1. TbEu(BTC) as a dNTP label for LAMP outcome in nucleic acid testing.
- Author
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Gao, Chenfeng, Wong, Joseph J. Y., and Hall, Elizabeth A. H.
- Subjects
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METAL-organic frameworks , *LAMPS , *NUCLEIC acids , *DETECTION limit - Abstract
Lanthanide metal organic frameworks (MOFs), Eu(BTC) and TbEu(BTC) were assessed to follow uptake of deoxynucleotide triphosphate (dNTPs) in loop mediated isothermal amplification (LAMP) using BST2.0. dNTPs could be determined below 150 μM. A ratiometic measurement of Tb3+:Eu3+ fluorescence in TbEu(BTC) gave a limit of detection of 2.08 μM for dNTPs. dNTP uptake slowed above 8 mM Mg2+. This correlated with end‐point and real‐time LAMP, using PicoGreen and SYBR green respectively. For the P.Mal‐Lau 18S rRNA primer set, with P. malariae a detection limit of 102.5 copies was obtained. Cross reactivity between P. malariae and P. knowlesi was shown with 18S rRNA primer sets. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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