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A "Janus particle" refers to the production of two materials in a single system and shows a difference in physical characteristics, and two surfaces participate in the formation with different chemistries. This research generated the Janus using a hybrid of zinc oxide (ZnO) and gold (Au) on the sensor surface toward making high‐performance DNA sensors. The Janus ZnO/Au‐textured film was synthesized via the one‐step sol–gel method, which involves a suitable ratio of a mixture of ZnO sol seed solution. The synthesized Janus ZnO/Au‐textured film undergoes a low‐temperature aqueous hydrothermal route to synthesize quasi‐one‐dimensional nanowires. The average grain size in the Janus ZnO/Au nanotextured wire was 41.60 nm. The fabricated nanotextured wire was further optimized by tuning the thickness and characterized by XRD and high‐resolution microscopy. Electrical characterization was conducted on the Janus ZnO/Au nanotextured wire coupled with an interdigitated electrode sensor to detect the specific leptospirosis DNA strand. The generated device is capable of detecting lower DNA concentration at 1 × 10–13 M with a sensitivity of 8.54 MΩ M–1 cm–2. The high performance is attained on linear concentrations of 10–6–10–13 M with the determination coefficient, "I = 135437.63C−3609.07" R2 = 0.9551. A potential strategy is proposed as a base for developing different high‐performance sensors. [ABSTRACT FROM AUTHOR]