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Orchardgrass ( Dactylis glomerata L.) is a long-lived, cool-season forage grass that is commonly used for hay production. Despite its economic importance, orchardgrass genome remains relatively unexplored. In this study, we used Illumina RNA sequencing to identify gene-associated molecular markers, including simple sequence repeats ( SSRs) and single nucleotide polymorphisms ( SNPs), as well as heat stress-induced differentially expressed genes ( DEGs) in two orchardgrass genotypes, 'Baoxing' (heat resistant) and '01998' (heat susceptible). Approximately 163 million high-quality trimmed reads were generated from 207 million raw reads using the Illumina HiSeq 2000 platform. A total of 126 846 unigenes were obtained after de novo assembly of the trimmed reads, and 40 078 unigenes were identified as coding sequences ( CDSs). Based on the assembled unigenes, 669 300 high-quality SNPs, including 416 099 transitions and 257 736 transversions, were contained in 75 875 unigenes. In addition, a total of 8475 microsatellites were detected in 7764 unigenes. When placed under heat stress, the total number of DEGs in 'Baoxing' (3527) was higher than in '01998' (2649), indicating that in comparison with heat-susceptible '01998', heat-resistant 'Baoxing' seems to have more unigenes that respond to heat stress. The high-throughput transcriptome sequencing of orchardgrass under heat stress provides useful information for gene identification and for the development of SNP and SSR molecular markers. The comparison of DEGs under different periods of heat stress allowed us to identify a wealth of candidate DEGs that can be further analysed in order to determine the genetic mechanisms underlying heat tolerance in orchardgrass. [ABSTRACT FROM AUTHOR]

Heterogeneous network is introduced to improve the network capacity in LTE Release 9 and system beyond. The potential traffic congestion due to increased users can be alleviated by the cooperation between macro‐cell and pico‐cell. However, the inter‐cell interference caused by RF signal from macro‐cell will reduce the performance severely. Enhanced inter‐cell interference coordination is proposed in Rel. 10 to solve this problem using almost blank subframe (ABS). Yet, the cell specific reference signal in ABS can still cause interference to the data resource element (RE) from the pico‐cell inevitably for non‐colliding scenario. In this Letter, a novel interference cancellation (IC) algorithm is proposed to mitigate the interference. First, the timing and carrier frequency offset of interference signal is estimated and compensated. Second, the interfering channel response is estimated by utilising the channel statistics. Third, the interference signal is reconstructed based on the channel estimation and cancelled in the received signal in time domain. The experiment results show that the performance of proposed IC algorithm is robust. [ABSTRACT FROM AUTHOR]

Pea plants (Pisum sativum L. cv. Greenfeast) were grown and exposed to supplementary UV-B radiation from day 17 after planting under growth cabinet conditions. The effects of this exposure on the ultrastructure of chloroplasts and the total soluble sugar and starch concentrations were estimated. Supplementary UV-B radiation was shown to damage the structure of chloroplasts, as manifested by dilation of thylakold membranes, a progressive disruption of the thylakoid structure and disintegration of the double membrane envelope surrounding the chloroplast, accompanied by the accumulation of large starch grains. Diurnal changes observed in starch concentration suggest that the higher concentration of starch in supplementary UV- B-treated leaves is due to its immobilization, rather than to any increase in starch synthesis: soluble sugars accumulated and remained at a higher level and then later declined. [ABSTRACT FROM AUTHOR]