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The determination of conditions for the reproducible growth of well diffracting crystals is a critical step in every biocrystallographic study. On the occasion of a new structural biology project, several advanced crystallogenesis approaches were tested in order to increase the success rate of crystallization. These methods included screening by microseed matrix screening, optimization by counter‐diffusion and crystal detection by trace fluorescent labeling, and are easily accessible to any laboratory. Their combination proved to be particularly efficient in the case of the target, a 48 kDa CCA‐adding enzyme from the psychrophilic bacterium Planococcus halocryophilus. A workflow summarizes the overall strategy, which led to the production of crystals that diffracted to better than 2 Å resolution and may be of general interest for a variety of applications. Advanced crystallogenesis methods, including microseed matrix screening, crystallization optimization by counter‐diffusion and crystal detection by trace fluorescent labeling, were used in combination to successfully produce various diffracting crystal forms of a CCA‐adding enzyme from the psychrophilic bacterium Planococcus halocryophilus. [ABSTRACT FROM AUTHOR]