC cells, thereby preventing RelA/p65-mediated transcriptional activation of Pirh2. Consequently, inhibition of Pirh2 increased p53 level and suppressed the expression of p53 downstream targets, matrix metalloprotein-2 (MMP-2) and MMP-9. Therefore, NAA10 may function as a tumour metastasis suppressor in the progression of OSCC by targeting Pirh2-p53 axis and might be a prognostic marker as well as a therapeutic target for OSCC. [ABSTRACT FROM AUTHOR]

The injection of high‐pressure fluid and the propagation of hydraulic fractures (HF) can impact the stress state of natural fractures (NF) in shale reservoirs, which will lead to shear slip in NF that are in a critical stress state. However, the NF mechanics response mechanism during hydraulic fracture (HF) approximation has not been well investigated, especially under triaxial stress and injection conditions. This work focuses on the NF shear‐slip characteristics during HF through experimental investigation. Each shale sample contains a single rough NF in the tests, and high‐pressure fluid is injected through a pre‐drilled hole at the top of the sample. Meanwhile, the deformation (axial deformation and radial deformation), axial stress, and injection pressure of the sample during the experiment were recorded in real‐time. The experimental results show that the NF will initiate in tension or shear mode during the HF approaching process, and the fracture will produce shear slip in both initiation modes. In addition, the deviator stress, confining pressure, hydraulic fracture approach angle, and fracture surface roughness all affect the degree of shear slip in natural fractures (NF). The results further reveal the mechanical response mechanism of NF during hydraulic fracturing and provide a reference for optimizing hydraulic fracturing process measures. [ABSTRACT FROM AUTHOR]

A novel dual‐band dual‐polarization Fabry‐Pérot resonator antenna (FPRA) is presented, which is composed of double‐layer frequency selective surfaces (FSS1 and FSS2) and planar patch antenna. By analyzing the passband and stopband characteristics of the FSS1 and the FSS2, it is revealed that FSS1 has stopband characteristic for low frequency and passband characteristic for high frequency, while FSS2 shows stopband characteristic for high frequency and passband characteristic for low frequency. Based on these traits, the gain is improved by the high‐reflection coefficient of FSS1 at low frequency. The linear to circular polarization (CP) conversion is achieved by FSS2 at high frequency. To verify the performance of this proposed design, a prototype FPRA is fabricated and measured. The measurement results show that the peak gain of antenna reaches 16.1 dBic with CP at 9.4 GHz and 15.3 dBi with linear polarization at 5.6 GHz. The measured 3‐dB gain bandwidths is from 5.35 GHz to 5.8 GHz in the lower band, and the combined fractional bandwidth (−10 dB impedance matching, 3‐dB measured gain, and 3‐dB axial ratio) from 9.27 to 9.55 GHz in the upper band is achieved. [ABSTRACT FROM AUTHOR]

Circular RNAs (circRNAs) are a class of single-stranded RNAs with closed loop structures formed by covalent bonds of head and tail. Exploration of circRNAs is continually increasing; however, their functional relevance largely remains to be elucidated. In general, they are stable, abundant, conserved and expressed in tissue-specific manner. These distinct properties and their diverse cellular actions indicate that circRNAs modulate transcription and translation, and may even function as translation templates. Growing evidence reveals that circRNAs contribute to various physiological and pathological processes, including the initiation and progression of cancer. In this review, we present the current knowledge about circRNAs in cancer development, as well as their potential for use as biomarkers and even therapeutic targets. CircRNA's role in immune regulation and antitumour immunotherapy is also discussed. In addition, possible challenges in antitumour therapy are raised, and current progress and future perspectives are provided. [ABSTRACT FROM AUTHOR]

Introduction: Experiencing elevated stress increases the risk of further difficulties in mental health, including externalizing behaviors in adolescents. There is a need for understanding the factors that help adolescents mitigate social stress to prevent problematic externalizing behaviors. Growth mindset has been found to be a promising protective factor in adolescent development including mental health. This study aimed to examine the potential buffering role of growth mindset of thoughts-emotion-and-behaviors in the context of two types of social stress (i.e., family and peer) to reduce externalizing behaviors among adolescents.Methods: The sample is composed of 399 adolescents (age range 14-18 years, M = 16.22, SD = 1.21; 56.4% female, 42.3% male, 1.3% gender-nonconforming or variant) from a large, urban, diverse high school in the United States. Adolescent self-reports were administered at one time.Results: Moderation analyses revealed that growth mindset of thoughts, emotion, and behaviors moderate the association between family stress and externalizing behaviors. The magnitude of this association weakened as the level of growth mindset increased, supporting the buffering effect of the moderator. Neither the moderation effect of growth mindset nor the main effects of peer stress and growth mindset were significant in predicting externalizing behaviors.Conclusion: Taken together, the findings suggested that growth mindset has protective effects in reducing externalizing behaviors when adolescents experience family stress. Future directions and implications are discussed. [ABSTRACT FROM AUTHOR]

This study examines how and when entrepreneurial orientation (EO) enhances alliance performance by considering knowledge integration as a critical mediator and bonding as an important contingency of the mediation effect. We test this moderated mediation framework using a sample of 410 alliance firms (based on a total of 820 respondents). Our results show that knowledge integration fully mediates the relationship between EO and alliance performance and that this indirect effect is stronger at a high level of bonding. Overall, we contribute to the entrepreneurship literature by revealing how firms translate their EO‐based strategies into positive alliance outcomes through knowledge integration and when this indirect effect is most effective. [ABSTRACT FROM AUTHOR]

Depending on the reactant property and reaction mechanism, one major regioisomer can be favored in a reaction that involves multiple active sites. Herein, an orthogonal regulation of nucleophilic and electrophilic sites in the regiodivergent hydroamination of isoprene with indazoles is demonstrated. Under Pd‐hydride catalysis, the 1,2‐ or 4,3‐insertion pathway with respect to the electrophilic sites on isoprene could be controlled by the choice of ligands. In terms of the nucleophilic sites on indazoles, the reaction occurs at either the N1‐ or N2‐position of indazoles is governed by the acid co‐catalysts. Preliminary experimental studies have been performed to rationalize the mechanism and regioselectivity. This study not only contributes a practical tool for selective functionalization of isoprene, but also provides a guide to manipulate the regioselectivity for the N‐functionalization of indazoles. [ABSTRACT FROM AUTHOR]

Bidens bipinnata L. is a folk medicinal plant in China that shows significant antihyperlipidemia effectiveness. However, studies of the underlying mechanism study are lacking. In order to explore the potential action sites and the underlying mechanism of treating hyperlipidemic, this work undertook tissue distribution and molecular docking research on the markers of B. bipinnata L., which were obtained through serum pharmacochemistry and network database retrieval. The results showed that seven compounds (gallic acid, protocatechuic acid, rutin, hyperoside, bipinnate polyacetylenicloside, luteolin and quercetin) were screened out as markers. Owing to the diversity of chemical structures, they exhibited an inconsistent trend in tissue distribution. However, all of them had high levels in the liver and no specific distribution in other tissues. More interestingly, seven proteins—HMGCR (1HWK), NR3C1 (4P6W), CYP1A2 (2HI4), RXRA (4PP3), CES1 (1MX1), HSD11B1 (2RBE) and CYP1A1 (4I8V)—showed significant binding affinity with three or more markers, suggesting that they may be the target proteins of B. bipinnata L. This study preliminarily sheds light on the tissue distribution and targets of B. bipinnata L., providing some useful information on the underlying mechanisms of the antihyperlipidemia effect. [ABSTRACT FROM AUTHOR]

Circular RNAs (circRNAs) have been associated with lung cancer (LC), one of the most common cancers, but the underlying molecular mechanisms of the specific correlation with LC carcinogenesis remain unveiled. Quantitative real‐time polymerase chain reaction was applied to examine the level of circZNF609. LC cells were transfected with silenced circZNF609 by siRNAs, and cell proliferation, migration, and apoptosis were evaluated to reflect the influences of circZNF609 knockdown in LC. Biotin‐coupled circRNA capture, FISH and luciferase reporter assays were performed to study the relationship between circZNF609 and miR‐142‐3p. In current work, it was discovered that circZNF609 functioned as an onco‐circRNA, which exhibited high expression as well as facilitated the proliferation and migration in LC cells. Next, we discovered that FUS RNA‐binding protein, which could bind to the ZNF609 pre‐mRNA, induced circZNF609 formation, and increased circZNF609 expression in LC. Furthermore, circZNF609 was verified to sponge and sequester miR‐142‐3p; circZNF609 enhanced LC cell proliferative and migrative ability via targeting miR‐142‐3p. Finally, G protein subunit beta 2 (GNB2) was figured out to involve in circZNF609/miR‐142‐3p axis‐induced LC development. Conclusively, the results indicated that FUS‐induced circZNF609 exerts promotional effects on LC cell proliferation and migration through modulation of the miR‐142‐3p/GNB2 axis, which could offer new insight for understanding LC. [ABSTRACT FROM AUTHOR]

The high fractional free volume (FFV) endowed polymers of intrinsic microporosity (PIMs) with high gas permeability but low selectivity. Herein, an intermediate temperature range was deliberately utilized to tune PIM‐1 membrane microstructure in nitrogen atmosphere to enhance gas separation performance. During intermediate thermal manipulation, the synergistic effects of thermal‐induced cross‐linking and decomposition on PIM‐1 membranes have optimized the micropores for significantly increasing membrane molecular‐sieving ability with the boosted selectivity of 350 (H2/N2), 1,472 (H2/CH4), 3,774 (H2/C3H8), and 197 (CO2/CH4) respectively, with the H2 permeability of 234 Barrer, correspondingly, surpassing the "Robeson's Upper Bound". The facile strategy simultaneously utilizing the thermal‐induced cross‐linking and decomposition, might provide a new platform to develop the high‐performance membranes for highly‐efficient hydrogen purification and CO2 separations. [ABSTRACT FROM AUTHOR]

Dearomative annulation of indoles has emerged as a powerful tool for the preparation of polycyclic indoline‐based alkaloids. Compared with well‐established methods towards five‐membered‐ring‐fused indolines, the six‐membered‐ring‐fused indolines are rarely accessed under thermal conditions. Herein, a dearomative [4+2] annulation between different indoles is developed through an electrochemical pathway. This transformation offers a remarkably regio‐ and stereoselective route to highly functionalized pyrimido[5,4‐b]indoles under oxidant‐ and metal‐free conditions. Notably, this electrochemical approach maintains excellent functional‐group tolerance and can be extended as a modification tactic for pharmaceutical research. Preliminary mechanism studies indicate that the electrooxidation annulation proceeds through radical–radical cross‐coupling between an indole radical cation and an N‐centered radical generated in situ. [ABSTRACT FROM AUTHOR]

Objective: Impairment of basal ganglia (BG)‐thalamo‐cortical circuit causes various symptoms of Parkinson's disease (PD). We investigated the functional connectivity (FC) patterns of putamen among PD subtypes and healthy control (HC) and explored their clinical significance. Methods: A total of 16 patients with tremor‐dominant (TD) PD, 23 patients with postural instability and gait difficulty‐dominant (PIGD) PD, and 31 HC that underwent functional magnetic resonance imaging were observed. Voxel‐wise FC analysis was performed by computing correlation between bilateral putamen and other voxels within the brain. Correlation analysis was performed between FC strength and clinical symptoms. Results: Compared with PIGD group, TD group showed increased FC between left putamen and right cerebellum lobule VI and cerebellum crus I, then we compared the cerebellum FC difference among the three groups. The cerebellum lobule VI FC difference was mainly involved in motor related cortex, and the cerebellum crus I FC difference was related to cognition areas. While compared with HC, TD and PIGD groups both had significant FC difference brain areas correlated with motor and cognition symptoms. The connectively of putamen and right cerebellum lobules VI and I showed positive correlation with tremor and Montreal Cognitive Assessment degree of scores, respectively. The connectivity of putamen and sensorimotor cortex had negative correlation with PIGD scores. Conclusions: The altered connectivity of BG‐cortical circuit in patients with PD was related to PIGD symptoms. Motor and cognitive impairments declined slower in patients with TD PD, which may be related to increased functional connectivity between putamen and cerebellum. [ABSTRACT FROM AUTHOR]

Rationale: Gelsemium elegans Benth. belongs to the family Loganiaceae and is widely distributed in northern America, east Asia, and southeast Asia. It has attracted wide attention for its diverse biological effects and complex architectures. Gelsevirine is one of the major components in G. elegans. Compared with other alkaloids from G. elegans, gelsevirine exhibits equally potent anxiolytic effects but with less toxicity. However, the metabolism of gelsevirine has not been clearly elucidated. Methods: The metabolism of gelsevirine was investigated using liver S9 fractions derived from rat liver homogenates by centrifugation at 9000 g. A rapid and accurate high‐performance liquid chromatography/quadrupole‐time‐of‐flight mass spectrometry (HPLC/QqTOF‐MS) method was applied to characterize the gelsevirine metabolites. Results: We discovered a total number of four metabolites of gelsevirine. The metabolic pathways of gelsevirine consisted of hydrogenation, N‐demethylenation and oxidation in rat liver S9. Conclusions: This is the first study on the metabolism of gelsevirine. We proposed possible metabolic pathways of gelsevirine. These findings may warrant future studies of the in vivo metabolism of gelsemine in animals. [ABSTRACT FROM AUTHOR]

Mitochondrial transcription factor A (TFAM) is a key regulator of mitochondria biogenesis. Previous studies confirmed that reduced TFAM expression sensitized tumours cells to chemical therapy reagents and ionizing irradiation (IR). However, the underlying mechanisms remain largely unknown. In this study, we identified that decreased expression of TFAM impaired the proliferation of tumour cells by inducing G1/S phase arrest and reducing the expression of E2F1, phospo‐Rb, PCNA and TK1. Furthermore, we proved that knockdown of TFAM enhanced the interaction between p53 and MDM2, resulting in decreased expression of p53 and the downstream target TIGAR, and thus leading to elevated level of mitochondrial superoxide and DNA double‐strand break (DSB) which were exacerbated when treated the cell with ionizing radiation. Those indicated that knockdown of TFAM could aggravate radiation induced DSB levels through affecting the production of mitochondria derived reactive oxygen species. Our current work proposed a new mechanism that TFAM through p53/TIGAR signalling to regulate the sensitivity of tumour cells to ionizing radiation. This indicated that TFAM might be a potential target for increasing the sensitization of cancer cells to radiotherapy. [ABSTRACT FROM AUTHOR]

When the image position measuring method is applied to mover position measurement of a linear motor, the selection of the target source image is crucial to measurement accuracy and anti‐interference capacity. In this study, the fence image is used as the original shooting surface, and different kinds of fence images are constructed according to their width standard deviation and grey gradient sum. First, the relationship between the fence image spectrum energy and the correlation of image pixels is analysed. Then the image spectrum energy is used to characterise the feature of fence images during screening to select an aperiodic fence image with a strong anti‐interference. Second, the phase singularity method is used to match the two images before and after displacement to obtain a sub‐pixel displacement value of two images. Finally, according to the system calibration coefficient, the actual displacement value of the linear motor mover is obtained. Experimental results show that the fence image screening from image spectrum energy exhibits more anti‐interference for the position measurement of the linear motor, and the measurement algorithm based on the phase singularity algorithm is more stable. The absolute error of displacement is <0.023 mm, and the relative error change within 0.054–0.862%. [ABSTRACT FROM AUTHOR]

We tested the hypothesis of school satisfaction being an antecedent to social coping behaviors in early adolescents (N = 892) using a two‐wave cross‐lagged panel design. We also explored the possible bidirectional relations between school satisfaction and social coping behaviors. Four types of social coping behaviors in peer conflict contexts included social support seeking, self‐reliance, internalizing behaviors, and externalizing behaviors. The findings showed that school satisfaction significantly predicted three of the four coping behaviors (social support seeking, self‐reliance, and externalizing behaviors) in the expected directions. Among all the social coping behaviors, only social support seeking significantly predicted school satisfaction over time. Taken together, the results suggest that school satisfaction may be an antecedent that predicts most social coping behaviors in early adolescents. Furthermore, the relations between social support seeking coping and school satisfaction appeared to be reciprocal. Implications, especially the importance of monitoring students' school satisfaction, are discussed. [ABSTRACT FROM AUTHOR]

An efficient trifluoromethylthiolation‐based vicinal bifunctionalization of indoles with sodium trifluoromethanesulfinate in the presence of phosphorus oxychloride were achieved to introduce a chlorine atom into the 2‐position under transition‐metal‐free and mild conditions. The protocol could also extend to other sodium perfluoroalkanesulfinates or phosphorus oxybromide. The possible pathway was proposed based on the experimental results. [ABSTRACT FROM AUTHOR]